by serology. In the last 2 years migration of workers from endemic area from North Karnataka has resulted in upsurge of Brucellosis in this area. So evaluation of all PUO for brucellosis was done for 2 years. There was laboratory associated infection among 4 postgraduate students who worked with blood culture automated system. Methods: During 2 years duration 841 cases of PUO were admitted to Kasturba Hospital Manipal and evaluated. 32 cases were diagnosed as Brucellosis by isolation in blood culture, standard tube agglutination test and ELISA for IgM and IgG. Results: Out of 32 cases 15 were blood culture positive and 17 were positive only by serology. 15 blood culture positive cases also had very high antibody titer in tube agglutination test in the range of 320 to 2560 international units for all the patients. Conclusion: PUO due to brucellosis are increasingly being reported in this area which was previously not encountered. Brucellosis has to be kept in mind for all PUO cases. Due care has to be taken in laboratory while handling clinical specimens.