The fish fauna diversity and distribution patterns were investigated in the Rana stream situated in the Mandi district, Himachal Pradesh, India. Given the considerable variations in altitude and climatic conditions within the study area, a stratified systematic sampling approach was adopted. The stream was stratified into two zones, upstream and downstream, based on the altitudinal gradient. A total of five fish species were identified, representing 4 genera and 2 orders, namely Cypriniformes and Channiformes. Cypriniformes emerged as the predominant order between the two. The Spearman correlation between fish species and selected abiotic parameters of stream water was examined. Several diversity indices, including Simpson's index, Simpson's diversity index, the Shannon index of diversity, Pielou's evenness index, and Margalef's richness index, were employed to evaluate fish diversity in the stream. The Water Quality Index (<i>WQI</i>) values varied from 49.65 to 55.14, representing that water quality spanning from &#171;good&#187; to &#171;poor&#187;. Species diversity was found to be higher in the downstream altitudinal zone compared to the upstream zone. Similarly, microhabitat pools exhibited greater species diversity. It has been found that the low gradient and slow current velocity in downstream locations leading to increased nutrient deposition in deep pools thereby favoured higher fish species abundance.
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