Evaluating and integrating local ecological knowledge held by fishers into decision-making processes has the potential to significantly enhance fisheries management. The present study aimed to collect information on fishery practices and to assess the ecological knowledge of local professional fishers through interviews, evaluating its importance in managerial design. As a case study, Polyphytos Reservoir in Greece, which supports substantial fisheries, was selected. During the summer of 2023, thirty-seven interviews were conducted to document fishing efforts, methods, catches, biological information, and fishers’ perspectives on lake management, and economic/demographic details. In addition to gathering data on fishing activities, the study seeks to understand fishers’ perspectives on managerial deficiencies and necessities, thereby revealing their valuable ecological knowledge. The integration of this knowledge into decision-making processes can empower stakeholders and enhance local participation in fishery management. Ultimately, this approach has the potential to address long-standing conflicts, foster inclusive processes, and ensure better collective outcomes.