The purpose of this study is to develop a laboratory safety leadership measurement tool. In this study, safety leadership was defined as a series of actions in which laboratory managers establish safety plans based on safety communication with research workers based on their interest and belief in safety, and all laboratory members establish the importance of safety. This measurement tool was finally developed through the stages of literature review, factor and item development, and validity analysis. A survey was conducted to confirm the validity of the measurement tool, and a total of 404 copies were analyzed. As a result of the validity verification, the safety leadership measurement tool consisted of 14 questions of four factors, and the four factors were safety communication, safety interest, safety belief and planning, safety behavior, and participation. For each question, a 6-point Likert scale was used.