Management Of Burn Injuries Research Articles

Strategies for Optimizing Acute Burn Wound Therapy: A Comprehensive Review

Recent advancements in acute burn wound therapy are transforming the management of burn injuries, with a focus on improving healing times, graft integration, and minimizing complications. However, current clinical treatments face significant challenges, including the difficulty of accurately assessing wound depth and tissue viability, which can lead to suboptimal treatment planning. Traditional closure methods often struggle with issues such as delayed wound closure, limited graft survival, inadequate tissue regeneration, and insufficient vascularization. Furthermore, managing infection and minimizing scarring remain persistent obstacles, impacting functional recovery and aesthetic outcomes. Key areas of innovation include advanced imaging techniques that enable more precise assessment of wound depth, size, and tissue viability, allowing for more accurate treatment planning. In addition, new closure strategies are being developed to accelerate wound closure, enhance graft survival, and address challenges such as tissue regeneration, vascularization, and infection prevention. These strategies aim to optimize both functional recovery and aesthetic outcomes, reducing scarring and improving the quality of life for burn patients. While promising, these emerging techniques require further research and clinical validation to refine their effectiveness and expand their accessibility. Together, these innovations represent a significant shift in acute burn care, offering the potential for more personalized, efficient, and effective treatments.

Investigation of the antimicrobial properties of temperature-sensitive hydrogel containing silver sulfadiazine against various bacterial strains

In burn wound management, infection poses a significant challenge, accounting for 75% of deaths in burn patients. Silver sulfadiazine is broadly used as an effective antibacterial agent for treating burns. Numerous researchers have explored various dosage forms of silver sulfadiazine, such as cream, ointment, topical spray, and hydrogel, for antimicrobial topical applications. Hydrogels offer appealing advantages over conventional drug delivery systems due to their sensitivity and responsiveness to stimuli, particularly temperature. Nevertheless, the comprehensive investigation of the antimicrobial properties of temperature-sensitive hydrogel containing silver sulfadiazine against different bacterial strains remains lacking. Thus, the main objective of the current study is to explore the antimicrobial properties of the temperature-sensitive hydrogel, incorporating silver sulfadiazine, against various bacterial strains colonized in burn wounds. To assess the antimicrobial activity of the temperature-sensitive hydrogel, inhibition zone diameters were measured against different types of Gram-positive strains (Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Streptococcus pyogenes) and Gram-negative strains (Escherichia coli and Klebsiella pneumoniae). The synthesized silver sulfadiazine-loaded temperature-sensitive hydrogel exhibited remarkable antimicrobial efficacy against these bacteria. Notably, there was no significant difference in the inhibition zone diameter between the silver sulfadiazine-loaded temperature-sensitive hydrogel and the positive control (p>0.05). These findings affirm that the silver sulfadiazine-loaded temperature-sensitive hydrogel holds promise as a drug delivery medium, demonstrating excellent antimicrobial activity against various bacterial strains that colonized in burn wounds.

Electroactive Asymmetric Dressing for Spatiotemporal Deep Burn Scarless Healing and Management.

Burn care and treatment differ markedly from other types of wounds, as they are significantly more prone to infections and struggle to maintain fluid balance post-burn. Moreover, the limited self-healing abilities exacerbate the likelihood of scar formation, further complicating the recovery process. To tackle these issues, an asymmetric wound dressing comprising a quercetin-loaded poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB@Qu) hydrophilic layer and a zinc oxide nanoparticle-loaded, thermally treated polyvinylidene fluoride (HPVDF@ZnO) hydrophobic layer is designed. This dressing provided antibacterial property and exudate management in the early stages of burn treatment, preventing infection and maintaining moisture balance at the wound site. As healing progresses, the electroactive properties of HPVDF@ZnO and quercetin from P34HB@Qu synergistically regulate cell migration and differentiation, accelerating wound healing and facilitating scar-free regeneration. Furthermore, the wound dressing assisted in the regeneration of skin appendages. This study underscores the full-cycle strategy of versatile wound dressings for spatiotemporal burn wound management from injury to scarless healing.

Exploring the gaps: A scoping review of burn injury research in skin of colour.

Burn injury management and outcomes reveal observed disparities in individuals with darker skin tones, likely influenced by limited representation in medical literature and clinical research. These gaps may contribute to variations in care quality and outcomes for these populations. A comprehensive literature search was conducted across PubMed, Scopus, and Embase databases, initially yielding 74 articles. Due to limited relevant studies directly addressing the research question, the approach shifted from a systematic review to a scoping review to allow for a broader exploration of potential disparities in burn injury outcomes. Following these criteria, 31 relevant articles were identified and analysed. The analysis suggests an underrepresentation of diverse skin tones in medical textbooks and clinical research, limitations in current burn assessment tools for darker skin, and a lack of tailored treatment protocols. Studies indicate that patients with darker skin tones may face higher risks of complications and varied outcomes, potentially influenced by systemic healthcare challenges and limited guidelines addressing diverse skin types. This scoping review highlights the importance of more inclusive research and clinical practices that consider the specific needs of individuals with darker skin tones. Addressing these observed gaps can support improvements in burn injury management, ultimately contributing to more equitable healthcare for all skin types.

Could Fibrin Sealants (TISSEELTM) Be Effective in the Management of Burn Injuries? A Histopathological Study in Rats.

Background: Burn injuries remain a major clinical problem worldwide, which require special management by experienced plastic surgeons. However, they cannot be available in every healthcare unit; consequently, there is a need for effective treatment options that could be utilized by a wide range of non-expert healthcare professionals. The aim of the present experimental study was to investigate the safety and efficacy of using a fibrin sealant (TISSEELTM) compared to the conventional treatment with sulfadiazine on partial-thickness burn in a rat animal model. Methods: A cohort of Sprague Dawley rats underwent partial-thickness contact thermal burn wounds and were divided into three study groups: control group (no treatment), silver sulfadiazine cream group and TISSEELTM group. Following animal sacrifice, a blinded histopathologic analysis was conducted regarding inflammatory response, healing and tissue regeneration. Results: In total, 30 animals were included with a median weight of 236 ± 10 g. Two animals from the control group died on the first postoperative day. Animals in the TISSEELTM group presented dominant collagen expression compared to animals in the control and silver sulfadiazine cream group (p = 0.000). Histopathologic analysis also demonstrated marked leukocyte infiltration (p = 0.009), increased neovascularization (p = 0.000) and higher fibroblast expression (p = 0.002) in the TISSEELTM group compared to the other two groups. Conclusions: TISSEELTM seems to be a safe alternative (or even principal) option for the initial therapeutic approach of partial-thickness burn injuries. Moreover, it seems to be superior to silver sulfadiazine in terms of tissue healing and regeneration. However, additional experimental as well as clinical research is necessary prior to implementation in clinical practice.

Herbal Remedies in Burn Wound Management: A Comprehensive Review

This study focuses on the formulation and evaluation of a herbal burn healing cream using Jatropha curcas latex extract. The cream combines Jatropha curcas latex with compatible excipients to optimize stability, texture, and therapeutic efficacy. Various physicochemical, rheological, and pharmacological evaluations were conducted to assess its suitability for burn wound management. The cream demonstrated desirable pH, viscosity, spreadability, and consistency, along with significant antimicrobial activity against common wound pathogens. The formulation aims to harness the anti-inflammatory, antimicrobial, and wound healing properties of Jatropha curcas latex for effective burn treatment. Keywords: Healing Cream, Rheological, Anti-Microbial, Anti-Inflammatory

A Comparative Study Between Amniotic Membrane and Hydrocolloid in the Healing of Partial Thickness Burn Wounds

Background: Management of partial thickness burn wounds is mostly conservative, and rapid wound healing is desirable to obtain a good functional and cosmetic outcome. Wound dressing materials play an important role in maintaining an optimal wound milieu and avoiding complications from delayed healing. Methods: From January 2018 to December 2018, the study was conducted as a clinical trial over 100 patients at Dhaka Medical College Hospital. The patients who sustained partial thickness burn < 15% TBSA were included in the study, equally allocated between two (2) comparison groups (Group A: amniotic membrane and Group B: hydrocolloid), and the clinical efficacy and functionality of the two groups regarding the rapidity of wound healing, frequency of dressing changes, requirement of analgesics, rate of infections, and need for a skin graft were assessed. Results: Hydrocolloid dressing showed a shorter healing time (p<0.002), less frequent dressing change (p<0.001), lower requirements of pain medication (p<0.001), and improved patients comfort which were statistically significant. Conclusion: Amniotic membrane showed comparable clinical and functional efficacy with hydrocolloid in burn wound healing, with superior healing time and comfort in favor of the later. J Com Med Col Teachers Asso July 2024; 28(2): 58-64

An overview on the application of nanotechnology in burn wound management

An overview on the application of nanotechnology in burn wound management

Innovative surgical management of large burn wounds in patients with elevated body weight to enhance team safety

Over the past four decades, obesity rates in the United States have consistently increased, with current estimates indicating that over 42% of adults are affected [1]. In contrast, burn injuries have decreased significantly during the same period, largely due to effective public awareness campaigns and improvements of safety regulations [2]. Despite these opposing trends, the percentage of burn patients at the author’s hospital with a concurrent diagnosis of obesity has risen, posing unique challenges for clinical providers. Patients with elevated body weight and extensive burns require special considerations for proper positioning, wound exposure, and minimizing ergonomic risks to healthcare workers [3]. Currently, there is a notable lack of comprehensive guidelines on operative strategies specifically tailored for this patient population. In response to this knowledge gap, the authors’ institution implemented novel surgical techniques designed to manage circumferential burn wounds in patients with higher body weight.

Management of Burn Wound in Diabetic Old Patient with Panchagavya Formulations – A Case Report

ABSTRACT Burn injuries are often prevalent and among the most catastrophic injuries encountered in casualty or surgery departments, involving skin and other tissues. These injuries typically result from heat sources such as solids, liquids, or fire, while scalds arise from hot liquids, electricity, chemicals, cold, friction, or radiation. Despite varying causes, the male-female ratio of burn cases remains consistent. In India, the annual incidence of burns is estimated at six to seven million. Topical antimicrobial agents are crucial for treatment. This case study focuses on an 83-year-old woman with acute burn wounds on her right leg and abdomen, accompanied by pain, discharge, foul odor, edema, and skin discoloration. Treatment involved Panchagavya Chikitsa, utilizing local applications of Gomutra twice daily for wound cleaning and Jatyadi Ghrita twice daily for wound healing over 60 days. Additionally, 15 ml of Gomutra Arka was administered internally twice daily for 15 days. Significant improvements were observed, including complete wound healing and associated symptom resolution. The positive outcomes may be attributed to the local and systemic effects of Gomutra, Jatyadi Ghrita, and Gomutra Arka, which possess antimicrobial, antiseptic, scrapping, and wound-healing properties. The findings suggest that Panchagavya formulations effectively and safely manage burn wounds, even in geriatric patients. Jatyadi Ghrita and Gomutra demonstrated promising results without causing adverse effects in this case.

Fabrication and evaluation of four combinations of pullulan-based biopolymeric, microporous scaffolds impregnated with peppermint oil for management of partial thickness burn wounds and accelerated tissue regeneration

Burn injuries are common in both civilian and military settings and cause severe morbidity and mortality. The present study aimed to develop microporous bioactive scaffolds from the natural polysaccharide, pullulan, combined individually with Gelatin (Gel)/Alginate (Alg)/Chitosan (Ch)/Carboxy-methyl cellulose (CMC), and crosslinked with glutaraldehyde to form a polymeric network. The objective was to evaluate and compare their efficacy in healing second degree partial-thickness burn wounds. The scaffolds were also impregnated with peppermint oil (PEO) to provide potential analgesic and cooling effects. Scaffolds were characterized using FTIR, TGA and SEM analysis to understand the intra-and intermolecular interactions and morphological characteristics. In vitro parameters like fluid uptake ability, biodegradation and hemolytic index were also assessed. Biocompatibility studies, including cell adhesion, migration and proliferation, were conducted using L929 mouse fibroblast cell lines. The in vivo efficacy was evaluated in Sprague Dawley rats inflicted with second degree burns to assess and compare wound retraction across different experimental groups. Histology, immunohistochemistry along with gram and picrosirius staining studies were also performed. The average pore size of all biopolymeric scaffold combinations ranged between 25.6 and 298 μm. All four test combinations were non-hemolytic, with hemolytic indices between 0.3 and 2.9. Wound retraction studies in rats clearly indicated that among the four test combinations studied, Pu/Gel/PEO scaffold not only reduced local inflammation faster but also accelerated dermal regeneration in comparison to positive control as well as other test combinations (viz., Pu/Alg/PEO, Pu/Ch/PEO, and Pu/CMC/PEO). Immunohistochemistry results corroborated animal efficacy findings and demonstrated enhanced expression level of epidermal growth factor and fibroblast growth factor-2 in Pu/Gel/PEO scaffold treated group. Picrosirius staining also revealed enhanced collagen deposition in Pu/Gel/PEO scaffold treated group. The study findings suggest that biopolymeric scaffolds made from pullulan combined with gelatin and PEO (Pu/Gel/PEO) could serve as a promising substrate for skin regeneration template for second degree burns.

The efficacy of cellulose dressings in burn wound management: a systematic review and meta-analysis

IntroductionBurn wound management is challenging especially in paediatric patients when optimising outcomes. Superficial burns are generally managed conservatively with dressings however frequent dressing changes can be a source of pain and discomfort. Cellulose dressings mitigate these caveats and current reports within the literature have demonstrated positive outcomes when compared against routine dressing types. The authors aim to report a systematic review and meta-analysis on the use of cellulose based dressings in burn wound management. MethodsThis review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) statement standards. The primary outcome measures were epithelialization rate, duration of hospital stay and the number of dressings changes. The electronic databases of MEDLINE, EMBASE, CINAHL, google scholar, Pubmed and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched. OpenMeta [analyst] software was used for data synthesis. ResultsEleven studies were included in the qualitative synthesis with eight articles meeting the inclusion criteria for quantitative analysis with cellulose based dressings showing a significantly increased rate of healing on mean difference analysis:1.350 (0.007, 2.694), standard error; 0.685, P value = 0.049. A shorter duration of hospital stay was also observed in the cellulose cohort: 6.583(5.739, 7.428), standard error 0.431, P-value < 0.001 as well as the frequency of dressing changes which was lower: odds ratio 0.125 (0.015, 1.062) P-value < 0.001. There was no difference in the infection rate compared to standard dressings ConclusionCellulose dressings can expedite wound healing whilst reducing the duration of hospitalisation and frequency of dressing changes compared to standard dressing in burn wound management. The authors however recommend further high quality trials to enhance the current evidence base.

A carboxymethyl chitosan and dextran hydrogel with slow and rapid photothermal conversion for sequential promoting burn wound healing and inhibiting scar proliferation

Facilitating swift burn wound healing while effectively preventing scar formation continues to be a considerable challenge in medical practice. In this study, an injectable carboxymethyl chitosan/oxidized dextran/polyvinylpyrrolidone/dopamine (COPD) hydrogel was designed for the effective sequentially promotion of burn wound healing and inhibition of scar formation. The COPD hydrogel precursor solution was injected into the burn wound via a double-barreled syringe and transformed into an adherent hydrogel within 25 s. The inclusion of dopamine imparted good free radical scavenging properties to the hydrogel. In particular, the gradual oxidation of dopamine to polydopamine enabled a unique heat production pattern—initially slow (photothermal conversion efficiency: 30.3 %) and then rapidly temperature increasing (photothermal conversion efficiency: 42.8 %) —under single laser irradiation. The effect of promoting healing at the initial stage of the wound was evaluated by constructing a male C57BL/6 mice model with deep second-degree burns, observation of the wound area, PCR analysis, and immunohistochemical staining. Furthermore, the scar inhibition was confirmed by observing reduced expression levels of α-SMA and COLI, along with a decreased collagen I/III ratio. With tunable mechanical properties (maximum compressive strength of 966.4 ± 51.7 kPa), the COPD hydrogel holds significant promise as an adjunctive photothermal platform for intelligent burn wound management.

Bromelain based enzymatic debridement in burn patients, its outcome, advantages and disadvantages

The aim of this study is to evaluate the outcomes, advantages, and disadvantages of bromelain-based enzymatic debridement in the management of burn wounds. Ours is a study in which we have selected 10 patients with deep dermal and full thickness burns admitted in SMS hospital, Jaipur. These patients underwent bromelain based enzymatic debridement applied as per the manufactures protocol. The patients were then observed for bromelain’s efficacy in debridement, wound healing, pain, duration of hospital stay, superadded bacteraemia and cosmetic outcome. Complete removal of burn eschar was observed in 9 out of 10 patients. Bromelain-based enzymatic debridement effectively removed necrotic tissue within a shorter timeframe compared to traditional methods. Patients treated with bromelain-based enzymatic debridement showed significantly faster wound healing, reduced infection rates, and lower pain levels during debridement. Improved cosmetic outcomes, such as reduced scarring and better skin texture, were also observed. Bromelain-based enzymatic debridement offers notable advantages in burn care, including expedited healing and enhanced patient comfort. Despite these benefits, the treatment's higher cost and the risk of allergic reactions are important considerations. The limited availability of bromelain-based products also poses a challenge. These factors suggest that while bromelain is a promising alternative, its use should be carefully evaluated based on individual patient needs and resource availability.

On-site burn severity assessment using smartphone-captured color burn wound images

Accurate assessment of burn severity is crucial for the management of burn injuries. Currently, clinicians mainly rely on visual inspection to assess burns, characterized by notable inter-observer discrepancies. In this study, we introduce an innovative analysis platform using color burn wound images for automatic burn severity assessment. To do this, we propose a novel joint-task deep learning model, which is capable of simultaneously segmenting both burn regions and body parts, the two crucial components in calculating the percentage of total body surface area (%TBSA). Asymmetric attention mechanism is introduced, allowing attention guidance from the body part segmentation task to the burn region segmentation task. A user-friendly mobile application is developed to facilitate a fast assessment of burn severity at clinical settings. The proposed framework was evaluated on a dataset comprising 1340 color burn wound images captured on-site at clinical settings. The average Dice coefficients for burn depth segmentation and body part segmentation are 85.12 % and 85.36 %, respectively. The R2 for %TBSA assessment is 0.9136. The source codes for the joint-task framework and the application are released on Github (https://github.com/xjtu-mia/BurnAnalysis). The proposed platform holds the potential to be widely used at clinical settings to facilitate a fast and precise burn assessment.

A Paintable Small-Molecule Hydrogel with Antimicrobial and ROS Scavenging Activities for Burn Wound Healing.

Delayed wound healing induced by bacterial infection and a persistent inflammatory response remains a great clinical challenge. Herein, we reported a paintable, anti-bacterial, and anti-inflammatory Nap-F3K-CA (Nap-Phe-Phe-Phe-Lys-Caffeic Acid) hydrogel for burn wound management based on caffeic acid (CA)-functionalized short peptides (Nap-Phe-Phe-Phe-Lys). Hydrogels are assembled by non-covalent interactions between gelators, and the incorporation of CA promotes the self-assembly of the hydrogel. After being applied to burn wounds, the hydrogel effectively adapted to irregular wound beds and maintained a moist protective environment at the wound. The Nap-F3K-CA hydrogel can scavenge ROS to relieve oxidative damage and downregulate proinflammatory levels. The Nap-F3K-CA hydrogel also displayed potent antibacterial activity against Gram-positive and Gram-negative bacteria, which reduced the incidence of wound infections. Moreover, the hydrogel exhibited good biocompatibility and hemostatic function. In vivo experiments demonstrated that the Nap-F3K-CA hydrogel significantly accelerated the repair of the skin structure including promoting collagen deposition, vascular regeneration, and hair follicle formation. These findings proved the clinical application potential of the Nap-F3K-CA hydrogel as a promising burn wound dressing.

Assessment of Moringa oleifera’s Efficacy in Promoting Burn Wound Healing in Wistar Rats: A Preclinical Evaluation

Moringa oleifera [M. olifera] is an indigenous plant of India. It is used in various diseases in different forms. In current study ethanolic extract of bark of M. olifera was used to formulate an ointment with white soft paraffin. Burn wound model was used to evaluate wound healing potential of M. olifera. Second-degree burns were created using a spatula [1 cm diameter] in wistar albino rat. 5 group’s were made, randomly, of wistar rats, i.e. positive control [treated with ointment base], negative control [with no treatment], Test I and II [Ointment at 5% and 10% ethanolic extract of M. olifera bark], Standard drug treated group. Parameters that were used for evaluating the activity were the percentage of wound contraction and histopathology of skin. The result showed dose dependent activity on contraction of wound. In study of histopathology it was observed that period of epithelialization got shortened and significant increase in periods of granulation tissue formation M. oleifera treated groups when compared to untreated control groups. These results represent that the M. olifera can be useful as a excellent therapeutic instrument in the management of burn wounds. Additional characterization is required for identifying the chemical constituents responsible for wound healing.

Fighting A New Front On An Old Battlefield: Examining the Development of Topical Antimicrobial Care to Control Burn Wound Sepsis.

Recognition of invasive burn wound sepsis as a major cause of morbidity and mortality in burn injured patients has profoundly changed the management of burn wounds and its associated complications. The development of effective topical antimicrobial therapy is one of the last major developments of modern burn care and has been driven by major world events and scientific breakthroughs. Topical antimicrobial burn care has evolved from the use of anecdotal remedies to scientific breakthroughs such as Moyer's successful dilution of silver nitrate solution, Fox's described benefit of silver sulfadiazine use in animal models, and Pruitt's dramatic improvement in post-burn mortality using topical mafenide acetate in burn wounds. The objective of this manuscript is to review the definition of burn wound sepsis and highlight the major developments and breakthroughs in topical burn wound care throughout history. This includes historical events like major wars or domestic fires that have influenced or impacted the understanding and treatment of burn wounds. Newer advances in topical antimicrobial care such as nanosilvers and dressing technologies that improve the morbidity and mortality associated with burn wound sepsis and novel approaches to management will also be discussed. To improve burn care, it is prudent to look to the past and learn from the experiences of those who contributed to the control of burn wound sepsis.

A carboxymethyl cellulose/chitosan-based hydrogel harvests robust adhesive, on-demand detachment and self-healing performances for deep burn healing

The irregularities resulting from burns and the discomfort associated with changing dressings during treatment remain challenging to provide effective wound therapy. Hydrogels typically lack the capacity to adhere to tissue and are unable to remain at the wound site for an adequate duration, or fail to detach from tissue when required. In this study, a carboxymethyl cellulose/chitosan-based hydrogel with robust tissue adhesion, self-healing and on-demand detachment was designed via a Schiff-base reaction for deep burn healing. The network structure is self-repairing and does not require external stimulation. The injectable hydrogel fragments undergo self-healing, forming a piece of hydrogel that adapts to the shape of the wound and completely fills the scald wound. The Schiff-base reaction in the hydrogel is formed by the aldehyde group of dialdehyde-modified carboxymethyl cellulose (DCMC) and the amino group of dopamine modified carboxymethyl chitosan (CS-DA), which acts as a dynamic crosslink and endows the hydrogel with self-healing capability and on-demand detachment from tissues by using a benign amino acid solution. Additionally, the incorporation of dopamine has been demonstrated to augment the adhesive properties of the hydrogel. Cytotoxicity assays revealed that the hydrogels exhibit favourable biocompatibility. This research offers a promising approach for the management of burn injuries and represents a significant advancement in the development of cellulose/chitosan hydrogels for biomedical applications.

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.

Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.