Management Of Anthracnose Research Articles

Discovery of natural fungicidal principles from the plant sources for effective management of chilli anthracnose

Anthracnose of chilli causes pre- and post-harvest fruit decay, resulting in significant yield losses. We surveyed major chilli growing regions in India and identified six Colletotrichum sp. viz., Colletotrichum truncatum, C. gloeosporioides, C. siamense, C. fructicola, C. karstii and C. cliviicola based on morphological characteristics and phylogenetic analysis of 115 pure cultures isolated from symptomatic fruits. Antifungal potential of aqueous, acetone, ethyl acetate and methanol extract of 52 plant parts comprising 28 families were evaluated against all identified Colletotrichum sp. Out of these, aqueous extracts (30%) of Syzygium aromaticum, Cinnamomum zeylanicum, Allium sativum and Phyllanthus emblica completely inhibited mycelial growth and conidial germination and solvent extracts (5 mg) showed a clear growth inhibition zone in vitro against Colletotrichum sp. Aqueous extract of S. aromaticum and ethyl acetate extract of C. zeylanicum was strongest as observed shrinking of cell components and maximum zone of inhibition (60 mm) of Colletotrichum sp., respectively.

Molecular characterization of native pink pigmented facultative methylotrophs of chilli and their induced systemic resistance mechanism in management of anthracnose

Aim: This study aimed to assess the plant growth promotional ability and biocontrol efficiency of native pink pigmented facultative methylotrophs (PPFMs) of chilli for management of chilli anthracnose disease through induced systemic resistance mechanism under pot culture experiments. Methodology: The selected native PPFMs were identified by analysis of 16S rRNA gene sequence. These isolates along with reference strain (Methylobacterium extorquens AM1) were inoculated to healthy chilli plants. The pathogen (Colletotrichum capsici) was later inoculated to these chilli plants, and induced systemic resistance (ISR) molecules were estimated at different intervals from one day after challenge inoculation of pathogen (DAI) upto 7 DAI. Influence of PPFM on plant growth parameters, yield, capsaicin content and PPFMs population load on phyllosphere and rhizosphere was studied. Results: The results of 16S rRNA gene sequence analysis revealed selected isolates as Methylobacterium populi (PPFM6) and Methylobacterium radiotolerans (PPFM170). The ISR molecules tested were significantly influenced by PPFM isolates. The peroxidase and phenyl alanine ammonia lyase (PALase) activity increased from 1 DAI to 7 DAI whereas chitinase, polyphenol oxidase (PPO) and phenol contents increased upto 5 DAI, after which gradual decrease was noticed. Inoculation of PPFMs to chilli crop significantly improved plant height, dry matter and chlorophyll content. Significantly lesser disease incidence, more yield and more capsaicin content was observed with application of M. populi as compared to control. Interpretation: The present study highlights native M. populi (PPFM6) of chilli as an effective plant growth promoter, exhibiting significant biocontrol efficiency against C. capsici.

Integrated application of botanicals, fungicides and bio-agents against anthracnose (Colletotrichum truncatum) of Green gram to improve benefit cost ratio

Anthracnose of Green gram become one of the major disease in many countries. An experiment was conducted for two consecutive years to develop an integrated management of anthracnose of green gram using botanical and fungicide and bio agents combinely under natural field conditions with ten treatments. Before selection for field trial the individual treatments were tested for bio-efficacy under in-vitro condition. Results of the field trials it concluded that combinations of single spraying of Propiconazole 25% EC @ 0.1% along with one spraying of garlic oil @ 0.15% with one spraying of T. harzianum @ 4 g gave minimum disease severity (8.20%) with highest grain yield (7.89 q/ha) and highest cost benefit ratio (4.37). The present study demonstrate that the spraying of Propiconazole along with Garlic extract and T. harzianum was effective against anthracnose in field and which is also in affordable limits of the farmers.

Management of blossom blight and anthracnose disease of mango caused by Colletotrichum gloeosporioides

In the present investigation, efforts were made to evaluate different fungicides against blossom blight and anthracnose of mango at different AICRP (F) centres in India. From the five years pooled data, it was observed that carbendazim + mancozeb (0.2%) were the most effective in controlling blossom blight and anthracnose disease of mango at Mohanpur, Rahuri, Rewa and Paria centres. At Sangareddy, on the other hand, chlorothalonil (0.2%) was found to be effective controlling anthracnose of mango. At Vengurle, carbendazim (0.1%) was found to be effective in the management of blossom blight and anthracnose of mango. It is thus concluded that spraying of carbendazim+ mancozeb (0.2%) or carbendazim (0.1%) thrice at 10 days interval, starting from the initiation of the disease symptoms was found to be the most effective for management of blossom blight and anthracnose of mango.

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit.

Anthracnose caused by Colletotrichum fructicola is one of the most important diseases in pear fruit, resulting in huge economic losses. Public awareness of protecting the environment and food safety, together with pathogen resistance to many key fungicides have led to an urgent need to develop alternative strategies for controlling fruit diseases. Here, the antifungal activity of a natural product, dihydromaltophilin [heat-stable antifungal factor (HSAF)], against C. fructicola in vitro and in vivo was investigated to determine its efficacy for anthracnose management. HSAF exhibited pronounced antifungal activity against in vitro mycelial growth of C. fructicola, with a half-inhibition concentration of 0.43 mg L-1 . Hyphae treated with HSAF showed defects such as hyperbranching, swelling and depolarized growth. Conidia germination in the pathogen was inhibited by HSAF in a dose-dependent manner. In the presence of 4 mg L-1 HSAF, conidia germination was significantly delayed, and germ tube growth was inhibited. HSAF at 8 mg L-1 completely blocked conidia germination in C. fructicola. In addition, HSAF disrupted coordination of cytokinesis with growth and nuclear division, induced reactive oxygen species production in conidia, and damaged the integrity of the conidia cell wall. Moreover, an in vivo test confirmed that 50 mg L-1 HSAF significantly reduced the development of anthracnose decay in pear fruit caused by C. fructicola. HSAF was highly effective in reducing pear anthracnose caused by C. fructicola and has great potential to become a new type of fruit preservative.

Essential oils control anthracnose in pepper seeds

Pepper (Capsicum annum) is one of the main vegetables consumed in the Brazilian market, however, an important fungal disease, anthracnose, caused by Colletotrichum gloeosporioides, can decimate all its fruits in the field. Chemical control is the most commonly used measure of control, but, some active ingredients are proving ineffective, and consumers appreciate healthier options such as organic foods. Therefore, studies with natural substances that may help in the management of diseases have been encouraged. Because it is transmitted via seed, seed treatment becomes an essential measure in the management and control of pepper anthracnose. The objective of this study was to evaluate the effect of basil (Ocimum basilicum), citronella (Cymbopogon winterianus), clove (Syzygium aromaticum), copaiba (Copaifera langsdorfii), eucalyptus (Eucalyptus citriodora), mint (Mentha arvensis), rosemary (Rosmarinus officinalis) and tea tree (Melaleuca alternifolia) essential oils on the development of C. gloeosporioides. Citronella, clove, eucalyptus, mint and basil essential oils totally inhibited the pathogen, regardless of the concentration used. These essential oils were then used to treat seeds infected with C. gloeosporioides, evaluating the physiological and health quality of these seeds. Clove essential oil reduced the incidence of fungi in seeds inoculated with the pathogen, without affecting their physiological quality.

Influence of Leaf Wetness Duration and Temperature on Infection of Grape Leaves by Elsinoë ampelina under Controlled and Vineyard Conditions.

On susceptible varieties, indirect damage to vines infected by Elsinoë ampelina range from reduced vigor to complete defoliation while, on berries, damage ranges from reduced quality to complete yield loss. Limited knowledge about the relationship between weather conditions and infection makes anthracnose management difficult and favors routine application of fungicides. The influence of leaf wetness duration and temperature on infection of grape leaves by E. ampelina was studied under both controlled and vineyard conditions. For the controlled conditions experiments, the five youngest leaves of potted vines (Vidal) were inoculated with a conidia suspension and exposed to combinations of six leaf wetness durations (from 0 to 24 h) and six constant temperatures (from 5 to 30°C). A week after each preset infection period, the percent leaf area diseased (PLAD) was assessed. At 5°C, regardless of the leaf wetness duration, no disease developed. At 10 and at 15 to 30°C, the minimum leaf wetness durations were 4 and 6 h, respectively. Above the minimum wetness duration, at temperatures from 10 to 30°C, PLAD increased linearly, with increasing leaf wetness up to 12 h, and then at a lower rate from 12 to 24 h. The optimal temperature for infection was 25°C. Relative infection was modeled as a function of both temperature and wetness duration using a Richards model (R2 = 0.93). The predictive capacity of the model was evaluated with data collected in experimental vineyard plots exposed to natural wetness durations or artificial wetness durations created using sprinklers. In total, 264 vineyard infection events were used to validate the controlled experiments model. There was a linear relationship between the risk of infection estimated with the model and the observed severity of anthracnose (R2 = 90); however, the model underestimated disease severity. A risk chart was constructed using the model corrected for vineyard observations and three levels of risk, with light, moderate, and severe risks corresponding to ≤5, >5% to ≤25, and >25% leaf area diseased, respectively. Overall, 93.9% of 132 independent observations were correctly classified, with 100, 29.4, and 9.4% of the light, moderate, and severe risks, respectively.

Evaluation of germplasm, fungicides and biocontrol agents against anthracnose (Colletotrichum gloeosporioides) in mango (Mangifera indica) nursery

Mango anthracnose caused by Colletotrichum gloeosporioides Penz. is economically important disease of mango (Mangifera indica L.). The experiments were conducted to evaluate the germplasm and efficacy of fungicides, biocontrol agents against anthracnose in mango nursery at Punjab Agricultural University, Ludhiana, University Seed Farm, Ludhiana and M S Randhawa Fruit Research Station, Gangian, Hoshiarpur during 2017-18. Twenty five mango cultivars were screened both under laboratory conditions by using detached leaf method and under field conditions. All the cultivars had shown susceptible reaction under laboratory and field conditions with highly susceptible reaction of Malika under laboratory conditions. The fungicides (carbendazim, thiophanate methyl, azoxystrobin, propiconazole, difenoconazole, copper oxychloride, mancozeb) and two biocontrol agents (Trichoderma harzianum and Pseudomonas fluorescens) were evaluated in vitro through poisoned food technique and dual culture technique, respectively. Azoxystrobin and propiconazole were found to be highly effective in inhibiting the mycelial growth (100%) of C. gloeosporioides at 50 μg/ml and 100 μg/ml and at 100 μg/ml, respectively. All the fungicides and biocontrol agents, tested in vitro were also evaluated under field conditions for management of mango anthracnose. Azoxystrobin and propiconazole were significantly effective in reducing per cent disease index and providing disease control at Ladhowal, Ludhiana and Gangian, Hoshiarpur.

Identification of Colletotrichum Species Associated with Blueberry Anthracnose in Sichuan, China.

Anthracnose caused by Colletotrichum spp. is an important disease of blueberries and results in large economic losses for blueberry growers. Samples of anthracnose were collected from six main blueberry cultivation areas in Sichuan Province. In total, 74 Colletotrichum isolates were obtained through a single-spore purification method and identified to the species through morphological characteristics and phylogenetic analyses based on partial DNA sequences of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), internal transcribed spacer (ITS) regions, and the β-tubulin (TUB2), actin (ACT) and calmodulin (CAL) genes. Among all species, Colletotrichum fructicola was the most dominant species, with an isolation percentage of up to 66.2% in Sichuan, followed by Colletotrichum siamense (17.6%), C. kahawae (5.4%), C. karstii (5.4%), C. nymphaeae (2.7%) and C. sichuaninese (2.7%). Pathogenicity tests showed all species were able to cause typical anthracnose symptoms on blueberry leaves and stems. Colletotrichum fructicola was the predominant species with strong aggressiveness. Moreover, C. fructicola, C. kahawae, C. sichuaninese and C. nymphaeae are first reported here to cause blueberry anthracnose. This study provides a comprehensive reference for the association of different Colletotrichum spp., which may support the sustainable management of blueberry anthracnose.

Alternative seed treatment methods for plant pathogen control in sweet pepper crops

The health and physiological quality of sweet pepper seeds were assessed along with the use of alternative methods by heat therapy, plant extracts, chemical and biological products to control plant pathogens associated to the seeds. The in vitro experiments assessed the effect of the treatments applied to the seeds to control the identified plant pathogens. After treatment, the seeds were plated, incubated and assessed after seven days. An in vivo experiment was also carried out with the treatments that presented the best plant pathogen control in the seeds, which were treated and sown and after 30 days the plants were sprayed with a C. gloeosporioides suspension. The severity was assessed by a score scale specific to the pathogen system. The treatments with best plant pathogen control in the seeds were aqueous basil extract 5%, heat therapy 45° C/25 minutes, Thiophanate methyl, Quality® and B. methylotrophicus isolate, MGSS 274. In anthracnose management, the basil extract, MGSS 274 and Quality® reduced disease severity in sweet pepper plants.

In vitro and in vivo, biocontrol activity of extracts prepared from Egyptian indigenous medicinal plants for the management of anthracnose of mango fruits

Anthracnose is a very restrictive disease disturbing production, as well as postharvest property of mango fruit in Egypt. Fifteen isolates of Colletotrichum gloeosporioides were recovered from mango fruits displayed anthracnose symptoms. PCR assay was achieved in addition to morphological study confirmed that the causative agent of anthracnose in mango fruits is C. gloeosporioides. Anthracnose control of mango fruits by plant extracts was verified. Both ethanol and aqueous extracts prevented the growth of the pathogen in some degrees in vitro. Aqueous and ethanol extracts of Plantago sinaica were the strongest on fungal growth than other plants. A qualitative phytochemical investigation of plant extracts from testing plants designated saponins, flavonoids, alkaloids, tannins, and phenols. Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants. Analysis of phenolic compounds of Plantago sinaica extract using HPLC showed that ellagic acid represented the highest quantity than other compounds. Electron microscopy showed changes in the ultrastructure of the pathogen hyphae after treatment by Plantago sinaica extract.

Analysis of Colletotrichum musae populations from Brazil reveals the presence of isolates with high competitive ability and reduced sensitivity to postharvest fungicides

AbstractIn this study, the sensitivity of 218 isolates of Colletotrichum musae to imazalil and thiabendazole was evaluated, as well the fitness and competitive ability of less sensitive isolates. There was a positive correlation between the sensitivity to the two fungicides, but the isolates were more sensitive to imazalil. The estimated effective concentration of the fungicide able to inhibit mycelial growth by 50% (EC50) was used to select four isolates with the lowest and the highest values for both fungicides, which were considered as sensitive (S) and less sensitive (LS), respectively. The level of sensitivity was maintained after 10 successive transfers on fungicide‐free medium. Both fungicides were effective in controlling the disease caused by S isolates of C. musae in detached banana fruit when recommended doses were used. However, only imazalil was able to control the disease caused by LS isolates. For both fungicides, analysis of fitness‐related variables (mycelial growth, sporulation, germination, and virulence) showed no difference between the groups of S and LS isolates, but a large variation was observed within the group. The LS isolates to thiabendazole that showed a mutation (F200Y) in the β‐tubulin gene did not have fitness penalties. Our results allow a better understanding of the sensitivity and fitness of isolates of C. musae from Brazil, and demonstrate the importance of periodic monitoring to determine the frequency of LS isolates in populations, aiming at more effective management of anthracnose in banana orchards in Brazil.

Managing sweet pepper anthracnose by choice of planting season, organic soil mulch, fungicide and moderate nitrogen fertilization

Sweet pepper crops suffer major losses from anthracnose caused by Colletotrichum spp. In the present work we describe the combined effects of cultivation in the dry and rainy seasons, of nitrogen fertilization doses (0-450 Kg/ha), the use of organic (grass) and plastic soil mulches, as well as the application of a synthetic protectant fungicide (chlorothalonil) for disease control. Overall results indicate that choice of planting season is the most decisive factor for sweet pepper anthracnose management. Use of fungicide is a less decisive but still important factor, followed by choice of soil mulch substrate and nitrogen fertilization. The combined effects of all cultural practices were demonstrated: for instance, in the rainy season, fungicide alone was not as efficient as the combined use of fungicide and grass mulch. Conversely, in the dry season fungicide applications may be discarded, when organic soil mulch is adopted. The effects of the type of soil cover on the splash dispersal of Colletotrichum conidia are discussed. Management practices described here are of practical value for either organic or conventional growers.

Characterization and Fungicide Sensitivity of Colletotrichum Species Causing Strawberry Anthracnose in Eastern China.

Strawberry anthracnose caused by Colletotrichum spp. is one of the most serious diseases in the strawberry fields of China. In total, 196 isolates of Colletotrichum were obtained from leaves, stolons, and crowns of strawberry plants with anthracnose symptoms in eastern China and were characterized based on morphology, internal transcribed spacer (ITS), and β-tubulin (TUB2) gene sequences. All 196 isolates were identified as the Colletotrichum gloeosporioides species complex. In total, 62 strains were further identified at the species level by phylogenetic analyses of multilocus sequences of ITS, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), Apn2-Mat1-2 intergenic spacer and partial mating type (ApMat), calmodulin (CAL), and TUB2. Three species from the C. gloeosporioides species complex were identified: Colletotrichum siamense, C. fructicola, and C. aenigma. Isolates of C. siamense were tolerant to high temperatures, with a significantly larger colony diameter than the other two species when grown above 36°C. The inoculation of strawberry plants confirmed the pathogenicity of all three species. C. siamense isolates resulted in the highest disease severity. The in vitro sensitivities of C. siamense and C. fructicola isolates to azoxystrobin and three demethylation-inhibitor (DMI) fungicides (difenoconazole, tebuconazole, and prochloraz) were determined. Both species were sensitive to DMI fungicides but not to azoxystrobin. C. siamense isolates were more sensitive to prochloraz, while C. fructicola isolates were more sensitive to difenoconazole and tebuconazole. The present study provides valuable information for the effective management of strawberry anthracnose.

English

Common bean is among the most important legume crop for protein source in people’s diet globally and including Kenya. Anthracnose is a common disease of legumes that causes yield loss of up to 90-100%. The aim of the study is to investigate the morphological traits associated with anthracnose resistance in selected common bean genotypes in Kenya. The study was done in three varied agro-ecological zones; University of Eldoret, Bungoma and Busia. Fifteen genotypes were evaluated on field experiment to ascertain morphological traits associated with anthracnose resistance. Field experiment was done in a random complete block design. Data were collected on morphological traits and subjected to analysis of variance in SAS version 9.1. The genotypes, Ciankui, Tasha, KK15, KK8, Miezi mbili and Chelalang showed morphological traits that were significantly (P ≤ 0.05) associated with anthracnose resistance, and also with high grain yields of 1.5 to 2.0 t/ha.  Morphological traits associated with common bean anthracnose resistance included Leaf width, leaf length, length of fifth internode of the stems, bracteolate size classification and flower colour. It is recommended that management of anthracnose by use of resistant common bean genotype seeds is essential to provide increased bean yields globally and in Kenya.   Key words: Common bean, anthracnose, morphological traits, resistance.

Paralogous CYP51 Genes of Colletotrichum spp. Mediate Differential Sensitivity to Sterol Demethylation Inhibitors.

Colletotrichum spp. isolates contain two paralogous CYP51 genes that encode sterol 14-demethylase enzymes; however, their role in sensitivity to demethylation inhibitor (DMI) fungicides is yet to be determined. In this study, each of the two genes from Colletotrichum fioriniae and C. nymphaeae was able to rescue the function of CYP51 in the yeast Saccharomyces cerevisiae, demonstrating their independent function. Deletion of CYP51A led to increased sensitivity to propiconazole, diniconazole, prothioconazole, cyproconazole, epoxiconazole, flutriafol, prochloraz, and difenoconazole in C. fioriniae, and to the same fungicides and tebuconazole in C. nymphaeae, with the exception of prochloraz. Deletion of CYP51B in C. fioriniae and CYP51B in C. nymphaeae made mutants increasingly sensitive to five of nine DMI fungicides tested. The results suggest species-specific, differential binding of DMI fungicides onto the two CYP51 enzymes. Pairing DMIs with different effects on CYP51A and -B deletion mutants resulted in synergistic effects, as determined in mycelial growth inhibition experiments. Deletion mutants showed no fitness penalty in terms of mycelial growth, sporulation, and virulence. Our study elucidates the effect of CYP51A and CYP51B of Colletotrichum spp. on DMI sensitivity, suggesting that using a mixture of DMIs may improve the efficacy for anthracnose management.

Effect of Application Rate and Timing on Residual Efficacy of Pyraclostrobin in the Control of Pepper Anthracnose.

Anthracnose is a devastating disease that seriously affects pepper production worldwide. Anthracnose management is currently a major problem because of the widespread and long period of infection of this disease. Therefore, determination of the optimal fungicide application timing is important for controlling anthracnose in a timely manner . In vitro sensitivity tests showed no significant difference in the pyraclostrobin sensitivity of Colletotrichum scovillei collected from 2016 and 2017, with mean half maximal effective concentration values of 0.349 to 0.542 and 0.0475 to 0.0639 mg/liter for the inhibition of mycelial growth and spore germination, respectively. Fungicide application initiated at the full-bloom stage could significantly delay anthracnose disease onset, decrease anthracnose incidence and development (23.67 to 89.80%), and increase pepper yield by 10.7 to 29.2%. In addition, the application dosage was decreased by >50%. BF-500-3, the main metabolite of pyraclostrobin, was detected in pepper fruit and exhibited high inhibitory activity against C. scovillei. The final residues of all fungicides at different application timing were below maximum residue limits. Moreover, structural equation modeling indicated that application timing plays the most important role in anthracnose disease inhibition. The tank mixtures of pyraclostrobin with tebuconazole and fludioxonil showed more satisfactory efficacy (69.87 to 78.36%) against anthracnose than did pyraclostrobin alone under field conditions. This study is the first to determine the best fungicide application timing for anthracnose management. These results establish the basis for sustainable development of the pepper industry.

Management of postharvest anthracnose of banana using inorganic salts alone and in combination with hot water

Experiments were conducted to evaluate selected inorganic salts alone and in combination with hot water for management of banana anthracnose disease. The efficacy of hot water treatment (HWT) and either sodium bicarbonate (SBC), sodium carbonate (SC) or sodium hypochlorite (SH) applied alone and in combination to suppress anthracnose disease was investigated in both naturally infected and artificially inoculated banana fruit cv. Kolikuttu during the storage. The in vitro efficacy of the selected salts on the inhibition of colony growth of the anthracnose fungal pathogen was also tested. Postharvest treatment involving fruit dipped for 30 min in 1% (w/v) SC following HWT (50°C, 3 min) reduced the severity of anthracnose, which was statistically similar (P > 0.05) with that of homai fungicide treatment. Among the tested salts, SC recorded the significantly highest (P < 0.05) in vitro inhibition of mycelial growth of anthracnose pathogen. Integration of SC with HWT could be a commercially acceptable and economically feasible non-fungicidal approach for the postharvest management of anthracnose during the storage of banana.

Management of Strawberry Anthracnose Using Plant Essential Oils as Bio-fungicides, and Evaluation of Their Effects on Quality of Strawberry Fruit.

Colletotrichum nymphaeae is the causal agent of strawberry anthracnose, which is one of the most important disease affecting strawberry plant in Iran. This research aimed to apply the selected plant essential oils (EOs) such as Achillea millefolium, Mentha longifolia, and Ferula kuma to the management of strawberry anthracnose disease under in vitro, in vivo, and greenhouse conditions. In vitro tests indicated that all the EOs and fungicide were able to inhibit mycelial growth and conidial germination of the pathogen. Scanning electron microscopy (SEM) revealed that EOs significantly suppressed the mycelia growth and caused a change in morphology of fungal mycelia. The severity of strawberry anthracnose disease was significantly (p ≤ 0.05) reduced by all EOs under in vivo and greenhouse conditions. Results of all experiments showed that M. longifolia EO was the best EO to control C. nymphaeae. Also, EOs almost reduced weight loss and preserved firmness, ascorbic acid, total phenol, antioxidant activity (DPPH), and enzyme peroxidase activity in treated fruit. Moreover, EOs preserved the sensory quality of strawberry fruit during the storage period so that there were no significant differences between treatments (EOs) in their appearance, flavor, odor attributes, and overall evaluation compared to the control. Our results indicate that EOs are excellent bio-fungicides for the management of strawberry anthracnose.

Efficacy of fungicides for management of anthracnose (Gloeosporium psidii) in Guava

Anthracnose caused by Gloeosporium psidii (Colletotrichum psidii) is one of the major diseases causing significant losses in the production of guava. For its management, different fungicides namely; thiophanate methyl 70 WP, hexaconazole 5% EC, hexaconazole 4% + zineb 68% WP and carbendazim 12% + mancozeb 63% WP, mancozeb 75 wP and copper oxychloride 50 wP were screened against G. psidii under in vitro conditions through poisoned food technique. Carbendazim 12% + mancozeb 63% WP and thiophanate methyl 70 WP were found significantly effective by inhibiting the mycelial growth of the pathogen. All the fungicides tested in vitro were also evaluated through foliar spray for the management of disease under in vivo. Carbendazim 12% + mancozeb 63% WP and thiophanate methyl 70 WP proved to be effective for controlling anthracnose of guava under field conditions.