Multidisciplinary geochronological, isotopic, chemical, and facial studies in the Malyi Karatau Range (MK) of South Kazakhstan elucidate the Precambrian stratigraphic framework and evolution of the Ishim Middle Tianshan microcontinent (IMT) in the western Central Asian Orogenic Belt. Detrital zircon and apatite U-Pb ages for siliciclastic rocks and chemostratigraphic Sr-C isotopic data for carbonates indicate that they deposited during 800–730 Ma. The sediments are dominated by deeper marine facies in the southwest and shallow marine facies in the northeast. According to paleocurrent indicators, the main provenance located to the west of the basin. Based on the detrital zircon age spectra, the source terrane represented a Paleoproterozoic to Archean crustal block, reworked by an early Neoproterozoic c. 850–720 Ma continental arc. Erosion of Archean and Paleoproterozoic crust is also evidenced by negative εNd values for the sandstones. The petrographic and chemical compositions of the sandstones are consistent with a continental arc source; however anomalously high concentrations of chromium in some layers point to the presence of ultramafic rocks in the source terrane. The ages of metamorphic zircons indicate a high-grade metamorphic event at provenance at c. 2.0 Ga and the ages of detrital apatites suggest a reset of U-Pb isotope system in apatite at c. 1.8–1.9 Ga. In the early Neoproterozoic, the MK located between the continental arc in the west and the oceanic basin in the east and represented a fore-arc basin. The similarity of the Precambrian magmatic and metamorphic histories and sedimentary facies indicates that the IMT, the Tarim and Yangtze cratons constituted a single Precambrian Ulutau-Tarim-Yangtze Continent (UTY). Judging from the ages of continental arcs that evolved on the northern and southern sides of the UTY, it represented an independent continent at c. 950–840 Ma and was incorporated in Rodinia at c. 830–820 Ma.