Malting Quality Of Barley Research Articles

QTL mapping reveals malt barley quality improvement in two dryland environments associated with extended grain fill and seminal root traits

AbstractTo achieve malt grade and receive full price, barley (Hordeum vulgare L.) crops must meet standards for certain quality traits including percent plump and protein. Terminal drought stress reduces quality and is projected to worsen in barley cultivation areas, underscoring the need for varieties that maintain good malt production with unreliable precipitation. The stay‐green trait extends the grain fill phase between heading and maturity and has been linked to stable quality under dry conditions. However, this relationship can be inconsistent and is not well understood. To effectively leverage a longer grain fill phenotype for drought adaptation, a better grasp of its genetics and environmental interaction is needed. Stay‐green root system differences have been observed and could be at play. We performed correlation and quantitative trait locus (QTL) analysis on grain fill duration, grain quality, and seminal root traits using a recombinant inbred line (RIL) population segregating for stay‐green. Agronomic data were collected in four field trials at two distinct semiarid locations, and roots were measured in a greenhouse assay. Earlier heading and later maturity led to improved quality in both locations and more consistent quality between locations. Earlier heading had a greater influence on quality in the drier environment, while later maturity was more impactful in the less dry environment. We observed co‐locations of seminal root trait QTLs with grain fill duration and grain quality. These QTLs lay the groundwork for further investigation into root phenotypes associated with stay‐green and the deployment of these traits in breeding for drought adaptation.

Genome-wide association study on seed dormancy in barley

Abstract Seed dormancy is an important trait associated with pre-sprouting and malting quality in barley. Genome-wide association studies (GWASs) have been used to detect quantitative trait loci (QTLs) underlying complex traits in major crops. In the present study, we collected 295 barley (Hordeum vulgare L.) accessions from Australia, Europe, Canada and China. A total of 25,179 single nucleotide polymorphism (SNP)/diversity arrays technology sequence markers were used for population structure, linkage disequilibrium and GWAS analysis. Candidate genes within QTL regions were investigated, and their expression levels were analysed using RNAseq data. Five QTLs for seed dormancy were identified. One QTL was mapped on chromosome 1H, and one QTL was mapped on chromosome 4H, while three QTLs were located on chromosome 5H. This is the first report of a QTL on the short arm of chromosome 5H in barley. Molecular markers linked to the QTL can be used for marker-assisted selection in barley breeding programmes.

Emerging approaches to improve barley malt processing and quality: Ultrasound-assisted hydration and ethanol pre-treatment to drying

This work aimed to evaluate how two emerging technologies impact the malting process and the quality of barley malt: ultrasound-assisted hydration and ethanol pre-treatment to drying. All three steps of malting (hydration, germination, and drying), as well as the resulting malt's quality indicators, were evaluated. Conventional and ultrasound-assisted (25 kHz; 91 W/L, 20 °C) hydration were carried out, followed by germination, ethanol (99.5%) pre-treatment (immersion 30 min, 25 °C), and convective drying (50 °C, 1 m/s). Texture analysis demonstrated that the different stages of the malting process modify the structure and composition by increasing or decreasing the compression force in the grain. The ultrasound-assisted hydration improved the second hydration stage, where hydration resistances were greater, reducing hydration time by 38%; moreover, ultrasound accelerated the germination step with root growth 1.17 times greater. During drying, ultrasound-assisted hydration followed by ethanol pre-treatment, reduced drying time by 38.5% (barley) and 17.8% (malt). Both evaluated technologies, therefore, intensify the malting process. In addition, it was demonstrated that their use increased the alpha (9%) and beta-amylase (7%) activity in malt and modified paste and gel-forming properties of barley and malt flours. Therefore, these results suggest that the use of ultrasound improved hydration and germination processes, and ethanol pre-treatment accelerated the drying process because of improved mass transfer, both without negative impact on malt quality.

Impact of Erwinia gerundensis as a Biocontrol Agent on the Sanitary and Technological Quality of Barley Malt

This study investigates the use of a barley-associated Erwinia gerundensis strain as a biocontrol agent for the protection of malt against fungal development and mycotoxin production during the malting of barley. First, the antifungal activity of E. gerundensis was studied via simultaneous co-cultures in Yeast Malt broth and on barley kernels artificially infected with Fusarium tricinctum. Then, the effect of E. gerundensis on the fungal load and mycotoxin content of malt obtained from naturally contaminated barley was studied by applying different doses of the bacterial strain at the steeping step of the malting process. Mycotoxin concentrations were determined using the technique of liquid chromatography coupled to the mass spectrometry detector in tandem (LC-MS/MS). Also, the effect of E. gerundensis on the technological quality of the final malt was evaluated using European Brewery Convention standard methods. The bacterial strain reduced the production of enniatin (ENN) by 97.7–99.8% and 38–68% in the liquid medium and on barley kernels, respectively, along with 0–82% Fusarium tricinctum growth reduction on barley kernels. Application of E. gerundensis at the beginning of the steeping step reduced the fungal load of the final malt by 67–72% and its ENN and deoxynivalenol (DON) contents by 32–50% and 15–33%, respectively, depending on the treatment dose. Barley malts treated with E. gerundensis showed no degradation in their technological quality, thus making E. gerundensis a potentially interesting biocontrol agent for the malting and brewing industries. Further investigation should be focused on improving the bioprotective capability of the proposed biocontrol agent.

Identification of the Key Transcription Factors Regulating the Expression of the Genes Associated with Barley Malt Quality during Malting

Barley malt is produced through a malting process; it begins with steeping followed by germination and kilning, in which dramatic changes happen for a large number of physiological and biochemical traits in barley seeds. The objectives of this study were to comprehensively investigate the phenotypic changes during malting, and identify the key regulators that modulate the expression of genes associated with malt quality traits. The results showed that there was a significant positive correlation between gibberellic acid (GA) content and the activities of some hydrolytic enzymes, including α-amylases, β-amylases, and limit dextrinase (LD), and a significant negative correlation between GA and β-glucan content. Starch content had little change, but starch granules were pitted severely during malting. Weighted gene coexpression analysis (WGCNA) identified the genes associated with the greatest changes of the examined malt traits during malting. The correlation analysis and protein-protein interaction (PPI) analysis detected several key transcriptional factor (TF) regulating genes associated with malt quality. These genes and TFs regulating malting traits are potentially useful in barley breeding for malt quality improvement.

Interaction and association analysis of malting related traits in barley.

Barley is considered as a foundation of the brewing and malting industry. Varieties with superior malt quality traits are required for efficient brewing and distillation processes. Among these, the Diastatic Power (DP), wort-Viscosity (VIS), β-glucan content (BG), Malt Extract (ME) and Alpha-Amylase (AA) are controlled by several genes linked to numerous quantitative trait loci (QTL), identified for barley malting quality. One of the well-known QTL, QTL2, associated with barley malting trait present on chromosome 4H harbours a key gene, called as HvTLP8 that has been identified for influencing the barley malting quality through its interaction with β-glucan in a redox-dependent manner. In this study, we examined to develop a functional molecular marker for HvTLP8 in the selection of superior malting cultivars. We first examined the expression of HvTLP8 and HvTLP17 containing carbohydrate binding domains in barley malt and feed varieties. The higher expression of HvTLP8 prompted us to further investigate its role as a marker for malting trait. By exploring the 1000 bp downstream 3' UTR region of HvTLP8, we found single nucleotide polymorphism (SNP) in between Steptoe (feed variety) and Morex (malt variety), which was further validated by Cleaved Amplified Polymorphic Sequence (CAPS) marker assay. Analysis of 91 individuals from the Steptoe x Morex doubled haploid (DH) mapping population revealed CAPS polymorphism in HvTLP8. Highly significant (p<0.001) correlations among ME, AA and DP malting traits were observed. The correlation coefficient (r) between these traits ranged from 0.53 to 0.65. However, the polymorphism in HvTLP8 did not correlate effectively with ME, AA, and DP. Altogether, these findings will help us to further design the experiment regarding the HvTLP8 variation and its association with other desirable traits.

Spring barley malt quality in eastern Washington and northern Idaho

AbstractMalted barley (Hordeum vulgare L.) is a foundational ingredient for the brewing industry, providing functionality and flavor. Increasing demand for locally produced barley, supported by the craft brewing and malting industries, necessitates identifying environments and varieties to meet this demand. Therefore, we analyzed malt quality and quantified quality scores for adjunct (AQS) and all‐malt (AMQS) brewing for 12 barley genotypes grown at 11 locations in eastern Washington and Northern Idaho in 2017 and 2018. Genotype‐by‐environment interactions were identified for six out of nine of the malt quality traits. These included kernel plumpness, malt extract, diastatic power, α‐amylase, β‐glucan, and free amino nitrogen. Stability indices, including Eberhart and Russell's deviation from regression, simultaneous selection index, and mean of the relative performance of genotypic values were quantified. We identified genotypes that had high stability and high AQS (LCS Genie, LCS Odyssey, 12WA 120.14, 12WA 120.23, and GemCraft) or AMQS (LCS Odyssey, LCS Genie, Palmer, 11WA 107.36, 11WA 107.58). Significant correlations between rainfall and barley protein (r = −0.22, p &lt; 0.001), and between barley protein and seven out of eight of the other malt quality traits, reveal important relationships that may influence malt quality. This study may aid producers in variety selection, and provides a detailed malt quality analysis, evidence of G×E interactions, and stability measures for barley genotypes within a production region that has the potential to meet malting barley demand.

Malting quality and preharvest sprouting traits are genetically correlated in spring malting barley

Key messageMalt for craft “all-malt” brewing can have high quality, PHS resistance, and malted in normal timeframes. Canadian style adjunct malt is associated with PHS susceptibility.Expansion of malting barley production into non-traditional growing regions and erratic weather has increased the demand for preharvest sprouting (PHS) resistant, high quality malting barley cultivars. This is hindered by the relatively unknown relationships between PHS resistance and malting quality. Here we present a three-year study of malting quality and germination at different after-ripening durations post physiological maturity. Malting quality traits alpha amylase (AA) and free amino nitrogen (FAN) and germination rate at six days post PM shared a common association with a SNP in HvMKK3 on chromosome 5H in the Seed Dormancy 2 (SD2) region responsible for PHS susceptibility. Soluble protein (SP) and soluble over total protein (S/T) both shared a common association with a marker in the SD2 region. Significant genetic correlations between PHS resistance and the malting quality traits AA, FAN, SP, S/T were detected across and within HvMKK3 allele groups. High adjunct malt quality was related to PHS susceptibility. Selection for PHS resistance led to a correlated response in malting quality traits. Results strongly suggest pleiotropy of HvMKK3 on malting quality traits and that the classic “Canadian-style” malt is caused by a PHS susceptible allele of HvMKK3. PHS susceptibility appears to benefit the production of malt intended for adjunct brewing, while PHS resistance is compatible with all-malt brewing specifications. Here we present our analysis on the effect of combining complexly inherited and correlated traits with contrasting goals to inform breeding practice in malting barley, the general principles of which can be extended to other breeding programs.

Selection of favorable alleles of genes controlling flowering and senescence improves malt barley quality.

The online version contains supplementary material available at 10.1007/s11032-022-01331-7.

Dimension Reduction of Digital Image Descriptors in Neural Identification of Damaged Malting Barley Grains.

The paper covers the problem of determination of defects and contamination in malting barley grains. The analysis of the problem indicated that although several attempts have been made, there are still no effective methods of identification of the quality of barley grains, such as the use of information technology, including intelligent sensors (currently, quality assessment of grain is performed manually). The aim of the study was the construction of a reduced set of the most important graphic descriptors from machine-collected digital images, important in the process of neural evaluation of the quality of BOJOS variety malting barley. Grains were sorted into three size fractions and seed images were collected. As a large number of graphic descriptors implied difficulties in the development and operation of neural classifiers, a PCA (Principal Component Analysis) statistical method of reducing empirical data contained in the analyzed set was applied. The grain quality expressed by an optimal set of transformed descriptors was modelled using artificial neural networks (ANN). The input layer consisted of eight neurons with a linear Postsynaptic Function (PSP) and a linear activation function. The one hidden layer was composed of sigmoid neurons having a linear PSP function and a logistic activation function. One sigmoid neuron was the output of the network. The results obtained show that neural identification of digital images with application of Principal Component Analysis (PCA) combined with neural classification is an effective tool supporting the process of rapid and reliable quality assessment of BOJOS malting barley grains.

Application of Plackett-Burman Experimental Design for Investigating the Effect of Eight Phytohormones on Malt Quality Parameters

Barley is the major raw material for the malting and brewing industries. Seed germination is a fundamental process for malting and is affected by physical and chemical factors, especially plant hormones. It is well recognized that the phytohormones gibberellic acid (GA) and abscisic acid (ABA) are the primary hormones that antagonistically regulate barley germination. Other plant hormones, including auxin (IAA), ethylene (ET), brassinosteroid (BR), jasmonic acid (JA), salicylic acid (SA), cytokinins (CTKs), and strigolactone (SLs) also regulate seed germination by mediating the ABA/GA balance. In this study, the effects of eight hormones added during barley germination were investigated. An efficient design of the experimental method was employed to assess the effect of eight factors (α-amylase, β-amylase, limit dextrinase, β-glucanase, wort sugar profile, FAN, and β-glucan content) on epicotyl and root elongation. Almost all studied phytohormones played a significant role on barley epicotyl elongation. GA, ET, BA (benzylaminopurine), and zeatin (ZEA) exerted an increased effect, while ABA, SA, and BR showed a decreased effect for α-amylase, β-amylase, limit dextrinase, and β-glucanase activity. Results showed that in addition to GA and ABA, that SA, ET, BR, BA, ZEA, and JA also had significant effects on malt quality. The balance between these phytohormones is vital to the malting quality of barley. Future applications of these results could improve current recognition about the effects of phytohormones on malting and provide concrete ideas for enhancing malt quality.

On-field assessment of the environmental modulation of malting quality in barley crops

• The environmental modulation of grain attributes affecting malt extract was assessed on-field. • Malt extract was affected by protein content, grain size, pasting temperature, and d - and γ-hordeins. • The effect of protein content on malt extract was non-linear and cultivar specific. • Temperature explored during sensitivity windows within grain filling determined grain size and pasting temperature. Malt extract is the most relevant parameter describing malting quality in barley. The observed differences in malt extract from crops grown in different environments are complex to explain, and their interpretation might represent an opportunity to improve malting quality for barley crops grown under field conditions. Although the effect of some grain attributes on malt extract are overall known (e.g. protein content and grain size) other are less understood and are complex to replicate across experiments, with few evidence of how they are controlled by the environment during grain filling (e.g. hordeins and pasting properties). Four commercial malting barley cultivars were sown on eleven sowing dates across four years to explore a wide range of thermal conditions during grain filling. Contrasting nitrogen availabilities were included to promote variability in the protein content. Grain plumpness, protein content, pasting temperature, and the amount of d - and γ-hordeins showed a significant effect on malt extract. Protein content showed a negative effect on malt extract only when it was above a threshold value of ca. 10 %, although that threshold varied between cultivars. Pasting temperature presented a significant interaction with protein content. d -hordein showed a negative effect on malt extract only when the protein content was below the threshold, explaining some of the contradictory results reported in the literature. Evidence of a negative effect of γ-hordeins on malt extract was found as well. The period between pollination and physiological maturity was arbitrarily divided into thermal-time intervals, and correlation analyses were performed between mean temperature during each period and the grain attributes affecting malt extract. For all the cultivars, only two attributes were found to be modulated by the thermal environment explored by the crop during grain filling: pasting temperature was positively correlated to the mean temperature explored by the crop during the middle of grain filling, whereas grain plumpness was negatively correlated with the mean temperature during the second half of grain filling. Quantitative models reported in the present study in addition to offering an explanation of how malting quality is shaped in the field, constitute a useful tool for agro-climatic zoning for the suitability of high-quality malting barley production.

Economics of contracts in African food systems: evidence from the malt barley sector in Ethiopia

Foreign direct investment (FDI) facilitates modernization of domestic agri-food systems in emerging economies through increased use of vertical coordination. This paper sheds lights on how international brewer investments in African food systems affect smallholder market participation and value chain development. In particular, we analyze the impact of contracts among malt barley producers in Ethiopia. Using cross-sectional survey data, we employ inverse probability-weighted regression adjustment (IPWRA) and propensity score matching (PSM) techniques to analyze the economic impact of contracting. We find that contrary to popular belief, contracting has positive and significant impact on malt barley production, intensification, commercialization, quality improvement, and farm gate prices, ultimately resulting in increased net income and spillover into the productivity of other food crops.

Problems and possibilities of studying malting quality in barley using molecular genetic approaches.

About one-third of the world’s barley crop is used for malt production to meet the needs of the brewing industry. In this regard, the study of the genetic basis of malting quality traits and the breeding of malting barley varieties that are adaptive to their growing conditions are relevant throughout the world, particularly in the Russian Federation, where the cultivation and use of foreign malting varieties of barley prevails. The main parameters of malting quality (artificially germinated and dried barley grains) are malt extract, diastatic power, Kolbach index, viscosity, grain protein, wort β-glucan, free amino nitrogen, and soluble protein content. Most of these components are under the control of quantitative trait loci (QTLs) and are affected by environmental conditions, which complicates their study and precise localization. In addition, the phenotypic assessment of malting quality traits requires elaborate, expensive phenotypic analyses. Currently, there are more than 200 QTLs associated with malting parameters, which were identified using biparental mapping populations. Molecular markers are widely used both for mapping QTL loci responsible for malting quality traits and for performing marker-assisted selection (MAS), which, in combination with conventional breeding, makes it possible to create effective strategies aimed at accelerating the process of obtaining new promising genotypes. Nevertheless, the MAS of malting quality traits faces a series of difficulties, such as the low accuracy of localization of QTLs, their ineffectiveness when transferred to another genetic background, and linkage with undesirable traits, which makes it necessary to validate QTLs and the molecular markers linked to them. This review presents the results of studies that used MAS to improve the malting quality of barley, and it also considers studies that searched for associations between genotype and phenotype, carried out using GWAS (genome-wide association study) approaches based on the latest achievements of high-throughput genotyping (diversity array technology (DArT) and single-nucleotide polymorphism markers (SNPs)).

Combined effect of high temperature and drought on yield and malting quality of barley

Spring barley varieties Tolar and Marthe were grown under the standard conditions and stress conditions with a combined effect of high temperature and drought in three experiments conducted in the greenhouse of phytotron type. The results showed that growing under the stress conditions led to reduced yield, grain quality and malt quality. This effect was observed both in the individual years and on average for the entire study period. Average yield of grain declined by ca 53% and retention above 2.5 mm screen decreased by ca 28% (P = 0.001). Further, average content of proteins rose by 3.7% while starch content decreased by 5.1% (P = 0.01). In malt samples, average extract and friability were reduced by 5.8% and 15%, respectively, and average protein content increased by 3.8% (P = 0.01). The growing conditions were a dominant factor in the conducted experiments.

The Barley HvWRKY6 Transcription Factor Is Required for Resistance Against Pyrenophora teres f. teres.

Barley is an important cereal crop worldwide because of its use in the brewing and distilling industry. However, adequate supplies of quality malting barley are threatened by global climate change due to drought in some regions and excess precipitation in others, which facilitates epidemics caused by fungal pathogens. The disease net form net blotch caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres (Ptt) has emerged as a global threat to barley production and diverse populations of Ptt have shown a capacity to overcome deployed genetic resistances. The barley line CI5791 exhibits remarkably effective resistance to diverse Ptt isolates from around the world that maps to two major QTL on chromosomes 3H and 6H. To identify genes involved in this effective resistance, CI5791 seed were γ-irradiated and two mutants, designated CI5791-γ3 and CI5791-γ8, with compromised Ptt resistance were identified from an M2 population. Phenotyping of CI5791-γ3 and -γ8 × Heartland F2 populations showed three resistant to one susceptible segregation ratios and CI5791-γ3 × -γ8 F1 individuals were susceptible, thus these independent mutants are in a single allelic gene. Thirty-four homozygous mutant (susceptible) CI5791-γ3 × Heartland F2 individuals, representing 68 recombinant gametes, were genotyped via PCR genotype by sequencing. The data were used for single marker regression mapping placing the mutation on chromosome 3H within an approximate 75 cM interval encompassing the 3H CI5791 resistance QTL. Sequencing of the mutants and wild-type (WT) CI5791 genomic DNA following exome capture identified independent mutations of the HvWRKY6 transcription factor located on chromosome 3H at ∼50.7 cM, within the genetically delimited region. Post transcriptional gene silencing of HvWRKY6 in barley line CI5791 resulted in Ptt susceptibility, confirming that it functions in NFNB resistance, validating it as the gene underlying the mutant phenotypes. Allele analysis and transcript regulation of HvWRKY6 from resistant and susceptible lines revealed sequence identity and upregulation upon pathogen challenge in all genotypes analyzed, suggesting a conserved transcription factor is involved in the defense against the necrotrophic pathogen. We hypothesize that HvWRKY6 functions as a conserved signaling component of defense mechanisms that restricts Ptt growth in barley.

ECO2MALT - Assessment and improvement of the malting quality of winter barley for breeding varieties adapted to low-input crop management

La France est l’un des premiers producteurs europeens d’orge brassicole et le premier exportateur mondial de malt. Les intrants utilises sur cette culture participent aux emissions de gaz a effet de serre du secteur agricole et peuvent indirectement entrainer des effets negatifs sur la sante des consommateurs. Actuellement, la production d’orge brassicole avec un moindre recours aux intrants chimiques est limitee par le manque de varietes et d’itineraires techniques adaptes permettant de maintenir les volumes et la qualite de la production. Le cout et le temps necessaires pour realiser les analyses de qualite des grains (micromaltages) rendent l’identification des varietes adaptees aux itineraires techniques non-conventionnels delicate. Le travail realise dans le projet CasDAR Eco2Malt avait pour objectifs de concevoir et d’evaluer des ideotypes varietaux et des itineraires techniques adaptes a une production d’orge brassicole en bas niveaux d’intrants, et de proposer des methodes rapides d’estimation de la qualite brassicole des orges d’hiver 6 rangs pour accelerer la selection varietale. A travers l’experimentation, nous montrons que les varietes actuelles cultivees dans un itineraire technique avec des apports d’azote reduits (-30% -apports aux dates habituelles) et sans pesticide ne permettent pas systematiquement d’atteindre les teneurs en proteines et le rendement calibre demandes pour les orges de brasserie. Par simulation, nous montrons qu’il est indispensable de retarder les apports d’engrais, lorsque les doses sont reduites pour atteindre des performances agronomiques elevees. Nous avons identifie les caracteristiques varietales adaptees a ce mode de conduite technique. Adapter a la fois les genotypes et la strategie de fertilisation azotee permet a l’itineraire technique a bas niveau d’intrants d’atteindre des performances agronomiques similaires a celles de la conduite intensive en azote, et d’ameliorer les performances environnementales. Certains des ideotypes les plus performants en bas niveaux d’intrants obtiennent egalement des performances elevees en haut-niveaux d’intrants. Afin de limiter ces couts d’analyses du potentiel brassicole des varietes lors de la selection, un indice de selection a ete developpe et evalue, permettant de hierarchiser les caracteristiques des grains et ou du moult pour atteindre la qualite brassicole. L’interet de modeles predisant le potentiel brassicole d’une lignee a partir des analyses rapides sur grain a ete teste. Ces resultats ont pour objectif de permettre de caracteriser plus de lignees rapidement et ainsi de faciliter le developpement de lignees adaptees a differents contextes, dont les bas-intrants.

Uticaj genotipa i azotne ishrane na varijabilnost prinosa i kvaliteta zrna kod jarog pivskog ječma

Production traits and grain quality of spring malting barley as the major raw material in malt and beer production were evaluated over a period of three years (2012–2014) in the region of Požarevac, Serbia. Four cultivars of two-rowed spring barley released in the Republic of Serbia (‘Novosadski 448’, ‘Novosadski 456’, ‘Dunavac’ and ‘Jadran’) were used. The objective of this research was to assess variability in grain yield and yield components in genetically divergent cultivars of two-rowed spring barley grown at different nitrogen application rates, as dependent on precipitation and air temperature during the growing season. The results showed significant variations in grain yield, yield components and grain protein content across genotypes (G), N application rates (N) and experimental years (E). Significant interdependence was found between grain yield and yield components. Increasing the N application rate up to 135 kg/ha had a positive effect on grain yield, plant height, number of spikes per unit area, spike length and grain protein content. Number of grains per spike and 1,000-kernel weight increased significantly at N application rates up to 105 kg/ha and 75 kg/ha, respectively.

Cultural practices to improve malt barley quality in the northeast with focus on the craft sector

Demand is increasing for locally grown malt barley (Hordeum vulgare L.) in northeastern North America, driven primarily by growth in the craft beer sector. A multi-site experiment was conducted to evaluate how variety (V), seeding rate (S), and nitrogen (N) fertilizer affect malt quality in the northeast. Two barley varieties (Cerveza and Newdale), two seeding rates (200 and 400 seeds m−2), and five rates of actual applied N fertility (0, 30, 60, 90, and 120 kg ha−1) were tested at Charlottetown, PE, Canada, Ithaca, NY, US, Princeville, QC, Canada, and New Liskeard and Ottawa, ON, Canada. Basic agronomic data were collected from all environments including yield, thousand kernel weight, and hectoliter weight. Barley of suitable quality was micromalted and subjected to malt quality analysis. Both V and S resulted in small effects on malt quality, however, N had the greatest effect on most measured variables. Increased rates of N application resulted in increased yield, hectoliter weight, and thousand kernel weight but had a negative effect on most quality traits, especially with increased protein content, reduced fine extract, Kolbach index, and friability, though it increased wort β-glucans. This study shows that for most years at most sites, it is possible to achieve malt quality in the northeast; however, excessive protein and the prevalence of preharvest sprout damage are the main barriers. The results of this study have implications for increased malt barley production for the craft sector as well as potential access to commodity markets for northeastern producers.

Malting quality of seven genotypes of barley grown in Nepal.

There has been very limited work on the malting quality of barley grown in Nepal. This work used completely randomized experiment for seven barley genotypes, namely Xveola‐45, Coll#112‐114/Muktinath, Xveola‐38, Solu uwa, NB‐1003/37‐1038, NB‐1003/37‐1034, and Bonus, collected from Hill Crop Research Program (Dolakha, Nepal) to study the effect of genotypes on the chemical composition and functional properties of barley and malt. Barley was steeped for 24 hr followed by 72 hr germination in room temperature (25 ± 3°C). Germinated barley was dried (45°C/6 hr, 50°C/4 hr, 55°C/8 hr, 70°C/1 hr, 80°C/3 hr) in a cabinet drier. Multistage dried barley was then ground to pass through a 250 µm screen. Among the chemical composition, protein and reducing sugar were affected by genotype (p < .05) in barley except for β‐glucan. Functional properties, particularly bulk density, water absorption capacity, oil absorption capacity, and viscosity, were affected by genotype (p < .05) in barley, whereas except for density, all the parameters were different (p < .05) for malt. The highest diastatic power among all genotypes was recorded for solu uwa (329.25 ºDP) followed by Muktinath (271.15 ºDP). There was no significant change (p < .05) in a protein of all genotypes after malting, whereas β‐glucan and viscosity significantly decreased (p < .05) for all genotypes after malting. The remaining parameters for all genotypes were not affected (p < .05) by malting. Solu uwa had higher enzymatic activity, whereas Xveola‐38 and Muktinath were found to be better for complimentary food preparation.