Malignant diseases are considered one of the problems of our time, and cancer is defined as the abnormal growth of malignant cells. It is widely accepted as the leading cause of death. There is currently no proven cancer cure. As a result, scientists have concentrated on creating secure and efficient therapies. Research has been done on the effects of naturally occurring substances that have been extracted from living things, such fungus on cancer cells. This study sought to determine the natural products' efficacy against human cancer cell line MCF-7. After A. terreus was isolated from samples of chicken droppings, it was grown on potato and Sabouraud Dextrose Agars (SDA and PDA) with chloramphenicol media. It was then identified using the extracted genomic DNA, the internal transcribed spacer (ITS) region was amplified and sequenced. After 30 days of incubation at 27°C, natural metabolite products were also recovered from the fermentation medium using the ethyl acetate extraction technique. The effectiveness of the fungal extract against the human cancer cell line MCF-7 and the normal human cell line NHF cell was also determined after incubation for 27 hours with the natural extract. The treated human cancer cell line MCF-7 showed decrease of proliferation, whereas the normal human cell line NHF showed no effect. Significant inhibitor compared to cancer line. The IC50 values for MCF-7 cell lines and NHF normal human cell lines were 7.672 and 1431 μg/mL, respectively. In summary, MCF-7 was affected by the natural extract extracted from A. terreus, in contrast to the control. When these results were combined, they showed that the fungal extract is an effective anti-cancer treatment.