Objective: To investigate the effects of mild fatigue on the biomechanics of the lower limbs of young male volleyball players while performing single leg landing tasks. Methods: A total of ten young male volleyball players were recruited as participants in this study. After the single leg landing was performed, we compared the performance between those with and without fatigue (post- and non-fatigue, respectively). Kinematics and kinetics were collected using instruments, and related captured data were imported into OpenSim to analyze the hip, knee, and ankle joints. Results: We found that the ankle dorsiflexion angle at initial contact was significantly decreased in the post-fatigue group compared to the non-fatigue one. Meanwhile, the peak gluteus maximus force, peak gluteus medius force, peak tibialis posterior force, and peak gastrocnemius force significantly increased. There were no significant differences in the hip and knee joint flexion angles as well as the quadriceps and biceps femoris long head forces between the two groups. Conclusions: Mild fatigue can affect the performance of single leg landing, and players need to control the lower limbs by generating a higher muscle force to cope with the instability induced by fatigue. In a fatigued state, following initial contact with the ground, a decreased ankle dorsiflexion angle necessitates an increase in gastrocnemius and tibialis posterior muscle force to maintain stance during landing.