Male Samples Research Articles

Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.

Sex disparities constitute a significant issue in hepatocellular carcinoma (HCC). However, the mechanism of gender dimorphism in HCC is still not completely understood. 5-Hydroxymethylcytosine (5hmC)-Seal technology was utilised to detect the global 5hmC levels from four female and four male HCC samples. Methylation of XIST was detected by Sequenom MassARRAY methylation profiling between HCC tissues (T) and adjacent normal liver tissues (L). The role of Tet methylcytosine dioxygenase 2 (TET2) was investigated using diethylnitrosamine (DEN)-administered Tet2-/- female mice, which regulated XIST in hepatocarcinogenesis. All statistical analyses were carried out by GraphPad Prism 9.0 and SPSS version 19.0 software. The results demonstrated that the numbers of 5hmC reads in the first exon of XIST from female HCC tissues (T) were remarkably lower than that in female adjacent normal liver tissues (L). Correspondingly, DNA methylation level of XIST first exon region was significantly increased in female T than in L. By contrast, no significant change was observed in male HCC patients. Compared to L, the expression of XIST in T was also significantly downregulated. Female patients with higher XIST in HCC had a higher overall survival (OS) and more extended recurrence-free survival (RFS). Moreover, TET2 can interact with YY1 binding to the promoter region of XIST and maintain the hypomethylation state of XIST. In addition, DEN-administered Tet2-/- mice developed more tumours than controls in female mice. Our study provided that YY1 and TET2 could interact to form protein complexes binding to the promoter region of XIST, regulating the methylation level of XIST and then affecting the expression of XIST. This research will provide a new clue for studying sex disparities in hepatocarcinogenesis. XIST was significantly downregulated in HCC tissues and had gender disparity. Methylation levels in the XIST first exon were higher in female HCC tissues, but no significant change in male HCC patients. The TET2-YY1 complex regulate XIST expression in female hepatocytes. Other ways regulate XIST expression in male hepatocytes.

Influence of nano-formulations of clove bud (Syzygium aromaticum) extract on freezing ability, antioxidant capacity, caspase-3 activity, acrosome reaction and fertility of frozen rabbit semen.

The current study aimed to investigate the impact of nano-formulations of clove bud ethanolic extract (CBENF) in the extender on sperm characteristics, antioxidant capacity, oxidative biomarkers, enzymatic activity, apoptosis and fertility of post-thawed rabbit semen. Twelve mature male rabbits semen samples were pooled and cryopreserved in a Tris-egg yolk-based extender containing varying concentrations of CBENF (0, 25, 50, 75 and 100 μg/mL). After the equilibration and freezing-thawing process, CBENF (100 μg /mL) significantly enhanced progressive motility, viability and membrane integrity. Conversely, sperm abnormality was significantly reduced by CBENF supplementation. Total antioxidant capacity was increased in the post-thawed sperm medium, while nitric oxide and malondialdehyde were decreased in all CBENF concentrations. The lactic dehydrogenase and caspase-3 activities were decreased, whereas the number of live spermatozoa with an intact acrosome was increased in all CBENF concentrations. Conception rate and litter size per doe were higher in doe rabbits inseminated with semen supplemented with 100 μg CBENF/mL than un-supplemented group (76% vs. 52% and 8.4 vs. 7.7/doe), with no statistical differences. These findings suggest that supplementing rabbit extenders with 100 μg of CBENF/mL could be an effective strategy for enhancing freeze-thawing rabbit sperm attributes and fertility.

Examining the Longitudinal Connection Between Adolescent Delinquency and Risky Sexual Behaviors in a Sample of Males and Females.

Adolescence is a time period that is highlighted by widespread involvement in delinquent behaviors. The long-term consequences associated with engaging in such behaviors is not entirely understood. There is some research showing, though, that adolescents who are involved in delinquency are at-risk for also engaging in an assortment of risky sexual behaviors. Whether this association is causal or the result of selection/confounding remains unclear. The current study sought to add to the literature on this topic by examining whether measures of adolescent delinquency predict later-life involvement in risky sexual behaviors (number of sexual partners, number of one-night stands, and being diagnosed with a sexually transmitted infection) in a sample from the United States. To do so, data drawn from the National Longitudinal Study of Adolescent to Adult Health (Add Health) were analyzed (N ranging from 5018 to 5964). The results revealed statistically significant associations between adolescent delinquency and risky sexual behaviors for males and females even after controlling for levels of self-control, exposure to peer substance use, parental socialization, and neighborhood disadvantage. These results suggest that the association between delinquent involvement and risky sexual behaviors is robust and future research should begin to systematically examine the developmental nature of this association.

Spatial gene expression in the adult rat patellar tendon

Tendons are dense connective tissues with relatively few cells which makes studying the molecular profile of the tissue challenging. There is not a consensus on the spatial location of various cell types within a tendon, nor the accompanying transcriptional profile. In the present study, we used two male rat patellar tendon samples for sequencing-based spatial transcriptomics to determine the gene expression profile. We integrated our data with a mouse Achilles single cell dataset to predict the cell type composition of the patellar tendon as a function of location within the tissue. The spatial location of the predicated cell types suggested that there were two populations of tendon fibroblasts, one located in the tendon midsubstance, while the other localized with red blood cells, pericytes, and immune cells to the tendon peripheral connective tissue. Of the highest expressed spatially variable genes, there were multiple genes with known function in tendon: Col1a1, Col1a2, Dcn, Fmod, Sparc, and Comp. Further, a novel spatially regulated gene (AABR07000398.1) with no known function was identified. The spatial gene expression of tendon associated genes (Scx, Thbs4, Tnmd, Can, Bgn, Lum, Adamts2, Lox, Ppib, Col2a1, Col3a1, Col6a2) was also visualized. Both patellar tendon samples had similar expression patterns for all these genes. This dataset provides new spatial insights into gene expression in a healthy tendon.

Individuals under voluntary treatment with sexual interest in minors: what risk do they pose?

Child Sexual Abuse (CSA) and the production, use, and distribution of Child Sexual Abuse Material (CSAM) are key threats to children's mental health. From the perspective of indicated prevention, it can be assumed that some persons with a sexual interest in children commit such unreported crimes. Accordingly, the German Network kein Täter werden (meaning do not offend) has implemented a confidential treatment service for persons with a sexual interest in minors who voluntarily seek therapy, might or might not have offended but have not yet been detected or have fulfilled all legal requirements (here referred to as non-forensic individuals). However, this offer has been questioned for investing resources in a group which critics consider as low risk. The following study addresses the question of recidivism risks for CSA or viewing CSAM among non-forensic individuals. We found significantly higher rates of CSA/CSAM in our participants' history compared to a German study on a representative sample of males. Regarding CSAM, the recidivism rate of 39% was found to be 11 times higher than the expected recidivism rate based on previous publications. Regarding CSA, the recidivism rate of 14% was not significantly different from the expected rate reported for subjects with a conviction for a sexual contact offense. Among various risk instruments, only the CPORT with CASIC rating was able to predict CSA (AUC = 0.69, 95% CI = 0.55, 0.82) and CSAM (AUC = 0.63, 95% CI = 0.53, 0.73) among individuals with a history of CSAM, but with poor discrimination. We conclude that a large proportion of our sample poses a substantial risk and therefore treatment resources are well invested. However, further studies are needed to improve risk assessment among non-forensic clients.

Water-borne testosterone levels predict exploratory tendency in male poison frogs

Hormones play a fundamental role in mediating social behaviors of animals. However, it is less well understood to what extent behavioral variation between individuals can be attributed to variation in underlying hormonal profiles. The goal of the present study was to infer if individual androgen levels, and/or the modulation thereof, can explain among-individual variation in aggressiveness, boldness and exploration. We used as a model the dart-poison frog Allobates femoralis and took repeated non-invasive water-borne hormonal samples of individual males before (baseline) and after (experimental) a series of behavioral tests for assessing aggression, boldness, and exploratory tendency. Our results show that androgen levels in A. femoralis are quite stable across the reproductive season. Repeatability in wbT baseline levels was high, while time of day, age of the frog, and trial order did not show any significant impact on measured wbT levels. In general, experimental wbT levels after behavioral tests were lower compared to the respective baseline levels. However, we identified two different patterns with regard to androgen modulation in response to behavioral testing: individuals with low baseline wbT tended to have increased wbT levels after the behavioral testing, while individuals with comparatively high baseline wbT levels rather showed a decrease in hormonal levels after testing. Our results also suggest that baseline wbT levels are linked to the personality trait exploration, and that androgen modulation is linked to boldness in A. femoralis males. These results show that differences in hormonal profiles and/or hormonal modulation in response to social challenges can indeed explain among-individual differences in behavioral traits.

Explaining gender differences in crowdfunding contribution intentions

Crowdfunding represents digital fundraising channels that may enhance participation of females in project fundraising. The current study aims to analyze the gender differences in effects exerted by cognitive antecedents of financial contribution intentions (CCI) in the context of reward crowdfunding. Specifically, survey data was collected in Ghana, where crowdfunding is at its infancy, and where gender inequality is socially prevalent. Overall, 403 responses are included in the analysis. Data was analyzed using structural equation modelling (SEM), while examining associations between variables in male and female samples separately. First, when using an equality of means test, we show that females exhibit significantly higher levels of CCI, perceived risk, homophily, and prosocial orientation, as well as significantly lower levels of self-efficacy. Second, when using a between-group analysis comparing unstandardized betas, we observe that: (1) self-efficacy has stronger effects on CCI in males; (2) homophily has stronger effects on CCI in females; (3) prosocial orientation's effect on CCI does not differ between genders; (4) a stronger effect of susceptibility to social influence on CCI in females is partially evident; and surprisingly, (5) perceived risk has a stronger negative effect on CCI in males. The results, possible explanations, and implications are then discussed.

Epidemiology of genital human papillomavirus infections in sequential male sex partners of young females

ObjectivesCouple-based studies have considered human papillomavirus (HPV) transmission between current heterosexual partners (male↔female). Using data from young women and their sequential male partners, we analysed HPV transmission from upstream sexual partnerships (male 1↔female) to downstream sex partners (→male 2). MethodsAmong 502 females enrolled in the HPV Infection and Transmission among Couples through Heterosexual activity study (2005–2011, Montréal, Canada), 42 brought one male sex partner at baseline (male 1) and another during follow-up (male 2). Female genital samples, collected at six visits over 24 months, and male genital samples, collected at two visits over 4 months, were tested for 36 HPV types (n = 1512 detectable infections). We calculated observed/expected ratios with 95% CIs for type-specific HPV concordance between males 1 and 2. Using mixed-effects regression, we estimated ORs with 95% CIs for male 2 testing positive for the same HPV type as male 1. ResultsDetection of the same HPV type in males 1 and 2 occurred 2.6 (CI 1.9–3.5) times more often than chance (29 instances observed vs. 10.95 instances expected). The OR for male 2 positivity was 4.2 (CI 2.5–7.0). Adjusting for the number of times the linking female tested positive for the same HPV type attenuated the relationship between male 1 and 2 positivity, suggesting mediation. ConclusionsHigh type-specific HPV concordance between males 1 and 2 confirms HPV's transmissibility in chains of sequential sexual partnerships. HPV positivity in an upstream partnership predicted positivity in a downstream male when the linking female partner was persistently positive.

Возрастная и половая изменчивость измерительных признаков головы и лица у взрослого халхасского населения Монголии

Introduction. A number of studies have shown that the Khalka rural pastoral population of Mongolia leading a traditional lifestyle is not characterized by acceleration of development and a secular trend in somatic characteristics of the body. The purpose is to study the morphological variability of head and face features in the adult rural Khalkha-Mongolian population against the background of variability of the same features in the Chuvash group and try to catch acceleration trends based on measuring head features. Materials and methods. The data (370 men and 355 women aged 18-60) was obtained during anthropoecological expeditions in 1986-1990 in 4 Khalkha-Mongolian somons. As a comparative material, data on the Chuvash of Bashkiria were used. Regression analysis was applied. The age-related variability of normalized values of cephalometric traits was assessed using variance analysis. For the Mongolian and Chuvash populations, the coefficients of sexual dimorphism of individual features of the face and head (according to V. Deryabin`s formula), as well as the Mahalanobis distance between female and male samples were determined. Results and discussion. For the studied characteristics (head length and breadth, the minimal forehead breadth and facial breadth, face height, facial and head indexes) in men, no reliable links were found between the variability of these traits and age. Variance analysis of normalized values of cephalometric signs revealed no differences in either the male or female Mongolian sample. The Mahalanobis distances calculated from the complex of head and face signs between the female and male Mongolian subsamples are noticeably smaller than the corresponding values obtained for the Chuvash group. Conclusion. Comparative studies of the age dynamics of a number of measuring features of the head for about one and a half generations of the twentieth century in Khalkha-Mongols (adapted groups) and Chuvash (populations with impaired adaptation due to changes in socio-economic environmental factors) showed weakly pronounced morphological changes in the features on the head and face in the studied population groups. There were no significant age differences in the complex of features of the face and head in the Khalkhas populations. Data on sexual dimorphism of facial and head features in Mongols and Chuvash have been introduced into scholarly discourse, and in the Mongolian population the values of indicators of sexual dimorphism of cephalometric signs are less than in Chuvash sample. @ 2023. This work is licensed under a CC BY 4.0 license

Население золотоордынского времени Восточно-Причерноморского региона по данным остеометрии (по материалам раскопок могильника Натухаевское 5)

Introduction. Osteometric data of adult individuals of the anthropological series from the burial ground of the Golden Horde time "Natukhaevskoe 5" are presented for the first time in this work. Based on the standard measurements, patterns of the skeletal constitution of the buried are discussed. As part of this study, we tested the hypothesis about the difference in the morphological appearance of two samples, combined according to the type of funeral rite - stone boxes and burial grounds. Materials and methods. The anthropological collection consists of the remains of 124 adult individuals, including 67 males and 57 females. Measurements of the postcranial skeleton were carried out according to the program of R. Martin (in version by V.P. Alekseev) according to 55 features. The degree of homogeneity of the male and female samples was determined using an intragroup analysis with the method of principal components for 10 features. The Mann-Whitney test was used to assess the statistical significance of differences. Results. The male population was rather tall with a shortened robust humerus and an elongated forearm bones, the lower limbs were long and massive. Among the female group, there is also a tendency to lengthen the lower limbs, relative to the upper ones, mainly due to the lower leg. The conducted intragroup analysis did not reveal statistically significant differences between the two groups, united according to the type of funeral rite, neither among men nor among women. However, a large range of linear size variations was found among men buried in stone boxes. Compared to them, the sample from the ground pit is more homogeneous. A different picture is observed in the female sample, where higher morphological diversity can be traced among those buried in the pits. @ 2023. This work is licensed under a CC BY 4.0 license

Detection of anabolic agents including selective androgen receptor modulators in samples outside of sport.

Selective androgen receptor modulators (SARMs) are prohibited by the World Anti-Doping Agency (WADA) since 2008. Similarly, to anabolic androgenic steroids (AAS), SARMs are detrimental to health not only in athletes but also in the general population. However, studies of the occurrence of SARMs outside of sport are scarce. Swedish healthcare samples from the Drugs of Abuse Laboratory at Karolinska were analyzed using WADA-accredited screening methods at the Doping Control Laboratory in Stockholm to estimate the frequency of SARM use outside of the WADA laboratories. Twenty (4%) of the male urine samples (n = 542) were positive for SARMs, whereas none of the analyzed female samples (n = 100) contained any SARMs. The top three SARMs found were LGD-4033 followed by RAD140 and ostarine. Two or more SARMs were found in >50% of the SARM-positive samples. AASs were identified in 40% of samples containing SARMs. A difference between genders was observed where 34% male and 7% female samples contained AAS. Many samples displayed testosterone/epitestosterone values indicative of testosterone intake, without presence of other AAS, and hence, there is a risk that these samples are being falsely reported as negative. Our results indicate that SARM use might be a concern outside of sport. Subsequently, in addition to AAS, the healthcare system should also be informed about SARM abuse and the associated adverse side effects.

Effect of Inhaling of Perfume on the Blood of Human Healthy Male Using Electrochemical Method by Cyclic Voltammetry

All people in the world were used perfume in different manufactures and marker, in this study, we wanted to be found the effect of continues using of perfume and still inhaling these compound on the human healthy by electrochemical analysis using cyclic voltammetry. In the scientific knowledge, the inhaling of perfume by breathed and then reach to the blood stream through lungs and pulmonary alveoli passing to the blood vessels. The study focused on the oxidation – reduction peaks current of the cyclic voltammogram which can be found from the perfume in healthy blood of male samples. It was found from the results that many oxidation peaks current appeared in the cyclic voltammogram in the blood medium with presence of the perfume, also, in different concentration, and different temperatures were studied, so all perfume compound were effected on the blood components of the male. The oxidative effect of the perfume on blood medium which may be caused the damage of blood components through the continuance of using it and appeared a risk cases of different diseases. the advices for correct using of the perfume in a little amount and in limited using every day, also avoid using the perfume to the patients with high fever.

The Effect of Home Care Poverty on the Activities of Daily Living among Older Adults in China: A Propensity Score Matching Study.

In the context of home care being valued by the Chinese government and the increasing number of disabled older adults, it is of great significance to explore the effect of home care poverty of the older adults on their ability to perform activities of daily living. A research sample of 2583 older adults from the Chinese Longitudinal Healthy Longevity Survey data in 2018 was adopted. There were 422 participants in the treatment group and 2161 participants in the control group. According to the framework of counterfactual analysis, the kernel matching method was used to match the treatment and control groups to calculate the values of average treatment effects on treated (ATT). The results of the kernel matching method showed that the factual ADL score of the treatment group was 6.886, the counterfactual ADL score of the control group was 8.520, and the ATT value was -1.634 (P<0.05). There were gender and urban-rural differences in the relationship between home care poverty and activities of daily living among older people. In gender samples, there was a significant correlation between the two, and the absolute value of ATT in male samples was higher than that in female samples (P<0.05). In the rural samples, there was a significant correlation between the two variables (P<0.05). But in the urban samples, there was no significant correlation between the two variables. Home care poverty could significantly reduce the ability to perform activities of daily living among older adults in China. Based on the conclusions, the study puts forward several suggestions to solve the home care poverty for the older adults in China.

FAT1 inhibits AML autophagy and proliferation via downregulating ATG4B expression

BackgroundEmerging studies have shown that FAT atypical cadherin 1 (FAT1) and autophagy separately inhibits and promotes acute myeloid leukemia (AML) proliferation. However, it is unknown whether FAT1 were associated with autophagy in regulating AML proliferation. MethodsAML cell lines, 6-week-old male nude mice and AML patient samples were used in this study. qPCR/Western blot and cell viability/3H-TdR incorporation assays were separately used to detect mRNA/protein levels and cell activity/proliferation. Luciferase reporter assay was used to examine gene promoter activity. Co-IP analysis was used to detect the binding of proteins. ResultsIn this study, we for the first time demonstrated that FAT1 inhibited AML proliferation by decreasing AML autophagy level. Moreover, FAT1 weakened AML autophagy level via decreasing autophagy related 4B (ATG4B) expression. Mechanistically, we found that FAT1 reduced the phosphorylated and intranuclear SMAD family member 2/3 (smad2/3) protein levels, thus decreasing the activity of ATG4B gene promoter. Furthermore, we found that FAT1 competitively bound to TGF-βR II which decreased the binding of TGF-βR II to TGF-βR I and the subsequent phosphorylation of TGF-βR I, thus reducing the phosphorylation and intranuclear smad2/3. The experiments in nude mice showed that knockdown of FAT1 promoted AML autophagy and proliferation in vivo. ConclusionsCollectively, these results revealed that FAT1 downregulates ATG4B expression via inhibiting TGFβ-smad2/3 signaling activity, thus decreasing the autophagy level and proliferation activity of AML cells. General significanceOur study suggested that the “FAT1-TGFβ-smad2/3-ATG4B-autophagy” pathway may be a novel target for developing new targeted drugs to AML treatment.

Comparing the Integration of Syndromic versus Etiological Management of Sexually Transmitted Infections Into HIV Pre-Exposure Prophylaxis Services for Adolescent Girls and Young Women, in South Africa

South Africa has a high incidence of human immunodeficiency virus and sexually transmitted infections (STIs), particularly among adolescent girls and young women. National and global guidelines recommend varied strategies for integrating STI and pre-exposure prophylaxis (PrEP) services. PurposeThis paper describes the implementation of a syndromic compared to an etiological approach to STI integration within PrEP services in South Africa. MethodsWe analysed program data from eight fixed and four mobile clinics to describe a cascade of STI care and integration of syndromic management among clients accessing PrEP services. Diagnostic testing was conducted in a subset of clients to determine the prevalence of STIs and estimate the burden of disease missed using a syndromic approach. ResultsBetween December 2018 and December 2021, 22,505 clients sought services and a high proportion (92.9%) was screened for STI symptoms. Of these, 9% of females and 3% of males had symptoms and 89.5% had recorded treatment. In a subset of PrEP clients (406 females, 70 males) screened through laboratory testing, chlamydia was identified in 25.7% of female and 20.0% of male samples, gonorrhea in 14.1% of female and 18.6% of male samples, and syphilis in 2.3% of female and 1.4% of male samples. Highest prevalence was found among females aged 18–20 years. DiscussionSyndromic STI screening and management can be integrated into routine PrEP service delivery and can identify symptomatic STIs, but misses asymptomatic infections. PrEP clients have a high prevalence of treatable STIs. Etiologic approaches can identify more infections than syndromic screening, but cheap point-of-care tests are needed.

Mind mapping in recalling and retrieving core contents in anesthesia technology students

BACKGROUND: Human memory represents the ability to recall and retrieve information. Learning methods have a great impact on remembering and retrieving material. This study was conducted to investigate the effect of the mind map learning method on the amount of recall and retrieval of the core contents of anesthesia technology students. MATERIALS AND METHODS: In this quasi-experimental study, 55 undergraduate students of anesthesia technology were included in the study by census method. In both groups, teaching was done in the same way (lecture method) by a single professor. The intervention group was randomly divided into six groups. After training to work with a mind map in this group, the teaching of lessons started with the lecture method, and then the mind map was drawn by the group members. In the control group, the students followed the lecture method and note-taking. Finally, three tests were taken. Test 1 (before the start of the training), test 2 (immediately after the training (scheduled test)), and test 3 (after 2 weeks (surprise test)). RESULTS: The data obtained from the test scores were analyzed by paired t-test, independent t-test, and analysis of covariance. The analysis showed that the mind map method significantly improved the short-term (P &lt; 0.001) and long-term memory of anesthesia technology students (P &lt; 0.001) compared to the traditional method. Examining the effect of gender on long-term memory showed that in the intervention group, female samples had an average score of 1 more than male samples (P = 0.044). DISCUSSION: It seems that mind mapping improves short-term and long-term memory in anesthesia technology students who play a critical role in saving the lives of patients and this issue improves the quality of services in the field of health.

58 Examining the Link Between Self-Reported Anxiety and Aggressive Behaviors

Objective:It is well known that there are differences between men and women in anxiety and aggression. Moreover, prior research has shown an association between anxiety and aggression but the strength of these associations in males and females has not been well characterized, and it remains unclear whether such associations are driven by comorbid disorders such as posttraumatic stress disorder (PTSD) or substance abuse. Therefore, we examined these associations in a large sample of males and females, and statistically controlled for the aforementioned potential confounding variables.Participants and Methods:A total of 13,313 adults completed the survey on Amazon Mechanical Turk between April 2020 and April 2021, including 5,598 females (Mage=36.4, SD=11.9) and 7,654 males (Mage=37.81, SD=12.7). Aggression was measured using the Buss Perry Aggression Questionnaire (BPAQ), while the Generalized Anxiety Disorders (GAD-7) scale was used to gauge anxiety levels. PTSD was assessed with the PC-PTSD scale, and alcohol misuse was assessed with the Alcohol Use Disorders Inventory (AUDIT). Data were analyzed with zero-order correlations and linear regression to control for the effects of PTSD and alcohol misuse. Lastly, we used a Fisher r-to-z transformation to compare the correlations between males and females for both physical and verbal aggression with anxiety.Results:Higher aggression (i.e., BPAQ) was correlated with greater anxiety (i.e., GAD; r(13213)=0.482, p&lt;0.0001)). This association between anxiety and aggression held even when other potential confounders were controlled, such as PTSD (p&lt;0.0001), and alcohol misuse, p&lt;0.0001. Additionally, the correlation between anxiety and physical aggression was significantly stronger in males than females (z=5.02, p&lt;0.0001), a pattern that was also true for the association between anxiety and verbal aggression (z=4.13, p&lt;0.0001).Conclusions:Our findings suggest that there is a linear relationship between the severity of anxiety and the severity of both verbal and physical aggression, that these associations tend to be stronger among males, and are not accounted for by associated conditions such as PTSD or alcohol misuse. This data augments existing research on the factors that contribute to aggression and further suggest that anxious feelings are more directly associated with aggression in males. These findings raise the possibility that interventions that target anxiety may prove helpful in reducing aggressive behavior among males. It may be fruitful for future work to identify neural systems that are associated with both anxiety and aggression and which are also modulated by sex. While measures of neurochemistry were not collected here, prior research has suggested that there are sex differences in brain systems that rely on serotonergic neurotransmission and arginine vasopressin, which could provide a target for future work.

1 Psychometric Characteristics of the Grenada Learning and Memory Scale: An Innovative Tool for Preschool Memory Assessment in Resource-Limited Regions

Objective:Neuropsychological assessment of preschool children is essential for early detection of delays and referral for intervention prior to school entry. This is especially relevant in low-and middle-income countries (LMICs), which are disproportionately impacted by micronutrient deficiencies and teratogenic exposures. There are limited options for assessment of preschool learning and memory, developed and validated in resource-limited regions. The Grenada Learning and Memory Scale (GLAMS) was created for use in the Caribbean using an indigenous “ground-up” approach, with feedback from regional stakeholders at various stages of development. The GLAMS contains two subtests - a verbal list-learning task, which imagines a trip to the shop to buy culturally familiar items, and a face-name associative learning task using locally-drawn faces of Caribbean children. There are two versions: a 4-item version for 3-year-olds and a 6-item version for 4 and 5-year-olds. Here we present descriptive data and psychometric features for the GLAMS from an initial preschool sample.Participants and Methods:Participants were recruited from a social-emotional intervention study (SGU IRB#14099) in Grenada between 2019-2021. Children were between 36 and 72 months of age, primarily English-speaking, and had no known history of neurodevelopmental disorders. Trained Early Childhood Assessors administered the GLAMS and NEPSY-II in public preschools and homes across Grenada. Exploratory descriptive statistics characterized participant sociodemographics and test score distributions. Spearman correlations, MannWhitney U, and Kruskal-Wallis tests examined the impact of sociodemographics on test scores. Internal reliability was assessed with coefficient alpha. NEPSY-II subtests were used to assess convergent validity, with the prediction that the highest correlations would be observed for NEPSY-II Sentence Repetition. Test engagement (as reflected by “zero-learning”, “some learning”, and “positive learning curves”) was assessed across each age bracket (in 6-month increments). We assessed and summarized barriers to engagement qualitatively.Results:The sample consisted of 304 children (152 males,152 females). Participants were predominantly Afro-Caribbean and Indo-Caribbean. Parent education and household income (Mdn=$370-740 USD per month) were consistent with the general population. GLAMS internal consistency was reliable (a=0.713). There were age effects on list-learning (rs=0.51; p&lt;0.001), list recall (rs=0.51; p&lt;0.001), face-name learning (rs=0.30;p&lt;0.001), and face-name recall (rs=0.25; p&lt;0.001). There were gender effects on list-learning (p=0.02) and list recall (p=0.01) but not face-name learning or recall. All GLAMS subtests were correlated with NEPSY Sentence Repetition (rs=0.22-0.34; p&lt;0.001). There was sufficient sampling of males and females across all 6 age brackets. As age increased, a higher proportion of children showed a positive learning curve (and fewer “zero-scores”) on verbal learning (X2 =30.88, p&lt;0.001) and face-name learning (X2=22.19, p=0.014), demonstrating increased task engagement as children mature. There were various qualitative observations of why children showed “zero-scores”, ranging from environmental distractions to anxiety and inattention.Conclusions:As far as we know, the GLAMS is the first preschool measure of learning and memory developed indigenously from within the Caribbean. It shows reliable internal consistency, expected age and gender effects and convergent validity. These initial results are encouraging and support continued efforts to establish test-retest and inter-rater reliability. Plans include validation in clinical samples, scale-up to other Caribbean countries, and eventual adaptation across global LMICs.

Disordered eating in transgender and gender non-conforming youth: A comparison to community-based and clinical samples.

This study investigates eating pathology in transgender and gender non-conforming (TGNC) youth compared to a community-based sample and individuals with eating disorders (EDs). Participants (ages 13-21years) included TGNC youth from a paediatric gender clinic (N=97), a demographically matched community-based sample of cisgender males (N=97) and cisgender females (N=97), and treatment-seeking patients with EDs (N=112). The Eating Disorder Examination Questionnaire (EDE-Q) was used to assess ED cognitions and behaviours. Transgender and gender non-conforming participants reported significantly higher EDE-Q global scores compared to the cisgender samples, but significantly lower than the ED sample. Transgender and gender non-conforming individuals reported a higher likelihood of objective binge episodes (OBEs) than the cisgender groups, albeit lower than youth with EDs. A substantial proportion of TGNC participants scored in elevated ranges on the EDE-Q global score (35%≥score of 3, 17%≥score of 4), significantly higher than cisgender males (0%≥score of 3, 0%≥score of 4) and females (9%≥score of 3, 3%≥score of 4). Findings indicate that TGNC youth exhibit increased ED cognitions and OBEs compared to cisgender samples, highlighting the need for screening and addressing ED symptoms in this population.

Biomarkers in Body Fluids as Indicators of Skeletal Maturity: A Systematic Review and Meta-analysis.

This review aimed to critically appraise the evidence for biomarkers in blood serum, gingival crevicular fluid (GCF), saliva, and urine in comparison with standard radiographic indices for skeletal maturation assessment. A thorough literature search in multiple databases was conducted for biomarkers in body fluids for skeletal maturation assessed with cervical vertebrae in lateral cephalograms or on hand-wrist radiographs. Different combinations including free text, MeSH terms, and Boolean operators were used. Two researchers used strict inclusion and exclusion criteria to screen title, abstract, and full text, and used the Quality Assessment of Diagnostic Accuracy Studies (QUADAS)-2 instrument for risk of bias assessment of individual studies. Meta-analysis was performed on eligible studies using RevMan 5 software. A total of 344 articles were screened, of which 33 met the inclusion criteria and quality assessment. The skeletal maturity indicators included insulin-like growth factors (IGF-1), alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), dehydroepiandrosterone sulfate (DHEAS), vitamin D binding protein (DBP), parathormone-related protein (PTHrP), osteocalcin, metalloproteins, and serotransferrin (TF) along with different metabolites. At puberty, a significant rise was seen in IGF-1, DBP, ALP, osteocalcin, TF, and BALP. However, the serum DHEAS and PTHrP increased from pre-pubertal to post-pubertal stages. Due to the data heterogeneity, a meta-analysis could be performed on seven studies in total on IGF-1 in serum and blood. Of these, five were included for data in males and six in females, and four studies on IGF-1 in serum and blood. A significant difference in IGF-1 levels was seen between stages of peak pubertal growth spurt (CS3 and CS4) and decelerating pubertal growth (CS5) compared with growth initiation stage (CS2). Pubertal growth spurts were correlated with peak serum IGF-1 and BALP in both sexes individually. Peak ALP levels in GCF were correlated with the pubertal spurt in a combined sample of males and females. Standard biofluid collection protocols and homogeneity in sampling and methodology are strongly recommended for future research.