Male Body Weight Research Articles

Growth Differences of Growth Hormone Transgenic Female and Male Channel Catfish, Ictalurus punctatus, Grown in Earthen Ponds to Sexual Maturation.

This study compared growth performance between female and male transgenic channel catfish, Ictalurus punctatus, containing channel catfish growth hormone full-length cDNA driven by the ocean pout antifreeze protein promoter, opAFP-ccGH, the rainbow trout metallothionein promoter, rtMT-ccGH, or both constructs, and their non-transgenic siblings in earthen ponds at 16 and 48months of age. Body weight between the transgenic and their non-transgenicsiblings differed (P < 0.001) at all ages. Transgenic F2 opAFP-ccGH grew 1.51- to 2.58-, F2 rtMT-ccGH grew 1.44- to 2.99- and F1fish transgenic for both constructs grew 1.36- to 2.92- fold larger than their non-transgenicsibling controls, depending upon age and sex. Body weight of the transgenic GH males was significantly higher than those of the transgenic GH females at 16months of age (P < 0.001). However, body weight of the transgenic GH females was significantly higher (P < 0.001) compared with those of the transgenic GH males at 48months of age, but not for the double transgenics (P > 0.05). In the case of non-transgenic GH siblings, males were larger than females at both 16 and 48months of age (P < 0.001). Sexually dimorphic responses to GH transgenes were the opposite after sexual maturation. When critically low dissolved oxygen levels were encountered, survival of transgenic male and female opAFP-ccGH channel catfish was lower than that of controls (P = 0.004), as well as rtMT-ccGH females (P = 0.11), which is not surprising since the largest fish are most likely to succumb during an oxygen depletion.

Role of myeloid cell leptin signaling in the regulation of glucose metabolism

Although innate immunity is linked to metabolic health, the effect of leptin signaling in cells from the innate immune system on glucose homeostasis has not been thoroughly investigated. We generated two mouse models using Cre-lox methodology to determine the effect of myeloid cell-specific leptin receptor (Lepr) reconstitution and Lepr knockdown on in vivo glucose metabolism. Male mice with myeloid cell-specific Lepr reconstitution (Lyz2Cre+LeprloxTB/loxTB) had better glycemic control as they aged compared to male mice with whole-body transcriptional blockade of Lepr (Lyz2Cre−LeprloxTB/loxTB). In contrast, Lyz2Cre+LeprloxTB/loxTB females only had a trend for diminished hyperglycemia after a prolonged fast. During glucose tolerance tests, Lyz2Cre+LeprloxTB/loxTB males had a mildly improved plasma glucose profile compared to Cre− controls while Lyz2Cre+LeprloxTB/loxTB females had a similar glucose excursion to their Cre− controls. Myeloid cell-specific Lepr knockdown (Lyz2Cre+Leprflox/flox) did not significantly alter body weight, blood glucose, insulin sensitivity, or glucose tolerance in males or females. Expression of the cytokine interleukin 10 (anti-inflammatory) tended to be higher in adipose tissue of male Lyz2Cre+LeprloxTB/loxTB mice (p = 0.0774) while interleukin 6 (pro-inflammatory) was lower in male Lyz2Cre+Leprflox/flox mice (p < 0.05) vs. their respective controls. In conclusion, reconstitution of Lepr in cells of myeloid lineage has beneficial effects on glucose metabolism in male mice.

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish.

Overexpression of growth hormone (GH) in gh-transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. It is hypothesized that microRNAs are directly involved in the regulation of fertility potential during spermatogenesis. The primary aim of our study was to verify whether gh overexpression disturbs the sperm miRNA profile and influences the sperm quality in transgenic zebrafish. We report a significant increase in body weight of gh-transgenic males along with associated reduced sperm motility and other kinetic parameters in comparison to the non-transgenic group. MicroRNA transcriptome sequencing of gh-transgenic zebrafish sperms revealed expressions of 186 miRNAs, among which six miRNA were up-regulated (miR-146b, miR-200a-5p, miR-146a, miR-726, miR-184, and miR-738) and sixteen were down-regulated (miR-19d-3p, miR-126a-5p, miR-126b-5p, miR-22a-5p, miR-16c-5p, miR-20a-5p, miR-126b-3p, miR-107a-3p, miR-93, miR-2189, miR-202–5p, miR-221–3p, miR-125a, miR-125b-5p, miR-126a-3p, and miR-30c-5p) in comparison to non-transgenic zebrafish. Some of the dysregulated miRNAs were previously reported to be related to abnormalities in sperm quality and reduced reproduction ability in other species. In this study, an average of 134 differentially expressed miRNAs-targeted genes were predicted using the in silico approach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that the genes of affected pathways were primarily related to spermatogenesis, sperm motility, and cell apoptosis. Our results suggested that excess GH caused a detrimental effect on sperm microRNAome, consequently reducing the sperm quality and reproductive potential of zebrafish males.

Skin biomechanical properties and leg volume in aging healthy adults.

In adults ageing is accompanied by changes in limb volume and skin biomechanical properties. To explore the relationship between body size, aging, skin biomechanics, and leg volume, VLeg and to define predictive equations linking leg volume with these properties. Sixty-two healthy adults (Age 18-80 years, M:F 45:55) were recruited. Anthropomorphic measures were recorded along with VLeg (via circumferential method) and skin tone, stiffness, and elastance (via tonometry). Regression analysis was performed to define relationship between the measured parameters. In healthy adults bilateral VLeg were the same regardless of leg or sex, 5791 ± 1363 for females and 6151 ± 1203 mls for males. VLeg was positively correlated to body weight, where VLeg (mls)=1058 + 69 × Wt (kg) for females and VLeg (mls)= 539 + 65 × Wt (Kg) of body weight for males. Skin surface biomechanical properties varied with sex, leg volume, and location on the leg with the malleolus exhibiting the stiffest surface. The study shows that anthropometric measures change with sex and leg size are multifactorial and body weight, sex, and skin condition as important determinant factors of leg volume.

Parental exposure to sulfamethazine and nanoplastics alters the gut microbial communities in the offspring of marine madaka (Oryzias melastigma)

The individual and combined toxicity of antibiotics and nanoplastics in marine organisms has received increasing attention. However, many studies have been mostly focused on the impacts on the directly exposed generation (F0). In this study, intergenerational effects of sulfamethazine (SMZ) and nanoplastic fragments (polystyrene, PS) on the growth and the gut microbiota of marine medaka (Oryzias melastigma) were investigated. The results showed that parental exposure to dietary SMZ (4.62 mg/g) alone and PS (3.45 mg/g) alone for 30 days decreased the body weight (by 13.41% and 34.33%, respectively) and altered the composition of gut microbiota in F1 males (two months after hatching). Interestingly, parental exposure to the mixture of SMZ and PS caused a more modest decrease in the body weight of F1 males than the PS alone (15.60% vs 34.33%). The hepatic igf1 level and the relative abundance of the host energy metabolism related phylum Bacteroidetes for the SMZ + PS group were significantly higher than those for the PS group (igf1, increased by 97.1%; Bacteroidetes, 2.876% vs 0.375%), suggesting that the parentally derived mixture of SMZ and PS might influence the first microbial colonization of gut in a different way to the PS alone. This study contributes to a better understanding of the long-term risk of antibiotics and nanoplastics to marine organisms.

Maternal Chronodisruption Throughout Pregnancy Impairs Glucose Homeostasis and Adipose Tissue Physiology in the Male Rat Offspring

Compelling evidence in rats support the idea that gestational chronodisruption induces major changes in maternal circadian rhythms and fetal development and that these changes impact adult life at many physiological levels. Using a model of chronic photoperiod shifting throughout gestation (CPS), in which pregnant female rats (Sprague–Dawley strain; n = 16 per group) were exposed to lighting schedule manipulation every 3–4 days reversing the photoperiod completely or light/dark photoperiod (12/12; LD), we explored in the adult rat male offspring body weight gain, glucose homeostasis, adipose tissue content, adipose tissue response to norepinephrine (NE), and adipose tissue proteomic in the basal condition with standard diet (SD) and in response to high-fat diet (HFD). In adult CPS male (100–200 days old; n = 8 per group), we found increasing body weight, under SD and adiposity. Also, we found an increased response to intraperitoneal glucose (IGTT). After 12 weeks of HFD, white adipose tissue depots in CPS offspring were increased further, and higher IGTT and lower intraperitoneal insulin tolerance response were found, despite the lack of changes in food intake. In in vitro experiments, we observed that adipose tissue (WAT and BAT) glycerol response to NE from CPS offspring was decreased, and it was completely abolished by HFD. At the proteomic level, in CPS adipose tissue, 275 proteins displayed differential expression, compared with LD animals fed with a standard diet. Interestingly, CPS offspring and LD fed with HFD showed 20 proteins in common (2 upregulated and 18 downregulated). Based on these common proteins, the IPA analysis found that two functional pathways were significantly altered by CPS: network 1 (AKT/ERK) and network 2 (TNF/IL4; data are available via ProteomeXchange with identifier PXD026315). The present data show that gestational chronodisruption induced deleterious effects in adipose tissue recruitment and function, supporting the idea that adipose tissue function was programmed in utero by gestational chronodisruption, inducing deficient metabolic responses that persist into adulthood.

Sire evaluation and effect of various factors on growth performance in males of Tharparkar and Karan fries cattle

The current study was undertaken with the objective of sire evaluation as well as studying the effect of genetic and non-genetic factors on growth performance in males of Tharparkar and Karan Fries Cattle. Data on body weights of Tharparkar and Karan Fries males born during the period 1994-2012 at ICAR-NDRI, Karnal, were utilized. The overall least squares means of birth weight and weights at one-month interval up to 12 months of age were 26.92±0.24, 35.55±0.22, 45.04±0.28, 57.56±0.40, 70.02±0.51, 82.62±0.66, 95.18±0.80, 105.11±1.55, 108.19±1.15, 114.20±1.15, 120.11±1.09, 130.35±1.11 and 136.91±4.13 kg respectively, in KF males. In TP males, least square means for birth and one-month were 21.34±0.77 and 25.63±0.67 kg, respectively. Effect of month and period of birth was highly significant on all traits except 1 month and 12-months for the former effect. The effects of genetic group and parity were mostly significant on body weights in KF males. Regression on birth weight was highly significant for all the weight traits. Effect of sire and birth-month was highly significant on all traits except one-month weight in KF. In Tharparkar, effect of season was highly significant on birth weight. Growth curves were linear, and deviations of average body weekly weight showed more divergence with increasing age. Growth rates from birth to three-months age in KF and TP males were 320 g/day and 226 g/day. Effect of non-genetic factors indicated the need of further improvement in feeding and management practices for augmenting growth, i.e. body weights especially in calves born to primiparous dams.

Maternal obesity (MO) programs morphological changes in aged rat offspring small intestine in a sex dependent manner: Effects of maternal resveratrol supplementation

Maternal obesity (MO) leads to offspring metabolic problems. The mechanisms involved are multifactorial. The small intestine plays an important role in the absorption of nutrients and is modified as we age. Few studies have explored MO programming effects on offspring (F1) small intestine morphology. The aim of this study was to investigate MO effects on old adult F1 intestinal morphology, and whether any F1 intestinal changes due to MO were modified by maternal resveratrol supplementation. From weaning throughout pregnancy and lactation, female Wistar rats (F0) ate standard chow (controls, C: 5%-fat) or high-fat diet (MO: 25%-fat). One month before mating at postnatal day (PND) 120 through lactation half of each group received 20 mg/kg/day of resveratrol orally (Cres or MOres). After weaning F1 were fed with chow diet until the end of the study at PND 650. Body weight, percent of fat, glucose, cholesterol and triglyceride serum concentrations were determined. F1 small intestinal samples were collected for histological analysis. Male F1 body weight was higher in MO and MOres compared with C and Cres. Female F1 body weight and percent of fat was higher in MO than C and MOres. Triglyceride concentrations were higher in MO and MOres male F1 compared with C and Cres. There were no differences among groups in female triglyceride concentrations. Male F1 duodenal villus height was smaller in MO compared with MOres. Female F1 duodenal and jejunal crypt depth was smaller in MO compared with C and was greater compared with MOres. Female F1 villus height in jejunum was greater in MO compared with MOres. In conclusion, exposure to the developmental challenge of MO changed the aged F1 intestinal morphological and metabolic profiles. Maternal resveratrol supplementation ameliorated these effects in an F1 sex dependent manner.

Identification of determining factors size and shape Simbal cattle and Brahman Cross cattle in Pamenang Barat Merangin district

This study aims to determine the determinants of size and shape of Simbal and Brahman Cross cattle in the West Pamenang sub-district, Merangin district. The method of this study was a survey with a purposive sampling technique. Sampling criteria included: Sample of each breeds consisted of 60 Simbal and 60 Brahman Cross cattle aged 1-2 years. Data observed included: body length, shoulder height, chest circumference, inside chest, chest width, canon circumference, hip height, body weight, and body weight gain. The differences in body measurements between Simbal and Brahman Cross cattle analyzed by T-test. Identification of the determinants of size and shape of Simbal and Brahman Cross cattle were analyzed using Main Component Analysis method. The results of this study were body weight, body weight gain, and body measurements of Simbal cattle were significantly different (P &lt;0.05) higher than Brahman Cross cattle, both male and female. Simbal cattle and male Brahman Cross cattle were significantly different (P &lt;0.05) higher than females. The conclusion shows that body weight, body weight gain, and body measurements of Simbal cattle are higher than Brahman Cross cattle. Male body weight gain was higher than females in both Simbal and Brahman Cross cattle. The determining factor for the body size of Simbal and Brahman Cross cattle is chest circumference, while the determining factor for body shape of Simbal cattle is the height of shoulders, while Brahman Cross is body length. The highest correlation between body measurements and body weight in Simbal and Brahman Cross cattle, both male and femal is chest circumference (LD).

Haematological parameters in relation to age, sex and body weight of free-range village chickens (Gallus gallus domesticus) in Maiduguri, Nigeria

The haematology of animals including chickens is an important indicator of their health status. It is therefore considered a useful tool in clinical diagnosis. The aim of this study was to investigate the haematological parameters of apparently healthy free range domesticated chickens as influenced by their sex, age and body weights. Blood samples taken from the jugular veins were analysed using standard haematological protocols. PCV, haemoglobin concentration, TRBC, TWBC, differential leukocyte counts, erythrocytes indices (MCV, MCHC and MCH) were measured. The study showed that there were no significant (P&gt;0.05) age-dependent variation in all the measured haematological parameters. However, sex had effect on PCV, TRBC and heterophil count. Male chickens had higher PCV value of (34.4%) and TRBC value of (2.4 × 1012/L) as compared to female chickens with PCV value of (30.5%) and TRBC value of (2.1 × 1012/L). On the other hand, female chickens had higher mean heterophil value of (22.0 × 109/L) and MCHC value of (29.5g/dl) as compared to male chickens with mean heterophil and MCHC values of (16.8 × 109/L) and (25.7g/dl) respectively. There was significant (P&lt;0.05) increase in PCV with increasing body weights in males but not in females. The MCHC decreased significantly (P&lt;0.05) as the body weight increased also in males but not in females. The results of the study indicate that sex and body weights influenced normal haematological parameters of apparently healthy free range domestic chickens in the study environment. Keywords: Age, body weight, haematology, sex, village chicken.

Genetic parameter estimation for growth traits in males of Karan fries cattle

The significance of sire selection in the herd is reflected by the total genetic gain through sire-to-sire and sireto- dam pathways contributing greater than 50% contribution. The present investigation was carried out to estimate genetic parameters of males of Tharparkar (TP) and Karan-Fries (KF) cattle. Data on body weights of Tharparkar and Karan Fries males born during the period 199-2012 at ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, India were utilized in the present study. The genetic and phenotypic correlations among different body weights were estimated from the analysis of variance and covariance among sire groups. Paternal half-sib correlation method was used to estimate heritability of growth traits. Heritability estimates in KF were found to be of moderate to high magnitude, as 0.23±0.06, 0.43±0.10, 0.28±0.10, 0.36±0.11, 0.39±0.12, 0.38±0.14, 0.27±0.19 for weight at birth and weight at monthly interval up to six months, respectively. Genetic and phenotypic correlations were found to vary from moderate to high among the traits. Our study revealed that the magnitude of heritability indicates a high scope of further improvement in Karan Fries. Selective breeding and positive genetic correlations should be considered while designing selection programs.

Growth curves of campolina horses using nonlinear models

The Campolina horse breed stands out for its gait, its tall size, harmonious and curvaceous features, it is used in sports, leisure and service. The study of animal growth curves based on nonlinear models has often been used as a method for analyzing the development of several species, however, there are no reports of a study on modeling the growth curve in this breed. This present study aimed to evaluate the fit of the Logistical, Gompertz, von Bertalanffy and Brody models for the withers height (WH) and body weight (BW) in males and females, in addition to study the first to fourth order derivatives of the growth model aiming to identify relevant points of the growth curve. The experimental data were obtained from an agricultural show in Belo Horizonte, State of Minas Gerais, in 2012. A total of 356 Campolina horses were measured, 114 males and 242 non-pregnant females, which were divided into 16 age classes (7 to 203 months) and sex. Each animal was measured only once, characterizing a cross-sectional data collection study. The models were compared according to the following criteria for quality of fit: R2, AICc and MAPE. All adjustments and graphs constructed were analyzed by the statistical software R. The estimated asymptotic parameter for females in body weight were higher than those for males, indicating that they reach a higher adult weight, which may be due to different dietary practices, physiological conditions and physical conditioning between sexes. The maturity index of males was higher than for females, suggesting that the growth of males is more accelerated for withers height. The Gompertz model was chosen as the most suitable for the description of the growth curve in body weight and withers height of the Campolina breed horses because it presents best evaluators of quality and captures more influential points from its 1st to 4th order derivatives. Withers height develops faster than body weight. Some of the relevant points of equine growth still occur in the prenatal and lactation stages of foals.

Precocious puberty in male wild boars: a possible explanation for the dramatic population increase in Germany and Europe.

BackgroundThe wild boar population in Europe is steadily growing, one of the reasons for this increase probably being the high reproductive potential of this large mammal. Population management is important to stabilise wild boar numbers and a great deal of attention is focusing on the reasons, which might contribute to the high reproductive rates. Understanding the timing of puberty attainment provides information required for proper management practices. Knowledge of the earliest expected time of sexual maturation in male wild boars is limited, research being mostly focused on females. Previous hunting references indicate that sexual maturity in males occurs in the second year after birth. In contrast, male domestic pigs become sexually mature from about seven months of age. Thus, aims of this study were to investigate (1) whether there is a physiological ability for reproduction also in male wild boars of a younger age and (2) whether the body weight of wild boar males has a more important role than age in driving the maturation of the testis.MethodsMale wild boar individuals were sampled during hunting drives in the eastern part of Lower Saxony in Germany. Testes with epididymides from 74 males were collected and prepared for histological examination and immunohistochemistry. The reproductive status could be ascertained based on development/occurrence of different germ cell populations using histology and based on the immunohistochemical detection of the anti-Müllerian hormone and androgen receptor.ResultsIn this study, male wild boars aged nine to ten months already passed puberty and were able to reproduce if they had reached the appropriate body condition of about 29 kg dressed weight. Immunopositivity to the anti-Müllerian hormone in Sertoli cells was evident only in prepubertal animals and decreased with the onset of puberty. No immunoreaction was evident at postpuberty. The androgen receptor was detected in Sertoli cells, peritubular cells and Leydig cells, surprisingly already in Sertoli cells of prepubertal wild boars as well depending on body weight. Moreover, two-thirds of young males aged about ten months were precociously reproductively mature, showing histologically the presence of spermatozoa in testes and epididymides.ConclusionsAs piglets are mostly born in spring, also these young male individuals could target the heat of female wild boars in the winter months, resulting in the observed population increase. Therefore, a reduction in wild boar numbers should also focus on piglets of both sexes.

The Bidirectional Relationship between Body Weight and Depression across Gender: A Simultaneous Equation Approach

Purpose: This study investigates the bidirectional relationship between body weight and depression for both males and females in the U.S. Methods: Data are drawn from the 2019 Behavioral Risk Factor Surveillance System (BRFSS), and a simultaneous ordered probability system is estimated with maximum likelihood estimation (MLE) to accommodate the two-way causality between depression and body weight categories. The variable of depression is measured by individuals’ past depressive records and current mental health status. Results: Depression and body weight are found to affect each other positively for both males and females on average. In a randomized population, the results of average treatment effects suggest significant body weight differences between depressed and non-depressed individuals. Age and other sociodemographic factors affect body weight differently between genders and between the people with depression and those without. Conclusion: The positive bidirectional relationship between body weight and depression is found. The effect of depression on body weight is significant among both males and females in a randomized population, and females who experience depression are most likely to be obese and less likely to have normal weight compared to females without depression. The risks of overweight and obesity are high among people who are less educated or unable, who have poor health statuses, and who had high blood pressure.

Cardiometabolic consequences of maternal hyperandrogenemia in male offspring.

Polycystic ovary syndrome (PCOS) in women is characterized by hyperandrogenemia, obesity, and oligo‐ or anovulation. In addition, women with PCOS are often obese, with insulin resistance, hyperlipidemia, and elevated blood pressure. The cardiometabolic consequences for the male offspring of maternal hyperandrogenemia are unclear. The present studies tested the hypothesis that male offspring of a rat model of PCOS would develop cardiometabolic disease as adults. Female Sprague–Dawley rats (hyperandrogenemic females (HAF)) were implanted with dihydrotestosterone or placebo pellets (controls) at 4 weeks of age, and were mated at 10–12 weeks and allowed to lactate their offspring after birth. Body weights in male HAF offspring were lower at birth than in controls until postnatal day 4, but body weights remained similar between male control and HAF offspring from 2 to 8 weeks of age. However, at 16 weeks of age, body weight was lower in HAF male offspring, but there were no differences in fat mass or lean mass factored for body weight in HAF males, compared to controls. Plasma total cholesterol and HDL and proteinuria were higher and nitrate/nitrite excretion was lower in male HAF offspring than in controls. Baseline blood pressure was similar between HAF male offspring and controls, but HAF offspring had an exaggerated pressor response to angiotensin II infusion. These data suggest that adult sons of PCOS mothers may be at increased risk of cardiometabolic disease.

Effects of two water-based exercise programs on body weight and blood lipid parameters in elderly obese males with a sedentary lifestyle

Background and aimsThe consequences of exercise on atherogenic features are inconsistent. This study was aimed to examine the impacts of two water-based exercise programs (interval vs. continuous) without dietary intervention on body weight and blood lipid parameters, and its potential interactive effects and the activity of cholesteryl ester transfer protein (CETP) in obese older men with a sedentary lifestyle. MethodsSixty-two sedentary older men, who came to consult on weight loss to the Helal Ferdows Sports Complex, Iran, in 2018, volunteered for participation in this quasi-experimental study. Participants derived from a convenience sample were assigned at random into two aquatic exercise programs: Continuous training (CT) with moderate to vigorous-intensity and interval training (IT) with high-intensity. Both programs continued three months, three times a week, for 60-min each time. Comparing groups and times (pre- and post-intervention) was carried out using the Wilcoxon Nonparametric Test. The following assessments were completed pre- and post-intervention: body weight, body mass index (BMI), CETP activity, and blood lipid parameters. ResultsNo significant differences were observed when comparing the variables investigated within and between the groups’ pre- and post-intervention (P ˃ 0.05), except for body weight and BMI in the CT after the intervention (respectively, 0.6 kg, P = 0.04; 0.4 kg m−2, P = 0.03). ConclusionsBoth water-based exercise programs, without interference in nutrition, do not reveal a significant improvement in atherogenic features. The CT program detected modest but significant influences on body weight and BMI of obese older men with a sedentary lifestyle.

Effects of different selection strategies on subsequent breeder performance, slaughter, and carcass traits of Japanese quail

The present study evaluated the effect of different selection strategies in four closebred flocks (CBF) at three ages on subsequent breeder performance and carcass traits. A total of 540 Japanese quails already selected for higher body weight from four CBF (Major, Kaleem, Saadat, and Zahid) at three ages (10, 12, and 14 weeks) were subjected to 3 selection strategies (i.e. pedigree, mass selection, and randombred control). In pedigree selection, 108 birds with higher body weight were selected with full pedigree record whereas, in the mass selection, 324 birds with higher body weight were selected to be the parents of the next generation. However, in randombred control groups, 108 birds were selected without following any selection. The effect of selection strategies in parents of Japanese quails at 3 ages from 4 CBF was measured on subsequent breeder performance and carcass traits. Regarding productive performance, pedigreebased selected birds had the highest value for female body weight, average daily and fortnightly feed intake and average egg weight, whereas the mass selected birds revealed significantly higher production % and improved FCR (per dozen eggs and kg egg mass). Among different age groups, 14-week-old parents had the highest male and female body weight. However, no significant effect of different closebred flocks on production performance was observed in the present experiment. In conclusion, the pedigree-based and mass selection had a pronounced effect on productive performance and carcass traits, hence, can be an effective tool in the breeding of Japanese quails.

PROTECTIVE EFFECTS OF FLAX SEED OIL ON BODY WEIGHT CHANGES CAUSED BY CAFFEINATED ENERGY DRINK IN ADULT MALE ALBINO RATS

Flax seed oil has proven dynamic multi systemic effects since ancient times. Consumption of caffeinated energy drinks has also been increased among youth in order to increase mental and physical performance. Due to their widespread usage, hazardous effects on various systems of human body have been reported. &#x0D; Aims &amp; Objectives: To evaluate the protective effects of flax seed oil on caffeinated energy drink induced changes in adult male albino rat body weight.&#x0D; Place &amp; Duration of Study: This study was conducted in FPGMI, Shaikh Zayed Hospital Lahore for 8 weeks.&#x0D; Material &amp; methods: 32 adult male albino rats average weight (250-300g) were randomly divided into four groups of 8 animals each. Group A (Control) received corn oil 5ml/kg body weight by gavage in addition to basal diet daily for 8 weeks. Group B (Experimental) were fed on caffeinated energy drink (15ml/kg body weight) and corn oil (5ml/kg body weight). Group C (Experimental) received caffeinated energy drink (15ml/kg body weight) and 40% of flax seed oil (5ml/kg body weight), while group D (Experimental) received caffeinated energy drink(15ml/kg body weight) and 60% flax seed oil (5ml/kg body weight) daily for 8 weeks respectively. The animals were weighed before and after experiment.&#x0D; Results: The mean body weight of rats before experiment was insignificant (p = 0.945). After experiment the mean body weight of experimental group B, C and D was increased as compared to control group A, but statistically it was insignificant ( p = 0.319) however, percentage body weight gain was significant( p = 0.003).&#x0D; Conclusion: Flax seed oil alleviated altered body weight caused by caffeinated energy drink in adult male albino rats

218-LB: Age, Sex, and Fat Mass Affects Erythropoietin Metabolic Response in Mice

Erythropoietin (EPO) is required for erythrocyte production. However, EPO receptor (EpoR) extends beyond erythroid tissue and ΔEpoR mice with EpoR restricted to hematopoietic tissue show an increase in fat mass. At 3 months of age, ΔEpoR mice show no difference in body weight compared with wild type (WT, C57BL/6) mice. By 6 months of age, male body weights of ΔEpoR mice are greater than WT mice, and at 8+ months body weights of male and female ΔEpoR mice are greater than their WT counterparts. To assess EPO activity on glucose homeostasis and fat mass, mice were fed a high fat diet (HFD, 60% kCal fat) and treated with EPO (3000U/kg 3X per week) or saline for 3 weeks at age 3, 6, and 8+ months. EPO increased hematocrit in all mice. EPO treatment in male WT mice reduces weight and fat mass at 6 and 8+ months of age, when their fat mass is 35% or greater with saline treatment. Female WT mice treated with EPO do not show significantly less weight or fat mass at any age. EPO treatment does not affect weight or fat mass in ΔEpoR mice. With HFD feeding and EPO treatment in 3 month old mice, body weights are not different between WT and ΔEpoR mice although fat mass is significantly higher in ΔEpoR mice. At 6 months, body weight and fat mass in males are greater in ΔEpoR than WT, while ΔEpoR females show no difference in body weight but significantly more fat mass compared with WT females. In the older mice (8+ months old), both sexes of ΔEpoR mice are significantly heavier and fatter than WT mice. EPO treatment improves glucose tolerance in female ΔEpoR mice at 3 and 6 months but not at 8+months, whereas male ΔEpoR mice only showed improvement at 3 months, not at 6 and 8+months. In male and female WT mice, EPO improves glucose metabolism at 3 and 6 months, and to a lesser extent at 8+ months with greater improvement in mice with &amp;lt;35% fat mass. These data show that EPO regulates metabolism in a sex and age dependent manner mediated via EpoR expression in non-hematopoietic tissue and is influenced by level of fat mass. Disclosure H. Rogers: None. C. T. Noguchi: None.

Effect of testic brahman (Bos indicus) mixed with commercial feed and different time for genital of male and female in Tilapia (Oreochromis niloticus)

The Tilapia is hermaphrodite fish, and from larva they can changing of genital for examples male or female. Male tilapia has faster growth rate than female tilapia. Many technologies were produces genital of fish male dominant population. But to produces that genital were need to prepare for the diet, ones of that were give of the feeding. These results were used of the brahman testicles and mixed with commercial feed, the data were using by immersion method. The results were found value of male seen the larvae compared for the fry tilapia. To give that feed were used at the different time of immersion treatments. The time of immersion was separated to P1 (16 hours), P2 (32 hours), P3 (48 hours) and P0 as (control). The results were observed of male tilapia, body weight, length and water quality parameters. The results of immersion time shown of tilapia larvae very significant effect compared the female. The results were found from the age of 60 days, immersion time of P0 was observed with value of 52.83%, P1 o f85.71%, P2 of 74.49% and P3 was observed and value of 62.41%.