The term "pyogenic infection" describes an infectious agent-induced localized inflammation and pus production caused by a buildup of dead leukocytes. 120 patients' specimens were gathered and grown on blood agar and MacCokey agar. Biochemical tests were used to identify colonies grown in these conditions, and subsequently their antibiotic susceptibility was examined. When combined with the patient's medical history, these data showed how common pyogenic infections were in BIHS General hospital, Dhaka. Within 86.67% of the Positive pyogenic infection were due to bacterial agents, with Staphylococcus aureus (23.07%), Escherichia coli (15.39%), Klebsiella (15.39%), Proteus (15.39%), Pseudomonas (7.69%), Enterococci (7.69%), Citrobacter (7.69%), Enterobacter (7.69%) being the major pathogens. Female was predominant than male which was 72 (60%) cases and 48 (40%) cases respectively. The most common age group for both the male and female groups was 40–60 years old, with 16 (13.33%) and 64 (53.33%) instances, respectively. In conclusion, Staphylococcus aureus is the most often isolated bacterium following aerobic culture of pus. Nonetheless, there are fewer gram-positive cocci than gram-negative bacilli.