Major Components Of Oil Research Articles

Chemical Composition, Antibacterial Activity using Micro-broth Dilution Method and Antioxidant Activity of Essential Oil and Water Extract from Aerial Part of Tunisian Thymus algeriensis Boiss. & Reut.

In this study, we investigated the chemical composition, antioxidant and antibacterial activities of essential oil and aqueous extract from Thymus algeriensis Boiss. & Reut growing in Tunisia. GC/MS analysis of essential oil from the aerial part of the plant led to identifying 54 constituents representing 96.87 % of the total oil composition. Monoterpenes represented the major components of the essential oil. On the other hand, the aqueous extract and the essential oil were screened for their antioxidant and antibacterial activities. The latter was assessed against Gram-negative and Gram-positive bacterial strains using the broth dilution micro method for the determination of antibacterial activity. The antioxidant activity was evaluated using the DPPH scavenging activity and Ferric Reducing/Antioxidant Power (FRAP) assays. The essential oil was found to be more active in antibacterial screening (MIC = 0.54 μg/mL). Whereas the aqueous extract exhibited the most potent antioxidant activity with (IC50 = 0.04 μg/mL). UHPLC ESI(-)-HRMS/MS analysis of the aqueous extract allowed tentative identification of its constituents and showed that it is rich in phenolic compounds. Luteolin-glucuronide was found to be the most abundant compound followed by Vicenin-2 and Apigenin-diglucuronide.

Biological activity of essential oil from Foeniculum vulgare

Abstract Foeniculum vulgare Mill. is a medicinal plant, used as a flavouring agent. The essential oil from F. vulgare has potential antimicrobial and insecticidal effects, and can be used in food industry in order to protect the food resources and food products against microbial and pest’s contamination. The aim of the research was to characterize the volatile components of F. vulgare essential oil by Gas Chromatography/Mass Spectrometry (GC/MS) and Gas Chromatography (GC-FID) and to observe the antimicrobial activity by disk diffusion method and in vapour phase. Also, insecticidal activity of the vapour phase of the essential oil of F. vulgare was detected. We found that major components of the essential oil from F. vulgare were trans-anethole (73.6%), fenchone (6.0%), and limonene (5.7%). Antimicrobial activity on gram-positive, gram-negative, and yeasts was weak in liquid phase, but vapour phase showed stronger activity against B. subtilis at the concentration 250 μL.L−1 (98.65% of bacterial growth inhibition). Vapour phase of essential oil was effective against insects, where 25% concentration had 80% lethality.

Anti-Inflammatory and Antimicrobial Volatile Oils: Fennel and Cumin Inhibit Neutrophilic Inflammation via Regulating Calcium and MAPKs.

Neutrophilic inflammatory diseases, such as chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), or psoriasis, exert a huge burden on the global health system due to the lack of safe and effective treatments. Volatile oils from terrestrial plants showed impressive therapeutic effects against disorders of the skin, digestive system, lungs, liver, metabolism, and nervous system. However, their effect on the immune system and neutrophil function is still elusive. Fennel, cumin, marjoram, lavender, caraway, and anise are the common nutraceuticals that are widely used in the Mediterranean diet. The volatile oils of these herbs were screened for various biological activities, including anti-inflammatory, anti-allergic, antimicrobial, and antiviral effects. Several oils showed anti-inflammatory and antimicrobial potential. Fennel (Foeniculum vulgare) and cumin (Cuminum cyminum) fruits' volatile oils significantly suppressed the activation of human neutrophils, including respiratory burst and the degranulation induced by formyl peptide receptor agonists fMLF/CB and MMK1 in the human neutrophils (IC50, 3.8–17.2 µg/ml). The cytotoxic effect and free-radical scavenging effects (ABTS, DPPH) of these oils did not account for the observed effects. Both fennel and cumin volatile oils significantly shortened calcium influx recovery time and inhibited phosphorylation of mitogen-activated protein kinases (p38, JNK, and ERK) expression. The gas chromatography–mass spectrometry analysis of these oils revealed the presence of estragole and cuminaldehyde as the major components of fennel and cumin volatile oils, respectively. Our findings suggested that cumin and fennel, common in the Mediterranean diet, hold the potential to be applied for the treatment of neutrophilic inflammatory diseases.

Commercial Energy Demand Forecasting in Bangladesh

Although both aggregate and per capita energy consumption in Bangladesh is increasing rapidly, its per capita consumption is still one of the lowest in the world. Bangladesh gradually shifted from petroleum-based energy to domestically sourced natural-gas-based energy sources, which are predicted to run out within next two decades. The present study first identified the determinants of aggregate commercial energy and its three major components of oil, natural gas, and coal demand for Bangladesh using a simultaneous equations framework on an annual database covering a period of 47 years (1972–2018). Next, the study forecast future demand for aggregate commercial energy and its three major components for the period of 2019–2038 under the business-as-usual and ongoing COVID-19 pandemic scenarios with some assumptions. As part of a sensitivity analysis, based on past trends, we also hypothesized four alternative GDP and population growth scenarios and forecast corresponding changes in total energy demand forecast. The results revealed that while GDP and lagged energy demand are the major drivers of energy demand in the country, we did not see strong effects of own- and cross-price elasticities of energy sources, which we attributed to three reasons: subsidized low energy prices, time and cost required to switch between different energy-mix technologies, and suppressed energy demand. The aggregate energy demand is expected to increase by 400% by the end of the forecasting period in 2038 from its existing level in 2018 under the business-as-usual scenario, whereas the effect of COVID-19 could suppress it down to 300%. Under the business-as-usual scenario, the highest increase will occur for coal (3.94-fold), followed by gas (2.64-fold) and oil (2.37-fold). The COVID-19 pandemic will suppress the future demand of all energy sources at variable rates. The ex ante forecasting errors were small, varying within the range of 3.6–3.7% of forecast values. Sensitivity analysis of changes in GDP and population growth rates showed that forecast total energy demand will increase gradually from 3.58% in 2019 to 8.79% by 2038 from original forecast values. Policy recommendations include capacity building of commercial energy sources while ensuring the safety and sustainability of newly proposed coal and nuclear power installations, removing inefficiency of production and distribution of energy and its services, shifting towards renewable and green energy sources (e.g., solar power), and redesigning subsidy policies with market-based approaches.

1,8-Cineole Affects Agonists-Induced Platelet Activation, Thrombus Formation and Haemostasis.

1,8-cineole, a monoterpenoid is a major component of eucalyptus oil and has been proven to possess numerous beneficial effects in humans. Notably, 1,8-cineole is the primary active ingredient of a clinically approved drug, Soledum® which is being mainly used for the maintenance of sinus and respiratory health. Due to its clinically valuable properties, 1,8-cineole has gained significant scientific interest over the recent years specifically to investigate its anti-inflammatory and antioxidant effects. However, the impact of 1,8-cineole on the modulation of platelet activation, thrombosis and haemostasis was not fully established. Therefore, in this study, we demonstrate the effects of 1,8-cineole on agonists-induced platelet activation, thrombus formation under arterial flow conditions and haemostasis in mice. 1,8-cineole largely inhibits platelet activation stimulated by glycoprotein VI (GPVI) agonists such as collagen and cross-linked collagen-related peptide (CRP-XL), while it displays minimal inhibitory effects on thrombin or ADP-induced platelet aggregation. It inhibited inside-out signalling to integrin αIIbβ3 and outside-in signalling triggered by the same integrin as well as granule secretion and intracellular calcium mobilisation in platelets. 1,8-cineole affected thrombus formation on collagen-coated surface under arterial flow conditions and displayed a minimal effect on haemostasis of mice at a lower concentration of 6.25 µM. Notably, 1,8-cineole was found to be non-toxic to platelets up to 50 µM concentration. The investigation on the molecular mechanisms through which 1,8-cineole inhibits platelet function suggests that this compound affects signalling mediated by various molecules such as AKT, Syk, LAT, and cAMP in platelets. Based on these results, we conclude that 1,8-cineole may act as a potential therapeutic agent to control unwarranted platelet reactivity under various pathophysiological settings.

Antioxidant activities and the chemical composition of the essential oil from Eucalyptus pulverulenta (Baby Blue Eucalyptus) grown in The Nilgiris

Eucalyptus pulverulenta (Baby Blue Eucalyptus) originated from Australia is one of the rare species of Eucalyptus family (Myrtaceae) and well adapted to the hilly regions of India. Apart from the cut foliage utility of the plant, its leaves have a pleasant aroma like most of the members of Myrtaceae. This study was designed to extract essential oil using hydro distillation method. Four different types of samples were used (viz. tender leaves, mature sticks, mature leaves along with sticks and mature leaves alone) among which matured leaves were found to have maximum amount of essential oil. GC-MS analysis of the essential oil was carried out to analyse the chemical composition. The major component of the essential oil was found to be 1, 8 cineole. The antioxidant ability of the essential oil to scavenge the free radicals was assessed using three antioxidant assays: DPPH assay, ABTS assay and FRAP assay.

Chemical Composition, Antibacterial and Antifungal Activity of the Essential Oil from Cistus ladanifer L.

Cistus ladanifer L. is a plant widely used in folk medicine to treat various illnesses. This study aims to evaluate the effect of the plant flourishing time harvest on the chemical composition and the antimicrobial effect of its essential oil. Chemical analysis of the essential oil was carried out using gas chromatography-mass spectrometry (GC-MS). The antibacterial and antifungal proprieties were tested against four selected bacteria (Staphylococcus aureus, Salmonella Typhi, Escherichia coli, and Acinetobacter baumannii) and nine fungi (Yeasts (Candida tropicalis, Candida glabrata, Candida dubliniensis, Candida sp., Rhodotorula rubra, Cryptococcus neoformans) and molds (Penicillium sp. (P), Fusarium sp. (F), Aspergillus niger (A. niger)), respectively. The essential oil of C. ladanifer demonstrated a powerful antibacterial activity with an inhibition zone of 55 ± 0.22 mm for Staphylococcus aureus, 42 ± 0.11 mm for Escherichia coli, 35 ± 0.27 mm for Acinetobacter baumannii (Full resistant to antibiotics) and 30 ± 0.25 mm for Salmonella Typhi. It also inhibited all tested bacteria at 10 µL/mL. For the antifungal activity test, C. tropicalis and C. neoformans appeared to be the most sensitive strains to the essential oil with an inhibition zone of 13 mm, followed by R. rubra and Penicillium sp. (12 mm), then C. dubliniensis and C. glabrata (11 mm). The chemical analysis of the essential oil by GC-MS revealed that the major components of the essential oil were viridiflorol (17.64%), pinocarveol (11.02%), bornylacetate (9.38%), and ledol (8.85%). C. ladanifer exhibited a remarkable antimicrobial activity that could be more exploited to develop targeted natural remedies against specific diseases.

Composition characterization and biological activity study of Thymbra spicata l. var. spicata essential oil

The current research aimed to determine and report in vitro antioxidant, antimicrobial, antibiofilm, cytotoxic, anti-cholinesterase, and anti-diabetic properties and the stability of the major component of basic oil of Thymbra spicata var. spicata through different phases as theoretically. Essential oil exhibits potential biological activities because of the multiple components it contains.In the current research, the evaluation of Thymbra spicata essential oil antioxidant properties was conducted utilizing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) radical scavenging activity.Antimicrobial activity was assessed from minimum inhibition concentration (MIC) using the technique of microdilution and cytotoxicity activity was evaluated by MTT assay through MCF-7 and PC3 human cancer cell lines.Consequently, Cytotoxic activity was evaluated by means of MTT assay utilized. The essential oil was detected to have 340 µg/mL inhibiting influence on the growth of PC3 prostate cancer cells with IC50 value. Also, the T. spicata plant was observed to significantly repress the enzymes, namely acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase. IC50 values of enzymes were obtained 0.23 µg/mL for AChE, 1.64 µg/mL for BChE, 7.78 µg/mL for α-glycosidase. It was concluded that this plant may be used for Alzheimer's and diabetes disease.

Antifungal activity of wild bergamot (Monarda fistulosa) essential oil against postharvest fungal pathogens of banana fruits

This study aimed to examine the antifungal activity of wild bergamot (Monarda fistulosa) essential oil on Colletotrichum musae and Lasiodiplodia theobromae, the causative organisms of anthracnose and crown-rot diseases of banana fruits, respectively. Chitin synthase is a promising target for antifungal compounds, since it is involved in synthesizing chitin, which forms a major proportion of the fungal cell wall. Disc volatilisation method was employed to assess the in vitro antifungal activity of the oil. The vapours of this essential oil at 4 μL per Petriplate exhibited 100% growth inhibition of both the fungal pathogens, and at 66.66 μLL−1 , it significantly (P <0.05) reduced the incidence and severity of anthracnose and crown-rot diseases in artificially wounded and infected fruits. The chemical composition of wild bergamot essential oil was determined by GC-MS technique and the components of the essential oil were docked against chitin synthase to determine the components responsible for antifungal activity. Chitin synthase was modelled by de novo approach due to non-availability of the 3D structure. The in silico techniques such as molecular docking and conceptual DFT revealed that the major components of the essential oil namely thymol, carvacrol and cinnamyl carbanilate manifested the best antifungal activity. This illustrates effective inhibition of the major postharvest diseases of banana by wild bergamot essential oil.

Not Only a Weed Plant-Biological Activities of Essential Oil and Hydrosol of Dittrichia viscosa (L.) Greuter.

With the increasing interest in obtaining biologically active compounds from natural sources, Dittrichia viscosa (L.) Greuter (Asteraceae) came into our focus as a readily available and aromatic wild shrub widely distributed in the Mediterranean region. This work provides a phytochemical profile of D. viscosa in terms of parallel chemical composition in the lipophilic fraction (essential oil) and the water fraction (hydrosol). GC-MS analysis identified 1,8-cineole, caryophyllene oxide, α-terpenyl acetate, and α-muurolol as the major components of the essential oil, while in the hydrosol p-menth-1-en-9-ol, 1,8-cineole, linalool, cis-sabinene hydrate, and α-muurolol were the major volatile components. 3,4-Dihydroxybenzoic acid was found to be the predominant compound in the hydrosol composition by HPLC analysis. The antimicrobial potential of both extracts was evaluated against thirteen opportunistic pathogens associated with common skin and wound infections and emerging food spoilage microorganisms. The antimicrobial activity of the essential oil suggests that the volatiles of D. viscosa could be used as novel antimicrobial agents. The antiproliferative results of D. viscosa volatiles are also new findings, which showed promising activity against three cancer cell lines: HeLa (cervical cancer cell line), HCT116 (human colon cancer cell line), and U2OS (human osteosarcoma cell line). The decrease in GSH level observed in hydrosol-treated HeLa cells suggests oxidative stress as a possible mechanism of the antiproliferative effect of hydrosol on tumor cells. The presented results are also the first report of significant antiphytoviral activity of hydrosol against tobacco mosaic virus (TMV) infection. Based on the results, D. viscosa might have the potential to be used in crop protection, as a natural disinfectant and natural anticancer agent.

Chemical Components and Antimicrobial Activities of Tea Tree Hydrosol and Their Correlation With Tea Tree Oil

Tea tree hydrosol (TTH) is the aqueous phase of the distillate from the steam distillation process of the twigs and leaves of Melaleuca alternifolia. In this study, the chemical components of TTH were analyzed by gas chromatography–mass spectrometry (GC–MS) and gas chromatography–flame ionization detector (GC-FID). The major components were terpenoids (1.53 mg/mL), including monoterpenoids and sesquiterpenoids. The antibacterial activity of TTH was assessed by paper disc agar diffusion and broth dilution methods. Terpinen-4-ol (MIC: 0.31-0.62 mg/mL), 2- endo-hydroxy-1,8-cineole (MIC: 1.25-2.50 mg/mL) and p-menth-3-ene-1,2-diol (MIC: 0.62-2.50 mg/mL) were the main antibacterial components of TTH. The results showed that the major components of TTH and tea tree oil (TTO) had the same parent structures, but different degrees of oxidation. Terpinen-4-ol was the most abundant component in both TTH (624.2 μg/mL) and TTO (35-48%), and played a major role in antibacterial activities. This is the first report on the chemical components and antibacterial activities of TTH and their correlation with TTO, which demonstrates the potential application of TTH as a natural antimicrobial agent in health care products.

Extraction of Oleic Acid from Animals Oil and Its Anti-inflammatory Effect on Network Pharmacology

The purpose of this paper is to explore the possible mechanisms of anti-inflammatory and scar repair by Mongolian horse oil. We used TCM database and literature mining to collect active compounds of horse oil and used Swiss TargetPrediction and SuperPred server to find targets of compounds. Anti-inflammatory drug targets were collected through the CTD database. Go annotation of targets and KEGG pathway were enriched and analyzed through Metascape database platform. Molecular docking between active ingredients and targets was verified by AutoDock software. Metascape analysis revealed that the key candidate targets were significantly enriched in a number of pathways associated with inflammatory pathology. The results of molecular docking showed that oleic acid, a major component of animals oil, could influence the regulatory functions of TNF, NGF, IL6, IL1B, Jun, and CDK1. This suggests that animals oil can regulate the development of inflammation through its active ingredient, oleic acid, and can influence the expression of multiple signaling pathways, with theoretical endogenous interactions with TNF, NGF, IL6, IL1B, JUN, and CDK1 proteins.

Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene

BackgroundAloysia gratissima (Verbenaceae), popularly known as bee-brush or whitebrush, has been widely used in Brazilian folk medicine as analgesic, expectorant and antimicrobial. Phytochemical studies have identified β-caryophyllene as one of the major components of the essential oil of this plant. This bioactive sesquiterpene has been shown to have anti-inflammatory and antibacterial activities. PurposeThe present study aimed to characterize the chemical profile and evaluate the antibacterial and antibiotic-enhancing activities of the essential oil obtained from Aloysia gratíssima (EOAG) and β-caryophyllene. MethodsThe phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial activity against Pseudomonas aeruginosa 24, Staphylococcus aureus 10, and Escherichia coli 06 was assessed by determining the minimum inhibitory concentration (MIC) using the broth microdilution method. The potentiation of the antibiotic activity by the EOAG and the β-caryophyllene was performed using the MIC evaluation of the antibiotic alone or in association with the essential oil and its main compound. To evaluate the minimum reduction of the drug concentration necessary, the Dose Reduction Index (DRI) was calculated. ResultsA reduction in the MIC of the antibiotics against strains treated simultaneously with the essential oil or β-caryophyllene was observed. The GC-MS analysis of the EOAG identified 30 compounds, including β-caryophyllene as the major component. Both OEAG and β-caryophyllene presented antibacterial activity against S. aureus, in addition to potentiating the action of norfloxacin against S. aureus, P. aeruginosa, and E. coli. ConclusionsThese substances also reversed the antibiotic resistance to gentamicin and erythromycin against of S. aureus and P. aeruginosa, respectively.

Direct and simultaneous analysis of lipase-catalyzed hydrolysis of high-oleic oil model by chiral stationary phase HPLC-ELSD

A novel HPLC-based method for direct separation of trioleoylglycerol (TOG), a major component in high-oleic oils, and its seven hydrolysis products (i.e., oleic acid, monooleoylglycerol (MOG) and dioleoylglycerol (DOG) isomers) was established using a chiral stationary phase column, Chiralpak IA. Within 20 min, all species including enantiomeric MOG (1-sn-MOG and 3-sn-MOG) and DOG (1,2-sn-DOG and 2,3-sn-DOG) were baseline-resolved with resolution factors over 1.5 between adjacent peaks. The established method was used for investigating the integral stereoselectivity, which is the selectivity concerning all hydrolysis steps, of lipase from Pseudomonas fluorescens (PFL) with TOG as substrate. The time-course of DOGs and MOGs indicated that PFL had selectivity for TOG hydrolysis in the order of sn-1, sn-2, and sn-3 position, while it preferred to hydrolyze 2,3-sn-DOG over 1,2-sn-DOG. Being rapid and accurate to evaluate integral stereoselectivity, this method could promote the development and application of lipases with target stereochemistry in the food industry.

The differences between Froriepia subpinnata (Ledeb.) Baill. and Pimpinella anisum L. commonly named as anarijeh based on major components of the essential oil; a marker for resolve ambiguities

The differences between <i>Froriepia subpinnata</i> (Ledeb.) Baill. and <i>Pimpinella anisum</i> L. commonly named as anarijeh based on major components of the essential oil; a marker for resolve ambiguities

Planting-date and cutting-time affect the growth and essential oil composition of Mentha × piperita and Mentha arvensis

Peppermint (Mentha × piperita L.) and Japanese mint (Mentha arvensis L.), as the prominent menthol bearing species are widely cultivated and have a considerable share in the global essential oil trade. In this research, the effects of two different planting dates (spring and autumn), diverse cutting time, and alluvial soil conditions were investigated on the growth and essential oil compounds of M. × piperita L. var. Black Mitcham and M. arvensis var. piperascens Holmes in Çukurova Region/Turkey, during 2011−2014. According to the results, despite the relative superiority of M. arvensis; no significant difference was recorded in the growth potential of two species. However, the content and yield of M. arvensis essential oil (4.8 % and 94 L ha−1) were substantially higher than M. × piperita (2.8 % and 43 L ha−1). In terms of plant height, dry biomass, leaf dry weight, and essential oil yield, the spring planting date was significantly superior to autumn. Among the six cuttings, the data of the first cuttings were significantly higher than the second cuttings for each year. The major essential oil constituents of M. × piperita were menthol (22.5–44.6 %) and menthone (13.8–37.4 %). Overall, menthol was more abundant in the spring cultivation of M. × piperita. In contrast, menthone amounts were higher in autumn cultivation. Menthol (53.6–70.5 %) was the major compound of M. arvensis. Isomenthone (4.5–17.7 %), menthone (3.3–14.5 %), and α- pinene (3.6–11.1 %) were the other major oil components of M. arvensis. Menthol content significantly decreased from the fourth cuttings. Although a declining trend in the yield of species was observed over time, the results were acceptable considering the restrictive conditions of the alluvial soils. All in all, without imposing the limits on the cultivation areas of the strategic agricultural crops; the cultivation of medicinal plants would be advisable with the plenty of alluvial lands.

Phytotoxicity of essential oil from Piper nigrum L. on some selected food crops as a potential herbicide in Africa

Phytotoxicity of the essential oil (EO) of Piper nigrum L. against Solanum lycopersicum L., Zea mays L. and Vigna unguiculata L. was studied. The GC-MS analysis of the essential oil of P. nigrum shows that linalool (21.73 %) was the major component of the essential oil. The EO retarded the growth of the root and the shoot of S. lycopersicum, Z. mays and V. unguiculata. The inhibition of the root ranged from 64.8-82.8% after 120 hours with a concentration of 4 mL/L, while for the shoot, 100% inhibition was observed with 4 mL/L of the EO formulation after just 96 hours. The phytotoxic effect on the leaves was highest in V. unguiculata and least in S. lycopersicum. Phototoxicity was also observed when the EO formulations were applied to the root of the seedlings of Z. mays and V. unguiculata recorded 100% distress of the seedlings when 3 mL/L of the graded concentration was used after 24 hours. EO from P. nigrum is a potential bio-herbicide with a wide spectrum of use on plants.

Anti-inflammatory, antibacterial and antioxidant activities of Chenopodium ambrosioides L. (Chenopodiaceae) extracts

Objective: Chenopodium ambrosioides is an aromatic plant widely used in the Togolese traditional medicine. However, little is known about its pharmacological properties. The present study aimed to investigate the anti-inflammatory, antibacterial and antioxidant activities of its extracts. Methodology and results: Thus, an ethnopharmacological survey was undertaken in Kara town in the northern part of Togo to assess the main uses in the traditional medicine and Gas Chromatography coupled with Mass Spectral Analysis (GC-MS) was used to identify several compounds in the extracts. Antioxidant activity was evaluated by FRAP, ABTS, DPPH methods. Anti-inflammatory and antimicrobial activities were assessed by soybean lipoxygenase inhibition and in vitro broth micro dilution techniques, respectively. Propidium iodide cell death was evaluated by flow cytometry and in vivo toxicity was assessed using wistar rats. The ethnopharmacological survey revealed that traditional healers use C. ambrosioides to treat malaria, intestinal worms and inflammation in addition to healing wounds. The hydroethanolic extract had high content of total phenols (324.80±17.30 µgEAG/mg) and flavonoids (63.20±8.70 µgEQ/mg), however the highest antioxidant, antimicrobial and antiinflammatory activities were obtained with the essential oil. GC-MS analysis leads to identification of hydrocarbon monoterpenes such as 2carene, ortho-cymene and α-terpinene as the major components of the essential oil. All the tested extracts induced cell death (14.60 ± 9.23% at 2 µL/mL, 72.64 ± 15.92% at 200µL/mL and 89.50 ± 7.16 at 100 mg/mL for essential oil, hydrosol and hydroethanolic extract, respectively). Conclusion and application of results. The present study demonstrated various pharmacological activities of hydroethanolic extract, essential oil and hydrosol of leaves of Chenopodium ambrosioides. The essential oil had antioxidant, antimicrobial and antiinflammatory activities and could be the most active component of the leaves of Chenopodium ambrosioides. Our findings highlighted perspectives for the discovery of new medicinal molecules derived from plant extrats and confirmed certain practices of traditional healers. Keywords: Chenopodium ambrosioides, extract, pharmacological activities

Development and experimental validation of reactor kinetic model for catalytic cracking of eugenol, a potential bio additive fuel blend

Abstract An integrated kinetic model representing catalytic cracking of eugenol in a fixed bed reactor is developed. Eugenol, a major component of clove oil can act as the most potential bio additive fuel for improving the diesel quality thereby reducing the exhaust emission of the engine. The proposed integrated model includes four lump kinetic model which is plugged with catalyst deactivation model. The reactor design parameters are also included in the integrated model. The effectiveness of the proposed integrated model is compared with the conventional kinetic models and the results are presented. The proposed integrated model is validated against the real time data obtained by conducting an experiment in a real time setup with MoS2(Ni2P) Al-SBA-15(10) as the catalyst. The advantages of the proposed integrated model are highlighted.

Essential oil of Noni, Morinda citrifolia L., fruits controls the rice stem-rot disease without detrimentally affect beneficial fungi and ladybeetles

The use of botanical fungicides has been presented as a promising tool to replace the use of synthetic compounds, as the former seems to offer reduced risks to the environment and human health. Here, we characterized the major constituents and evaluated the potential of essential oils of Noni, Morinda citrifolia L. plants for controlling stem-rot disease caused by the fungus Sclerotium rolfsii Catt in Neotropical rice, Oryza sativa L., fields. Using in silico molecular predictions and toxicological approaches, we further assessed the essential oil selectivity against beneficial organisms such as the fungi Trichoderma asperellum Samuels, Lieckfeldt & Nirenbeand the ladybeetles Eriopis connexa Germar and Coleomegilla maculata DeGeer. Our results revealed that octanoic acid (64.0 %), butanoic acid (10.2 %), and hexanoic acid (8.6 %) were the major oil components. When applied at 30 mg/mL, the essential oil inhibited mycelial growth, whereas at 10 mg/mL, it inhibited the germination of all S. rolfsii sclerotia. The essential oil application at 25 mg/mL reduced disease severity in both curative (56.4 %) and preventive (71.2 %) methods. Essential oil applications (30 mg/mL) did not affect the beneficial fungus T. asperellum nor did it kill any ladybeetle. Our molecular docking analyses reinforced the essential oil selectivity actions predicting higher potential interactions between its major components and the tyrosine-tRNA ligase of S. rolfsii compared to those of T. asperellum. Collectively, our findings demonstrated that Noni essential oil could control S. rolfsii infestations without causing any detrimental effects on relevant beneficial organisms, highlighting its potential as an alternative fungicide.