Major Modifications Research Articles

Stability Analysis of a Weak-Grid-Connected Voltage-Sourced Rectifier Considering the Phase-Locked Loop Dynamics

The high bandwidth of a phase-locked loop (PLL) can deteriorate the small-signal stability of a voltage-sourced converter (VSC) under a weak grid condition. This instability can occur for a VSC operating as both a rectifier and an inverter, but the root causes can differ for the two. This study, through modal analysis, demonstrates that different approaches are required depending on the power flow direction to resolve the instability. Subsequently, an analysis of the rectifier dynamics is set as the primary target, and the instability characteristic is investigated via a new approach that utilizes the open-loop transfer function of the power control loop. Consequently, it is discovered that the high-bandwidth PLL reduces the reactive power compensation for the AC system and results in a severe voltage drop. A novel parallel voltage filter technique is proposed to overcome this instability. The performance of the proposed method is verified in the frequency- and time-domain analyses. The results indicate that the proposed method effectively improves stability without major modifications to the control system.

Difficulty, Discrimination and Pseudo - Guessing Indices of West African Examinations Council Core Mathematics Multiple Choice Items: Theoretical and Practical Implications of Using Item Response Theory

Viewing from the psychometric models of modern assessment, it is imperative to note that assessment has been undergoing major constructive modifications. One of the main modifications is the transition from the use of classical test theory (CTT) to modern test models (IRT) as well as methods used in test construction. This is to say that the pendulum swing in test construction techniques is from CTT to IRT. Clearly, the properties of IRT create several benefits in the area of test design and item banking over CTT. This paper estimated the IRT psychometric proprieties (difficulty-1PL, discrimination-2PL and guessing indices-3PL) of West African Examinations Council (WAEC) 2020 core mathematics objectives test examination in Ghana within the remit of IRT. The study revealed that averagely, the 2020 core mathematics objectives test examination were within the difficulty level (1PL) of the examines. In terms of discrimination level (2PL), the psychometric properties indicated that most of the items discriminated among the examinees. However, on the Pseudo-guessing parameter (3PL), it was found that most items were subjected to pseudo-guessing. Theoretically and practically, the way items are constructed could have influenced outcome. Therefore, we strongly advocate and recommend that item writers for examination body like West African Examinations Council (WAEC) should rigorously be trained to be more conscious of theoretical and practical implications of the guessing parameter. This will help in avoiding test items that are prone to pseudo-guessing.

Role of vinca alkaloids and their derivatives in cancer therapy

Vinca alkaloids and its derivatives like Vincristine, Vinblastine etc. isolated from Catharanthus roseus plants are widely used in the treatment of various types of cancers. Mode of action of Vinca alkaloids (vinblastine, vincristine, vinorelbine) is microtubule-stabilizing agents (MTAs) i.e., arrest the cell cycle via disrupting microtubule dynamics. Vincristine major side effect is neurotoxicity. Hoverer, Vincristine induce neuropathy in mice or rat, used as animal model to study effect of drugs or plants by various authors also reported in review literature. Vinca alkaloids and its derivatives were widely used drugs in combination regimens with cyclophosphamide, doxorubicin, procarbazine, Methotrexate and dacarbazine etc. in various types of cancer. In this review, we also discussed major structure modifications position of chemically synthesized vincristine and vinblastine derivatives required for potential anticancer activities. Anticancer mechanism and some major patents on vinca alkaloids derivatives also reported in this review article. The study of Vinca alkaloids and its derivatives a class of an antimitotic compounds, found essential role in anticancer therapies.

Post-valuation quality check of multiple-choice questions

Background: Multiple choice questions find important place in assessment in medical curriculum. Each MCQ is called item. Item analysis is quality check of MCQs after valuation of response sheets. This serves to recognize flaws in MCQs so that the given questions can be preserved in question bank, modified or deleted. Methods: 140 medical students of second MBBS were assessed pharmacology through 20 single best response type MCQ. Post exam validation of MCQs was done by item analysis. Each item was analyzed for level of difficulty, ability of the question to discriminate between poor and good performing students and distracter effectiveness.Results: Score ‘1’ was given for correct and ‘0’ for incorrect or no response. The mean score of the test was found to be 10.58±2.48, with a range 5-18. The whole test had an acceptable difficulty level with 43.25±17.81 mean difficulty index. The discrimination index of the whole test was found to be 0.123±0.184 mean PBI correlation coefficient, which is not satisfactory. Out of 60 distractors, 51 were found to be functional, hence distractor efficiency of the given test was 85%±19.96% which is acceptable.Conclusions: 60% questions were found to be ideal and 25% were acceptable with revision of stem or options. One question was easy with poor discrimination which needs major modification in construct or subject to be placed in question bank while another one question was difficult but had negative biserial index which needs to be deleted from the question bank.

Investigations on Caesium Dispersion and Molybdenum Coating on SPIDER Components

SPIDER is the 100 keV full-size Negative Ion Source prototype of the ITER Neutral Beam Injector, operating at Consorzio RFX in Padova, Italy. The largest Negative Ion Source in the world, SPIDER generates an RF driven plasma from which Deuterium or Hydrogen negative ions are produced and extracted. At the end of 2021, a scheduled long-term shutdown started to introduce major modifications and improvements aiming to solve issues and drawbacks identified during the first three years of SPIDER operations. The first action of the shutdown period was the disassembly and characterization of the SPIDER beam source after removal from the vacuum vessel and its placement inside the clean room. Each component was carefully assessed and catalogued, following a documented procedure. Some source components, i.e., the Plasma Grid, Extraction Grid and Bias Plate, revealed the presence of different and non-uniform red, white and green coatings that might be correlated to back-streaming positive ions impinging on grid surfaces, electrical discharges and caesium evaporation. Thus, several analyses have been carried out to understand the nature of such coatings, with the study still ongoing. The evidence of caesium evaporation and deposition on molybdenum-coated SPIDER components, such as the formation of oxides and hydroxides, is demonstrated through surface characterization analyses with the use of the Scanning Electron Microscope (SEM), X-ray Diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS).

Histone variants and modifications during abiotic stress response.

Plants have developed multiple mechanisms as an adaptive response to abiotic stresses, such as salinity, drought, heat, cold, and oxidative stress. Understanding these regulatory networks is critical for coping with the negative impact of abiotic stress on crop productivity worldwide and, eventually, for the rational design of strategies to improve plant performance. Plant alterations upon stress are driven by changes in transcriptional regulation, which rely on locus-specific changes in chromatin accessibility. This process encompasses post-translational modifications of histone proteins that alter the DNA-histones binding, the exchange of canonical histones by variants that modify chromatin conformation, and DNA methylation, which has an implication in the silencing and activation of hypervariable genes. Here, we review the current understanding of the role of the major epigenetic modifications during the abiotic stress response and discuss the intricate relationship among them.

Filling the gaps: missing taxon names at the ranks of class, order and family.

The International Code of Nomenclature of Prokaryotes (ICNP) recently underwent some major modifications regarding the higher taxonomic ranks. On the one hand, the phylum category was introduced into the ICNP, which rapidly led to the valid publication of more than forty names of phyla. On the other hand, a decision on the retroactivity of Rule 8 regarding the names of classes was made, which removed most of the nomenclatural uncertainty that had affected those names during the last decade. However, it turned out that a number of names at the ranks of class, order and family are either not validly published or are validly published but illegitimate, although these names occur in the literature and are based on the type genus of a phylum with a validly published name. A closer examination of the literature for these and similar cases indicates that the names are unavailable under the ICNP either because of minor formal errors in the original descriptions, because another name should have been adopted for the taxon when the name was proposed, because of taxonomic uncertainties that were settled in the meantime, or because the names were placed on the list of rejected names. The purpose of this article is to fill the gaps by providing the missing formal descriptions and to ensure that the resulting taxon names are attributed to the original authors who did the taxonomic work.

Crosstalk between Methylation and ncRNAs in Breast Cancer: Therapeutic and Diagnostic Implications

Breast cancer, as a highly heterogeneous malignant tumor, is one of the primary causes of death among females worldwide. The etiology of breast cancer involves aberrant epigenetic mechanisms and abnormal expression of certain non-coding RNA (ncRNAs). DNA methylation, N6-methyladenosine(m6A), and histone methylation are widely explored epigenetic regulation types in breast cancer. ncRNAs are a group of unique RNA transcripts, mainly including microRNA (miRNAs), long non-coding RNA (lncRNAs), circular RNA (circRNAs), small interfering RNA (siRNAs), piwi-interacting RNA (piRNAs), etc. Different types of methylation and ncRNAs mutually regulate and interact to form intricate networks to mediate precisely breast cancer genesis. In this review, we elaborate on the crosstalk between major methylation modifications and ncRNAs and discuss the role of their interaction in promoting breast cancer oncogenesis. This review can provide novel insights into establishing a new diagnostic marker system on methylation patterns of ncRNAs and therapeutic perspectives of combining ncRNA oligonucleotides and phytochemical drugs for breast cancer therapy.

A comparative assessment of operating characteristics of a diesel engine using 20% proportion of different biodiesel diesel blend

ABSTRACT The present work aims to find a viable substitute fuel for diesel and control pollutants from compression ignition engines. Therefore, in the present investigation, an attempt has been made to study the effect of 20% proportion of five different biodiesel diesel blends in diesel engine. The 20% proportion of biodiesel such as Jatropha, Pongamia, Mahua, Annona and Nerium and 80% of diesel is denoted as J20, P20, M20, A20 and N20 are used in the present investigation. The experimental results showed that the different biodiesel blends’ brake thermal efficiency is slightly lower compared to neat diesel fuel. However, the N20 blend has shown improvement in performance and reduction in exhaust emissions compared to other biodiesel diesel blends. From, the experimental work, it is found that biodiesel can be used up to 20% and 80% of a diesel engine without any major modification.

Optimising clinical care through CDH1-specific germline variant curation: improvement of clinical assertions and updated curation guidelines

BackgroundGermline pathogenic variants in CDH1 are associated with increased risk of diffuse gastric cancer and lobular breast cancer. Risk reduction strategies include consideration of prophylactic surgery, thereby making accurate interpretation...

Development of the expression and prognostic significance of m5 C-related LncRNAs in breast cancer.

5-Methylcytosine (m5C) methylation is a major epigenetic RNA modification and is closely related to tumorigenesis in various cancers. This study aimed to explore the prognostic value of m5C-related lncRNAs in breast cancer. Clinical characteristics and RNA-seq expression data from TCGA (The Cancer Genome Atlas) were used in the study. First, we performed differentially expressed gene (DEG) analysis and constructed a PPI network for the 12 m5C regulators. Then, we identified the m5C-related LncRNAs by the "cor. test." An m5C-related lncRNA prognostic risk signature was developed using univariate Cox regression and Lasso-penalized Cox regression analyses. The model's performance was determined using Kaplan-Meier (KM) survival analysis and ROC curves. Finally, a nomogram was constructed for clinical application in evaluating patients with BRCA. We also researched the drug sensitivity of signature lncRNAs and immune cell infiltration. Finally, we validated the expression of the signature lncRNAs through qRT-PCR in a breast cancer cell line and a breast epithelial cell line. Overall, we constructed an 11-lncRNA risk score signature based on the lncRNAs associated with m5C regulators. According to the median risk score, we divided BRCA patients into high- and low-risk groups. The prognostic risk signature displayed excellent accuracy and demonstrated sufficient independence from other clinical characteristics. The immune cell infiltration analysis showed that the prognostic risk signature was related to the infiltration of immune cell subtypes. Drug sensitivity proved that our prognostic risk signature potentially has therapeutic value. The m5C-related lncRNA signature reliably predicted the prognosis of breast cancer patients and may provide new insight into the breast cancer tumor immune microenvironment.

Review of Regulated, Unregulated and Particulate Emissions from Biodiesel Fuelled Compression Ignition Engines

Compression-ignition (CI) engines have been utilised in transport and power generation sectors due to their excellent thermal efficiency, robustness and durability. However, the rapid depletion of crude oil reserves and environmental concerns have forced CI engines to be powered by alternative and environment-friendly renewable fuels. Biodiesel, which contains ∼10 % (w/w) oxygen in its fuel molecules, has become a popular alternative fuel for CI engines. Biodiesel can be stored similar to mineral diesel. Hence it does not require a separate fuel distribution infrastructure. The fuel properties of biodiesel are very close to mineral diesel; hence conventional CI engines can be fueled with biodiesel/blends without major modifications in the engine hardware/software. It is also widely known that biodiesel in CI engines brings significant benefits of lower emissions such as unburned hydrocarbons, carbon monoxide, and particulate matter (PM). Since biodiesel is considered an attractive alternative fuel, there are many attempts to produce and utilise biodiesel in different applications from various feedstocks such as soybean, rapeseed, and waste cooking oil. Hence, it is necessary to understand the effects of biodiesel feedstocks and engine applications (operating conditions, engine type, etc.) on regulated and unregulated emissions. This paper reviews effects of biodiesel on regulated/unregulated/particulate matter (PM) emissions based on numerous experimental research studies in open literature conducted on a variety of engines using different biodiesels.

A steady state vs pulsed fusion neutron science facility

Two major modifications to the existing steady state fusion neutron science facility (FNSF) concept (Kessel et al 2018 Fusion Eng. Des. 135 236–70) are investigated with the aim of determining whether or not its predicted performance can be improved. The modifications are high magnetic field and pulsed operation. We find that high field leads to major economic improvements in a steady state FNSF, although at the expense of lowering the engineering gain. Pulsed operation replaces the problems associated with low current drive efficiency, with hopefully more manageable engineering problems. Here, however, high toroidal field (TF) is not helpful, and a lower TF field is more desirable economically. Pulsed FNSFs also have a reduced engineering gain. Further modifications lead to FNSF designs satisfying the additional constraint of engineering gain equal to unity. For these designs there is a large cost penalty for the steady state FNSF but only a modest penalty for the pulsed FNSF. All of our modified designs show modest to large potential economic improvements over the existing design. Overall, our conclusion is that it may be desirable to carry out a more detailed analysis of one of our improved designs, the choice depending upon which issue in the existing design is most important.

Alternative fuel production from waste plastics and their usability in light duty diesel engine: Combustion, energy, and environmental analysis

Green biofuels have long been touted as a potential solution to society's reliance on fossil fuels and pollution emission problems. Commercially available renewable fuels like waste plastics oil alternative (P) made from waste plastic oil could take the place of fossil fuels, especially in diesel engines. Binary alternatives and diesel blends that partially replace diesel can be used in diesel engines without requiring major modifications. A four-stroke diesel engine used in the experiment was fed waste plastics oil alternative (up to 40% volumetric content), and diesel (D) mixes. Each binary blend is stabilised by a volumetric concentration and is composed of varying oil proportions. The investigations, therefore, relate partial diesel replacements to assessments of engine performance and mix combustion under various load conditions (25–100%), speeds (1200–1800 rpm), and compression ratios (15–19). Results showed that alternative-diesel mixtures up to 40% can still operate reliably. Thermal efficiency was somewhat lower than for diesel which was 100%. The experiment revealed higher fuel use, smoke, and NO emissions. The conclusion of the present research states that waste product oils can compete with fossil fuels in terms of engine performance, combustion, and emission characteristics when utilized in light-duty engines.

Growth and Optical Properties of Ga2O3 Layers of Different Crystalline Modifications

In the present work, a new method of growing layers of three main crystal modifications of Ga2O3, namely α-phase, ε-phase, and β-phase, with thickness of 1 µm or more was developed. The method is based on the use of two approaches, namely a combination of Ga2O3 growth using the hydride vapor-phase epitaxy (HVPE) method and the use of a silicon crystal with a buffer layer of dislocation-free silicon carbide as a substrate. As a result, Ga2O3 gallium oxide layers of three major Ga2O3 crystal modifications were grown, namely, α-phase, ε-phase, and β-phase. The substrate temperatures and precursor flux values at which it is possible to grow only α-phase, only ε-phase, or only β-phase without a mixture of these phases were established. It was found that the metastable α- and ε-phases change into the stable β-phase when heated above 900 °C. Experimentally obtained Raman and ellipsometric spectra of α-phase, ε-phase, and β-phase of Ga2O3 are presented. The theoretical study of the Raman spectra and the dependences of dielectric function on photon energy for all three phases was carried out. The vibrations of Ga2O3 atoms corresponding to the main lines of the Raman spectrum of the α-phase, ε-phase, and β-phase were simulated by density functional methods.

Understanding home modification outcomes from the perspective of older people and people with a disability: informing home modification evaluation

Purpose There is limited evidence to guide therapists in home modification outcome evaluation. Involving consumers in evaluating home modifications is critical to progressing practice. This study investigated the home modification experience and outcomes most valued by older adults and individuals with a disability in Australia. Materials and methods An interpretive description approach was used. Twenty participants aged 24–93 who had received major home modifications were interviewed and transcripts were analysed thematically. Results Two major themes emerged: life before and during modifications – “it was all hard,” and life after modifications – “to have a life.” People’s prior experience of home, the circumstance of and time to plan modifications, the perceived value of the modifications, and the home modification process and funding influenced perceptions of outcomes. Valued home modification outcomes included those that enable people to experience emotional and physical health, and safety; do what they want; maintain the image of the home; and plan for the future. Experiences and perceptions of modifications ranged from supportive to disruptive. Conclusions These findings point to the need for therapists to deeply understand and evaluate their clients’ experiences of home and life as an initial and critical step in the home modification process. Implications for rehabilitation Major home modifications result in broad and meaningful outcomes of importance to clients. Occupational therapists need to partner with clients to understand their life before and during modifications to achieve positive outcomes. Evaluation of major home modifications needs to be integrated and prioritised in daily practice.

High Throughput Epigenomic Mapping of Hematopoietic Stem and Progenitor Cells Using Low Cell Input Acoustic Enhanced Immunoprecipitation

Acute myeloid leukemia (AML) is a complex disease with several etiologies characterized by incomplete cellular differentiation and clonal proliferation of hematopoietic stem and progenitor cells (HSPCs) in place of normal hematopoiesis. Non-mutational epigenetic reprogramming is an emerging hallmark and enabling characteristic of cancer pathogenesis. Epigenetic regulation of gene activity is an essential component of chromatin stability and maintenance of proper cellular function and differentiation. Modifications of gene and histone landscape methylation and acetylation, three-dimensional chromatin structure, and protein-controlled activating and silencing switches are all regulated by epigenetic components, and thus are integral to understanding gene regulatory mechanisms during cell fate changes, disease onset, progression, as well as for optimizing therapeutic regime. In addition, this information holds immense potential for accurate identification of disease biomarkers, cell of origin, and targets for epigenetic therapy. However, existing chromatin immunoprecipitation sequencing (ChIP-Seq) workflows require significant cellular material and are very time-consuming, requiring cumbersome optimization. Moreover, cellular heterogeneity can influence accuracy of results from the perspective of sample preparation efficiency and also population-biased effects. Both normal and malignant bone marrow are heterogeneous in cell type and stage of differentiation that leads to imprecise results with regard to epigenetic determinates. In our study, we implemented Covaris's Adaptive Focused Acoustics® (AFA®) technology to miniaturize sample volumes for both chromatin shearing and immunoprecipitation in a 96-well format. This approach enables a standardized, robust, automatable, half-day workflow for both ChIP and Hi-ChIP applications. AFA ChIPseq- was applied to flow sorted hematopoietic stem and progenitor cells (HSPC) from individual mice. We utilized a range of epitopes including major histone modifications (H3K9me1/2/3, H3K27ac) and factors (CTCF, cohesin). Interestingly, we found epitope-dependent differences in AFA-enhanced binding with CTCF saturating at 30 minutes, whereas H3K27ac required 4 hours of AFA pulse to facilitate chromatin binding. Additionally, we evaluated low input in vitro and in vivo samples, with limited dilution of lineage negative HSPCs ranging from 500,000 to 5,000 cells with comparable sheared chromatin yield and immunoprecipitated material. Overlap of peaks called by MACS2 was consistent among the various cell inputs as well as when compared with gold standard CTCF ChIPseq- data previously performed on pooled samples from our previously published work. The low cell input capability permitted deconvolution of dynamic epigenetic changes in protein binding and chromatin structure during normal lineage specification of hematopoietic stem cells. Epigenetic remodeling of these sites was consistent with established gene programs essential for myeloid differentiation. Data were found to be in alignment with standard ChIPseq- and Hi-C datasets generated from similar cell populations. The simplified and significantly shortened (Hi)ChIP-seq processes showcased in this study will be highly beneficial for both research and clinical diagnostic applications owing to their requirements for using 96-well plate format and their ability to become integrated into automated workflows. Taken together, this technology and workflow represents a high throughput epigenetic system for small cell numbers of cells with a multitude of applications enabling laboratories focused in cell biology and translational research.

EPCO-42. HYPO-TRIMETHYLATION OF HISTONE H3 LYSINE 4 AND HYPER-DI/TRIMETHYLATION OF HISTONE H3 LYSINE 27 AS THE EPIGENETIC MARKERS OF POOR PROGNOSIS FOR PRIMARY CENTRAL NERVOUS SYSTEM LYMPHOMA PATIENTS

Abstract PURPOSE This study aimed to investigate the methylation status of major histone modification sites in PCNSL samples and examine their prognostic role in PCNSL patients. METHODS Between 2007 and 2020, 87 patients were histopathologically diagnosed as PCNSL, and they were treated with same protocol of high-dose methotrexate-based combination chemotherapy. Clinical data for PFS and OS was obtained from medical records retrospectively. Immunohistochemical staining for FFPE samples of PCNSL from pathological archives in our institute was performed for major histone modification site such as histone H3K4, H3K9, H3K27, H3K14, and H3K36. After detection of meaningful methylation site, we examine the histone modification enzyme which induce methylation or demethylation on each site using immunohistochemical staining. The meaningful immunoreactivity was validated with qRT-PCR and western blotting using fresh tissue of PCNSL under refrigerated state in N2O container. RESULTS Among 87 patients, 28 patients (32.2%) experienced recurrence or progression and 11 patients (12.6%) succumbed their disease during follow-up for 12 months or more. More frequent recurrences was found in hypomethylation of H3K4me3 (p=0.004), and hypermethylation of H3K27me2 (p< 0.001) and H3K27me3 (p=0.002). These factors were also statistically related with short PFS and OS in univariate and multivariate analysis. At the next step, histone modification enzyme inducing demethylation of H3K4 (LSD1/2, KDM5A-D, NO66), and methylation of H3K27 (EZH1/2) was immunohistochemically stained. Among them, the immunoreactivity of KDM5A was reversely associated with methylation status of H3K4me3 (R2=-1.243), and immunoreactivity of EZH2 was directly associated with methylation status of H3K27me2 (R2=0.988) and H3K27me3 (R2=1.139). These results was confirmed using RT-PCR and western blotting in fresh sample of PCNSL. CONCLUSION Our presenting study suggest that hypomethylation of H3K4me3, and hypermethylation of H3K27me2 and H3K27me3 could be associated with poor outcome of PCNSL patients. These relationship was modified by KDM5A as H3K4 demethylase and EZH2 as H3K27 methyltransferase.

The role of RNA modification in the generation of acquired drug resistance in glioma

Glioma is the most common malignant tumor in the central nervous system. The clinical treatment strategy is mainly surgery combined with concurrent temozolomide chemotherapy, but patients can develop drug resistance during treatment, which severely limits its therapeutic efficacy. Epigenetic regulation at the RNA level is plastic and adaptable, and it can induce a variety of tumor responses to drugs. The regulators of RNA modification include methyltransferases, demethylases, and methylation binding proteins; these are also considered to play an important role in the development, prognosis, and therapeutic response of gliomas, which provides a basis for finding new targets of epigenetic drugs and resetting the sensitivity of tumor cells to temozolomide. This review discusses the relationship between the development of adaptive drug resistance and RNA modification in glioma and summarizes the progress of several major RNA modification strategies in this field, especially RNA m6A modification, m5C modification, and adenosine-to-inosine editing.

Sensitivity of structural and electronic properties of Li-ion battery cathode materials to Hubbard U correction: an efficient first-principle approach

A new material design approach using first-principles density functional theory (DFT) calculations with acceptable precision can elucidate many critical features in emerging high-performance lithium-ion battery fields. However, the dominant impact of transition metals (TM) as the major component and/or modification dopant element with localized d-electrons in this material system, which needs so-called Hubbard correction U, limits DFT to many extents. As the U correction is not completely transferable, one may need to recalculate it for the same input structure with structural or elemental changes. While the accurate calculation of U is costly, it is worth investigating its sensitivity to input system parameters, such as cell size, structure, and chemical composition, to dismiss unessential recalculations, especially in high-throughput schemes. Furthermore, implementing DFT + U to get the expected properties is computationally expensive too. The necessity of including the U correction for expected properties or its small variation should be investigated. In this study, Hubbard correction for TM using a density functional perturbation theory (DFPT) approach was considered to investigate the electronic structure and structural stability of LiNiO2, LiCoO2, and LiNi0.75Co0.167Al0.083O2. The effect of system parameters (cell size, structure, and chemical composition) on U values was considered for different systems. The dependency of calculated electronic densities of states, lattice parameters, preferred doping sites, and vacancy formation energy, as well as charge voltage, was investigated, and low and high sensitivities were discussed. Furthermore, the effect of different doping scenarios with Co and Al on structural stability was studied with the efficient DFT + U approach. The results showed that the structural variation due to doping and vacancy site formation has a negligible effect on calculated U values. Significant correlation of predicted electronic properties with U for all systems was observed, while calculated lattice parameters, as well as preferred doping sites, reflected very low dependency on U variations.