Major Livestock Species Research Articles

A comprehensive review of livestock development: insights into domestication, phylogenetics, diversity, and genomic advances.

Livestock plays an essential role in sustaining human livelihoods, offering a diverse range of species integral to food security, economic stability, and cultural traditions. The domestication of livestock, which began over 10,000years ago, has driven significant genetic changes in species such as cattle, buffaloes, sheep, goats, and pigs. Recent advancements in genomic technologies, including next-generation sequencing (NGS), genome-wide association studies (GWAS), and genomic selection, have dramatically enhanced our understanding of these genetic developments. This review brings together key research on the domestication process, phylogenetics, genetic diversity, and selection signatures within major livestock species. It emphasizes the importance of admixture studies and evolutionary forces like natural selection, genetic drift, and gene flow in shaping livestock populations. Additionally, the integration of machine learning with genomic data offers new perspectives on the functional roles of genes in adaptation and evolution. By exploring these genomic advancements, this review provides insights into genetic variation and evolutionary processes that could inform future approaches to improving livestock management and adaptation to environmental challenges, including climate change.

Potential approaches to create ultimate genotypes in crops and livestock.

Many thousands and, in some cases, millions of individuals from the major crop and livestock species have been genotyped and phenotyped for the purpose of genomic selection. 'Ultimate genotypes', in which the marker allele haplotypes with the most favorable effects on a target trait or traits in the population are combined together in silico, can be constructed from these datasets. Ultimate genotypes display up to six times the performance of the current best individuals in the population, as demonstrated for net profit in dairy cattle (incorporating a range of economic traits), yield in wheat and 100-seed weight in chickpea. However, current breeding strategies that aim to assemble ultimate genotypes through conventional crossing take many generations. As a hypothetical thought piece, here, we contemplate three future pathways for rapidly achieving ultimate genotypes: accelerated recombination with gene editing, direct editing of whole-genome haplotype sequences and synthetic biology.

Has genomic selection fulfilled its promise?

Abstract Genomic selection has been increasingly implemented since its initial description in 2001. The initial focus of activity was in the dairy industry in the developed world but it has now been adopted in all major livestock species, and adoption is underway in most agronomically important plants. The promise of genomic selection includes increased accuracy of estimation of genetic merit, earlier estimation of genetic merit, the capacity to make improvements in traits previously less tractable to genetic change, and the capacity to estimate genetic merit in animals without phenotypic records. Each of these benefits has been realised in practice, with the significance of the opportunity varying with species. At the same time, ease of adoption and realisation of benefits has varied, based on three broad parameters: the availability of suitable (and sufficiently cheap) genotyping tools, the prior extent of systematic recording of phenotypes, and the use of estimates of genetic merit in selection. The effectiveness of genomic selection depends on the existence and composition of a genomic reference population, and this is the most significant challenge for any industry, breed or breeding program seeking to achieve the available benefits. The challenge is two-fold: first, to ensure that sufficient of the right phenotypes are recorded on the right animals, and second, flowing directly from the first, is to fund the phenotyping effort – and challenges aspects are ongoing. “Right” in the context of both phenotypes and animals means most informative, or relevant to the breeding objective or goal. No standard approach has been outlined for addressing these challenges, but the review presents some simple conceptual thinking that can assist. Genomic selection also heightens the importance of ensuring maintenance of genetic diversity, and of monitoring changes in both selected and non-selected traits. Genomic selection has fulfilled its promise where applied effectively and is increasing scope for genetic improvement both in rate of progress and trait coverage. At the same time, its dependencies must be addressed in order to the potential benefits to be realised, and this will likely require new organisational and funding arrangements in many situations.

Application of Global Burden of Animal Diseases methods at country level: experiences of the Ethiopia case study.

Animals play a central role in human livelihoods and welfare. Animal diseases have a great impact on the benefits humans derive from animals and can also pose a risk to human health. Better control of animal diseases generates wider societal benefits, including reducing the climate and ecological impacts of livestock and improving animal welfare. To better understand the scale of investment justified for the control and prevention of animal disease, the wide-ranging impacts of disease on animal production and health must be measured. The Global Burden of Animal Diseases (GBADs) programme is quantifying animal disease burden from the local to global levels. The GBADs programme includes country case studies for national- and local-level analysis. Ethiopia is the first case study country in which GBADs methods have been applied. GBADs'Ethiopia case study consists of three activity areas: i) stakeholder engagement; ii) livestock disease burden estimation, including data collection, analytics, evidence generation and communication; and iii) capacity building in animal health economics. At the start of the case study, various stakeholder communication platforms were used to familiarise stakeholders with GBADs and engage their support in various ways, including data access, and, through this engagement, to ensure the programme tools and outputs were relevant and useful to their needs. Existing data were retrieved from multiple sources and used to estimate disease burden. This process involved multiple steps, including estimation of biomass and economic value, the Animal Health Loss Envelope (farm-level disease burden), wider economic impacts and attribution of the disease burden to different levels of causes. This was carried out for major livestock species (cattle, sheep, goats and poultry) in Ethiopia. Capacity building on animal health economics was carried out for GBADs end users to increase competence in utilising animal health economic evidence, including GBADs outputs. This article documents experiences of the implementation of these activities in the GBADs Ethiopia case study.

Phenotypic Description of the Native Cattle Found in Maharashtra State: A Review

India is rich in the world’s largest and most diverse livestock with about 535.78 million animals. Cattle is a major livestock species in India comprising nearly 36% of the total livestock population. According to 20th livestock census, Maharashtra comprises 13.99 million cattle population, out of which 9.38 million are indigenous and 4.60 million are exotic and crossbred cattle. Maharashtra is divided into nine agro-climatic zones on the basis of annual rainfall, soil type, vegetation and cropping pattern. Due to this climatic diversity, different cattle populations have been developed according to the local demand. Out of total 53 registered cattle breeds, 7 breeds viz., Dangi, Gaolao, Khillar, Red Kandhari, Deoni, Konkan Kapila and Kathani are found in Maharashtra. These breeds are generally draft type and can be used for transport and agriculture. Other cattle populations (nondescript) consist of nearly 67% of the total cattle of the state and Maharashtra ranks 5th position in total nondescript cattle population of India. Indigenous livestock is adapted to the adverse conditions of our country and is resistant to various diseases. Hence, there is an urgent need for identification, characterization, evaluation and documentation of the nondescript populations. Further, admixture level of these populations should be studied for developing suitable breeding and conservation strategies for the breeds and/ populations.

Provision of veterinary services in livestock holdings in the Russian Federation

Being the primary link in the disease notification system, specialists of the veterinary service in livestock holdings ensure continuous monitoring and control of animal health. This analysis includes assessment of the actual availability of veterinary services in the absolute majority of animal holdings in 85 Subjects of the Russian Federation. In total, the study covered 6,226,368 holdings for major livestock species, such as cattle and small ruminants, pigs, poultry, horses and fur animals. Small-scale holdings have been shown to account for the largest proportion (99.7%) of the total number of livestock farms, while the proportion of holdings where animal health control is daily organized has varied from 0.03% in fur farms to 3% in poultry farms. The significant role of animal owners in small-scale holdings within the implementation of epizootological surveillance has been determined. It was revealed that the main populations of pigs, poultry and fur animals are concentrated in large-scale livestock farms. The study results indicate a relatively favorable situation in pig and poultry holdings, where only single cases of lack of veterinary service were reported. On the contrary there are multiple cases of lack of veterinary care in the farms for rearing cattle, small ruminants, fur animals. The paper highlights the mechanisms for the implementation of tasks assigned to the state veterinary service in terms of the organization of planned preventive and diagnostic veterinary measures in large pig breeding establishments. Proposals were made to introduce a legally fixed obligation for animal owners in large-scale livestock holdings to establish and maintain the production veterinary service, as well as a proposal to establish divisions of the state veterinary service in large-scale livestock establishments.

PSV-21 Evaluation of the Effect of Daily Probiotic Administration on the Prevalence of Shiga Toxigenic E.Coli and Select Serum and Immune Parameters During Pre and Peri-Weaning Period in Goat Kids

Abstract Probiotics are microorganisms generally regarded as safe (GRAS) that have a beneficial effect when ingested by a host. While research on the benefits of probiotics in farm animals has mainly concentrated on major livestock species, little is documented on potential benefits in small ruminants. Probiotics in goats and sheep could offer a dual benefit of reducing pathogenic bacteria loads and resulting in improved immunity and overall health. In this study, we evaluated the effect of one-month daily commercial probiotic administration followed by weekly administration until weaning and daily administration for one week after weaning on select serum parameters, and also the prevalence of pathogenic E.coli in healthy pre-weaned goat kids beginning at two days of age. Overall, the general health of the animals in both groups remained the same throughout the study period. No significant difference in total lactic acid bacteria was detected between probiotic supplemented and control group. Probiotic administration generally improved the total serum protein and immune markers. The difference was significant after twenty-eight days of administration and two days after weaning for total serum protein and immune markers, respectively. Shiga toxin E.coli (STEC) was detected in both control and probiotic-treated goats in early life, but no significant differences were detected between treatment groups on prevalence. These results suggest that daily probiotic administration may have some health benefits in pre-weaned goats.

Analysis of commercial fetal bovine serum (FBS) and its substitutes in the development of cultured meat

Fetal bovine serum (FBS) is an extremely important culture growth supplement, accounting for approximately 60 % of cell-culture-media costs; therefore, lowering FBS-acquisition costs for the industrialization of cultured meat is imperative. This study attempted to produce an FBS substitute using discarded livestock by-products, with particular focus on formulating a product with a composition similar to that of FBS to improve effectiveness. However, to date, no study has precisely analyzed the commercial components of FBS, and this study is the first to compare the chemical composition of FBS and commercially available horse serum purchased from the United States or Europe with that of FBS substitutes developed by our team. This study analyzed the chemical composition of the FBS products purchased by our team over the past 3 years via blood, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and independent composition analyses. While the composition and quality of commercial FBS products are known to vary, the FBS composition of our purchased products was relatively uniform regardless of company, brand, or country of origin. In contrast, FBS substitutes obtained from three major livestock species (cattle, pig, and chicken) clearly exhibited differences in composition, a phenomenon that was also observed upon comparing with FBS as well as among different species. Therefore, to replace commercial FBS entirely, the production of a proportionately effective substitute product comprising an equal or similar composition is required, and the results of this study can be a steppingstone to achieving this. In addition, FBS substitutes manufactured using inexpensive slaughter by-products as raw materials are expected to ultimately reduce the unit cost of cultured meat production.

Seroprevalence of <i>Brucella</i> infection in cattle and small ruminants in South Omo zone, southern Ethiopia

A cross-sectional study was conducted in selected districts of the South Omo Zone to estimate the seroprevalence of brucellosis and its associated risk factors. Additionally, the knowledge, attitude, and practices (KAP) of livestock keepers about the disease were also assessed. A total of 1349 sera samples were collected from 450 cattle and 899 small ruminants (450 goats and 449 sheep) kept under an extensive production system. Rose Bengal Plate Test was used for screening and ELISA as a confirmatory test for the detection of antibodies against Brucella species. Based on confirmatory tests, the overall seroprevalence of brucellosis was 2.2 % (95% CI: 1.1 – 4.1%) in cattle, 2.0% (95% CI: 0.9, 3.8%) in goats and 1.3% (95% CI: 0.5, 2.9%) in sheep with higher seropositivity in cattle compared to small ruminants. Seropositivity did not vary significantly (p> 0.05) with agroecology, age, and sex groups in cattle. However, a higher seroprevalence of 2.7% was detected in male cattle compared to 1.6% for females. Seroprevalence was higher in small ruminants from the lowland agroecology (3.0%) than those from the midlands (0.8%). Study respondents had a low level of knowledge on brucellosis with only 43% of them having some information about the disease. Most of the respondents have routinely engaged in practices that can expose them to infections such as assisted delivery (65%), contact with after-birth materials (50%), and handling aborted materials (15%) without any protection. High consumption of unpasteurized dairy products (93%) such as raw whole milk and traditionally fermented dairy product is also common. This study provides information on the occurrence f brucellosis in major livestock species kept at cross-border marginal areas with limited disease information. The existence of various exposure practices implies the need for creating awareness among livestock keepers on general disease transmission and its zoonotic role.

Partipatory disease surveilance in Fentale & Adama Districts of East Shewa Zone, Oromia Regional State

Livestock play a major role in the livelihoods of over 70% of the population. Small ruminants provide with a vast range of products and services, including milk, meat, skins, and wool throughout the year. Diseases of small ruminants are one of the common problems that hinder production. One is Peste des petits ruminants (PPR), or small ruminant plague, is a viral disease primarily affecting goats and sheep. The objectives of the study were to prioritize the major diseases of sheep and goat in selected districts of oromia Regional state based on producer’s perception in participatory manner and to understand the status of PPR through PDS technique to enlighten further epidemiological study and future control endeavor. Two districts were selected from the zone (Fentale and Adama) and the selection of the PAs (the lowest administrative level in Ethiopia) in each district was determined in discussion with the district livestock offices. A PDS team from Asella Regional Veterinary Laboratory was deployed to field and collected, reviewed of secondary data and participatory disease search at the zone. Participatory epidemiology techniques like Semi structured interviewing, Ranking and scoring, participatory mapping, proportional pilling and semi-structured interview and seasonal disease calendars were utilized. The major livestock species raised in Fentale are cattle, Sheep, goats, horses, donkeys, camels and poultry and whereas, major livestock species rose in Adama are cattle, Goat, Sheep, donkeys, Horse, mules and poultry. In all district, disease is mentioned the major challenge to the production of small ruminant. A disease like PPR, Fasciolosis, Pasterolosis, footrot, Sheep and Goat pox were the main small ruminant disease mentioned.

The role of goats in the world: Society, science, and sustainability

The role of goats in nutrition, health, and food security is underscored by the continuous increase in global production and consumption of goat milk and meat. The goat sector is diversified as people from a wider geographical distribution area are consuming milk and meat from goats. The goat industry will continue to experience structural changes that will impact society, the economy, and the environment. Production efficiency and global per capita consumption of goat milk and meat, however, lag behind some major livestock species. Scientific and technological developments in nutrition, genetics, reproduction, health and management remain critical for a viable goat sector in the future. The global dichotomy of goat production systems, from subsistence to industrialized, present a challenge of a uniformed strategy and direction for sustainable production. Animal welfare, environmental preservation, product safety, and health benefits are important considerations in consuming and producing organic goat products. Goat production can be part of the solution for the assuagement of food insecurity and malnutrition. Leverage biological, metabolic, behavioral, social and economic advantages of goats can alleviate poverty in the most vulnerable regions in the world.

Serological and molecular prevalence of Brucella spp. among livestock species in Rajasthan, India.

A seroprevalence and molecular study was carried out in six districts of the state of Rajasthan, India to detect brucellosis in major livestock species. This study involves the testing of 3,245 livestock samples using the Rose Bengal Plate Test (RBPT), Indirect Enzyme-Linked Immunosorbent Assay (i-ELISA), and genus-specific polymerase chain reaction (PCR) markers for molecular diagnosis of the disease. In the tested samples, seroprevalence was 5.06% (CI: 1.96-8.15) using the RBPT test and 6.88% (CI: 1.98-11.78) using the i-ELISA test, while the cumulative seroprevalence (RBPT and i-ELISA) was 3.63% (CI: 0.44-6.83). The prevalence of the disease was 1.27% (CI: 0.56-3.11) when tested using molecular markers. The highest prevalence of brucellosis was detected in Cattle (7.00, 3.22%), followed by camels (5.50, 2.50%), buffalo (2.66, 0.00%), sheep (2.43, 0.41%), and goats (0.58, 0.23%) when serological (cumulative) and molecular diagnosis were considered preferred methods of detection. Cattle (3.22%) and camels (2.50%) also showed a high prevalence of disease when tested using molecular markers. The results of this study reveal that cattle, camel, and sheep brucellosis is prevalent in the study areas.

Extracting functionally accurate context-specific models of Atlantic salmon metabolism

Constraint-based models (CBMs) are used to study metabolic network structure and function in organisms ranging from microbes to multicellular eukaryotes. Published CBMs are usually generic rather than context-specific, meaning that they do not capture differences in reaction activities, which, in turn, determine metabolic capabilities, between cell types, tissues, environments, or other conditions. Only a subset of a CBM’s metabolic reactions and capabilities are likely to be active in any given context, and several methods have therefore been developed to extract context-specific models from generic CBMs through integration of omics data. We tested the ability of six model extraction methods (MEMs) to create functionally accurate context-specific models of Atlantic salmon using a generic CBM (SALARECON) and liver transcriptomics data from contexts differing in water salinity (life stage) and dietary lipids. Three MEMs (iMAT, INIT, and GIMME) outperformed the others in terms of functional accuracy, which we defined as the extracted models’ ability to perform context-specific metabolic tasks inferred directly from the data, and one MEM (GIMME) was faster than the others. Context-specific versions of SALARECON consistently outperformed the generic version, showing that context-specific modeling better captures salmon metabolism. Thus, we demonstrate that results from human studies also hold for a non-mammalian animal and major livestock species.

염소와 양 골격근의 근섬유 특성

This study aimed to provide a fundamental summary of the caprine and ovine muscle fiber characteristics. The goat and sheep industries have been experiencing growth due to their adaptability to market changes and the diversity of their products. Muscle fibers, which represent the basic unit of muscle and an essential factor in assessing meat quality, can be classified into several types by their contractile and metabolic properties. Species, breeds, muscle type, age, and sex are the main factors affecting muscle fiber characteristics. While the effects of these factors on muscle fiber characteristics of major livestock species (cattle, pigs, and chickens) are widely studied, goats and sheep (the main meat sources among minor livestock species) have not fully studied. Understanding muscle fiber characteristics will help to development technologies for regulating goat and lamb meat quality. In the present study, the muscle fiber characteristics of the caprine and ovine skeletal muscles assessed in previous studies were summarized. In addition, the effects of breed, sex, age, and muscle type on the caprine and ovine muscle fiber characteristics were assessed using the results of previous studies. Specific in-depth studies on muscle fiber characteristics and their relationship to the quality characteristics of goat and lamb meat are required. These studies will help to extend shelf-life and retard deterioration in meat quality during storage or freeze-thawing of goat and lamb meat.

Metabolomics-Based Analysis of the Major Taste Contributors of Meat by Comparing Differences in Muscle Tissue between Chickens and Common Livestock Species.

The taste of meat is the result of complex chemical reactions. In this study, non-target metabolomics was used to resolve the taste differences in muscle tissue of four major livestock species (chicken, duck, pork, and beef). The electronic tongue was then combined to identify the major taste contributors to meat. The results showed that the metabolism of chicken meat differed from that of duck, pork, and beef. The multivariate statistical analysis showed that the five important metabolites responsible for the differences were all related to taste, including creatinine, hypoxanthine, gamma-aminobutyric acid, L-glutamic acid, and L-aspartic acid. These five key taste contributors acted mainly through the amino acid metabolic pathways. In combination with electronic tongue (e-tongue) analysis, inosine monophosphate was the main contributor of umami. L-Glutamic acid and L-aspartic acid might be important contributors to the umami richness. Creatinine and hypoxanthine contributed more to the bitter aftertaste of meat.

Summer grazing by three livestock species at moderate intensity enhances primary productivity and drives community divergence in a semi‐arid steppe

AbstractAimsThe general effects of grazing by large herbivores on plant species composition and productivity are well known. Meanwhile, how different livestock species affect community structure is not fully understood. In northern China, livestock species composition is changing rapidly and hence, it is imperative to understand the resulting effects on grassland productivity and composition.LocationXilingol, Inner Mongolia, China.MethodsWe studied how grazing by three major livestock species affects typical steppe dominated by Leymus chinensis, Stipa grandis, Cleistogenes squarrosa and Carex korshinskyi, in a 4‐year field experiment using a random block‐design with four treatments (sheep, goat, cattle, no grazing), under summer grazing at moderate intensity. We calculated above‐ground net primary productivity (ANPP) and a selectivity index (SI) based on biomass to quantify livestock forage preferences.ResultWe found that (i) sheep preferred Leymus chinensis and mixed subordinate species, cattle preferred Stipa grandis, and goat preferred Carex korshinskyi and Cleistogenes squarrosa. (ii) Consistent with these preferences, sheep increased the relative biomass of Stipa grandis, but reduced that of Carex korshinskyi; cattle reduced the relative biomass of Stipa grandis and Carex korshinskyi but increased that of mixed subordinate species; and goats reduced the relative biomass of Cleistogenes squarrosa and Carex korshinskyi but increased that of mixed subordinate species. (iii) Overall, grazing significantly increased ANPP, mainly due to an increase in ANPP of Leymus chinensis and subordinate species, at the cost of Stipa grandis.ConclusionsSummer grazing by different livestock species at moderate intensity can increase ANPP, while livestock forage preferences can drive divergent changes in species composition. Our results also imply that livestock composition can be used as a mean to manage vegetation dynamics.

Complete Genome Sequence of Four Strains of Leptospira borgpetersenii serovar Hardjo isolated from Cattle in the Central United States.

Pathogenic species of Leptospira cause leptospirosis, a global zoonotic disease affecting humans and all major livestock species. Cattle act as a reservoir host for L. borgpetersenii serovar Hardjo which colonize the kidneys and reproductive tract from which they are excreted and transmitted to other cattle via urine, semen or uterine discharges. Bovine leptospirosis results in reproductive failure, abortion, stillbirth and loss of milk production, and is an occupational risk for those working with infected animals. A recent study determined that 7.2% of cattle from an abattoir in the central United States were actively shedding pathogenic Leptospira. Here, we report and compare the complete genome sequence of four recent isolates of L. borgpetersenii serovar Hardjo designated strain TC112, TC147, TC129, and TC273.

From the Eurasian Steppes to the Roman Circuses: A Review of Early Development of Horse Breeding and Management.

Simple SummaryHorses were domesticated later than any other major livestock species. Their role in shaping ancient civilizations cannot be overestimated. As a primary means of transportation, an essential asset in warfare, and later one of the key elements of circus entertainment, horses quickly became luxurious goods. Vast amounts of money were invested in the horse industry resulted resulting in the rapid development of horse breeding and husbandry. This review examines paleogenetic, archeological, and classical studies on managing horses in antiquity. Many ancient approaches and practices in horse management are still relevant today and some of them, now abandoned, are worth re-examination.The domestication of the horse began about 5500 years ago in the Eurasian steppes. In the following millennia horses spread across the ancient world, and their role in transportation and warfare affected every ancient culture. Ownership of horses became an indicator of wealth and social status. The importance of horses led to a growing interest in their breeding and management. Many phenotypic traits, such as height, behavior, and speed potential, have been proven to be a subject of selection; however, the details of ancient breeding practices remain mostly unknown. From the fourth millennium BP, through the Iron Age, many literature sources thoroughly describe horse training systems, as well as various aspects of husbandry, many of which are still in use today. The striking resemblance of ancient and modern equine practices leaves us wondering how much was accomplished through four thousand years of horse breeding.

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea.

Background and Aim:Goat is one of the major livestock species, plays an important role in the economy of Bangladesh. However, the outbreak of different infectious diseases in goats causes high mortality and economic losses due to lack of proper diagnosis and treatment. Conventional culture-based methods for detecting specific pathogens as confirmatory diagnosis are laborious as well as time-consuming in comparison to multiplex polymerase chain reaction (mPCR), by which multiple pathogens can be detected at a time. The present study was aimed to perform faster molecular identification of bacterial pathogens from goats presumed with fever and/or diarrhea and their antimicrobial resistance (AMR) pattern.Materials and Methods:A total of 200 blood samples were collected from goats at S. A. Quaderi Teaching Veterinary Hospital (SAQTVH) in Chattogram Veterinary and Animal Sciences University for the period of July 2017-April 2018. DNA was extracted and subsequently, mPCR assay was performed for the screening of several bacterial pathogens (Salmonella spp., Listeria monocytogenes, Bacillus cereus, Yersinia enterocolitica, Campylobacter jejuni, Campylobacter coli, Clostridium perfringens, Vibrio cholerae, and Staphylococcus aureus). An antimicrobial susceptibility test against ten antimicrobials for positive samples of each organism was conducted using the Kirby–Bauer Disk-Diffusion Method on selective media.Results:S. aureus, C. perfringens, L. monocytogenes, and Salmonella spp. were detected from collected samples and their overall prevalence was 11.5%, 3.5%, 1%, and 20.5%, respectively. The most common clinical signs were mild fever, nasal discharge, dyspnea, and coughing (39.1%) for S. aureus, diarrhea, convulsion, abdominal pain, and incoordination (57.1%) for C. perfringens, fever, protrusion of tongue, and incoordination (100%) for L. monocytogenes, and fever, anorexia, dehydration with mucous feces (36.6%) for Salmonella spp. infection in goats. AntimGentamicinicrobial diagram of S. aureus showed resistance against Cefotaxime (74%), Cefixime (65%), and Tetracycline (65%); highly sensitive against Amoxicillin (48%), Ciprofloxacin (44%), and Gentamicin (44%). On the other hand, C. perfringens showed highly resistant against Ampicillin (71%), Gentamicin (71%), sensitive against Penicillin (57%), and Cefotaxime (57%). L. monocytogenes were found to be sensitive to Penicillin (100%) and Cefixime (100%) and Salmonella spp. showed resistance to Ampicillin (78%) and Amoxicillin (59%) but sensitive to Ciprofloxacin (54%).Conclusion:This study identified pathogens with their specific clinical signs in goats presumed fever and/or diarrhea through mPCR with their AMR pattern in SAQTVH, Chattogram. Potential risk factors, measuring the strength of association of disease caused by these particular pathogens, were also determined. mPCR may use as an effective tool for rapid detection of pathogens in animal.

SARS-CoV-2 in animals: From potential hosts to animal models.

Within only one year after the first detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), nearly 100 million infections were reported in the human population globally, with more than two million fatal cases. While SARS-CoV-2 most likely originated from a natural wildlife reservoir, neither the immediate viral precursor nor the reservoir or intermediate hosts have been identified conclusively. Due to its zoonotic origin, SARS-CoV-2 may also be relevant to animals. Thus, to evaluate the host range of the virus and to assess the risk to act as potential animal reservoir, a large number of different animal species were experimentally infected with SARS-CoV-2 or monitored in the field in the last months. In this review, we provide an update on studies describing permissive and resistant animal species. Using a scoring system based on viral genome detection subsequent to SARS-CoV-2 inoculation, seroconversion, the development of clinical signs and transmission to conspecifics or humans, the susceptibility of diverse animal species was classified on a semi-quantitative scale. While major livestock species such as pigs, cattle and poultry are mostly resistant, companion animals appear moderately susceptible, while several model animal species used in research, including several Cricetidae species and non-human primates, are highly susceptible to SARS-CoV-2 infection. By natural infections, it became obvious that American minks (Neovison vison) in fur farms, e.g., in the Netherlands and Denmark are highly susceptible resulting in local epidemics in these animals.