Major Staple Food Research Articles

English

Foods rich in amylose and resistant starch (RS) have great potential to improve human health and lower the risk of noninfectious diseases. Common wheat (Triticum aestivumL.) is a major staple food crop with low RS content in the grains. The content of RS, preferentially derived from amylose, may be increased by suppressing amylopectin synthesis viasilencing the starch branching enzyme (SBE) II a or/and starch synthase (SS)IIa. In this study, SBEIIaand SSIIawere silenced separately and simultaneously using a barley stripe mosaic virus-virus-induced gene silencing (BSMV-VIGS) system. Compared with grains from control BSMV:00-inoculated spikes, grains from BSMV:SBEIIa-and BSMV:SSIIa-infected spikeshad fewer SBEIIa and SSIIa transcripts,together with increased amylose contents (18.62 and 24.48%, respectively) and RS contents (11.61 and 16.67%, respectively). Infection with BSMV:SBEIIa-SSIIa reduced SBEIIa and SSIIatranscript levels and increased the amylose and RS contents (32.02 and 22.33%, respectively). Thus, BSMV-VIGS is a useful tool for the rapid silencing of single or multiple starch synthase-related genes and BSMV shows great potential to study the functions of genes involved in starch biosynthesis or other processes/traits in developing grains.We showed that the SSIIagene plays an important role in the synthesis of amylose and RS and that the effects of simultaneously silencing SBEIIaand SSIIaon starch synthesis aregreater than those of single gene silencing. Our study lays the foundation for the molecular design-based breeding of high-amylose and high-RS wheat.© 2021 Friends Science Publishers

Detection and diversity of viruses infecting African yam (Dioscorea rotundata) in a collection and F1 progenies in Côte d'Ivoire shed light to plant-to-plant viral transmission.

Yam (Dioscorea spp.) is a major staple food whose production is hampered by viral diseases. However, the prevalence, diversity, transmission, and impact of yam‐infecting viruses remain poorly documented. This study reports on the symptomatology, prevalence, and molecular diversity of eight viruses in 38 D. rotundata accessions from a germplasm collection and 206 F1 hybrid progenies maintained in Côte d'Ivoire. Mean severity scores as assessed from leaf symptoms ranged from 2 to 4 in the germplasm collection and from 1 to 3 in F1 hybrids, respectively. Dioscorea mosaic‐associated virus (DMaV), potexviruses, and yam mosaic virus (YMV) were detected by PCR‐based diagnosis tools in single and mixed infections in both the D. rotundata collection and F1 progenies, whereas badnaviruses were detected only in the germplasm collection. In contrast, cucumber mosaic virus (CMV), yam macluraviruses, yam asymptomatic virus 1 (YaV1), and yam mild mosaic virus (YMMV) could not be detected. No correlation could be established between severity scores and indexing results. Phylogenetic analysis performed on partial viral sequences amplified from infected samples unveiled the presence of two putative novel viral species belonging to genera Badnavirus and Potexvirus and provided evidence for plant‐to‐plant transmission of YMV, DMaV, and yam potexviruses.

Assessing the impacts of climatic and technological factors on rice production: Empirical evidence from Nepal

Considering the significant impact of climate change on major staple food crops in Nepal, this study aims to estimate the influence of climatic factors (i.e., CO2 emissions, average temperature, and average precipitation) and technological factors (i.e., fertiliser consumption and improved seeds) on rice production in Nepal from 1990 to 2016 using the autoregressive distributed lag (ARDL) approach. While controlling for cultivated rice areas, agricultural credit variables are likely other essential rice production factors. This study reveals a long-run cointegration connection among the variables. The ARDL results indicate that CO2 emissions decreased rice production by 0.13%, while average temperature and average precipitation improved rice production by 0.72% and 0.01%, respectively, in the long run. Further results show that cultivated rice area, fertiliser consumption, and agricultural credit enhanced rice production by 2.26%, 0.05%, and 0.02%, respectively, in the long run. Unidirectional causality among cultivated areas, fertilisers, seeds, temperature, CO2 emissions, and rice production was confirmed. Additionally, impulse response and variance decomposition verified the substantial impacts of climate and technological factors on rice production and variations. This study empirically confirmed that the use of agricultural technology (i.e. fertiliser consumption) significantly enhanced rice production; therefore, this study suggests that the Nepalese government should expand subsidised fertilisers so as to increase rice production and improve the income of farmers. In addition, agricultural credit plays a vital role in enhancing rice production in Nepal; to cope with climate change, the study also suggests that there is a need to launch carbon/weather financing schemes through financial intuitions in the country.

Simulating changes in soil carbon stocks for Quercus forests and upland rice fields under climate change scenarios in Manipur (India)

Assessing soil organic carbon (SOC) stocks under different land uses and future climate change (CC) conditions is important to provide better land use planning strategies. In South and Southeast Asia, Quercus forests are widely distributed but are exploited as a source of raw materials and shifting cultivation. Rice is a major staple food grain crop, often cultivated with terracing as upland rice. However, few studies on SOC stocks and simulated SOC dynamics under climate change conditions are available in these regions for these important land uses. Here, we present a case study related to Manipur state in North East region (NER) of India. We used the RothC model to evaluate the short-term changes of SOC stocks in Quercus forests and nearby upland rice fields and the effects of CC on SOC stocks, based on two General Circulation Models (GCMs) and two CO2 Representative Concentration Pathways (RCPs) during three 20-year time periods. Under CC conditions (2019–2078), results differed among land uses, GCMs, RCPs, and time periods, and Quercus forests were more sensitive to climate change than upland rice. For Quercus forests, as average of all GCMs and RCPs, SOC decreased by 0.42 t C ha−1 year−1 in the first time period and by 0.17 t C ha−1 year−1 as sixty years average. In upland rice, SOC decreased by 0.20 t C ha−1 year−1 in the first time period and by 0.12 t C ha−1 year−1 as sixty years average. Results indicated that management strategies for Quercus forests and upland rice are needed for CC mitigation and adaptation particularly in very close CC scenarios.

Economics of Organic vs Inorganic Rice Production: A Case of Chitwan District of Nepal


 
 
 
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 Rice (Oryza sativa L.) is the major staple food crop of Nepal. A research was conducted in December, 2019 to examine the economic viability of organic rice farming and factors affecting its adoption. Fulbari area of Chitwan district of Nepal was purposively selected for this study on consultation with Agriculture Knowledge Centre, Chitwan and review of past works which showed good practice of organic rice farming. A total of 100 farm households, 50 organic and 50 inorganic were selected using simple random sampling. Primary data were collected through a pre-tested semi-structured interview schedule, while secondary data were collected from related publications. The probit regression analysis showed that the six variables were statistically significant for the decision to adopt organic rice farming, they are: number of schooling years of the household head (p= 0.014), annual household income from agriculture (p= 0.012), membership of any agricultural organization (p= 0.000), subsidy in other inputs in addition to seed and fertilizers (p= 0.000), area of rice cultivated land (p= 0.007) and awareness on climate change (0.086). The benefit cost ratio of organic rice production (2.2) was higher as compared to inorganic (1.9) which showed that the profitability in organic rice production was higher than in inorganic. Furthermore, indexing identified- lengthy certification process (0.85) as the first major hindrance in adoption of organic rice farming followed by lack of proper organic market (0.68), high cost of organic seed and fertilizer (0.56), lack of knowledge and trainings on organic farming (0.48) and lack of government support such as grants and premium price (0.44) as the second, third, fourth and fifth hindrances respectively. The government should promote organic rice farming, prioritizing the small scale and marginal farmers by giving subsidies and premium, facilitating in certification and marketing, and providing knowledge about climate change.

Land Cover Changes in Ghana over the Past 24 Years

Changes in land cover (LC) can lead to environmental challenges, but few studies have investigated LC changes at a country wide scale in Ghana. Tracking LC changes at such a scale overtime is relevant for devising solutions to emerging issues. This study examined LC changes in Ghana for the past almost two and half decades covering 1995–2019 to highlight significant changes and opportunities for sustainable development. The study used land cover data for six selected years (1995, 2000, 2005, 2010, 2015, and 2019) obtained from the European Space Agency. The data was analyzed using R, ArcGIS Pro and Microsoft Excel 365 ProPlus. The original data was reclassified into eight LC categories, namely: agriculture, bare area, built-up, forest, grassland, other vegetation, waterbody, and wetland. On average, the results revealed 0.7%, 131.7%, 23.3%, 46.9%, and 11.2% increases for agriculture, built-up, forest, waterbody, and wetland, respectively, across the nation. However, losses were observed for bare area (92.8%), grassland (51.1%), and other vegetation (41%) LCs overall. Notably, agricultural land use increased up to 2015 and decreased subsequently but this did not affect production of the major staple foods. These findings reveal the importance of LC monitoring and the need for strategic efforts to address the causes of undesirable change.

A world-wide analysis of reduced sensitivity to DMI fungicides in the banana pathogen Pseudocercospora fijiensis.

BACKGROUND Pseudocercospora fijiensis is the causal agent of the black leaf streak disease (BLSD) of banana. Bananas are important global export commodities and a major staple food. Their susceptibility to BLSD pushes disease management towards excessive fungicide use, largely relying on multisite inhibitors and sterol demethylation inhibitors (DMIs). These fungicides are ubiquitous in plant disease control, targeting the CYP51 enzyme. We examined sensitivity to DMIs in P. fijiensis field isolates collected from various major banana production zones in Colombia, Costa Rica, Dominican Republic, Ecuador, the Philippines, Guadalupe, Martinique and Cameroon and determined the underlying genetic reasons for the observed phenotypes.RESULTSWe observed a continuous range of sensitivity towards the DMI fungicides difenoconazole, epoxiconazole and propiconazole with clear cross‐sensitivity. Sequence analyses of PfCYP51 in 266 isolates showed 28 independent amino acid substitutions, nine of which correlated with reduced sensitivity to DMIs. In addition to the mutations, we observed up to six insertions in the Pfcyp51 promoter. Such promoter insertions contain repeated elements with a palindromic core and correlate with the enhanced expression of Pfcyp51 and hence with reduced DMI sensitivity. Wild‐type isolates from unsprayed bananas fields did not contain any promoter insertions.CONCLUSIONThe presented data significantly contribute to understanding of the evolution and global distribution of DMI resistance mechanisms in P. fijiensis field populations and facilitate the prediction of different DMI efficacy. The overall reduced DMI sensitivity calls for the deployment of a wider range of solutions for sustainable control of this major banana disease. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Development of Irrigation Regime of Limited and Unlimited Water Supply for Satisfactory Rice Yield

Rice is the staple food for majority of the population worldwide and is a water-consuming crop. Decreasing water availability due to climate change necessitates the development of water saving rice production technology. The objective of this study was to develop water management strategies for two contrast scenarios of water availability for improvement of rice yield with high water productivity. A pot experiment using different levels of water percentages for water management treatments in rice was conducted inside a greenhouse during the wet

Impacts of climate change and increasing carbon dioxide levels on yield changes of major crops in suitable planting areas in China by the 2050s

Maize, rice, and wheat are the major staple food crops in China and are crucial components of national food security and economic development. The cultivation and production of these crops are expected to be affected by climate change and elevated atmospheric carbon dioxide (CO2) concentration, and have drawn considerable public attention. The objective of this experiment was to understand the impact of future climate change (including increased temperature and changed precipitation patterns) and elevated CO2 concentration on variations of crop yields in their suitable planting areas. We conducted a spatial grid-based analysis of maize, rice, and wheat yields using projections of future climate generated by a multi-model ensemble of global climate models for three representative concentration pathway scenarios (RCP2.6, RCP4.5, and RCP8.5) in suitable planting areas in China for the 2030s (2021–2040) and the 2050s (2041–2060). Suitable areas for the planting of maize, rice, and wheat under the high-emission scenarios migrated slightly northward over time. Yield of all three crops would be expected to remain stable or to slightly increase across China in the future. A possible reason for this result may be because the positive effects of increased precipitation and CO2 offset the negative effect of increased temperature on crop yields, resulting in a much more appropriate growth environment and increased biomass accumulation and crop yield. In addition, this study also indicated that changes in crop yields were mainly driven by temperature and CO2 factors. The potential effects of climate change and elevated CO2 concentration on migration of planting areas and yield fluctuations for crops should be given greater attention in the future.

Ancient relaxation of an obligate short-day requirement in common bean through loss of CONSTANS-like gene function

Common bean (Phaseolus vulgaris L.) is a major global food staple and source of dietary protein that was domesticated independently in Mexico and Andean South America. Its subsequent development as a crop of importance worldwide has been enabled by genetic relaxation of the strict short-day requirement typical of wild forms, but the genetic basis for this change is not well understood. Recently, a loss of photoperiod sensitivity was shown to result from mutations in the phytochrome photoreceptor gene Ppd/PHYA3 that arose independently within the two major domesticated lineages. Here, we define a second major photoperiod sensitivity locus, at which recessive alleles associate with deleterious mutations affecting the CONSTANS-like gene COL2. A wider survey of sequence variation in over 800 diverse lines, including wild, landrace, and domesticated accessions, show that distinct col2 haplotypes are associated with early flowering in Andean and Mesoamerican germplasm. The relative frequencies and distributions of COL2 and PHYA3 haplotypes imply that photoperiod adaptation developed in two phases within each gene pool: an initial reduction in sensitivity through impairment of COL2 function and subsequent complete loss through PHYA3. Gene expression analyses indicate that COL2 functions downstream of PHYA3 to repress expression of FT genes and may function in parallel with PvE1, the bean ortholog of a key legume-specific flowering repressor. Collectively, these results define the molecular basis for a key phenological adaptation, reveal a striking convergence in the naturally replicated evolution of this major crop, and further emphasize the wider evolutionary lability of CONSTANS effects on flowering time control.

Analysis of lipidomics profile of rice and changes during storage by UPLC-Q-extractive orbitrap mass spectrometry

Rice is one of major staple food worldwide; however, lipid profile of rice and changes during storage remain unclear. Herein, an UPLC-Q-Exactive Orbitrap/MS method was applied for comprehensive lipidomics analysis of rice during storage. A total of 21 subclasses of 277 lipids including fatty acid (36 species), (O-acyl)-1-hydroxy fatty acid (6 species), diglyceride (16 species), triglyceride (89 species), lysophosphatidylcholine (4 species), phosphatidylcholine (14 species), phosphatidylethanolamine (28 species), phosphatidylglycerol (6 species), phosphatidylinositol (11species), cardiolipin (4 species), ceramide (8 species), hexosylceramide (20 species), dihexosylceramide (2 species), trihexosylceramide (1 species), sitosterol ester (1species), acyl hexosyl campesterol ester (5 species), acyl hexosyl sitosterol ester (6 species), digalactosyldiacylglycerol (6 species), monogalactosyldiacylglycerol (9 species), monogalactosylmonoacylglycerol (2 species), and sulfoquinovosyldiacylglycerol (3 species), were first identified in rice during storage. In addition, ceramide, fatty acid, (O-acyl)-1-hydroxy fatty acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, lysophosphatidylcholine, and diglyceride were quantified. Furthermore, statistical analysis of all lipids was performed based on MetaboAnalyst software. The results showed that 22 lipids were significantly different between fresh and stored (360 and 540 days storage) rice demonstrating that lipid composition changed during storage. These different lipids involved 11 metabolic pathways, of which linoleic acid metabolism, glycerophospholipid metabolism, and cutin, suberine and wax biosynthesis were the most relevant. Our study provides useful information for lipidomics profile of rice during storage.

Multivariate correlation analysis of bio-accumulation with soil properties and potential health risks of cadmium and lead in rice seeds and cabbage in pollution zones, China.

A total of 475 representative cultivated land and 435 crop samples from 11 provinces of China were collected, and lead and cadmium in 6 polluted areas by wastewater irrigation and metallurgy industry were analyzed. Rice is the major cash crop and staple food of Chinese residents. Cabbage is also a common food in Chinese daily life. Pollution levels and spatial distribution of soil, rice, and cabbage samples were illustrated on the map. In individual or multiple areas, the multivariate correlation of heavy metal's (cadmium and lead) bio-accumulation in two kinds of plants (rice seed and cabbage) and soil properties (pH, cation exchange capacity, and organic matter) was also investigated. Spearman correlation analysis showed that soil pH values and organic matter (OM) had significant effects on the uptake of Cd and Pb in rice seed: the correlation between lg Cd BCF and pH values is -0.148* (p = 0.026), and the correlation between lg Pb BCF and pH values is -0.339** (p = 0.000). The cation exchange capacity (CEC) and pH significantly impact the Cd and Pb uptake in cabbage: the correlation between lg Cd EF and pH values is -0.199* (p = 0.040), and the correlation between lg Pb EF and pH values is -0.203** (p = 0.009). The Cd and Pb bio-concentration factor BCFs of rice and cabbage decreased with the increase of pH, CEC, and OM, except that Pb BCFs increased with the increase of OM in certain areas. The BCF of Cd varied positively from Pb in cabbage, but inversely with Pb in rice significantly at the 0.01 level (two-tailed Spearman correlation analysis). For the first time, the health quotient (HQ) of Cd and Pb in different regions was also calculated and illustrated on the map. In the soil samples of different areas, average HQ values of Cd and Pb in maturity varied from 0.0003-0.0023 to 0.0051-0.0460; average HQ values of immaturity were 0.0011-0.0103 and 0.0222-0.2014. In the rice samples of different areas, average HQ values of Cd and Pb in maturity varied from 0.305-1.360 to 0.027-0.321; average HQ values of immaturity were 0.601-2.678 and 0.053-0.633. Average HQ values orders of magnitude in the cabbage samples of different areas are the same as that of rice samples, and it is 2-4 orders higher than those in soil. Average HQ values of Cd and Pb in maturity varied from 0.152-1.354 to 0.006-0.506; average HQ values of immaturity were 0.510-4.192 and 0.022-0.207. The total HQ values ingested by children were all higher than those in adults. After investigation, it was found that the total HQ value of mature plants was also higher than that of immature plants. The results of this study would be of great help to future soil remediation with similar types.

Mainstreaming Barahnaja cultivation for food and nutritional security in the Himalayan region.

Selective production of input intensive crops in the present scenario have resulted in productivity stagnation or even decline due to excessive usage of chemicals, affecting the farmers economically. Sustainable agriculture is the way to increase agricultural productivity and economic prosperity by protecting all natural resources. It maintains a balance of soil fertility with crop productivity and nutritional quality. The mixed cropping systems followed earlier in different regions according to their tradition, climatic zone, soil and water conditions were climate-smart approaches to sustainable food production based on practical experiences over the years of old generations. The life style changes, imbalance in farming system in last 70 years and demand for more food as well as declining land resources resulted in intensive agriculture. Besides, least returns and less demand of ethnic crops gave more preference to major staple food crops. Barahnaja is a traditional orphan crops based mixed cropping system practiced in Himalayan region due to its sustainability and assured crop harvest during erratic weather conditions. This traditional farming method is an exemplary scientific approach to derive innovations with respect to productivity, quality, plant soil interactions and organic agriculture. The main focus of the review is to substantiate the characteristics of the traditional mixed cropping system by describing the advantages of the system and opportunities for scientific innovation towards new knowledge and sustainability.

Contamination of rice crop with potentially toxic elements and associated human health risks-a review.

Production of rice, a major staple food crop, should be maintained both quantitatively and qualitatively to assure global food security. In recent decades, various natural (biogeochemical weathering of rocks) and anthropogenic (increased application of agrochemicals, solid and liquid waste discharges from domestic and industrial areas, vehicular pollution, etc.) activities have deteriorated soil and water resources by contributing potentially toxic elements (PTEs) to the environment. Shortage of land resources and requirements of the ever-increasing human population has led to increasing global trend of rice cultivation in contaminated soils, causing accumulation of various PTEs such as arsenic (As), mercury (Hg), cobalt (Co), cadmium (Cd), copper (Cu), lead (Pb), and nickel (Ni) in rice crop, especially in the grains. Rice plants uptake and accumulate PTEs leading to their entry into the food chain. Consumption of rice contaminated with PTEs disturbs the human metabolism as PTEs interfere with different physiological/molecular mechanisms causing various health problems such as weak bones; skin problems; respiratory, cardiovascular, endocrine, nervous, reproductive, and hepatic disorders; and cancer. Possible non-carcinogenic and carcinogenic health risks have been determined in some studies by following the guidelines provided by various governmental or non-governmental agencies. Considering these facts, the present study was conducted to give a broader perspective on rice contamination with various potentially toxic elements, their bioconcentration in rice, associated health risks in human beings, and strategies for bioremediation of soil and water resources to eliminate PTEs.

Rice production in Chitwan district of Nepal: An analysis from economic and environmental perspectives

Rice (Oryza sativa L.) is the major staple food in the Nepalese context. Chitwan district of Nepal was purposively selected to analyze the rice production from the socio-economic and environmental perspective. A total of 100 rice growing farmers, 50 organic and 50 inorganic were selected as the sample for the purpose of the study using the simple random method of sampling. Primary data were collected through a pre-tested semi-structure interview schedule and key informant interviews; secondary data were collected reviewing related publications. Descriptive statistics, multiple regression and chi-square test were used for data analysis. The multiple regression revealed that the four explanatory variables included in the model: age of the household head, primary occupation of the household head, number of family members involved in agriculture and subsidy in inputs for rice farming were found to have positive and statistically significant effect on rice yield (P<0.01). Moreover, chi-square test revealed that the farming practices that contributes to climate change mitigation such as: minimum tillage practice (P<0.05), crop diversification (P<0.01), green manuring (P<0.01), agro forestry practice (P<0.05), incorporating crop residues (P<0.1), weed management practice (P<0.01) and pest management practice (P<0.01)were found to be well adopted by the organic rice farmers, in contrast, the farming practices of inorganic rice farmers were statistically and significantly different in this respect. Government should make such policy that could grave the attention of the Nepalese people towards organic agriculture; moreover, encouraging them to make it their primary occupation.

Growth performance of Major Indian cereals: State level analysis

Cereals are the major staple food, covering about 80 percent of the cropped area in India. In this study, the trends and growth in the area, production, and productivity of major cereals in major producing states of India were analyzed by using secondary data for a period of 22 years from 1994-95 to 2015 16. Compound annual growth rate and instability index were worked out which revealed that among total cereals, not only area under fine cereals (Rice and Wheat) has shown tremendous increase but their share has also increased from 68 to 75 percent, whereas on the other hand coarse cereals (Maize, Jowar, and Bajra) area has declined at -1.12 per cent during the study period. Maize had shown a positive growth rate in the area whereas Jowar and Bajra showed a negative growth rate in the study period. The productivity of major cereals in all states increased significantly. Required production to meet the expected demand is possible only through productivity improvement, as the possibility of area expansion under cultivation is almost nil. Hence, there is greater potential to improve the yield of major cereals crops through biotechnology and genetic engineering, and thus improve farmers’ income.

Weed management in finger millet in India- an overview

Eleusine coracana (L.) Gaertn (finger millet) is one of the most nutritious and major staple food in some states of India. Finger millet is cultivated by using broadcast seeding, row (drill) seeding, and transplanting. In this review, the weeds associated with finger millet in different parts of India are listed, information on reported weed management options in finger millet is synthesized and future weed management research needs are enumerated. Weeds smother the finger millet resulting in significant reduction in the yield by 5 to 70%. The critical period for weed competition in finger millet is the first 4–6 weeks from planting/seeding. Physical/mechanical methods such as hand weeding at 20 and 30 days after planting (DAP) or passing wheal hoe twice with one manual weeding were found to be equally effective. In majority of the studies, inter-cropping was found helpful in reducing weed population substantially. Pre-emergence application of bensulfuron-methyl + pretilachlor, butachlor, isoproturon and post-emergence application of 2,4-D, chlorimuron-ethyl either alone or in combination with other methods were found effective in managing weeds in finger millet. Future research needs are: continuous monitoring of weeds and their shifts, understanding weed ecology and biology, developing improved mechanical tools and weed competitive cultivars along with location specific cost-effective and eco-friendly weed management strategies.

Development and Deployment of Rust Resistant Wheat Varieties in India – Insights from Technology Transfer and Policy Response from the National Seed Production Program

Wheat, a major Indian staple food crop is largely prone to rusts infection. Inter alia, wheat producers have been advocated to adopt the recently released innovative technologies (rust resistant high yielding wheat varieties) developed under the All India Coordinated Research Project to counter the significant yield losses. In the milieu, we investigated the technological trend in terms of seed indent followed by policy options for transfer of technology at farmers’ field. Research findings indicated that the wheat producers adopted old technologies (varieties in our case). In the recent past five years, the trend hasn’t witnessed any drastic change despite several rust resistant varieties have been tested and released for various agro-climatic conditions. Recent varieties take about 5 years to reach their peak adoption since release and is attributed to the innovative transfer of technologies. At the producers’ level, technologies have been upscaled though frontline demonstrations, especially on cluster mode. Demonstrations resulted in an average yield gain of 699 kg/ha for an innovative rust resistant technology (HD 2967) in comparison to its check. Despite the increasing demand and promotion of latest technologies, the need and availability of ‘producer choice variety’ is still persisting which has to be addressed. Innovations like ‘community seed banks’, enabling public-private partnership, contract farming (quadpartite model), participatory demonstrations and blockchain enabled ‘seed tracker’ will strengthen the transfer of technology system in Indian wheat production.

Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9.

SummaryFoods high in amylose content and resistant starch (RS) offer great potential to improve human health and lower the risk of serious noninfectious diseases. Common wheat (Triticum aestivum L.) is a major staple food crop globally. However, the RS contents in the grains of modern wheat varieties are low. Here, we report the generation of high‐amylose wheat through targeted mutagenesis of TaSBEIIa in a modern winter wheat cv Zhengmai 7698 (ZM) and a spring wheat cv Bobwhite by CRISPR/Cas9, respectively. We generated a series of transgene‐free mutant lines either with partial or triple‐null TasbeIIa alleles in ZM and Bobwhite, respectively. Analyses of starch composition, structure and properties revealed that the effects of partial or triple‐null alleles were dosage dependent with triple‐null lines demonstrated more profound impacts on starch composition, fine structures of amylopectin and physiochemical and nutritional properties. The flours of triple‐null lines possessed significantly increased amylose, RS, protein and soluble pentosan contents which benefit human health. Baking quality analyses indicated that the high‐amylose flours may be used as additives or for making cookies. Collectively, we successfully modified the starch composition, structure and properties through targeted mutagenesis of TaSBEIIa by CRISPR/Cas9 in both winter and spring wheat varieties and generated transgene‐free high‐amylose wheat. Our finding provides deep insights on the role of TaSBEIIa in determining starch composition, structure, properties and end‐use quality in different genetic backgrounds and improving RS content with multiple breeding and end‐use applications in cereal crop species through genome editing for health benefits.

Overexpression of rice F-box phloem protein gene OsPP12-A13 confers salinity tolerance in Arabidopsis

Salinity is a serious challenge for agriculture production by limiting the arable land. Rice is a major staple food crop but very sensitive to salt stress. In this study, we used Arabidopsis for the functional characterizati... | Find, read and cite all the research you need on Tech Science Press