Folic acid (FA) has a crucial function in pregnant women and anemic patients. Having adequate FA prior to and during the gestation period can protect the brain of the child and spine from significant birth abnormalities. It reduces the risk of stroke, heart disease and some types of cancers, etc. Thus, FA is employed as a reliable biomarker in the early identification of various disorders. In the present study, we synthesized titanium tetra hydroxide [Ti(OH)4] nanoparticles (NPs) by precipitation method with annealing at 100 °C, which is used to modify carbon paste electrode (Ti(OH)4 /MCPE). FA is detected here by voltammetry method using Ti(OH)4/MCPE in the phosphate buffer of pH 6 and compared with bare and TiO2-400, TiO2-600 and TiO2-800 °C NPs MCPE. Based on ac-impedance spectra, the conductivity of Ti(OH)4/MCPE was found to be higher, and consequently this electrode showed excellent sensitivity of 5.5 µA µM−1 cm−2. Lower detection limit (1.3 nM) was observed in the concentration range of 0.4–30 μM by differential pulse voltammetry. The Ti(OH)4/MCPE was also effective for the determination of FA in real samples such as orange, banana, spinach and tablet. The electrochemical sensor investigated here showed excellent stability (92 %) up to 30 days.