Major Conduit Research Articles

Geomorphology and 10Be chronology of the Last Glacial Maximum and deglaciation in northeastern Patagonia, 43°S-71°W

In southern South America, well-dated glacial geomorphological records constrain the last glacial cycle across much of the former Patagonian Ice Sheet, but its northeastern sector remains comparatively understudied and unconstrained. This knowledge gap inhibits our understanding of the timing of maximum glacier extent, the duration of the glacial maximum, the onset of deglaciation, and whether asynchronies exist in the behaviour of the former ice sheet with latitude, or with location (east or west) relative to the ice divide. Robust glacial reconstructions from this region are thus required to comprehend the mechanisms driving Quaternary glaciations at the southern mid-latitudes. We here present 10Be surface exposure ages from five moraine sets along with Bayesian age modelling to reconstruct a detailed chronology of Last Glacial Maximum expansions of the Río Corcovado glacier, a major former ice conduit of northern Patagonia. We find that the outlet glacier reached maximum expansion of the last glacial cycle during the global Last Glacial Maximum at ∼26.5–26 ka, and that at least four subsequent advances/stillstands occurred over a 2–3 ka period, at ∼22.5–22 ka, ∼22–21.5 ka, ∼21–20.5 ka and 20–19.5 ka. The onset of local ice sheet deglaciation likely occurred between 20 and 19 ka. Contrary to several other Patagonian outlet glaciers, including from similar latitudes on the western side of the Andes, we find no evidence for MIS 3/4 advances. Exposure dating of palaeo-shoreline cobbles reconstructing the timing of proglacial lake formation and drainage shifts in the studied region indicate three glaciolacustrine phases characterised by Atlantic-directed drainage. Phase one occurred from 26.4 ± 1.4 ka, phase two between ∼21 and ∼19 ka and phase three between ∼19 ka and ∼16.3 ka. Exposure dating of ice-moulded bedrock in the interior of the cordillera indicates local disintegration of the Patagonian Ice Sheet and the Atlantic-Pacific drainage reversal had occurred by ∼16.3 ka. We find that local Last Glacial Maximum glacier expansions were coeval with Antarctic and southern mid-latitude atmospheric and oceanic cooling signals, but out of phase with local summer insolation intensity. Our results indicate that local Patagonian Ice Sheet deglaciation occurred 1–2 ka earlier than northwestern, central eastern and southeastern Patagonian outlet glaciers, which could indicate high regional Patagonian Ice Sheet sensitivity to warming and drying during the Varas interstade (∼22.5–19.5 ka).

How fuzzy-trace theory predicts development of risky decision making, with novel extensions to culture and reward sensitivity

Comprehensive meta-analyses of risky decision making in children, adolescents, and adults have revealed that age trends in disambiguated laboratory tasks confirmed fuzzy-trace theory’s prediction that preference for risk decreases monotonically from childhood to adulthood. These findings are contrary to predictions of dual systems or neurobiological imbalance models. Assumptions about increasing developmental reliance on mental representations of the gist of risky options are essential to account for this developmental trend. However, dual systems theory appropriately emphasizes how cultural context changes behavioral manifestation of risk preferences across age and neurobiological imbalance models appropriately emphasize developmental changes in reward sensitivity. All of the major theories include the assumption of increasing behavioral inhibition. Here, we integrate these theoretical constructs—representation, cultural context, reward sensitivity, and behavioral inhibition—to provide a novel framework for understanding and improving risky decision making in youth. We also discuss the roles of critical tests, scientific falsification, disambiguating assessments of psychological and neurological processes, and the misuse of such concepts as ecological validity and reverse inference. We illustrate these concepts by extending fuzzy-trace theory to explain why youth are a major conduit of viral infections, including the virus that causes COVID-19. We conclude by encouraging behavioral scientists to embrace new ways of thinking about risky decision making that go beyond traditional stereotypes about adolescents and that go beyond conceptualizing ideal decision making as trading off degrees of risk and reward.

Pannexin 1 channels and ATP release in epilepsy: two sides of the same coin : The contribution of pannexin-1, connexins, and CALHM ATP-release channels to purinergic signaling.

Purinergic signaling mediated by ATP and its metabolites contributes to various brain physiological processes as well as to several pathological conditions, including neurodegenerative and neurological disorders, such as epilepsy. Among the different ATP release pathways, pannexin 1 channels represent one of the major conduits being primarily activated in pathological contexts. Investigations on in vitro and in vivo models of epileptiform activity and seizures in mice and human tissues revealed pannexin 1 involvement in aberrant network activity and epilepsy, and highlighted that pannexin 1 exerts a complex role. Pannexin 1 can indeed either sustain seizures through release of ATP that can directly activate purinergic receptors, or tune down epileptic activity via ATP-derived adenosine that decreases neuronal excitability. Interestingly, in-depth analysis of the literature unveils that this dichotomy is only apparent, as it depends on the model of seizure induction and the type of evoked epileptiform activity, two factors that can differentially activate pannexin 1 channels and trigger distinct intracellular signaling cascades. Here, we review the general properties and ATP permeability of pannexin 1 channels, and discuss their impact on acute epileptiform activity and chronic epilepsy according to the regime of activity and disease state. These data pave the way for the development of new antiepileptic strategies selectively targeting pannexin 1 channels in a context-dependent manner.

The mechanistic pathways of arsenic transport in rice cultivars: Soil to mouth

Rice cultivars are major conduit of arsenic (As) poisoning to human. We quantified transferability of fifteen rice cultivars representing three groups i.e., high yielding variety (HYV), local aromatic rice (LAR) and hybrid for As from soil to cooked rice and its ingestion led health risk, elucidating the processes of its unloading at five check points. Conducting a field experiment with those cultivars, we sampled roots and shoots at tillering, booting and maturity (with grains), separated the grains into husk, bran and polished rice, cooked it through different methods and analyzed for As. Of the tested groups, As restriction from root to grain followed the order: LARs (94%) > HYVs (88.3%) > hybrids (87.2%). The low As sequestration by LARs was attributed to their higher root biomass (10.20 g hill−1) and Fe-plaque formation (2421 mg kg−1), and lower As transfer coefficients (0.17), and higher As retention in husk and bran (84%). On average, based on calculated four major risk indices, LARs showed 4.7–6.8 folds less As toxicity than HYVs and hybrids. These insights are helpful in advocating some remedies for As toxicity of the tested rice cultivars.

Different types of gravity-driven flow deposits and associated bedforms in the Upper Bengal Fan, offshore Myanmar

This study uses 3D reflection seismic data to investigate how sediment gravity flows contribute to the evolution of the lower continental slope of the Myanmar margin that is part of the Bengal Fan, the largest deep-water fan system in the world. Seafloor and subseafloor data show large sediment wave fields that developed on both flanks of an extensive submarine canyon. The sediment waves exhibit asymmetric stoss and lee sides, wave lengths and heights of 850–3000 m and 25–70 m, respectively, and an upslope direction of migration. Seismic data reveals the presence of multiple fields of vertically stacked sediment waves, interbedded with units characterised by a chaotic seismic facies that accumulate mainly in the troughs of the sediment waves and can be tracked laterally to the adjacent canyons. According to their seismic facies, geometry, and internal architecture these chaotic units are interpreted as debrites. Seismic attributes extracted from different horizons indicate that the sediment waves are dominated by fine-grained sediment, while the debrites are probably associated with coarser-grained deposits. The debrites fill the troughs of the sediment waves, as well as the downstream portions of canyon thalweg, thus flattening the paleo-seafloor. The sediment waves are interpreted as cyclic steps formed by low-density turbidity currents flowing across the slope down to the basin floor, where the change in gradient favours the formation of hydraulic jumps and the transition from supercritical to subcritical flow conditions. A conceptual model for the sediment wave evolution was proposed for the study area, in the transitional environment on the lower slope, with low-density gravity flow deposits and high-density debris flow deposits alternatively accumulating on the major gravity flow conduits.

A metabolic function of the hippocampal sharp wave-ripple.

The hippocampus has previously been implicated in both cognitive and endocrine functions1-15. We simultaneously measured electrophysiological activity from the hippocampus and interstitial glucose concentrations in the body of freely behaving rats to identify an activity pattern that may link these disparate functions of the hippocampus. Here we report that clusters of sharp wave-ripples recorded from the hippocampus reliably predicted a decrease in peripheral glucose concentrations within about 10min. This correlation was not dependent on circadian, ultradian or meal-triggered fluctuations, could be mimicked with optogenetically induced ripples in the hippocampus (but not in the parietal cortex) and was attenuated to chance levels by pharmacogenetically suppressing activity of the lateral septum, which is the major conduit between the hippocampus and thehypothalamus. Our findings demonstrate that a function of the sharp wave-ripple is to modulate peripheral glucose homeostasis, and offer a mechanism for the link between sleep disruption and blood glucose dysregulation in type2 diabetes16-18.

Metasomatism and cyclic skarn growth along lithological contacts: Physical and geochemical evidence from a distal Pb[sbnd]Zn skarn

Distal skarns form by the metasomatic reactions of a host rock induced by far-traveled hydrothermal fluids. Physical and structural characteristics and geochemical patterns of distal PbZn skarn bodies were studied at the Petrovitsa deposit in southern Bulgaria. Skarn bodies formed from the interaction of hydrothermal fluids with reactive host lithologies (marble and gneiss). These fluids were transported along sub-vertical feeder structures and lithological contacts. Epidote skarn developed in gneiss protolith, and pyroxene (johannsenite) skarn developed in marble. Detailed geological mapping, complimented by measurements of the internal structure of the skarn body using pyroxene growth versors, quantifies the propagation direction of the skarn body: 1) away from the major local fluid conduit (feeder structure), and 2) away from lithological contacts between aluminosilicate rock and marble. Such growth suggests that fluid flow was generally orthogonal to the skarn front propagation direction in the pyroxene skarn.Textural, mineralogical and geochemical data from skarn samples reveal multiple growth generations of major skarn calc-silicates epidote and pyroxene. The epidote skarn is characterized by limited spatial distribution and fine-grained epidote/clinozoisite growth associated with massive replacement and sulfide mineralization. The pyroxene skarn consists of acicular clinopyroxene crystals which form spheroidal aggregates with discrete growth banding. These bands are the physical representation of the cyclic fluid pulses which resulted in rhythmic skarn growth marked by geochemical banding. In situ geochemical analyses in the epidote skarn reveal early Al-rich epidote overprinted by Fe-rich epidote associated with higher Mn and Sr contents and irregular compositional banding. Clinopyroxene (Jo60–95) shows general increase in Na, Al, Mn, and REE + Y with distance from the feeder structure and lithologic contacts. These elements correlate with the distance traveled by the hydrothermal fluid from the feeder to the site of skarnification, which we define using a proxy based on the Al content of pyroxene crystals. This reflects an increasing degree of fluid “contamination” by interaction with the aluminosilicate host rocks and functions as a proxy for fluid transport distance. The spatial distribution of trace-elements in pyroxene on an outcrop scale is indicative of discrete pulses of hydrothermal fluid resulting in precipitation of skarn calc-silicates along the increasingly tortuous fluid pathway between the feeder structure and the skarn front, resulting in both the macro- and micro-scale chemical and textural variability of the skarn body.

The ‘Ventriloquism’ of Logoi in the Old English Prose Psalms and ‘Boethius’

ABSTRACT In Old English literature, the actual presence of classical lore is relatively limited. The major conduit of Greek and Roman thought was transmitted to Anglo-Saxon England via Boethius' De Consolatione philosophiae. I will read the Old English Boethius (an Old English translation/adaptation of the sixth-century Consolation of Philosophy by Boethius) as a piece of early English theology and address the polyphony of the divine voice in the light of patristic ideas about theosis. The presence of integumentum is enveloping the text in mystery and providing a clue to the interpretation. It is Heavenly Wisdom that teaches her best pupil Boethius dispassion towards both worldly sorrows and worldly felicities. In the allegorical dialogue between Wisdom and Boethius/Mod, the background ‘chorus of voices' suggests the possibility of a ‘demonstrably Alfredian' heteroglossia, whilst the Alfred-persona himself becomes the vehicle of the Logos, inviting the reader to mediate ‘the drops of the night’.

Paleohydrogeology of the Karstic System of Fuentetoba Spring (Soria, Spain): An Interdisciplinary Approach

As a preliminary phase in the conservation and sustainable management of a karst system in Fuentetoba, Soria, Spain, an interdisciplinary study was carried out to determine its hydrogeological evolution. The hydrogeological history of this aquifer system began during the late Miocene, where discharges were driven by paleo-emergences in the moor, and associated conduits were developed under phreatic (or vadose) conditions—for example, the upper syngenetic galleries in the main known karst cave (Majada del Cura cave). Later on, the nearby karstic massifs, a general flattening of the relief, occurred during the Quaternary Period, during which the karstic base level had been in decline. The aquifer flow was then derived and modified towards the Fuentetoba spring from the earliest stages through the galleries of the aforementioned cave. The observations made in this cave indicate the existence of a unique type of hydrogeological organization. The hypogean network is the result of the excavation of the same water flow that has been entrenching and abandoning the vadose regimen toward the free regimen. The dating of the tuffaceous buildings, associated with the emergences, indicates that since almost the Middle Pleistocene, flow lines have converged in the Fuentetoba spring, inducing a high grade of karstification in the saturated zone of the syncline basin. Moreover, a major drainage conduit was developed by dissolution. During the late Upper Pleistocene, an essential component of the groundwater flow had been derived towards the source of the Mazos River spring. Tufa and paleogour datings in caves indicate that the aquifer has undergone different climatic stages during the latest Quaternary and, therefore, different feeding and recharge processes. These tufas and paleogours are interrelated as well, as they are associated with the warm stages during the most recent Quaternary, according to the regional context, when there was less natural recharge. The simulation of the springs’ flow enabled an approximate quantification of the variation in the aquifer’s hydraulic balance during the different climatic stages. For example, during the last glaciation, the natural recharge was impacted by snowmelt and increased by 160%.

Aquatic insect subsidies influence microbial composition and processing of detritus in near‐shore subarctic heathland

Insects are major conduits of resources moving from aquatic to terrestrial systems. While the ecological impacts of insect subsidies are well documented, the underlying mechanisms by which these resources change recipient ecosystems remain poorly understood. Most subsidy inputs enter terrestrial systems as detritus; thus, soil microbes will likely influence the processing of insect subsidies, with implications for plant community composition and net primary productivity (NPP). In a subarctic ecosystem near Lake Mývatn, Iceland where midge (Diptera: Chironomidae) deposition to land is high, we investigated how insect subsidies affected litter processing and microbial communities. We also evaluated how those belowground effects related to changes in inorganic nitrogen, plant composition and NPP. We simulated subsidies by adding midge carcasses to 1‐m2 heathland plots, where we measured effects on decomposition rates and the plant community. We then studied how fertilization treatments (control, KNO3 and midge‐carcass addition) affected graminoid biomass and inorganic nitrogen in greenhouse experiments. Lastly, we conducted a soil‐incubation study with a phospholipid fatty acid analysis (PLFA) to examine how midge addition to heathland soils affected microbial respiration, biomass and composition. We found that midge addition to heathland soils increased litter decomposition and graminoid plant cover by 2.6× and 2×, respectively. Greenhouse experiments revealed similar patterns, with midge carcasses increasing graminoid biomass by at least 2× and NH4+ concentrations by 7×. Our soil‐incubation study found that midge carcasses elevated microbial respiration by 64%, microbial biomass by 43% and shifted microbial functional composition. Our findings indicate that insect subsidies can stimulate soil microbial communities and litter decomposition in subarctic heathlands, leading to increased NPP and changes in plant community composition.

Characteristics and implications of Albian volcanism in a magma-rich rift basin: Seismic volcano stratigraphic and geomorphological evidence from the Upper Suhongtu Member in the Chagan Sag, China-Mongolia border region

Buried volcanic successions imaged by 3D seismic data reveal facies components of Albian volcanism in the Chagan Sag of the Yingen-Ejinaqi Basin. The volcanic rocks of the Upper Suhongtu Member are composed mainly of vesicular and tight massive basalts and andesites. Three types of volcanoes, including monogenetic volcanoes, flood basalts, and shield volcanoes were recognized through detailed seismic facies analysis. A seismic geomorphological study showed that volcanism declined from Unit 1 to Unit 2, with the decline being strongly associated with the transition from the rift climax to the later syn-rift phase. In Unit 1, a center-type eruption arose at the onset, and was subsequently overwhelmed by multistage basaltic lavas supplied by fissure vents of the Maoxi, Tulage, and Barun faults in response to increased thermal fluid activity. Several cone- and crater-shaped monogenetic volcanoes formed by the center-type eruption that developed in Unit 2. In addition, a large shield volcano supplied by fissure vents and a major volcanic conduit developed in the northwestern part. The Albian volcanism of the Yingen-Ejinaqi Basin and the whole volcanic province contributed to a much hotter and more arid paleoclimate, and their records can be recognized from palynological and elemental geochemical evidence in the Chagan Sag and its surrounding area. Volcanism not only produced widely distributed strongly heterogeneous volcanic reservoirs but also accelerated the thermal maturation of source rocks, contributing to near-source hydrocarbon accumulation in Lower Cretaceous magmatic-rich rift basins of the East Asian region.

Characteristics of Misinformation Spreading on Social Media During the COVID-19 Outbreak in China: A Descriptive Analysis.

BackgroundDuring a public health emergency, social media is a major conduit or vector for spreading health misinformation. Understanding the characteristics of health misinformation can be a premise for rebuking and purposefully correcting such misinformation on social media.MethodsUsing samples of China’s misinformation on social media related to the COVID-19 outbreak (N=547), the objective of this article was to illustrate the characteristics of said misinformation on social media in China by descriptive analysis, including the typology, the most-mentioned information, and a developmental timeline.ResultsThe results reveal that misinformation related to preventive and therapeutic methods is the most-mentioned type. Other types of misinformation associated with people’s daily lives are also widespread. Moreover, cultural and social beliefs have an impact on the perception and propaganda of misinformation, and changes in the crisis situation are relevant to the type variance of misinformation.ConclusionFollowing research results, strategies of health communication for managing misinformation on social media are given, such as credible sources and expert sources. Also, traditional beliefs or perceptions play the vital role in health communication. To sum up, combating misinformation on social media is likely not a single effort to correct misinformation or to prevent its spread. Instead, scholars, journalists, educators, and citizens must collaboratively identify and correct any misinformation.

Model Based Analysis of Innovation in Sustainable Supply Chains

Innovation supports the development of Sustainable Supply Chains (SSCs) through Sustainability-Oriented Innovation (SOI) practices. This study aims to validate and further develop, through its practical application, a baseline SOI practices framework, which can be used as a benchmark model. While applying and validating this framework, several new, conceptually distinctive SOI practices were identified that were not included in the proposed baseline model. This led the authors to propose expanding the initial baseline model with a new category of “transversal” SOI practices, so that the expanded model provides a more comprehensive view of the mechanisms that foster innovation at SSCs. These “transversal” SOI practices are highly contingent on the adopting company’s traits and strategy, thus suggesting that they might be a major conduit through which its strategy is reflected in its SSCM. Besides their transversal and contingent nature, another key trait of the identified SOI practices is their integrated, dynamic, time-dependent, feedback-loop-based interrelationship. These findings suggest the potential effectiveness, for processes such as sustainability innovation that involve substantial time lags, to reposition top management focus and performance evaluation “upstream” in the causal chain. This potentially promising approach involves shifting focus from results (particularly short-term results) to its causes (processes, methods), and systematically managing the ensuing feedback loops.

The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air

Air is a major conduit for the dispersal of organisms at the local and the global scale. Most research has focused on the dispersal of plants, vertebrates and human disease agents. However, the air represents a key dispersal medium also for bacteria, fungi and protists. Many of those represent potential pathogens of animals and plants and have until now gone largely unrecorded. Here we studied the turnover in composition of the entire aerobiome, the collective diversity of airborne microorganisms. For that we performed daily analyses of all prokaryotes and eukaryotes (including plants) using multi-marker high-throughput sequencing for a total of three weeks. We linked the resulting communities to local weather conditions, to assess determinants of aerobiome composition and distribution. We observed hundreds of microbial taxa, mostly belonging to spore-forming organisms including fungi, but also protists. Additionally, we detected many potential human- and plant-pathogens. Community composition fluctuated on a daily basis and was linked to concurrent weather conditions, particularly air pressure and temperature. Using network analyses, we identified taxonomically diverse groups of organisms with correlated temporal dynamics. In part, this was due to co-variation with environmental conditions, while we could also detect specific host-parasite interactions. This study provides the first full inventory of the aerobiome and identifies putative drivers of its dynamics in terms of taxon composition. This knowledge can help develop early warning systems against pathogens and improve our understanding of microbial dispersal.

Characterization of subglacial marginal channels using 3-D analysis of high-density ground-penetrating radar data

AbstractStudying subglacial drainage networks is important for understanding the potential relationship between channel dynamics and rapid glacier recession as well as the role of subglacial channels in subglacial sediment evacuation. In order to delineate the planform geometry of snout marginal subglacial channels, densely spaced ground-penetrating radar (GPR) measurements at a frequency of ~70 MHz were carried out over the snout marginal zones of two temperate glaciers in the southwestern Swiss Alps, the Haut Glacier d'Arolla and the Glacier d'Otemma. Three-dimensional (3-D) data processing and amplitude analysis of the GPR reflection along the glacier bed was used to map the channels. At the Haut Glacier d'Arolla, two relatively straight channels of several meters in width were identified. The positions of these channels correspond well with the locations of channel outlets at the glacier terminus, as well as with fractures appearing on the glacier surface one month after the GPR data acquisition. The latter are believed to represent the beginning of ice collapse above the subglacial channels. At the Glacier d'Otemma, a major subglacial conduit was detected with similar dimensions to those identified at the Haut Glacier d'Arolla, but greater sinuosity. The position of this channel was confirmed by drone-based imagery acquired after glacier margin collapse. Our results confirm that high-density 3-D GPR surveys can be used to map subglacial channels near temperate alpine glacier margins.

Seasonal evidences of microplastics in environmental matrices of a tourist dominated urban estuary in Gulf of Mexico, Mexico

The study evaluated the spatial and seasonal variations of microplastic abundances in water, sediments, and commercial fishes of a semi-urban tourist impacted estuary in the Gulf of Mexico, Mexico. The prevalence of microplastics (MPs) elucidated diffuse sources namely long-range transport, domestic, agricultural, fishing, industrial and recreational activities and the local climatic conditions. Seasonally, the mean abundances of MPs in both water and sediments were high during Nortes (strong winds) followed by the dry and rainy seasons. Overall, black and blue colored MPs dominated the region and all the recovered plastics were fibers. The commercial fishes (n = 187) contained 881 MPs in their gastrointestinal tracts, suggesting that the food web of the estuary is highly prone to microplastic contamination. SEM images of extracted plastic fibers presented surface morphologies that are impacted by physical strains. Further, the elemental characterization of fibers using EDX displayed significant peaks of Al, As, Cl, Cr, Cu, Pb, and Zn that were used as additives during the production of plastics. The main types of polymers included low-density polyethylene, polyester, polypropylene, polycarbonate, rayon, polyvinyl chloride, polyacrylonitrile, polyamide, nylon and polyethylene terephthalate. MP abundances demonstrated in this study elucidate that estuaries are a major conduit for land-derived plastics to the ocean and the results will aid in implementing remedial/clean up actions of the estuary for better conservation of the ecosystem.

A historical overview of long-distance signalling in plants.

Be it a small herb or a large tree, intra- and intercellular communication and long-distance signalling between distant organs are crucial for every aspect of plant development. The vascular system, comprising xylem and phloem, acts as a major conduit for the transmission of long-distance signals in plants. In addition to expanding our knowledge of vascular development, numerous reports in the past two decades revealed that selective populations of RNAs, proteins, and phytohormones function as mobile signals. Many of these signals were shown to regulate diverse physiological processes, such as flowering, leaf and root development, nutrient acquisition, crop yield, and biotic/abiotic stress responses. In this review, we summarize the significant discoveries made in the past 25 years, with emphasis on key mobile signalling molecules (mRNAs, proteins including RNA-binding proteins, and small RNAs) that have revolutionized our understanding of how plants integrate various intrinsic and external cues in orchestrating growth and development. Additionally, we provide detailed insights on the emerging molecular mechanisms that might control the selective trafficking and delivery of phloem-mobile RNAs to target tissues. We also highlight the cross-kingdom movement of mobile signals during plant-parasite relationships. Considering the dynamic functions of these signals, their implications in crop improvement are also discussed.

Limited dispersal of riverine litter onto nearby beaches during rainfall events

Rivers are assumed to be one of the major conduits for plastics entering the sea, but little is known about the interchange of plastic litter between rivers and coastal waters. We measured the amount of litter stranding on the beach around the mouth of a seasonal river in Cape Town, South Africa, to infer litter dispersal distances. Prior to the mouth opening, litter was fairly uniformly distributed along the 2.4 km study beach. Once the mouth was opened for the first major winter storm, most litter stranded within 0.5 km of the river mouth. Beach litter loads peaked during rain events and decreased after the first rainfall event, presumably due to flushing of accumulated litter in the coastal wetland. However, litter loads within the wetland remained high, indicating that only a proportion of litter flushes during floods. Experimental deployments of marked litter items confirmed that most floating litter strands close to the mouth, although less buoyant items travelled farther than more buoyant items. Exchanges occurred in both directions, with marine litter found up to 1.2 km inland in the estuary. On a rising tide, more litter items moved into the estuary than into the sea, despite strong offshore winds. Our results illustrate the complex interplay of litter between rivers and the sea, and suggest that a large proportion of floating litter carried down small rivers washes ashore shortly after entering the sea. Cleaning beaches around river mouths, especially after rain events, will help to reduce leakage of plastic from land-based sources into the sea.

Increased SOX2 expression in three-dimensional sphere culture of dental pulp stem cells

Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.

Tidal sedimentary dynamics of the Early Pleistocene Messina Strait (Calabria, southern Italy) based on its modern analogue

The Messina Strait excursion opens a series of geological field trips associated with the 10th International Congress of Tidal Sedimentology (Tidalites), Matera, Italy, 5-7 October 2021. This guide aims at documenting a number of selected outcrops located along the eastern margin of the modern Messina Strait, in order to illustrate the sedimentary dynamics of the Early Pleistocene tide-dominated Messina Strait. Since the Pliocene, this extensional basin separated Sicily from Calabria, forming a wide non-tidal seaway. Successively, this basin turned into a ca. 10-15 km-wide and 40 km-long, tide-dominated strait during the Early Pleistocene, prior to its definitive closure following a Middle Pleistocene phase of tectonic uplift. As for today in its modern analogue, the ancient strait acted as a major conduit for marine water exchanges between the Ionian and the Tyrrhenian seas. Semi-diurnal, reverse bidirectional tidal currents flowed in phase opposition parallel to the strait margins, being subject to tidal amplification due to bathymetric restriction across the shallower strait-centre zone. This oceanographic setting partitioned the strait into specific environments. Nowadays, their sedimentary record is exposed in a series of outcrops across the western (Sicily) and eastern (Calabria) margins of the modern strait.A series of stops along a south-to-north transect covers a total distance of ca. 20 km. Outcrops of the first day show coarse-grained deposits lying adjacent to a block-faulted central horst and transgressively overlain by cross-stratified, mixed bioclastic-siliciclastic arenites. These strata record bypass and residual sedimentation in the strait-centre zone of the ancient strait. The second day, large- and medium-scale cross-stratification exhibiting a variety of tidal sedimentary indicators are observed, interpreted as the ancient northern tidal dune field. The third day focuses on one major section representing the north-eastern flank of the ancient strait, where subaqueous canyon-fill strata, mass-wasting deposits and tide-influenced delta front-facies are exposed.