Major Categories Research Articles

Exploration in Deep Reinforcement Learning: From Single-Agent to Multiagent Domain.

Deep reinforcement learning (DRL) and deep multiagent reinforcement learning (MARL) have achieved significant success across a wide range of domains, including game artificial intelligence (AI), autonomous vehicles, and robotics. However, DRL and deep MARL agents are widely known to be sample inefficient that millions of interactions are usually needed even for relatively simple problem settings, thus preventing the wide application and deployment in real-industry scenarios. One bottleneck challenge behind is the well-known exploration problem, i.e., how efficiently exploring the environment and collecting informative experiences that could benefit policy learning toward the optimal ones. This problem becomes more challenging in complex environments with sparse rewards, noisy distractions, long horizons, and nonstationary co-learners. In this article, we conduct a comprehensive survey on existing exploration methods for both single-agent RL and multiagent RL. We start the survey by identifying several key challenges to efficient exploration. Then, we provide a systematic survey of existing approaches by classifying them into two major categories: uncertainty-oriented exploration and intrinsic motivation-oriented exploration. Beyond the above two main branches, we also include other notable exploration methods with different ideas and techniques. In addition to algorithmic analysis, we provide a comprehensive and unified empirical comparison of different exploration methods for DRL on a set of commonly used benchmarks. According to our algorithmic and empirical investigation, we finally summarize the open problems of exploration in DRL and deep MARL and point out a few future directions.

An update on nonsteroidal anti-inflammatory drug-induced urticaria.

Hypersensitivity reactions to non-steroidal anti-inflammatory drugs (HR-NSAIDs) are common adverse events related to the widespread use of over-the-counter NSAIDs for the treatment of a variety of inflammatory conditions. Urticaria is the most commonly reported immediate cutaneous clinical sign of HR-NSAIDs, but it can be a manifestation of pathophysiologically different clinical entities that require different therapeutic strategies. The aim of this study is to ease the identification of the correct phenotype of HR-NSAIDs in patients reporting urticaria associated with the intake of NSAIDs and provide updated information about their diagnosis and management. The study is a narrative review conducted by collecting the most relevant and up-to-date data related to the classification, pathophysiology, severity, and prognosis of NSAID hypersensitivity reactions. PubMed and Embase scientific databases were used as search engines to select relevant articles. Patients developing HR-NSAIDs can be divided into two categories: selective responders (SR), who develop reactions after the administration of a single specific NSAID due to an underlying IgE or T-cell mediated hypersensitivity mechanism, or cross-intolerant (CI), who develop reactions to more than one chemically unrelated NSAIDs due to abnormalities in the biochemical pathways related with prostaglandin metabolism, independently from an underlying immunological mechanism. Five major different categories of HR-NSAIDs have been identified: NSAIDs-exacerbated cutaneous disease (NECD), NSAIDs-induced urticaria/angioedema with/without respiratory and systemic symptoms of anaphylaxis (NIUAA), and NSAIDs-exacerbated respiratory disease (NERD), which are developed by CI patients, and single NSAIDs-induced urticaria, angioedema and/ or anaphylaxis (SNIUAA) and single NSAIDs-induced delayed hypersensitivity reactions (SNIDHR), which are developed by CI patients. In vivo and in vitro diagnostic tests have rarely been shown to be reliable in all these entities and therefore are not routinely used in clinical practice. The management in SR patients consists of strict avoidance of the culprit drug, while for cross-intolerance reactions oral tolerance tests with safe alternative drugs (e.g. weak COX-1 inhibitors or selective COX-2 inhibitors) can be performed. HR-NSAIDs are being observed with increasing frequency, however, the pathogenesis behind some NSAIDS-associated clinical entities is still unclear. Diagnosis is mostly based on a thorough clinical history and confirmed by a drug challenge test. Clinical management is based on strict avoidance and use of alternative tolerated medications. Overall, all therapeutic decisions depend on the correct identification of the type of reaction the patient experienced.

GRIDS: Personalized Guideline Recommendations while Driving Through a New City

Drive tourism has become increasingly popular in the past decade; however, driving through a new city is challenging because the road and traffic environments vary significantly across cities. A driver used to driving in one city may face severe difficulty in adapting to a different driving environment, leading to road fatalities. This article develops GRIDS , an explainable model for guidelines recommendation for inter-domain driving safety, which learns the driving rules behind the changing environment and recommends the necessary personalized guidelines to a driver while driving through a new city. We develop an explainable domain adaptation model to provide customized recommendations in terms of driving guidelines, broadly categorized into four major feature categories of a driving environment. A thorough evaluation over the CARLA driving simulator shows that the recommendations generated through GRIDS can help improve driving safety.

Verb-centered means of expressing self-presentation in the English-language job-hunting discourse

This article presents a semantic, syntactic, and contextual analysis of the language means of self-presentation, conceptualized as the dominant speech strategy in the job-hunting discourse. The scope of our research is limited to the written variety of texts expressed via the genres of CVs/resumes and cover letters. The material was taken from English-language job-hunting resources: Balance, Career Addict, Career Blog, Indeed, Resume.io, StandOut CV, and Zety. It is stipulated that self-presentation can be viewed as a system of interrelated speech tactics: self-nomination, self-description, presentation of professional achievements, description of professional responsibilities, and identification with an ideal candidate type. Each tactic has its unique semantic profile. All self-presentation tactics roughly fall into two major categories—nominative and verb-centered. The latter category, which incorporates the tactics of presentation of professional achievements and description of professional responsibilities, was the primary focus of this research. A careful semantic and syntactic analysis revealed that active verbs with two-complement (in some cases, three-complement) valency are the core of the aforementioned tactics. This leads us to believe that the actant situation almost always includes the agent and theme, sometimes the benefactive. As a result of semantic analysis, lexical units were classified according to their sentimental modality—positive, negative, or neutral. Our research documented the prevalence of positive evaluation semantics in self-presentation units. A syntactic analysis was employed to identify the formal aspects of sentences containing self-presentation. The analysis showed that most sentence structures fall into three basic types of syntax reduction: a) subject omission ([I] perform regular check-ups); b) omission of the subject and the link verb ([I am] professionally trained); c) the omission of the subject and the verb of possession ([I have] numerous awards). The authors conclude that the self-presentation blueprint of verb-centered constructions identified in this article persists across all verb-centered tactics proving to be an effective means of constructing written job-hunting discourse.

Nonlinear effect of Islamic financing on economic stability: A Case of equity and debt financing

The main purpose of this study is to differentiate between the effects of two major categories of financing of Islamic banks on the economic stability of the selected countries. This study determines the nonlinear effect of equity-based and debt-based financing of Islamic banks to assess how the stability of the economy can be achieved. This study acquires the data on Islamic banking product-wise financing at a national level from the Islamic Financial Service Board (IFSB), and the data on industrial production index is selected from International Financial Statistics for selected countries. The sample size comprises of all the countries included in the IFSB. The data ranges from 2014Q1 to 2019Q4. The economic stability is estimated using the GARCH volatility approach, and the nonlinear ARDL model is used to determine the causal effect of Islamic banking product wise financing. This study clarifies the empirical relationship between Islamic banking equity-based financing and debt-based financing and whether macroeconomic stability is linear or quadratic in terms of the marginal effect of financing, which helps policymakers to constitute strategies for expanding the size of Islamic banking financing. The results identified the inverted U-shaped effect of equity-based financing types on output instability. At the same time, both financing types are not causing price instability. The outcomes are instruments for the central bank to optimize the Islamic banking financing structure to achieve the goal of product-wise financing of economic stability.

Social life results in social stress protection: a novel concept to explain individual life-history patterns in social insects.

Resistance to and avoidance of stress slow aging and confer increased longevity in numerous organisms. Honey bees and other superorganismal social insects have two main advantages over solitary species to avoid or resist stress: individuals can directly help each other by resource or information transfer, and they can cooperatively control their environment. These benefits have been recognised in the context of pathogen and parasite stress as the concept of social immunity, which has been extensively studied. However, we argue that social immunity is only a special case of a general concept that we define here as social stress protection to include group-level defences against all biotic and abiotic stressors. We reason that social stress protection may have allowed the evolution of reduced individual-level defences and individual life-history optimization, including the exceptional aging plasticity of many social insects. We describe major categories of stress and how a colonial lifestyle may protect social insects, particularly against temporary peaks of extreme stress. We use the honey bee (Apis mellifera L.) to illustrate how patterns of life expectancy may be explained by social stress protection and how modern beekeeping practices can disrupt social stress protection. We conclude that the broad concept of social stress protection requires rigorous empirical testing because it may have implications for our general understanding of social evolution and specifically for improving honey bee health.

Sustainability Tools in Cultural Heritage: Life Cycle Assessment Examining Crates for Heritage Objects

ABSTRACT The Sustainability Tools in Cultural Heritage (STiCH) https://stich.culturalheritage.org project informs cultural heritage professionals about sustainable choices through life cycle assessment (LCA). This paper focuses on one study https://stich.culturalheritage.org/crates/ that examines the environmental impacts of transporting cultural heritage. The study models four crate types crates single trip, round trip, and reusable. For each crate, three major material categories (exterior, interior, and wrapping) were analyzed, giving each configuration an environmental profile. The study compares these crate options over their life cycles, including impacts from material production, transportation, and disposal. The results show that reusable crate have lower impacts than wooden crates across a broad range of environmental considerations. Transportation dominates reusable crates and is relatively less important for single-trip crates. For single-trip and round-trip crates, foams can be a major source of greenhouse gas emissions. Actions that would reduce impacts include reusing the crate itself or its disassembled components. Lowering the weight of the crate, possibly by using lighter materials for the crate shell, would reduce impacts from transport. These findings can encourage further research and exploration of sustainable materials that perform similarly to the items they replace.

Maternity Service Professionals’ Perspectives of “the Good, the Bad, and the Possible” in Australian Maternity Services: A Content Analysis

OBJECTIVE:In 2019, the Australian government released a strategy for maternity care:Woman-centred care strategic directions for Australian maternity services(WCC Strategy), with mixed responses from providers and consumers. A public lecture and expert panel held in March 2021, titled “The Good, the Bad, and the Possible,” facilitated critique and feedback on the WCC Strategy and the Australian maternity system. The aim of this article was to report the findings of a qualitative analysis of participants’ views regarding the current perceptions of Australian maternity care.METHODS:A content analysis of responses to questions posed during the lecture and the commentary provided by participants was undertaken, utilizing a predetermined framework of “the Good, the Bad, and the Possible.”RESULTS:Approximately 700 participants, mainly from Australia, attended the event. The majority were midwives. Under the guiding framework, the category “When it works well” captured “the Good” aspects of maternity care. Under “the Bad,” major categories included “the system is broken,” “roadblock to maternity service reform,” and “not being seen or heard.” Suggestions for improvement, and hope that these will be enacted, were encompassed in “the Possible” categories: “what is needed” and “we’re in this together.”CONCLUSION:Participants identified widespread maternity system issues resulting in women’s needs not being met. Midwives indicated feeling invisible, as their repeated attempts at improvement were thwarted. Hierarchical power imbalances and medical privilege were suggested to be obstructions to respectful partnerships and expansion of midwifery models of care, preventing women from accessing safe birth options of their choice.

Environmental impact and damage assessment of oil trunkline transportation by life cycle assessment method

AbstractAs climate issues gradually attract public attention worldwide, the operation of crude oil pipelines has been attached with new emission‐reduction targets. Though previous studies concerning life cycle assessment (LCA) of oil and gas pipelines have estimated the carbon footprint to some extent, there is a lack of research on life cycle carbon emissions that take the process of crude oil pipelines transportation. the main objective of this study is to build a comprehensive LCA model of carbon footprint for crude oil pipeline systems. A gate‐to‐gate analysis is provided for the LCA model, which means that the entire pipeline's life cycle from the extraction to disposal is considered. The LCA approach used both midpoint and endpoint impact assessment methods to evaluate the environmental impacts of crude oil pipeline transportation in Iran. The study analyzed the major environmental impact categories, such as greenhouse gas emissions, energy consumption, water usage, and solid waste generation. The results showed that the crude oil pipelines transportation has significant environmental impacts in Iran. The three highest impacts on the midpoint scale are marine ecotoxicity by 61.54 kg 1,4‐DCB, terrestrial ecotoxicity by 52.26 kg 1,4‐DCB and fossil resource scarcity by 40.22 kg oil eq. The endpoint impacts of three stations were also compared. The Chamran, Abadan and Zanganeh stations have 95.44%, 94.22%, and 6% impacts on human health, respectively. Also, Abadan station has the largest amount of damage to ecosystems and resources with 96.65% and 80.02%, respectively. The findings of this study could be used by policymakers, oil companies, and other stakeholders to make informed decisions and implement strategies to reduce the environmental impact of oil transportation in Iran.

Impact of Climate Change Assessments on Horticultural Crop Dynamics in Tamil Nadu, India

Aim: This study aims to analyze the dynamics of horticultural crop production in Tamil Nadu amidst climate change, focusing on trends, challenges, and opportunities across diverse agro-climatic zones, primarily considering variations in rainfall.
 Data: The research utilizes comprehensive datasets spanning a decade (2012-13 to 2021-22) encompassing records of rainfall, horticulture cultivation area, and production. The study focuses on major horticultural crop categories, including fruits, vegetables, plantation crops, spices and condiments, medicinal and aromatic crops, and flowers.
 Objective: (1) analyze compound annual growth rates of area, production, and productivity of key horticultural crops in Tamil Nadu, (2) conduct decomposition analysis over a decade for horticultural crops, and (3) assess climate change impact on agricultural production in Tamil Nadu focusing on rainfall patterns.
 Methodology: The study utilizes various analytical tools, including Compound Annual Growth Rate (CAGR) analysis, percentage analysis, decomposition analysis, and the Cobb-Douglas production function. Secondary data from government publications, including the Season and Crop Report of Tamil Nadu and the Department of Horticultural and Plantation Crop Report of Tamil Nadu, are utilized for analysis.
 Results: The analysis reveals intricate patterns influenced by climate variability, with certain zones demonstrating harmonious growth, while others contend with challenges related to cultivation and productivity. The decomposition analysis highlights the respective contributions of changes in cultivation area and crop yield to variations in production, underscoring the impact of climate change on agricultural dynamics. Additionally, the Cobb-Douglas production function analysis indicates significant effects of both rainfall and cultivation area on horticultural production, emphasizing the multifaceted nature of factors influencing production trends.

A systematic review on deep learning-based automated cancer diagnosis models.

Deep learning is gaining importance due to its wide range of applications. Many researchers have utilized deep learning (DL) models for the automated diagnosis of cancer patients. This paper provides a systematic review of DL models for automated diagnosis of cancer patients. Initially, various DL models for cancer diagnosis are presented. Five major categories of cancers such as breast, lung, liver, brain and cervical cancer are considered. As these categories of cancers have a very high percentage of occurrences with high mortality rate. The comparative analysis of different types of DL models is drawn for the diagnosis of cancer at early stages by considering the latest research articles from 2016 to 2022. After comprehensive comparative analysis, it is found that most of the researchers achieved appreciable accuracy with implementation of the convolutional neural network model. These utilized the pretrained models for automated diagnosis of cancer patients. Various shortcomings with the existing DL-based automated cancer diagnosis models are also been presented. Finally, future directions are discussed to facilitate further research for automated diagnosis of cancer patients.

Rapid risk assessment of plant pathogenic bacteria and protists likely to threaten agriculture, biodiversity and forestry in Zambia

A prioritisation study was conducted to address the lack of adequate information about potential pests likely to be introduced in Zambia and become invasive. The study was conducted by subject matter experts from relevant institutions in and outside Zambia. Although this study focused on major pest categories, this paper only addresses bacteria and Protista. A list of 306 bacterial and 10 Protista species adjudged to affect plants was generated using CABI’s Horizon Scanning Tool. The 316 (total) pest species were refined to focus on pests that affect value chains important to Zambia’s economy. This resulted in a final list of 133 bacteria and eight Protista. Four additional bacteria species considered of phytosanitary interest were added and all 137 bacteria and eight Protista species were subjected to a rapid risk assessment using agreed guidelines. Vectors reported to transmit any of the pathogenic organisms were also subjected to a risk assessment. A proportion of 53% (n = 77 of 145) comprising 73 bacteria and four Protista species were reported as present in Africa. Of these, 42 (57%, n = 73) bacterial species and two (n=4) Protista species were reported in neighbouring countries. Considering a cut-off of 54, the highest scoring pests were 40 bacteria (highest score of 140) and three Protista (highest score of 125). Three actions were suggested for high-scoring pests, a detection surveillance, a pest-initiated pest risk analysis (PRA) or a detection surveillance followed by pest-initiated PRA. A “no action” was suggested where the risk was very low although, for some pathogenic organisms, a “no action” was followed by periodic monitoring. This information will contribute towards proactive prevention and management of biological invasions.

Vegetation Type Mapping in Southern Patagonia and Its Relationship with Ecosystem Services, Soil Carbon Stock, and Biodiversity

Vegetation Type (VT) mapping using Optical Earth observation data is essential for the management and conservation of natural resources, as well as for the evaluation of the supply of provisioning ecosystem services (ESs), the maintenance of ecosystem functions, and the conservation of biodiversity in anthropized environments. The main objective of the present work was to determine the spatial patterns of VTs related to climatic, topographic, and spectral variables across Santa Cruz province (Southern Patagonia, Argentina) in order to improve our understanding of land use cover at the regional scale. Also, we examined the spatial relationship between VTs and potential biodiversity (PB), ESs, and soil organic content (SOC) across our study region. We sampled 59,285 sites sorted into 19 major categories of land cover with a reliable discrimination level from field measurements. We selected 31 potential predictive environmental dataset covariates, which represent key factors for the spatial distribution of land cover such as climate (four), topography (three), and spectral (24) factors. All covariate maps were generated or uploaded to the Google Earth Engine cloud-based computing platform for subsequent modeling. A total of 270,292 sampling points were used for validation of the obtained classification map. The main land cover area estimates extracted from the map at the regional level identified about 142,085 km2 of grasslands (representing 58.1% of the total area), 38,355 km2 of Mata Negra Matorral thicket (15.7%), and about 25,189 km2 of bare soil (10.3%). From validation, the Overall Accuracy and the Kappa coefficient values for the classification map were 90.40% and 0.87, respectively. Pure and mixed forests presented the maximum SOC (11.3–11.8 kg m−2), followed by peatlands (10.6 kg m−2) and deciduous Nothofagus forests (10.5 kg m−2). The potential biodiversity was higher in some shrublands (64.1% in Mata Verde shrublands and 63.7% in mixed shrublands) and was comparable to those values found for open deciduous forests (Nothofagus antarctica forest with 60.4%). The provision of ESs presented maximum values at pure evergreen forests (56.7%) and minimum values at some shrubland types (Mata Negra Matorral thicket and mixed shrubland) and steppe grasslands (29.7–30.9%). This study has provided an accurate land cover and VT map that provides crucial information for ecological studies, biodiversity conservation, vegetation management and restoration, and regional strategic decision-making.

Late Modern Projectivity and Existentialism

The article addresses the issue of the relationship between existentialism and late modern projectivity interpreted in terms of project work and of biographical projectivity. It does so from the perspective of transformations of subjectivity in modernity and late modernity, and, therefore, from the perspective of the process of individualization. The objective is to answer the question of the belonging of existentialism to one of these sociocultural formations. For these purposes, some major categories of existentialism such as project, anxiety, and temporality are analyzed. Their analysis leads to the conclusion that there is a close, structural correlation between existentialism and sociocultural structures of modernity as well as that existentialism, in contrast to late modern projectivity, has some emancipatory potential related to its alienating status in modernity. The article refers to the methodology of qualitative sociology.

The Mental Well-Being and Inclusion of Refugee Children: Considerations for Culturally Responsive Trauma-Informed Therapy for School Psychologists

Refugee children displaced by war suffer incredible amounts of physical and psychological trauma during and post-displacement. War is not partial to children, and they are subjected to as much extreme violence as adults. This paper explores the mental health of refugee children following war and was guided by the research question: What are the mental health experiences of refugee children displaced by war situations? A secondary data analysis was conducted on publicly available documentary short films and instructional videos on psychological therapy with refugee children and families. The data were analysed through Qualitative Conventional Content Analysis (QCCA). Three major categories emerged regarding the well-being of refugee children: (1) children are not spared from war trauma, (2) children live in perpetual fear and anxiety, and (3) war-related violence ignites aggressive behaviours in children. To increase positive mental health outcomes, school psychologists need to implement trauma-informed therapy that focuses on decreasing psychosocial reactions to war. Culturally responsive therapy is recommended as it places indigenous ways of being at the centre of the healing process.

Developing a tool to optimize research on antioxidants for rooster semen cryopreservation

This study aimed to develop a tool for predicting the potential impact of research studies involving the effect of antioxidants in rooster semen freezing diluent, depending on the variables that have been studied. To achieve this, a comprehensive meta-analysis of fifty-eight research documents was performed. Sixty-two traits were sorted into four major categories: study demographics, study design-related parameters, rooster semen quality-related parameters, and fertility level indicators. The quartile determination of each research document was collected from the Journal Citation Reports database. After twenty-five testing rounds, all variables that showed multicollinearity problems were discarded from further analyses (VIF < 5). HOST, pellet volume, mass motility, light hours, and sperm concentration were the most influential traits for the classification of papers in different quartiles (Wilks’ lambda: 0.797, 0.891, 0.895, 0.896, and 0.904, respectively). The research was validated as reported in the cross-validation analysis, with 93.60% of papers being correctly classified within their group. The present research assists researchers not only in the decision-making process for journals in which to publish the outcomes of their studies, but also to seek for the inclusion of parameters which attract a wider interest for the matter from scientific readers. This leads to the optimisation of resources in studies evaluating the effect of antioxidants in poultry reproduction by identifying the most scientifically relevant variables and those which in trun will lead toa greater impact on research publications.

Self-management of diabetes in adolescence: experience of Portuguese young adults and their parents

Objective To identify the factors that facilitate or hinder the construction of autonomy in adolescence through the experience of young adults with type-1 diabetes and their parents. Methods This was a qualitative, descriptive, and exploratory study. Two focus group interviews were conducted: one with nine young adults who were experts in managing their illness and the other with seven parents. Thematic and categorical content analysis was used for data analysis, with particularities of a focus group interview and the use of the NVIVO 12 software. Results Two major categories and ten subcategories related to factors that facilitated (support systems, knowledge, diet, insulin pump, early responsibility for managing therapy, and characteristics of young people) and hindered (therapeutic regimen, stigma, attitude of health professionals, characteristics of young people, and knowledge) the development of autonomy in disease management emerged. Conclusion Autonomy in the management of diabetes involves several challenges for adolescents, which requires adaptation of attitudes and interventions by professionals. In addition to the traditional management of the health condition, addressing issues related to the socialization of adolescents is essential, looking for innovative strategies that promote coping and quality of life. The results of this study make it possible to reflect on the therapeutic relationship with adolescents, emphasizing the importance of individualizing care and innovative responses to their specific needs.

Maximising CO2 Sequestration in the City: The Role of Green Walls in Sustainable Urban Development

Environmental issues are a pressing concern for modern societies, and the increasing levels of atmospheric CO2 have led to global warming. To mitigate climate change, reducing carbon emissions is crucial, and carbon sequestration plays a critical role in this effort. Technologies for utilising CO2 can be divided into two major categories: direct use and conversion into chemicals and energy, and indirect use as a carbon source for plants. While plants’ ability to absorb and store CO2 makes them the best CO2 sink, finding suitable urban areas for significant green spaces is a challenge. Green walls are a promising solution, as they require less land, provide more ecosystem services than horizontal systems do, and can contribute to reducing environmental problems. This study evaluates the conceptual potentials and limitations of urban biomass circulation in terms of energy production, food production, and CO2 consumption, focusing on growth-promoting bacteria, urban agriculture, and vertical systems. The aim of this research is discovering new methods of carbon sequestration using multi-purpose green walls to achieve sustainable urban development and CO2 reduction strategies to contribute to a more sustainable future.

The relationship between psychopathy facets and types of criminal offences

AbstractThe Psychopathy Checklist—Revised (PCL‐R) is a widely used measurement of psychopathy comprising interpersonal, affective, lifestyle, and antisocial facets representing narrow‐band characteristics associated with the construct. However, there is little research on whether the distinct facets of psychopathy show specialised relationships with different types of criminal offences. The PCL‐R was administered to 138 adults incarcerated at a county jail. Arrests were coded into broad categories (i.e., crimes against persons, property or society), as well as for specific offence types (e.g. burglary, assault) using US crime definitions. Zero‐order correlations and zero‐inflated regression models showed significant relationships between: the lifestyle facet and property and societal crimes, mainly drug offences; the affective facet and crimes against persons; and the antisocial facet with all three major crime categories, as well as burglary, motor vehicle theft, robbery and assault. Overall, these results indicate that the PCL‐R facets offer unique information about specific forms of criminal behaviour.

Analysis of pyranoanthocyanins, polymeric pigments and colour parameters in Port wines

Context &amp; aim: The two major categories of Port wines, Tawny and Ruby, are defined by their ageing conditions, with associated oxidative conditions being much more pronounced in the case of the former than the latter. The main aim of this study was to determine if, independently of the producer, all Port wines can be grouped into these styles based on their chromatic and pigment characteristics. Methods: A total of 32 Port wine samples comprising eight different styles were provided by five different producers of the Douro region in Portugal for this work. All the samples were filtered prior to analysis for anthocyanin determination using an HPLC-DAD-ESI/MS, and unfiltered samples were used for the determination of colour parameters and CIE Lab coordinates. The samples were measured in triplicate for statistical analysis. Results: The chromatic analysis of the Port wines showed Ruby style Ports to have a higher colour intensity and lower hue (°) than Tawny style Ports. The PCA analysis of colour parameters and CIE Lab coordinates clearly shows two separate clusters representing Ruby styles and Tawny styles respectively. Overall, Tawny style Ports had traces of or non-detected anthocyanins monoglucosides, a few styles having &lt; 10 mg/L in the form of malvidin-3-O-glucoside equivalent; meanwhile Ruby styles showed higher concentrations of anthocyanins as detected by HPLC, sometimes reaching values close to 100 mg/L. The major anthocyanin family found across all wines (even in trace amounts) were pyranoanthocyanins, specifically vitisin A and coumaroylated vitisin A. Main conclusions: The results prove that in terms of all the parameters studied there is a clear division between the two major styles of Port wines, which can be attributed to the differences between the ageing process of these two types of wines.