Accurate identification of microstructural degradation that occurs prior to crack coalescence and growth is crucial for continuous online monitoring of creep damage in main stream pipelines. The magnetic non-destructive testing technique is well-established for quantitative property measurement of creep degradation in steels. During the interrupted creep of 12Cr1MoVG steel at 580 ℃ and 110 MPa, the magnetic properties of crept specimens were evaluated using a combination of magnetic hysteresis loop and Barkhausen noise techniques, the microstructural evolution was also examined by means of scanning electron microscopy combined with EBSD method. The microscopic coercive force and Barkhausen noise characteristic values were chosen as the main parameters of diagnosis, as the most sensitive to the microstructural evolution such as dislocations, precipitates and voids, reflects the changes in material condition indicative of early degradation. Besides, these magnetic signatures even can identify the locations that potentially susceptible to future creep damage in the material, which were validated by microhardness measurement, grain misorientation and dislocation density distributions. It suggested that this work could be used as the technical basis for proactive management of material early degradation in the steam pipelines.