Mainland Australia Research Articles

Revision of Xiphydriinae (Hymenoptera: Xiphydriidae) of Australia

AbstractWe re‐diagnoseRhysacephalaBenson, 1954, a genus of xiphydriid woodwasps confined to eastern mainland Australia, Tasmania and Lord Howe Island.Rhysacephala leai(Forsius, 1927), including the first description of the female,Rhysacephala obtusiventris(Rohwer, 1918), andRhysacephala wilsoniBenson, 1954 are redescribed. Six new species from eastern Australia are described:Rhysacephala amplipretarsusJennings, Parslow & Macdonald,sp. nov.,Rhysacephala fulvaJennings, Parslow & Macdonald,sp. nov.,Rhysacephala impensaJennings, Parslow & Macdonald,sp. nov.,Rhysacephala monteithiJennings, Parslow & Macdonald,sp. nov.,Rhysacephala phalarosJennings, Parslow & Macdonald,sp. nov.andRhysacephala tenebrilataJennings, Parslow & Macdonald,sp. nov.A monotypic genus,AustroxiphydaJennings, Macdonald, Schiff & Parslow,gen. nov., is established and includesAustroxiphyda lasalleiJennings, Macdonald, Schiff & Parslow,sp. nov.Keys to identify the 12 Australian species of Xiphydriinae are provided.

An eco-evolutionary rationale to distinguish alien and native status: why the dingo is a native species on mainland Australia

Distinguishing between whether a species is alien or native can be problematic, especially for introduced species that are long-established in new areas outside of their natural range. Transport by humans is the criterion for alien status used by many definitions, whereas arbitrary time since arrival to a location is often used to define native status. Here I propose an eco-evolutionary approach to distinguish between alien and native status and use this to resolve uncertainty in the status of the dingo in Australia. Dingoes were transported to mainland Australia by humans, but more than 4000 years ago, and dingoes now interbreed with feral domestic dogs. Legally, this mix of events has the dingo classified as native in some jurisdictions and alien in others. I suggest that native status for introduced species should be based on (1) whether the species has evolved in their new environment; (2) whether local species recognise and respond to them as they do towards deep endemic native species, and; (3) whether their impacts benchmark against those of a native species or are exaggerated like those of other alien species. Dingoes are behaviourally, reproductively and morphologically different to close ancestors from south-east Asia, and this difference has a genetic basis indicative of evolution in Australia. There is abundant evidence that native prey species on mainland Australia recognise and respond to them as a dangerous predator, which they are. But there is strong evidence that dingo impacts on prey are not exaggerated, with effect sizes from mensurative experiments similar to those of experiments on native predators rather than alien predators. These three lines of evidence suggest dingoes should be considered native to mainland Australia. I suggest this eco-evolutionary approach to defining native status can be helpful in resolving the often-heated debates about when an alien species becomes native.

Contributions to the phylogeny of Lepraria (Stereocaulaceae) species from the Southern Hemisphere, including three new species

We utilized ITS and mtSSU sequence data to study the phylogenetic relationships of Lepraria samples collected in the Southern Hemisphere, including Australia, Chile, New Zealand and Antarctica. Morphological characters and the secondary chemistry of the specimens were also examined. Using a combination of morphological, chemical and molecular data, we identified nine lineages in our material. The lineages are: Lepraria toilenae, L. eburnea, L. nothofagi, L. straminea, L. caerulescens, L. finkii, and three previously undescribed clades that are here described as L. chileana, L. neozelandica and L. ulrikii. The first is described from Chile. The second occurs in New Zealand, and the third has an Australasian distribution, occurring in New Zealand, mainland Australia, and Tasmania. In addition, L. straminea is identified as an usnic acid-producing species of the genus Lepraria. Moreover, L. caerulescens is confirmed as a distinct species.

The Game of Guwarra: A game theory-based decision-making framework for site selection of offshore wind farms in Australia

Global concerns around climate change and the volatility of conventional fuel prices have prompted researchers and technologists to make significant efforts to identify and exploit alternative energy sources that are cleaner and more sustainable. Wind energy has seen considerable development among these alternative energy sources, mainly due to its abundance and global availability for extraction and the existing knowledge within the aviation and aerospace fields. Many nations, including European countries, already operate offshore wind farms (OWFs) and are progressively carrying out new projects and expanding on other projects. The Australian offshore environment provides unique opportunities for wind energy extraction, particularly along the southern coast of mainland Australia and the regions around Tasmania, where substantially strong winds blow most of the year. A significant challenge to establishing wind farms is the selection of site locations with optimal outputs. This can become a complex decision-making problem if there are numerous options and no information from previous projects. This paper aims to develop a decision-making framework to select the optimal location for installing OWFs while addressing financial, performance-related, and availability-related objectives. This paper adopts a game-theoretical approach to develop a decision-support tool to account for the interdependencies of influencing factors and possible conflicts amongst the parties. The game model is applied to an OWF development case study in the Bass Strait, known for its dominant and strong winds.

Environmental change during the last glacial on an ancient land bridge of southeast Australia

AbstractAimThe Last Glacial Maximum (LGM) remains an enigmatic period in southeast Australia due to the limited spatial and temporal resolution of its palaeoclimatic records. A major feature of the LGM landscape was the existence of the Bassian Land Bridge, joining Tasmania with the mainland of Australia during periods of low sea level, and potentially facilitating increased biotic movement between these regions. To better understand biogeographical changes on the land bridge and in southeast Australia generally during the LGM, we present a 35 ka‐year palaeoecological record from one of the larger islands of Bass Strait.LocationBass Strait, southeast Australia.TaxonEucalyptus, Poaceae, Monotoca, Myriophyllum, Zygnemataceae, Botryococcus, Pediastrum.MethodsPollen, charcoal, and non‐pollen palynomorphs were analysed in a 1.75‐m sediment core from truwana/Cape Barren Island, Bass Strait, to reconstruct changes in vegetation, fire regimes and lake levels on the Bassian Land Bridge. Results were then compared to existing palaeoenvironmental studies in the region to develop a broader context of the prevailing land bridge environment and the potential influence on biotic dispersals in Australia during the last glacial period.ResultsResults suggest a major vegetation shift on the Bassian Land Bridge in response to the establishment of the LGM climate, with grassland expansion at the expense of woodland from ~29–11 ka. Floristic richness, biomass burned and lake levels also markedly decreased through this period, with the driest interval being ~25–18 ka. Dryland herbaceous taxa dispersed between the mainland and Tasmania when the landbridge was fully exposed.Main conclusionsIn agreement with previous studies, our results show that the dry grassland that dominated southeast Australia (and Southern Hemisphere mid‐latitude areas) during the LGM also extended across the Bassian Land Bridge. The prevailing environment on the land bridge is likely to have exerted a selective influence on biotic dispersals in Australia during glacial periods.

A Cross-Cultural Study of Theory of Mind Using Strange Stories in School-Aged Children from Australia and Mainland China

ABSTRACT To date, cross-cultural studies on Theory of Mind (ToM) have predominantly focused on preschoolers. This study focuses on middle childhood, comparing two samples of mainland Chinese (n = 126) and Australian (n = 83) children aged between 5.5 and 12 years. Strange Stories, the most commonly used measure of ToM, was employed. The study aimed to examine the one- versus two-factor structure and measurement invariance of Strange Stories across two cultures; use the verified invariant model of Strange Stories to compare children’s cognitive and affective ToM across two cultures; and finally, to investigate correlates of individual differences on Strange Stories cross-culturally. Multiple-groups confirmatory factor analysis revealed the measurement invariance of a two-factor model of Strange Stories (cognitive and affective) in both groups. Chinese children performed comparably to Australian children on cognitive ToM stories, but more poorly than Australian children on affective ToM stories. There were cultural differences in the correlates of ToM. The number of older siblings was a positive predictor of cognitive ToM for Chinese children, but a negative predictor of cognitive ToM for Australian children. The findings confirm that Strange Stories is a reliable measure for evaluating ToM in school-aged children from mainland China and Australia and highlight the importance of considering both cognitive and affective aspects of ToM in cross-cultural comparison.

A significant range extension for the Western Soil-Crevice Skink Proablepharus reginae (Glauert 1960) and an updated reptile species list of Cape Range, Western Australia

ABSTRACT We describe a 226 km range extension for the known distribution of the Western Soil-Crevice Skink Proablepharus reginae in Western Australia. This record from Cape Range National Park is the first for this species on the North West Cape and within the Cape Range IBRA sub-region and marks the most westerly record of P. reginae for mainland Australia. This finding raises the terrestrial reptile species richness for the Cape Range peninsula to 90. Previous surveys in this area failed to detect P. reginae, which demonstrates the value of repeated surveys in documenting species richness in remote locations. Furthermore, we provide morphological and ecological data and discuss this record in the contexts of geographic variation and the high number of isolated reptile populations and endemism seen on the Cape Range peninsula.

Species distribution models for conservation: Identifying translocation sites for eastern quolls under climate change

Conservation and natural resource management are frequently hampered by poor understanding of how species distributions have changed over time. Species Distribution Models (SDMs) correlate known occurrences with environmental variables to predict a species’ potential range. These models can then be projected to unsurveyed areas or time periods to overcome gaps in data on species’ distribution. The eastern quoll Dasyurus viverrinus is restricted to Tasmania but until recently occurred over a large area of south-eastern mainland Australia. We used SDMs to reconstruct the distribution of climates suitable for D. viverrinus during the mid-Holocene and present day and identified areas likely to remain suitable in the future which could be prioritised for conservation. Climatically suitable habitat has mostly remained stable since the mid-Holocene, with some expansion. The historic contraction of the species’ distribution was evidently due to threatening processes other than climate change, which are still operating. Under current conditions, large areas of south-eastern Australia still provide suitable habitat for eastern quolls and will continue to do so under predicted climate change scenarios for 2055. Suitable areas include offshore islands, in particular islands within the Nooramunga Marine and Coastal Park in Victoria, and islands that make up the Furneaux Group in the Bass Strait. Translocations to these islands would establish populations in climatically suitable areas separate from threatening processes.

Sniffing out danger: rapid antipredator training of an endangered marsupial

Globally, predator aversion training has assisted naive prey species to learn to evade introduced predators, improving translocation success. Eastern barred bandicoots (Perameles gunnii; hereafter ‘bandicoot’) are extinct on mainland Australia due to habitat loss and introduced predators, and are the focus of a long-term captive breeding and reintroduction program. Our trials showed that captive bandicoots failed to recognise cat (Felis catus) scents as belonging to a predator, suggesting prey naivety towards cats. We trialled five stimuli to elicit short-term fear behaviour in bandicoots. An automatic compressed air spray and automatic bin lid were most effective. We coupled these stimuli with cat urine during predator aversion training and presented them to bandicoots on three occasions. Bandicoots learnt to avoid the area containing cat urine, suggesting bandicoots are capable of learning new behaviours rapidly. Six trained and five untrained captive bandicoots where released onto Summerland Peninsular, Phillip Island (with cat densities at 1.1 cats/km2). Both had high survival and recapture rates 7 months after release. Training endangered species to avoid introduced predators could assist with long-term species recovery.

Spatial and temporal interactions between endangered spotted‐tailed quolls and introduced red foxes in a fragmented landscape

AbstractHabitat fragmentation can have detrimental impacts on native predators globally through the loss of habitat and associated impacts from introduced predators.The endangered spotted‐tailed quoll (Dasyurus maculatus) is the largest marsupial carnivore on mainland Australia and is sympatric with an introduced predator, the red fox (Vulpes vulpes). Spotted‐tailed quolls are considered a forest‐dependent species and are often associated with large, intact forested habitats where abundance of prey is high and competition with foxes is low. Spotted‐tailed quolls are known to persist in some fragmented habitats in sympatry with foxes; however, the mechanisms facilitating this coexistence are unclear. For 15 months in 2018–19, we used camera traps to investigate whether coexistence between quolls and foxes in a fragmented landscape was facilitated by spatial and/or temporal separation of activity. We found no evidence of spatial separation, with quolls detected on the majority of cameras where foxes were detected. There was considerable temporal overlap between quolls and foxes (Δ1 = 0.71–0.81) and no evidence that fox presence influenced the temporal activity of quolls (Δ1 = 0.76–0.80). Furthermore, there was no evidence of within‐night spatiotemporal avoidance between quolls and foxes (R2 < 0.01). Our findings suggest that quolls do not offset their spatial and temporal activity to avoid foxes in this fragmented landscape. The spatial and temporal sympatry between quolls and foxes is possibly facilitated by low fox density at this site, suggested by low fox detections. The lack of separation between quoll and fox activity could also explain why quolls become locally extinct in other fragmented landscapes because quolls may not modify their activity to avoid foxes. Future research should focus on investigating quoll and fox interactions along a gradient of fox densities and assess if higher fox densities influence spatial and temporal coexistence with quolls in fragmented landscapes.

Whole of Community Facilitators: An Exemplar for Supporting Rural Health Workforce Recruitment through Students' Professional Experience Placements.

The Whole of Community Facilitator model provides support for healthcare students’ professional experience placements (PEP) in rural regions in Tasmania. In Tasmania, rural PEP is challenged as healthcare facilities are often small and have limited capacity for staff to devote considerable time to supervising students during PEP. Recruitment and retention of the rural health workforce in Tasmania is sometimes difficult because the island State is geographically distant from mainland Australia, and predominantly classified as a regional, rural, or remote area. The University of Tasmania, College of Health and Medicine (the College) explored various initiatives to support rural workforce sustainability, and the project discussed addresses this issue by promoting rural healthcare facilities as potential employment destinations for students upon completion of their course. The model supports the delivery of high-quality supervision to students whilst undertaking rural PEP, to foster positive experiences and potentially influence their future career choices. A successful exemplar was trialled in 2012 and implemented statewide in 2017 using a Whole of Community Facilitation (WOCF) model. The initiative supports host facilities, supervisors, host staff, and students and promotes positive placement experiences. The initiative was designed in consideration of Tasmania’s rurality, and uses a flexible and responsive framework.

Reintroduction of Bilbies (Macrotis lagotis) to Matuwa, an Indigenous Protected Area in Western Australia

The greater bilby (Macrotis lagotis) once occupied much of Australia's mainland. Bilbies are now listed as vulnerable and only occur in 20% of their former range. Operation Rangeland Restoration aims to to restore an ex−pastoral lease; reintroduce several species of locally extirpated fauna, including the bilby; and maintain the area in perpetuity for the conservation of Australian arid zone species. Bilbies were reintroduced to the Matuwa Indigenous Protected Area between 2007 and 2010 and, with ongoing landscape-scale control of feral predators, herbivores, and fire, have thrived. Here, we present a detailed account of the methods used during the reintroduction, showing that between 2007 and 2019 there has been an 88% increase in the area of occupancy by bilbies at Matuwa. The results of 2-ha track plot surveys conducted by the traditional owners of Matuwa suggest that the reintroduced bilbies are emigrating out of Matuwa. In addition, in 2018 and 2019 we used 120 camera-traps over 18 mo and occupancy analysis to confirm the widespread presence of bilbies across Matuwa and define significant habitat correlates. Bilbies were more likely to be detected on sandplains with Eucalyptus species as overstorey vegetation and Triodia as understorey vegetation. Bilbies were not detected in habitats with ≥ 75% bare ground. We attribute the success of the bilby reintroduction at Matuwa to the consistent implementation of landscape-scale control of feral predators.

A record of fallout 239Pu and 240Pu at World Heritage Bathurst Harbour, Tasmania, Australia

This study presents the first measurements of anthropogenic plutonium (239Pu and 240Pu) concentrations and atom ratios (240Pu/239Pu) for Tasmania, in sediment collected from Bathurst Harbour, in the Tasmanian Wilderness World Heritage Area, Australia. The weighted mean 240Pu/239Pu atom ratio measured at this site was 0.172 ± 0.007 which is consistent with published data from mainland Australia and global and Southern Hemisphere averages. The 240Pu/239Pu atom ratios ranged between 0.11 and 0.21 with the earliest recorded 240Pu/239Pu atom ratios being the lowest, suggesting an influence of low atom ratio fallout from nuclear testing in Australia. Post-moratorium fallout 240Pu/239Pu atom ratios were consistent with other records. Lead-210 (210Pb) sediment chronologies indicate sediment accumulation rates have increased since the early part of the 19th century at this location.

Portable X-ray fluorescence for bone lead measurements of Australian eagles

Lead (Pb) toxicity from ammunition has been shown to be a threat to scavenging birds across the globe. Toxic levels of lead have recently been found in Australia's largest bird of prey, the wedge-tailed eagle (Aquila audax), through inductively coupled plasma mass spectrometry (ICP-MS) analysis of liver and bone samples. However, ICP-MS is consumptive (causing damage to archived specimens), time-consuming, and expensive. For these reasons, portable X-ray fluorescence (XRF) devices have been optimized to measure bone lead in North American avian species, humans, and other environmental samples. In this study, we assessed portable XRF for bone lead measurement in Australian raptors in two parts. First, we validated the method using tissues from wedge-tailed eagles from Tasmania (A. a. fleayi), analysing bone samples taken from sites on the femur immediately adjacent to sites for which we had ICP-MS data (n = 89). Second, we measured lead via portable XRF in the skulls of wedge-tailed eagles from south-eastern mainland Australia (A. a. audax) collected during a criminal prosecution (n = 92). Portable XRF bone lead measurement demonstrated an excellent correlation with ICP-MS results using root-transformed regression (R2 = 0.88). Calculated equivalent ICP-MS values revealed that greater than 50% of the eagles from mainland Australia had elevated lead levels (>10 mg/kg) and 13% had severe lead exposure (>20 mg/kg). Our results support previous studies of North American avian species and suggest that portable XRF could be a useful and inexpensive option for measurement of bone lead in Australian scavenger species.

Governing in a Pandemic: Law and Government in Australia, 1919

The 1918–19 pneumonic influenza pandemic reached into mainland Australia in January 1919 and spread through the country over the next nine months. As a major public health emergency the pandemic proved to be a significant test of Australian governance. This article explores this history through attention to the imperial framing of Australian international and regional responses, the mechanisms of federal-state relations, the constitutional framework for management of domestic health and public order, and the legal disposition of rights and responsibilities as they arose in the events of 1919. The experience of 1919 highlighted institutional strengths of the Australian state by this period, in constitutional and governmental arrangements, and in capabilities of domestic management of health and public order.

Adhesive Droplets of Glowworm Snares (Keroplatidae: Arachnocampa spp.) Are a Complex Mix of Organic Compounds

Adhesive snares built from silks are fascinating adaptations that have rarely evolved outside spiders. Glowworms (Arachnocampa spp.) are an iconic part of the fauna of Australia and New Zealand that combine the construction of a sticky snare with a bioluminescent lure. Recently, the structure and biomechanical properties of glowworm silk have been studied in detail, but the chemical composition of its adhesive coating, and how it varies between species of Arachnocampa remained unclear, limiting an understanding of the glue function. Here, we studied the chemical composition of the water-soluble fraction of the adhesive droplets from the snares in cave and epigaeic populations of three species of Arachnocampa from mainland Australia, Tasmania, and New Zealand, using a combination of nuclear magnetic resonance and mass spectrometry. We found that glowworm glues comprise a large variety of small organic compounds, with organic acids, amino acids, amino acid derivates, alcohols, urea, and urea derivates being the major fraction, supplemented by small amounts of sugars, fatty acids, and other organic compounds. While there was a general overlap in the compounds detected in the adhesives of all tested Arachnocampa species and populations, the relative amounts differed considerably. We expect that these differences are a product of diet rather than an adaptive response to different environments, but experiments are needed for clarification. The high amount of polar substances and compounds that are hygroscopic at high humidity explains the adhesive properties of the viscous solution and its stability in damp environments. These results contribute to our understanding of the unique prey capture strategy of glowworms. Further, the comparison with convergent spider webs highlights the use of small polar compounds as plasticizers of macro-molecular bioadhesives as a general principle. This may inspire the biomimetic design of novel pressure sensitive adhesives with high performance under high humidity conditions.

Two Severe Prolonged Hydrological Droughts Analysis over Mainland Australia Using GRACE Satellite Data

In recent years, many droughts have happened over mainland Australia, especially the two severe prolonged droughts, from 2006 to 2009 and 2018 to 2020, resulting in serious water scarcity. Therefore, using the Total Storage Deficit Index (TSDI) from the Gravity Recovery and Climate Experiment (GRACE), we analyzed the two severe prolonged droughts from the perspective of the affected area, spatial evolution, frequency, severity and drought driving factors. The results show that the affected area of Drought 2006–2009 ranged from 57% to 95%, and that of Drought 2018–2020 ranged from 45% to 95%. Drought 2006–2009 took its rise in southeastern Australia and gradually spread to the central part. Drought 2018–2020 originated in the southwest corner of the Northern Territory and northern New South Wales, and gradually expanded to Western Australia and the whole New South Wales respectively. During Drought 2006–2009, Victoria suffered drought all months, including 59% mild drought and 41% moderate drought, North Territory had the highest drought severity of 44.26 and Victoria ranked the second high with the severity of 35.51 (cm months). For Drought 2018–2020, Northern Territory was also dominated by drought all months, including 92% mild drought and 8% moderate drought, the drought severities were in North Territory and Western Australia with 52.19 and 31.44 (cm months), respectively. Finally, the correlation coefficients between the two droughts and Indo-Pacific climate variability including El Niño-Southern Oscillation and Indian Ocean Dipole (IOD) are computed. By comparing the correlation coefficients of Drought 2018–2020 with Drought 2006–2009, we find that the impact of the El Niño on the hydrological drought becomes weaker while IOD is stronger, and the role of Southern Oscillation on droughts is diverse with the quite different spatial patterns. The results from Fourier analysis confirm that the two hydrological droughts are all related to Indo-Pacific climate variability but with slightly different driving mechanisms.

Complex histories of gene flow and a mitochondrial capture event in a nonsister pair of birds.

Hybridization, introgression, and reciprocal gene flow during speciation, specifically the generation of mitonuclear discordance, are increasingly observed as parts of the speciation process. Genomic approaches provide insight into where, when, and how adaptation operates during and after speciation and can measure historical and modern introgression. Whether adaptive or neutral in origin, hybridization can cause mitonuclear discordance by placing the mitochondrial genome of one species (or population) in the nuclear background of another species. The latter, introgressed species may eventually have its own mtDNA replaced or “captured” by other species across its entire geographical range. Intermediate stages in the capture process should be observable. Two nonsister species of Australasian monarch‐flycatchers, Spectacled Monarch (Symposiachrus trivirgatus) mostly of Australia and Indonesia and Spot‐winged Monarch (S. guttula) of New Guinea, present an opportunity to observe this process. We analysed thousands of single nucleotide polymorphisms (SNPs) derived from ultraconserved elements of all subspecies of both species. Mitochondrial DNA sequences of Australian populations of S. trivirgatus form two paraphyletic clades, one being sister to and presumably introgressed by S. guttula despite little nuclear signal of introgression. Population genetic analyses (e.g., tests for modern and historical gene flow and selection) support at least one historical gene flow event between S. guttula and Australian S. trivirgatus. We also uncovered introgression from the Maluku Islands subspecies of S. trivirgatus into an island population of S. guttula, resulting in apparent nuclear paraphyly. We find that neutral demographic processes, not adaptive introgression, are the most likely cause of these complex population histories. We suggest that a Pleistocene extinction of S. guttula from mainland Australia resulted from range expansion by S. trivirgatus.

Isolation and Characterization of Cross-Reactive Human Monoclonal Antibodies That Potently Neutralize Australian Bat Lyssavirus Variants and Other Phylogroup 1 Lyssaviruses.

Australian bat lyssavirus (ABLV) is a rhabdovirus that circulates in four species of pteropid bats (ABLVp) and the yellow-bellied sheath-tailed bat (ABLVs) in mainland Australia. In the three confirmed human cases of ABLV, rabies illness preceded fatality. As with rabies virus (RABV), post-exposure prophylaxis (PEP) for potential ABLV infections consists of wound cleansing, administration of the rabies vaccine and injection of rabies immunoglobulin (RIG) proximal to the wound. Despite the efficacy of PEP, the inaccessibility of human RIG (HRIG) in the developing world and the high immunogenicity of equine RIG (ERIG) has led to consideration of human monoclonal antibodies (hmAbs) as a passive immunization option that offers enhanced safety and specificity. Using a recombinant vesicular stomatitis virus (rVSV) expressing the glycoprotein (G) protein of ABLVs and phage display, we identified two hmAbs, A6 and F11, which completely neutralize ABLVs/ABLVp, and RABV at concentrations ranging from 0.39 and 6.25 µg/mL and 0.19 and 0.39 µg/mL respectively. A6 and F11 recognize overlapping epitopes in the lyssavirus G protein, effectively neutralizing phylogroup 1 lyssaviruses, while having little effect on phylogroup 2 and non-grouped diverse lyssaviruses. These results suggest that A6 and F11 could be effective therapeutic and diagnostic tools for phylogroup 1 lyssavirus infections.

Revision of the Australopapuan and West Pacific species of plain pumpkin-beetles, the Aulacophora indica species-complex (Coleoptera: Chrysomelidae: Galerucinae).

The 'plain pumpkin-beetle', indica species-complex of Aulacophora Chevrolat, 1836, is revised for Australopapua, Timor and the West Pacific. The species-complex is defined and the following eight included species are diagnosed and described: A. abdominalis (Fabricius, 1781); A. barrogae Reid, Halling Beatson, sp. nov.; A. cornuta Baly, 1879; A. indica (Gmelin, 1790); A. mbabaram Reid, Halling Beatson, sp. nov.; A. relicta (Boisduval, 1835); A. wallacii Baly, 1886; A. wilsoni Baly, 1888. The three other similar species in the region of interest, A. bicolor (Weber, 1801), A coffeae (Weber, 1801) and A. deplanchei (Perroud Montrouzier, 1864), are diagnosed from the Aulacophora indica species-complex and a key is provided for all 11 species. New synonyms are noted as follows (senior synonym first): A. abdominalis (Fabricius, 1781) = A. argyrogaster (Montrouzier, 1861) syn. nov., = A. fabricii Baly, 1886 syn. nov., = A. armigera Baly, 1889 syn. nov., = A. aruensis (Weise, 1892) syn. nov., = A. fauveli Beenen, 2008 syn. nov.; A. relicta (Boisduval, 1835) = A. palmerstoni Blackburn, 1888 syn. nov., = A. imberbis Weise, 1916 syn. nov.. Aulacophora relicta (stat. rev.) is revalidated from synonymy with A. abdominalis and A. wilsoni (stat. rev.) is revalidated from synonymy with A. scutellata (Boisduval 1835). Aulacophora flava Olivier, 1808, is not an available species name. The synonymy of A. robusta Duvivier, 1884 with A. cornuta is confirmed. Five species traditionally placed in Aulacophora, Galleruca flaveola Boisduval, 1835, G. punctata Boisduval, 1835, G. scutellata Boisduval, 1835, Galleruca flavescens Montrouzier, 1856 and Galleruca artensis Montrouzier, 1861, are not identifiable to genus or species and are therefore listed as nomina dubia within Galerucinae. The previously designated lectotype of A. abdominalis is shown to be invalid. Neotypes are created for A. abdominalis and A. relicta as both lack original type material. A lectotype is designated for A. palmerstoni. The plain pumpkin-beetle species are distributed as follows: A. barrogae, A. mbabaram, A. relicta and A. wilsoni are endemic to Australia; A. abdominalis is widespread in the southwestern Pacific and Melanesia, west to Timor and east to Niue, but is absent from mainland Australia; A. cornuta is widespread from eastern India to Melanesia as far east as Guadalcanal; A. indica is widespread from India southeast to Timor and northeast to Guam, and adventitious further south and east, but absent from, or not established in, Australia, Fiji, New Caledonia, Niue, Samoa, Tonga and Vanuatu; A. wallacii is endemic to Timor. Native hosts are unknown for any species, but A. abdominalis, A. indica, A. relicta and A. wallacii are pests of exotic cucurbit crops.