Main Traits Research Articles

GWAS Combined with WGCNA of Transcriptome and Metabolome to Excavate Key Candidate Genes for Rice Anaerobic Germination

Direct seeding of rice is a lightweight and simple cultivation method, which can effectively promote rice production. Anaerobic germination tolerance is one of the main traits of rice adaptability to direct seeding. The mining of related genetic loci, analysis of anaerobic traits and screening of tolerance genes provided valuable genetic resources for improving the anaerobic germination ability of direct seeding rice. This study conducted a dynamic genome-wide association study (GWAS) based on coleoptile-related traits of 591 rice natural populations, and a total of 317 SNP sites were detected. Integrated dynamic widely targeted metabolomics analysis, we found that xanthine, l-alanine and GABA may be key biomarkers that are sensitive and respond strongly to hypoxic stress perception. By WGCNA analysis of targeted metabolomics and transcriptomics, a total of 3 modules were obtained that were significantly correlated with the above three marker metabolites, namely dark green, dark gray and light green modules, respectively, and several key structural genes of OsAlaAT1, OsGAD4, OsAAH and Os09g0424600 that may affect hypoxic germination were screened from the 3 modules. Among them, OsAlaAT1 (Os10g0390500), located in Chr10-12877840, which is within the GWAS location range of CVAN3d, is considered to be a more reliable candidate gene. Overall, in addition to providing new insight into the metabolic regulation of l-alanine, GABA and xanthine during hypoxic germination of rice. This study also provided a reference for the basic theoretical research and breeding application research on the related traits of anaerobic germination in direct-seeding rice.

Moving towards a comprehensive view of the spatial processes in seed dispersal networks: Embracing metacommunities.

The current biodiversity crisis requires efficient approaches to address the ongoing impoverishment of natural communities and the depletion of ecosystem services and functions. In this sense, identifying key species that promote the functioning of ecological processes can be strategic to guide actions aiming at the conservation and restoration of biodiversity. Node-level metrics in interaction networks can be helpful to identify those key species, as they measure the role each species plays in organizing the interactions. Moreover, ecological correlates of species structural roles may vary between local and global networks of interactions, reflecting distinct mechanisms acting at different spatial scales. By studying local seed dispersal networks and one global meta-network combining those local networks, Moulatlet et al. identified the most important traits explaining bird species centrality at varying spatial scales. They found that body mass was the main trait explaining centrality at the local scale, whereas range size was the main predictor of species centrality at the global scale. In this contribution, besides assessing local interaction networks, Moulatlet et al. adopt a biogeographical perspective to seed dispersal systems, extending our knowledge about the possible mechanisms that underlie the organization of interacting assemblages when changing the spatial scale of observation. Future efforts on this field could include an intermediate scale, comprising the level of metacommunities, shedding light on the interplay between local and spatial processes, both embedded in biogeographical realms, when determining the organization of interactions and the ecological correlates of species roles.

Climatic variability and plant functional traits in tropical montane cloud forest refugia along the Trans Mexican Volcanic Belt

Functional traits represent the adaptive responses of plant species to climatic changes. They are an integrative marker of the general environmental conditions at a location. Testing their patterns may help to understand how the functional traits would track these environmental changes of tropical montane cloud forests (TMCFs). These forests are characterized by the constant presence of clouds, and these are among the most threatened ecosystems by climate change worldwide. One of the main TMCF regions in Mexico is the Trans-Mexican Volcanic Belt (TMVB). This region has provided refugia during past climate-change events. We identify three current regions of distribution of TMCF in TMVB, Pacific, Center and Gulf. We used 60 years of climatic information, functional traits and plants databases. We proposed the following aims: to determine whether changes in climatic variation have occurred in the TMVB and how these changes can be related to functional trait variations for dominant tree species across the three TMCF regions, and to know the potential changes as a refuge of the TMCF of the TMVB. We found different rainfall and maximum temperature patterns among and within regions. We identified that the Pacific region forests were characterized by the lowest specific leaf area (SLA), wider leaf thickness and high leaf density, which indicates resources-conservative leaf economics and tolerance to low humidity. Center and Gulf forests were distinguished by the highest SLA, less leaf thickness, and higher leaf density values, which suggests that the leaves of dominant tree species tend to be resources-acquisitive rather than conservative-resources. Finally functional traits were associated with prevailing climatic conditions and suggested that SLA, leaf thickness and leaf density are the main functional traits that could be used to relate them to regional climate. Also, Central and Gulf regions could serve as a refuge for those species of trees demanding humidity.

Genome resequencing reveals the evolutionary history of garlic reproduction traits.

The propagation of cultivated garlic relies on vegetative cloves, thus flowers become non-essential for reproduction in this species, driving the evolution of reproductive feature-derived traits. To obtain insights into the evolutionary alteration of reproductive traits in the clonally propagated garlic, the evolutionary histories of two main reproduction-related traits, bolting and flower differentiation, were explored by genome analyses using 134 accessions displaying wide diversity in these two traits. Resequencing identified 272.8 million variations in the garlic genome, 198.0 million of which represent novel variants. Population analysis identified five garlic groups that have evolved into two clades. Gene expression, single-cell transcriptome sequencing, and genome-wide trait association analyses have identified numerous candidates that correlate with reproductive transition and flower development, some of which display distinct selection signatures. Selective forces acting on the B-box zinc finger protein-encoding Asa2G00291.1, the global transcription factor group E protein-encoding Asa5G01527.1, and VERNALIZATION INSENSITIVE 3-like Asa3G03399.1 appear to be representative of the evolution of garlic bolting. Plenty of novel genomic variations and trait-related candidates represent valuable resources for biological studies of garlic. Numerous selective signatures from genes associated with the two chosen reproductive traits provide important insights into the evolutionary history of reproduction in this clonally propagated crop.

The Role Brand Personality Plays in Purchase Decisions

This research paper investigates the role of Brand Personality in Purchase Decisions in the Indian context. The study explores the perceptions of brand personality traits, their impact on purchase intentions, and the mediating role of emotional responses. A sample of 500 respondents from various age groups in India participated in the study. The research findings reveal five main brand personality traits perceived by consumers: sincerity, excitement, competence, sophistication, and ruggedness. Among these, sincerity, excitement, and competence demonstrate a significant positive correlation with purchase intentions, indicating their influential role in shaping consumer behaviour. The study further identifies emotional responses, particularly joy and trust, as mediators in the brand personality-purchase intention relationship. Moreover, age is found to moderate the impact of brand personality on purchase decisions, suggesting differences in perceptions among different age groups. These findings have significant theoretical implications for the understanding of brand personality and consumer behaviour in the Indian market. Marketers can leverage the insights to tailor branding strategies, evoke positive emotional connections, and enhance purchase intentions among diverse consumer segments.

Trade-offs among leaf toughness, constitutive chemical defense, and growth rates in oaks are influenced by the level of leaf mass per area

Key messageAmong the variety of leaf defensive strategies to counteract herbivory attacks, the oak species analyzed in this study maximize investment in no more than one, with high-LMA oaks developing very tough leaves and low-LMA oaks favoring between high concentrations of condensed tannins or high growth rates.ContextPlants develop a variety of defense strategies to counteract herbivory attacks, from physical and chemical defenses to tolerance strategies. Tradeoffs between strategies have been widely assessed from a resource allocation perspective, but there is a need to consider eventual interactions among them.AimWe evaluate the among-species tradeoff between three main constitutive plant defense traits, while considering the leaf mass per area ratio (LMA) as a proxy of leaf construction investment on area basis.MethodsLeaf toughness measured as work of fracture, condensed tannins, and relative growth rate (RGR) were analyzed in a set of 19 Quercus L. species with contrasting LMA.ResultsMost species had low values either in two or in the three traits analyzed. Moreover, the highest values of work of fracture appeared in the species with high LMA; the highest values of condensed tannins were found in the species with the lowest LMA; and high values of RGR were measured in species with intermediate or lower values of LMA.ConclusionOaks showed a trade-off among leaf defensive strategies influenced by LMA. Oaks with high LMA developed very tough leaves while oaks with low LMA presented lower values of toughness but favored between one of the two other strategies.

The Motherland is Unique, the Poet is Unique

The main trait of Hamid Olimjon’s poetry is to absorb the feelings to people namely living with full of joy and happiness. The prime example of this considered to be the poetry “Uzbekistan”. The infinite feelings about reality, beauty, and outstanding emotions are described in this poetry.

Terminal-based zonal bus: A novel flexible transit system design and implementation

The pursuit of individual mobilities expedites the development of demand-responsive systems, while the balance between service flexibilities and operational cost is becoming particularly important. This paper proposes a method for operating a novel demand-responsive bus system termed terminal-based zonal bus (TZB). It is a flexible system with vehicles executing a terminal-based zone routing scheme in specified service zones. The terminal-based routing scheme intends to generate TZB lines, and each line is operated by one vehicle that travels along a series of terminal stations arranged in order and serves enroute passenger requests between any two consecutive terminal stations. As instant response is the main trait of demand-responsive systems, TZB is able to address instance response by incorporating reserve capacity, which is employed to indicate the vehicle responsive ability of new real-time requests. Further, a tailored heuristic algorithm is designed to solve the proposed model, which simultaneously incorporates several practical restrictions, including the capability of adopting real-time requests, terminal stations’ fleet-size constraints, time window constraints, and vehicle capacity. Computational experiments are conducted based upon the newly developed modern city Xiong An, China which confirm that the proposed method is capable of obtaining high-quality solutions efficiently, and TZB can provide flexible service at a relatively low operational cost.

Spatio‐temporal species aggregations do not rule out interspecific competition in tree hollow spider assemblages

Abstract A community's biological diversity reflects coexistence between species. This often depends, to some extent, on whether there is competition for resources and how it is dealt with. The nature of old‐growth tree hollows is confined and relatively isolated, which makes them ideal for investigating the competition phenomena between their inhabitants, such as Araneae. The existence of interspecific competition in the structuring of tree hollow spider assemblages in Mediterranean forests was inferred by spatial co‐occurrence pattern analyses with null models at both the community and pairwise levels. The analysis included 36 spider species collected monthly with emergence traps for 1 year. The distribution of four ecological traits, body size and phenology on the resulting pattern type was discussed. The analyses showed spatial segregation at the community level and spatial aggregations between species with different traits predominated at the pairwise level. Hunting strategy and body size were the main differential traits to facilitate these aggregations. In addition, only the aggregations led by Amaurobius scopolii–Scotophaeus scutulatus and Eratigena atrica–Liocranum majus also showed an overlap during their main activity period. Community segregation and spatio‐temporal aggregations of species with differential traits suggest that interspecific competition is a very likely structuring factor of tree hollow spider assemblages. Instead, segregations at the pairwise level seem to result from other factors, for example, habitat preferences.

Influence of Incremental Short Term Salt Stress at the Seedling Stage on Root Plasticity, Shoot Thermal Profile and Ion Homeostasis in Contrasting Wheat Genotypes

Understanding the component traits determining salt stress tolerance is a major breeding target in wheat. The lack of genetic resources suited to salt-affected regions and the complexity of the traits involved impede progress in breeding salt-tolerant wheat varieties. This study was conducted with four bread wheats, namely (Triticum aestivum) Kharchia-65 (K-65), BT-Schomburgk (BTS), HD-2687, and HD-3298. Treatments were imposed on plants with varying electrical conductivity (control, 5 dS m−1, 10 dS m−1, and 15 dS m−1) with a combination of three different salts NaCl, CaCl2·2H2O, and Na2SO4. We evaluated variations in root system architecture, canopy temperature (depicted as a thermal image), reactive oxygen species (ROS) homeostasis, and leaf stomatal density in response to incremental doses of salt stress in a hydroponic experiment. As the plants were sampled after short-term exposure to stress (within 3 weeks of stress imposition), the plants were expected to be in a quiescent state. Due to the osmotic effect, the growth of the plants was compromised, and the associated decrease in stomatal conductance increased the canopy temperature. ROS accumulation and antioxidant enzyme activity did not follow a definite pattern. The antioxidant system’s tolerance to ROS comes into action much later in the tolerance mechanism. That could probably be the reason behind the varied response in ROS accumulation and antioxidant enzymes after short-term exposure to salt stress. Thermal images could effectively differentiate between salt-tolerant (K65) and sensitive (HD2687) genotypes. The variation in Na+/K+ ratio also suggested a genotypic variation in salt tolerance. The genotypes of K-65 maintained a better root system, while HD2687 showed severe reduction in root biomass and other root traits under salt stress. The PCA data also point out genotypic variation in lateral and main root traits in response to different salt stress levels. For salt tolerance in wheat, the main contributing root traits were total root length, total surface area, total root volume, tips, and other main, lateral root traits. The idea of differential control of RSA dynamics is novel and can be further explored to understand natural variation in salt stress tolerance.

Exploring the morphology of adult tibia and fibula from Sima de los Huesos site in sierra de Atapuerca, Burgos, Spain.

The analysis of the locomotor anatomy of Late Pleistocene Homo has largely focused on changes in proximal femur and pelvic morphologies, with much attention centered on the emergence of modern humans. Although much of the focus has been on changes in the proximal femur, some research has also been conducted on tibiae and, to a lesser extent, fibulae. With this in mind, we present one of the largest samples of the same population of human tibiae and fibulae from the Middle Pleistocene to determine their main characteristic traits and establish similarities and differences, primarily with those of Neanderthals and modern humans, but also with other Middle Pleistocene specimens in the fossil record. Through this study, we established that the Middle Pleistocene population from the Sima de los Huesos (Atapuerca, Burgos, Spain) had lower leg long bones similar to those of Neanderthals, although there were some important differences, such as bone length, which this fossil individuals resembled those of modern humans and not to Neanderthals. This fact is related to the crural index and leg length, even though we do not have any true association between femora and tibiae yet, it has implications for establishing locomotor efficiency and climate adaptation.

BSA-Seq for the Identification of Major Genes for EPN in Rice.

Improving rice yield is one of the most important food issues internationally. It is an undeniable goal of rice breeding, and the effective panicle number (EPN) is a key factor determining rice yield. Increasing the EPN in rice is a major way to increase rice yield. Currently, the main quantitative trait locus (QTL) for EPN in rice is limited, and there is also limited research on the gene for EPN in rice. Therefore, the excavation and analysis of major genes related to EPN in rice is of great significance for molecular breeding and yield improvement. This study used japonica rice varieties Dongfu 114 and Longyang 11 to construct an F5 population consisting of 309 individual plants. Two extreme phenotypic pools were constructed by identifying the EPN of the population, and QTL-seq analysis was performed to obtain three main effective QTL intervals for EPN. This analysis also helped to screen out 34 candidate genes. Then, EPN time expression pattern analysis was performed on these 34 genes to screen out six candidate genes with higher expression levels. Using a 3K database to perform haplotype analysis on these six genes, we selected haplotypes with significant differences in EPN. Finally, five candidate genes related to EPN were obtained.

Agro-biological Characteristics and Biochemical Parameters of Purple Waxy Corn [Zea mays (L.) var certaina] in Son La Province, Vietnam

Background: Local purple waxy corns, biocompound activity and nutrition content good for health, are growing from different districts and altitude of sea level in Sõn La province with various characters. It is essential for evaluation of agro-biological characteristics and nutritional composition of local purple waxy corns was to find out which is the best and suitable for rejuvenlination, market and production in Son La province. Methods: Field experiment was conducted following scientific principle of agricultural research and designed with random completed block design (RCBD) with three replications, a 20 m2 block area. Observations and quality analysis of main traits of 12 purple local waxy corns were analyzed in summer season of 2022. Result: Results showed various data of agro-biological characters, yield and biochemical kernel characters of twelve local purple waxy corns. SM8 local purple waxy corn variety showed the best agro-biological characters such as soluble protein 6.25 mg/g, total phenol 4.12 mg/g, chlorophyll stable index 89.5%, yield about 3.96 tons/ha, quality grain with starch content 68.63%, protein content 8.53% and anthocyanin content 315.03 mg/100 g. This variety can be a good option for the farmers to produce a variety with higher yield and good agro-biological characteristics.

Psychological Aspect of Legal Impact

The paper considers the phenomenon of legal impact as a socio-psychological legal phenomenon. The subject of law is in the core of the epistemological analysis of the psychological aspect of legal impact. The subject is the only component able to actively respond to legal impact. An active reaction is expressed as participation in legal impact, obtaining and processing legal information, modeling one’s own behavior in accordance with the information received. In the paper, the author reviews some research in this field and concludes that there is a need to strengthen interdisciplinary interaction between the theory of law and psychological sciences in a quantitative and qualitative way. The absence of such studies determines the relevance of this study. The main personality traits determining the strategy of its behavior, the influence of external and internal factors are determined. In the system of social communication, a person is an active self-organizing entity that transmits and transits legal information to subjects of other levels of social interaction. The assessment of the significance of legal information and the choice of a decision on its use is carried out by the subject independently, using their own social experience and psychological properties. The mechanism of psychological legal impact has been studied. Within the framework of the paper, the author substantiates the need to introduce the concept of «directed behavior» into scientific circulation, which should be understood as the state of the subject experiencing legal influence, as well as the consequences in the form of certain acts of the subject mediated by legal impact, the process of exchange of significant legal information between participants in social communication. The role of legal awareness and legal information in the mechanism of psychological legal impact is determined.

GWAS with principal component analysis identify QTLs associated with main peanut flavor-related traits.

Peanut flavor is a complex and important trait affected by raw material and processing technology owing to its significant impact on consumer preference. In this research, principal component analysis (PCA) on 33 representative traits associated with flavor revealed that total sugars, sucrose, and total tocopherols provided more information related to peanut flavor. Genome-wide association studies (GWAS) using 102 U.S. peanut mini-core accessions were performed to study associations between 12,526 single nucleotide polymorphic (SNP) markers and the three traits. A total of 7 and 22 significant quantitative trait loci (QTLs) were identified to be significantly associated with total sugars and sucrose, respectively. Among these QTLs, four and eight candidate genes for the two traits were mined. In addition, two and five stable QTLs were identified for total sugars and sucrose in both years separately. No significant QTLs were detected for total tocopherols. The results from this research provide useful knowledge about the genetic control of peanut flavor, which will aid in clarifying the molecular mechanisms of flavor research in peanuts.

Zajištění informací a věcí v návrhu nového trestního řádu

The paper focuses on an overview of the Draft New Code of Criminal Procedure’s chapter covering searching for, discovering, and securing information, items, electronic data, and documents important for criminal procedure. It is divided into two main parts. The first is a brief introduction to the new conception in a general manner and strives to provide a comprehensive analysis of its main cornerstones and traits, the second consists of an analysis of the most important novelties in particular areas such as a new regime for securing electronic data, new rules for the search of premises where advocacy is conducted, a determination of ownership of a secured item etc. It also brings a discussion of a few chosen elements, which the authors deem as controversial or important. Where the text of the draft is criticized, possible solutions are also recommended.

The effect of extended cold storage on the functional quality attributes of different potato genotypes

AbstractPotato (Solanum tuberosum L.) is a staple food widely consumed in Tunisia and represents a source of various secondary metabolites with human health benefits. However, cold storage of potato tuber affects their bioactive properties and functional quality. Despite the importance of this fact, few studies have examined the effects of extended cold storage on potato tubers quality. Therefore, the objective of the present work was to evaluate the effect of cold storage on the main quality traits of different potato genotypes grown in Tunisia. This investigation focused on four cultivated potato varieties including three commercial (Selena, Elbeidha, and Naima) and a selected clone CL27 grown under late cropping season conditions with planting at the beginning of autumn and a winter tuberization. The tubers from this trial were stored for 90 days at 8°C. The results obtained showed that quality traits of potato tubers were strongly influenced by storage at 8°C throughout 90 days. Additionally, disregarding the genotype, total carotenoids, phenolics, and flavonoids contents increased significantly following 90 days storage at 8°C. However, genotypic differences underlying the antioxidant activity in both hydrophilic and lipophilic fractions were detected suggesting their sensibility to cold storage. This study might be useful for breeders working on potato genotypes suitable for extended storage duration and for growers storing their material for extended period under low temperature.

Use of cytoplasmic male sterility in sorghum breeding for productivity

The productivity increase of many agricultural crops is associated with the production of heterotic hybrid forms that are more productive than conventional varieties. In order to obtain a heterotic effect and develop hybrid seeds in sorghum breeding practice, the phenomenon of cytoplasmic male sterility is used. The purpose of the current work was to study new sorghum-Sudan hybrids developed on the basis of CMS, and to determine heterosis according to the main economically valuable traits that affect green mass productivity. The study was carried out in theAgricultural Research Center “Donskoy” in 2020–2022. The objects of the study were the sorghum-Sudanese hybrids studied in the competitive variety testing. Sowing and field observations were carried out according to generally accepted methods. The meteorological conditions during the years of study (2020–2022) varied significantly, which made it possible to conduct a comprehensive estimation of hybrids in contrasting conditions. According to the study carried out according to the main traits (length of “sprouts-heading stage” period, plant height, length of the 3rd leaf and number of leaves per plant), which affect the productivity of sorghum-Sudan hybrids, there has been established a high heterotic effect. Hybrids developed on the basis of CMS had a green mass productivity of 3557–5949 g/m2 for two cuts. Hypothetical heterosis ranged from 59.8 to 144.3 %, true heterosis was 22.1–95.1 %. According to the green mass productivity, the hybrids ‘APV-1115 x Kudesnitsa’, ‘APV-1115 x Svetloplenchataya 2’, ‘APV-1115 x ChT-22’, ‘Dzhetta x ChT-22’ were the best ones, exceeding the standard on 755–1384 g/m2 or by 16.5–30.3 %. The identified hybrids have confirmed the expediency of using cytoplasmic male sterility to obtain a highly heterotic effect both in terms of green mass productivity and in terms of the main traits that affect productivity.

The spring durum wheat variety ‘Bezenchuksky Podarok’

The purpose of the current study was to determine the main economically valuable traits of the new spring durum wheat variety ‘Bezenchuksky podarok’, to establish its significance in the system of durum wheat varieties and to describe the main approbation traits. The variety was developed by individual selection from the hybrid population F9 746D-39/Pamyati Chekhovicha. Its pedigree includes such varieties as ‘Kharkovskaya 46’, ‘Bezenchuksky yantar’, ‘Saratovskaya zolotistaya’, ‘CIMMYT – Altar 84’, ‘Ahninga’, intermediate breeding lines ‘92D-4’, ‘2125D-4’, ‘746D-18’ of the Samara RIA and the breeding line ‘358s-94’ of the RIA of the South-East. Field trials, phenological observations and estimations in the competitive and ecological variety trials were carried out in accordance with the requirements of the methodology of the State Commission for Variety Testing and Protection of Breeding Achievements. The variety belongs to the Volga steppe ecological group, middle-maturing biotype, having a medium-sized stem (with the height reduction gene RhtAhng). Over the years of study (2018–2022), in the competitive variety testing, a new variety with a mean grain productivity of 2.36 t/ha exceeded the standard variety ‘Bezenchukskaya 210’ on 0.15 t/ha and the variety ‘Bezenchukskaya zolotistaya’, which is widespread in the Volga region and the Urals, on 0.19 t/ha. In the ecological variety testing in the Samara SAU (Kinel) and in the RIA of the South-East (Saratov), the excess over local standards was 1.05 t/ha and 0.06 t/ha, respectively. In the competitive variety testing (2018–2022), the new variety was the best in its responsiveness to environmental conditions (the regression coefficient bi was 1.06, the standard variety’s coefficient was 1.00), and its resistance to stem rust pathogens and leaf spots (Alternaria sp., Fusarium sp.). The new variety was the best in terms of gluten quality with the parameters of SDS (52 ml), and IG (94.0 %), which makes it possible to attribute it to the class of durum wheat varieties with excellent gluten. Thus, gluten quality and production capabilities of the variety ‘Bezenchuksky Podarok’ have enhanced the diversification of the durum wheat varietal system in the Middle Volga region.

Comprehensive agrobiological assessment and analysis of genetic relationships of promising walnut varieties of the Nikitsky Botanical Gardens.

Walnut is an important horticultural crop, the production of which ranks second among all nut crops. Despite the significant demand in the domestic market in Russia, the industrial production of walnut fruits in Russia is currently underdeveloped. At the same time, there is a need to update the assortment with new highly productive varieties adapted to local agro-climatic conditions and having high quality nuts that are competitive at the world level. An important issue for the successful implementation of breeding programs is a comprehensive study of the gene pool. In this regard, within the framework of the study, the task was to evaluate promising varieties from the collection of the walnut gene pool of the Nikitsky Botanical Gardens and analyze genetic relationships based on microsatellite genotyping. On the basis of the performed phenotypic assessment, the study sample, which included 31 varieties, was divided into several groups according to the main phenotypic traits, such as frost and drought resistance, the start of the growing season, the ripening period, the weight and type of flowering, the weight of the fruit, and the thickness of the endocarp. Varieties with economically valuable traits that can be recommended as promising as initial parental forms in breeding work for resistance to abiotic stress factors have been identified, as well as varieties with increased productivity and large fruit sizes. Based on the analysis of eight SSR markers (WGA001, WGA376, WGA069, WGA276, WGA009, WGA202, WGA089 and WGA054), an analysis of the level of genetic diversity was performed and genetic relationships were established in the studied sample of varieties. Six (for WGA089) to eleven (for WGA276) alleles per locus have been identified. A total of 70 alleles were identified for the eight DNA markers used, with an average value of 8.75. Analysis of SSR genotyping data using Bayesian analysis established the presence of two main groups of genotypes. Taking into account the fact that all the studied varieties are selections from local seed populations in different regions of the Crimean Peninsula, the revealed level of polymorphism may indirectly reflect the level of genetic diversity of the local walnut populations. Furthermore, the presence of two genetically distant groups indicates the presence of two independently formed pools of the autochthonous gene pool of the species Juglans regia L. on the Crimean Peninsula.