An investigation was carried out to assess the gene action in bottle gourd for twenty- three characters at Main Experiment Station, Department of Vegetable Science, A.N.D.U.A.&T., Kumarganj, Ayodhya (U.P). during Zaid 2022 using 12 genotypes, nine lines i.e., Narendra Rashmi, NDBG-17, Pusa Naveen, NDBG-21, Punjab Komal, NDBG-28, NDBG-65-2, NDBG-105, NDBG-Sel-2 and three testers i.e., Narendra Prabha, Narendra Kamna, Narendra Pooja and their 27 hybrids obtained through line × tester mating pattern. The variances due to sca (σ2 s) were higher than variance due to gca (σ2g) for all the characters. The value of dominance genetic variances (σ2 D) were larger than additive genetic variance (σ2 A), indicating the presence of dominance and over dominance effects and preponderance of non-additive gene action .The heritability in narrow sense was low for most of the traits except fruit length, days to first staminate flower anthesis and primary branches per plant where high estimate of heritability in narrow sense with low genetic advance were recorded, indicating the presence of non-additive gene action accompanied by environmental influence.