Main Products Research Articles

Epoxyethane Methoxycarbonylation over a Heterogeneous Catalyst Based on Functionalized Cobalt Carbonyl.

In this work, we report a new heterogeneous catalyst for the methoxycarbonylation of epoxyethane (EO) to methyl 3-hydroxypropanoate (3-HPM), achieving good yields and recyclability of up to three times with no obvious loss in catalytic activity. The catalysts were prepared through an effective strategy to anchor active cobalt carbonyl species to functionalized silica-based molecular sieves. The successful grafting of functional ligand and cobalt carbonyl cluster was evident through 29Si-MAS NMR and FT-IR studies. Furthermore, an in situ FT-IR study indicates that a binuclear cobalt carbonyl catalyst may undergo a shift from bridged carbonyl to terminal carbonyl in the methoxycarbonylation of EO. According to time-dependent in situ FT-IR spectra in the carbonyl stretching region, the main product and intermediate are both captured, showing a new reaction mechanism different from the previous literature reports. These findings are significant for insight into the relation between the catalytic performance and structure.

Ketogenic diet induces an inflammatory reactive astrocytes phenotype reducing glioma growth

The use of a ketogenic diet (KD) in glioma is currently tested as an adjuvant treatment in standard chemotherapy regimens. The metabolic shift induced by the KD leads to the generation of ketone bodies that can influence glioma cells and the surrounding microenvironment, but the mechanisms have not yet been fully elucidated. Here, we investigated the potential involvement of glial cells as mediators of the KD-induced effects on tumor growth and survival rate in glioma-bearing mice. Specifically, we describe that exposing glioma-bearing mice to a KD or to β-hydroxybutyrate (β-HB), one of the main KD metabolic products, reduced glioma growth in vivo, induced a pro-inflammatory phenotype in astrocytes and increased functional glutamate transporters. Moreover, we described increased intracellular basal Ca2+ levels in GL261 glioma cells treated with β-HB or co-cultured with astrocytes. These data suggest that pro-inflammatory astrocytes triggered by β-HB can be beneficial in counteracting glioma proliferation and neuronal excitotoxicity, thus protecting brain parenchyma.

Mechanisms of C(sp3)-H Functionalization of Acetonitrile or Acetone with Alkynes: A DFT Investigation.

The mechanisms for the C(sp3)-H activation and addition reactions between acetonitrile (or acetone) and alkynes have been investigated with the M06-2X-D3/ma-def2-TZVP method and basis set. The SMD (solvation model based on solute electron density) model was applied to simulate the solvent effect. In the first and second reactions, 2-phenylbut-3-yn-2-ol reacted with acetonitrile and acetone, respectively. First, the C(sp3)-H activations of acetonitrile and acetone could be achieved by PhCOO• and t-BuO• radicals. Then, addition reactions converted 2-phenylbut-3-yn-2-ol into final products P1 and P2. Gibbs free energy surfaces of these two reactions suggest that blue lines would be the favorable paths with lower Gibbs energy barriers, and the terminal C atom of the C≡C bond is the best reactive site. Moreover, the analysis of the IRI (Interaction Region Indicator) reveals the Z- and E-configuration transformations. While in the third and fourth reactions, methyl(2-(phenylethynyl)phenyl)sulfane has interactions with acetonitrile and acetone via some paths, respectively. Gibbs free energy profiles show that the C10 atom, rather than the C11 atom, has priority, and the blue lines are favorable. Furthermore, the action mode of Na2HPO4 could reduce the energy barrier and benefit the reaction. vdW (van der Waals) interactions play an important role in the choice for the reactive site. In the fifth (or sixth) reaction, it happened between 1-(2-(methylthio)phenyl)-3-phenylprop-2-yn-1-one and acetontrile (or acetone) to yield the final product P5 (or P6). The computational results uncovered the blue line is the best path, and the choice for the reactive site depends on the vdW interactions, which reveals the origin of selectivity. In addition, the investigation for the byproducts have been carried out, and these can explain the reason that only the main product is produced. Both of those can agree with the experimental results. The localized orbital locator (LOL) isosurfaces, Laplacian bond order (LBO), electron density of the bond critical point (ρBCP), electron spin density isosurface graphs, and IRI graphs can be used to analyze the structure and reveal the reaction substances.

Identification of Wheat Stripe Rust Inoculum Sources and Dispersal Routes Responsible for Initial Rust Establishment in Southern Henan of China.

Wheat stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is an important airborne disease worldwide. Pst inoculum strength in southern Henan in winter or early spring is important for spring epidemics in the main autumn-sown wheat-growing regions of China. However, there is limited knowledge about the source and time of initial infection on winter wheat in southern Henan. The first occurrence of wheat stripe rust in southern Henan was recorded annually from 2011 to 2022, from which we used the backward trajectory approach to infer the likely source of Pst inoculum responsible for the initial disease occurrence. The results suggested that the Pst inoculum responsible for initial rust established in the winter in southern Henan originated from the Gansu Pst oversummering area in China, whereas it originated from the adjacent winter Pst sporulation regions in southern Shaanxi and northwestern Hubei if Pst symptoms were first observed in early spring in southern Henan. Another possible Pst source is southern Hubei where Pst can also sporulate in the winter. Thus, early Pst development in winter in the main wheat production region in China (Henan) is likely to be caused by Pst inoculum spread from the oversummering inocula or Pst epidemics in autumn seedlings in Gansu.

Photooxidation of Dipyrrinones: Reaction with Singlet Oxygen and Characterization of Reaction Intermediates.

Bilirubin (BR) is a water-insoluble product of heme catabolism in mammals. Elevated blood concentrations of BR, especially in the neonatal period, are treated with blue-green light phototherapy. The major mechanism of BR elimination during phototherapy is photoisomerization, while a minor, less studied mechanism of degradation is oxidation. In this work, we studied the oxidation of the bilirubin model tetramethyl-dipyrrinone (Z-13) by singlet oxygen in methanol using UV-vis and ESI-MS spectroscopy, resulting in propentdyopents as the main oxidation products. We also identified two additional intermediates that were formed during the reaction (hydroperoxide 21a and imine 17). The structure of the hydroperoxide was confirmed by helium-tagging IR spectroscopy. Such reaction intermediates formed during the oxidation of BR or bilirubin models have not been described so far. We believe that this work can be used as a first step in studying the complex oxidation mechanism of BR during phototherapy.

Effect of pore throats on the reservoir quality of tight sandstone: A case study of the Yanchang Formation in the Zhidan area, Ordos Basin

Abstract Due to the tight sandstone widespread development of nanoscale pore-throat systems, the microscopic pore-throat characteristics of tight reservoirs are the focus of research. Taking the main tight oil production province of the Ordos Basin in China as a case study, nuclear magnetic resonance (NMR), pressure-controlled mercury injection (PMI), scanning electron microscopy, and micro-computed tomography (μCT) are used to analyze the characteristics of four types of tight sandstones with different pore throats. Furthermore, the effects of pore throats on movable fluid, connectivity, and reservoir physical properties are analyzed. The results show that the pore throats of the four types of tight sandstones are obviously different, but they are all dominated by nanoscale pore-throat systems. From types I to IV, the pore types of the tight sandstone change from residual intergranular pores to the coexistence of feldspar dissolved pores and residual intergranular pores and then to the coexistence of residual intergranular pores and intergranular micropores. The T 2 NMR spectrum changes from a double peak to a single peak, the pore-throat connectivity revealed by μCT decreases from 83.8% for type I to 13.1% for type IV, the pore-throat volume ratio revealed by PMI decreases from 2.09 to 0.43, and the NMR movable fluid saturation decreases from 38.91 to 12.39%. Tight sandstone with larger pores and a uniform distribution has high movable fluid saturation and good pore-throat connectivity. Although the tight sandstone with dissolved pores and intergranular pores may have a medium porosity, the pore–throat connectivity deteriorates compared with high porosity sandstone. Large pore throats, which account for less than 15% of all pore throats, contribute more than 90% of the permeability, while pore throats less than 0.8 μm in diameter are major contributors to the reservoir space, and both the porosity and permeability decrease as the pore-throat diameter decreases.

Comprehensive analysis of ionomic profiling in Chlorella exposed to chlorpyrifos

IntroductionChlorpyrifos (CPF), a widely used organophosphorus insecticide, is highly toxic to non-target aquatic organisms and has relatively high persistence in water, posing a serious threat to marine ecosystems. However, little is known about the toxicological mechanism of CPF on marine microalgae, which is the main primary producer in the marine ecosystem.MethodsThis study explored the ion changes of microalgae Chlorella vulgaris under the stress of CPF through Inductively Coupled Plasma Mass Spectrometry (ICP-MS).ResultsSignificant disparities in ionomics among control and treatment group were observed through pattern recognition analysis (principal component analysis, PCA; orthogonal partial least squares discriminant analysis, OPLS-DA), indicating that CPF may impede their growth by disrupting the homeostasis of crucial elements within algal cells.DiscussionThis study elucidated the inhibitory impact of CPF on green algae growth and its potential mechanism of toxicity through ICP-MS, providing crucial insights for a comprehensive understanding of the influence of organophosphorus pesticides on aquatic ecosystems.

Strategic Priorities for Developing the Marketing of Pomelo Oranges (Citrus maxima Merr.): A Case Study in Magetan Regency, Indonesia

Magetan Regency is the main production center for pomelo in Indonesia with total production reaching 244,395 quintals per year. Despite the high output of pomelo oranges in Magetan Regency, they still need to improve their marketing process. The purpose of this research is to analyze the priority of choosing the best strategy to develop a marketing strategy for pomelo oranges in Magetan Regency. The analysis method uses the Analytic Network Process (ANP) with Super Decision Software. Data collection uses journal reference methods, observations, interviews, and questionnaires to people who are experts in the field of pomelo oranges to obtain data/information regarding criteria, sub-criteria, and alternative solutions that will be analyzed later. The experts are the Head of the Horticulture Division from the Food Crops, Horticulture, Plantation, and Food Security Department, Magetan Regency; pomelo orange farmers in Magetan Regency; and representatives of Field Agricultural Extension Officer Sukomoro District, Magetan Regency. The results of the analysis using the ANP method, lead to the right and best decision in terms of selecting a profitable marketing strategy for pomelo citrus farming in Magetan Regency, namely post-harvest quality improvement. These results can be used as the best alternative because they meet the criteria and sub-criteria above, namely managerial capabilities, customer linking capabilities, market innovation capabilities, human resources assets, and reputational assets.

The Growing Behavior of the Ca2Gd8(SiO4)6O2 Dense Reaction Layer in Molten CMAS at High Temperatures

Calcium–magnesium–alumina–silicate (CMAS), as an environmental deposit, deposits on engine components and causes serious damage to traditional thermal barrier coatings (TBCs) at high temperatures. The rare-earth silicate apatite dense reaction layer is regarded as a promising strategy to prevent TBCs from molten CMAS penetration and corrosion. The interactions between the Gd2O3 ceramic and CMAS are discussed at various temperatures and times in the study. The main reaction products are gadolinium silicate apatite (Ca2Gd8(SiO4)6O2, Gd-apatite) and melilite phases. Within the first 15 min of interaction, a thin, continuous and dense reaction layer (DRL) consisting of Gd-apatite comes to form, and it thickens with increasing exposure temperature and time. The thickness of the DRL is ~0.8 μm after 15 min of the reaction at 1250 °C and it slowly increases to ~9.1 μm after a duration of 24 h at 1400 °C. This is attributed to CMAS infiltration along the grain boundaries of the Gd-apatite phases in the DRL. The growing rates of the Gd-apatite DRL decrease with reaction time and are significantly influenced by the temperature and the ability of the DRL to inhibit CMAS infiltration.

Three-component reactions of conjugated dienes, CH acids and formaldehyde under diffusion mixing conditions

A diffusion mixing technique with a volatile reagent was successfully used to generate crotonic condensation adducts of active methylene compounds and formaldehyde at room temperature in the absence of strong acids and bases. The formed adducts were highly reactive intermediates capable of reacting with dienes in a three-component reaction, leading to the formation of Diels–Alder main reaction products.

The Impact of Composition on the Photoelectrochemical Performance of Molybdenum-Modified Tungsten Oxide in Acidic Media

Abstract The efficiency of photoelectrochemical (PEC) water splitting is considerably controlled by the recombination of photogenerated electron/hole charge carriers at the interface. Herein, the correlation between composition and photoelectrochemical activity is studied by utilizing molybdenum-modified tungsten oxide electrodes. Molybdenum tungsten mixed oxides (Mo x W1−x O3) were synthesized by spray-freeze/freeze-drying approach by varying x from 0 to 1 and studied as a photoanode. The structural changes after Mo substitution in tungsten oxide (Mo x W1−x O3; 0 ≤ x ≤ 1.0) were observed as a function of the composition. In binary oxides, monoclinic structure (ℽ-phase) was observed until Mo substitution (x) reached 0.2. A coexistence of both monoclinic and orthorhombic phases was observed for x varying from 0.2 to 0.8. All synthesized n-semiconducting materials were photoelectrochemically active in water splitting under the acidic condition of HClO4. The highest PEC activity was observed for the sample with low Mo content (x = 0.05) for which the narrowest band gap was determined. The overall activity decrease encountered for Mo-rich materials can be related to a higher tendency to photoinduced proton insertion facilitated by rhombohedral structure. The insight into the mechanism was determined by differential electrochemical mass spectrometry (DEMS). Oxygen (m/z 32) and hydrogen peroxide (m/z 34) were identified as main products. The material with small variation in compositions (x = 0.05) significantly influenced catalytic activity and selectivity, highlighting the importance of the material’s design. Graphical Abstract

Fermentation of sugar kelp (Saccharina latissima): exploring the potential of the kelp’s native microbiota as starter culture and the microbiological food safety of fermented products

This study investigated the use of native microbiota from the sugar kelp Saccharina latissima in the form of fermented kelp fluid (FKF) from a spontaneous anaerobic fermentation process as a starter culture (SC) for the fermentation of freshly harvested biomass of the same species. Rapid (<48 h) acidification (pH < 4.3) was achieved for S. latissima inoculated with FKF, at a fermentation temperature of 21°C. Kelp inoculated with a commercial strain of Lactiplantibacillus plantarum (Lp, positive control) reached a similar pH level after 5 days, while kelp with no SC (negative control) did not reach a pH level below 4.3 within 9 days. The microbiota of the FKF-SC as well as the FKF-inoculated S. latissima samples was dominated by lactic acid bacteria (LAB) identified as L. plantarum. The SC in these samples successfully converted mannitol into lactic acid as the main fermentation product. In contrast, a higher production of acetic acid and ethanol was measured in the negative control samples than in other groups; this reflects a different microbial profile, including marine bacteria which could not be identified by MALDI-TOF biotyping. Challenge trials of S. latissima samples from experimental and commercial fermentation processes with Bacillus cereus did not result in the growth of this food pathogen, even at pH levels within a viable range for this species (pH > 4.3). These preliminary results provide a foundation for further isolation of suitable SCs for kelp fermentation in commercial production and for assessing the food safety of fermented kelp. Efficient and safe fermentation processes will increase sustainability in kelp production and enable a broader use of kelp ingredients in food applications.

Non-canonical NF-κB signaling in dendritic cells is required for ATP-driven indoleamine 2,3-dioxygenase 1 induction through P2Y11 receptor

Abstract Extracellular ATP released from dying cells, including tumor cells, is a key mediator of inflammation and tolerance by binding to purinergic receptors on dendritic cells, resulting in inflammasome activation (via P2X7R), dendritic cell maturation (via P2Y11R), and Indoleamine-2,3-dioxygenase 1 upregulation. However, the regulation of ATP-driven Indoleamine-2,3-dioxygenase 1 expression in human dendritic cells has been poorly investigated. In this work we aimed to investigate the ATP-driven molecular regulation of Indoleamine-2,3-dioxygenase 1 expression via purinergic receptors and to provide an in-depth characterization of ATP-driven T regulatory cells induced by Indoleamine-2,3-dioxygenase 1-expressing dendritic cells. We identified P2Y11R as being responsible for ATP-driven Indoleamine-2,3-dioxygenase 1 upregulation, and non-canonical NF-kB as a molecular pathway associated with ATP-dependent Indoleamine-2,3-dioxygenase 1 induction through P2Y11R. Then we investigated - but did not confirm - an involvement of inflammasome machinery through P2X7R in Indoleamine-2,3-dioxygenase 1 upregulation. Finally, we evaluated the role of ATP catabolism via ATP ectonucleotidases, i.e. CD39 and CD73 and its main product adenosine, in regulating the generation of Indoleamine-2,3-dioxygenase 1-driven T regulatory cells. We found that ATP-driven Indoleamine-2,3-dioxygenase 1 upregulation is associated with CD73 upregulation and adenosine production. Additionally, ATP-treated Indoleamine-2,3-dioxygenase 1-positive mature dendritic cells induce PD-1-expressing bone fide suppressive T regulatory cells via adenosine A2AR. Collectively, a more in-depth understanding of ATP-driven immune-regulatory mechanisms through Indoleamine-2,3-dioxygenase 1 regulation in human dendritic cells leading to the induction of T regulatory cells can have clinical implications for the development of Indoleamine-2,3-dioxygenase 1 inhibitors in cancer patients, especially in combination with immunotherapy such as an anti-CD73 or adenosine receptor agonist and immunogenic chemotherapy.

Analysis of the Spatiotemporal Evolution Patterns and Driving Factors of Various Planting Structures in Henan Province Based on Mixed-Pixel Decomposition Methods

Understanding the spatiotemporal evolution patterns and drivers of cropping structures is crucial for adjusting cropping structure policies, ensuring the sustainability of land resources, and safeguarding food security. However, existing research lacks sub-pixel scale data on planting structure, where planted area data are mainly derived from manual counting results. In this study, remote sensing technology was combined with geostatistical methods to realize the spatiotemporal evolution of crop planting structure at sub-pixel scale. Firstly, the spatial distribution of the multiple cropping structure in Henan Province was extracted based on a mixed-pixel decomposition model, and spatiotemporal evolution of the crop planting structure was analyzed using a combination of Sen’s slope estimator and Mann–Kendall trend analysis, as well as centroid migration. Then, Pearson correlation coefficients were calculated to explore the contribution of driving factors. The results indicate the following: (1) from 2001 to 2022, the cropping structure in Henan Province shows a slightly obvious increase. (2) The centroid of different cropping structures migrates to the main production areas as a whole. (3) Among the driving factors, there was a positive correlation with the labor force and a negative correlation with the urbanization rate. This study provides new insights into the evolution of large-scale crop planting structures and offers significant theoretical and practical value for sustainable agricultural development and the optimization of agricultural planting structures.

Evaluation of the organoleptic characteristics of chocolate made with cocoa beans from three main production areas in Côte d'Ivoire

To evaluate the quality of chocolate produced with Ivorian cocoa beans, the study was carried out in the country's three main production areas (East, Centre-West, and South-West). The project involved collecting data on fermentation times and sourcing samples of cocoa beans from which chocolate had been produced and tasted. The findings of this study indicate that the average fermentation time for cocoa beans is identical across all regions. The observed values (5.30 days in the East, 5.0 days in the Center-West, and 5.04 days in the South-West) are below the recommended 6-day threshold for optimal fermentation. The chocolate produced from the collected beans has been found to have favourable organoleptic characteristics, as evidenced by the low bitterness ratings revealed in the tasting tests. Acidity and astringency are very low and aroma is average. The correlation study between fermentation time and organoleptic characteristics demonstrated that only bitterness (y = -0.2926x + 4.0115; r = 0.62; p < 0.01) and aroma (y = 0.1619x + 2.1324; r = 0.5139; p < 0.01) of chocolate were significantly influenced by fermentation time. Therefore, cocoa from Côte d'Ivoire is suitable for use in the production of high-quality chocolate. However, fermenting the beans for 6 days would be beneficial to achieve a superior quality chocolate product. Int. J. Agril. Res. Innov. Tech. 14(2): 53-61, December 2024

Monitoring Virulence Phenotypes of Bremia lactucae in South and Southeast Brazil

ABSTRACTLettuce downy mildew (Bremia lactucae) is one of the most important diseases of lettuce, especially in conditions of mild temperatures and high humidity. The great variability of B. lactucae makes the use of genetic control complex, as resistance to different virulence phenotypes requires different resistance factors. Dm (“Downy mildew”) genes or resistance factors, have been widely used in lettuce cultivars, providing a high level of resistance to downy mildew. The objective of this work was to monitor and study the virulence phenotypes of B. lactucae in the south and southeast regions of Brazil. Samples with downy mildew symptoms were collected in the main producing areas of the states of São Paulo, Rio de Janeiro, Rio Grande do Sul, Santa Catarina and Paraná. Subsequently, a race differentiation test was carried out by inoculating differentiating cultivars and observing the pathogen's response, and a sextet code was assigned to each isolate according to its virulence phenotype (EU‐C). Frequency (%), virulence complexity index by isolate and by phenotype, and Gleason index were calculated. A total of 117 isolates were evaluated and 61 virulence phenotypes were identified. The sextet code 31‐00‐00 was the most frequent and could be used for the evaluation of resistant cultivars in the states of the south and southeast regions of Brazil. The resistance factor of lettuce cultivar Balesta provided resistance to all evaluated isolates, while RYZ2164 was resistant to all isolates except those from Paraná. Additionally, Kibrille conferred resistance to isolates from Santa Catarina.

Characterization of a maltononaose-producing amylopullulanase from Bacillus aryabhattai W310.

The recombinated amylopullulanase of PulW310B, pullulanase from Bacillus aryabhattai W310, was characterized. Sequence analysis of PulW310B showed that PulW310B has type I pullulanase structures including its typical region and the conserved regions of glycoside hydrolase family 13. Moreover, PulW310B was predicted to has typical domains of pullulanase and SSF51445 belonging to tansglycosidase. While the substrate specificity of PulW310B presented that it is not a type I pullulanase. PulW310B could hydrolyze pullulan into maltotriose, maltohexaose, and maltononaose as main products with the proportions of 73.6%, 16.7%, and 6.7%, which indicated that PulW310B has a certain potential on maltooligosaccharides production due to its 4-α-transglycosylation activity that was consistent with its domain analysis. Based on substrate specificity and hydrolytic properties, PulW310B is an amylopullulanase. This is the first report of maltononaose producing activity of amylopullulanase, which will be very useful in food industry. Moreover, PulW310B is a moderate pullulanase with optimal temperature of 40°C and pH of 7.0. 50% of the maximum activity was retained at room temperature (25°C), which indicated that it is an environmentally friendly enzyme in maltooligosaccharides production.

Hydrothermal reduction of CO2 into formate in a semicontinuous plant with soft wood as reducing agent

Hydrothermal reduction of CO2 into formate in a semicontinuous plant with soft wood as reducing agent

Evaluation of the effect of sodium alginate, hydroxyethyl cellulose, aspartame, and poly(ethylene oxide)-b-poly(propylene oxide) copolymer on the in-vitro corrosion of AZ31 Mg alloy in simulated body fluid.

Research on Mg-based implants has increased recently because of their compatibility and biodegradability. Despite this promise, challenges related to high corrosion rates hampered wide-scale deployment. This paper explores the inhibiting properties of biomacromolecules, sodium alginate (ALG), hydroxyethyl cellulose (HEC), aspartame (ASP), and poly(ethylene oxide)-b-poly(propylene oxide) copolymer (PEO-b-PPO) on AZ31 Mg alloy in simulated body fluid at 37°C. Results revealed that PEO-b-PPO accelerated, ALG insignificantly inhibited, while ASP and HEC showed moderate inhibition. At 2000ppm, ASP and HEC offered 54% and 53% protection after 48h and over 65% if blended. Mechanistic insights were gained via XPS, FTIR, and distribution of relaxation times (DRT) analysis. Three corrosion mechanisms, Cl- transport, charge transfer, and ion transport across inherent MgO lattice are revealed by DRT and occurred at f=5Hz, f=21Hz and 832Hz, and f=100,000Hz. Inhibitors' presence prolonged the relaxation time or changed the frequency of occurrence. Carbonates (Mg, Ca), hydroxides (Mg), and phosphates are the main corrosion products. Adsorbed ASP and HEC molecules are mixed with these products to protect the alloy. These findings offer a better understanding of the underlying mechanisms that could facilitate the development of target-oriented corrosion inhibitors for Mg.

Key farm characteristics associated with the level of antimicrobial use in rosé veal production - A Danish database study.

Antimicrobial use (AMU) in veal production is high compared to other bovine production types and has been suggested as an area with potential for AMU reduction. High AMU is a public health concern due to its association with antimicrobial resistance (AMR). Identifying farm characteristics associated with AMU could provide valuable insights for stakeholders seeking to monitor and implement initiatives to reduce AMU. This study aimed at investigating farm characteristics associated with AMU in Danish rosé veal farms, which is the main veal production type in Denmark. The AMU and characteristics included were extracted from two Danish national databases; The Danish Central Husbandry Register (CHR) and the Danish Veterinary Medicines Statistics Program (VetStat). Characteristics included were farm size, mortality, number of suppliers, sites per farm, and farm-level composition of animals with regards to sex and breed. The 118 farms included in the study received 41 % of the total amount of antimicrobials prescribed for Danish calves and young stock in 2020 measured in Animal Daily Doses (ADD). A multivariable linear regression model with the annual average farm-level AMU as outcome was created. AMU was measured as ADD per 100 animals per day (ADD100) and square root-transformed in the model. Increasing farm size and number of suppliers and decreasing proportion of crossbred bulls were found to be significantly associated with higher AMU. However, proportion of crossbred bulls was correlated with proportion of females. Separating the effects of breed and sex was not possible, partly due to the highly summarised data structure. Mortality and number of sites were not significantly associated with AMU. Farms with the type "starter-farms" has previously been shown to have a higher AMU compared to other rosé veal farm types. An important finding in this study was that grouping multiple sites into farms by using ownership data made it possible to summarise AMU for the full line of production from arrival at the veal farm to exit for slaughter. The results and approaches from this paper present an opportunity for repeated evaluation of farm characteristics associated with AMU, which could be used to continuously adapt and target AMU monitoring and control. In addition, it is done on existing surveillance data which keeps the cost of the study low in terms of data collection and data management.