Main Processes Research Articles

Joint Analysis of Small RNA and mRNA Sequencing Unveils miRNA-Mediated Regulatory Network in Response to Methyl Jasmonate in Apocynum venetum L.

Apocynum venetum L. is a natural fiber and medicinal plant species with significant economic value. Jasmonic acid is an endogenous growth regulatory substance present in higher plants that participate in plant growth, development, and defense. As important endogenous single-stranded RNA molecules, microRNA (miRNA) plays an important role in the post-transcriptional regulation of plant genes. A combination of miRNA and mRNA sequencing techniques was used to systematically identify the methyl jasmonate miRNAs and mRNAs in A. venetum. Up to 135 conserved and 26 species-specific miRNAs have been identified in A. venetum. These miRNAs mainly target genes that encode transcription factors and enzymes. The expression levels of 23 miRNAs, including miR398 and miR482, significantly changed after MeJA treatment. A total of 1778 genes were differentially expressed under MeJA treatment, of which 825 were upregulated and 953 were downregulated. The main biological processes enriched in these differentially expressed genes were redox balance, secondary metabolism, photosynthesis, and plant hormone signal transduction. Joint analysis of the miRNAs and mRNA revealed that MeJA-responsive miRNAs function by forming regulatory modules, including miR398-CSD, miR482-NBS-LRR, miR156-SPL10, and miR164-NAC056, which further regulate multiple biological processes, including redox balance, disease resistance, and morphogenesis in A. venetum. This study provides important information to understand the biological roles of miRNAs in A. venetum.

Overpressure origin and evolution during burial in the shale gas plays of the Wufeng-Longmaxi formations of southern Sichuan basin

Identifying the origin, evolution and distribution of overpressure poses a significant challenge in shale gas reservoir, particularly in the deeper layers and zones of the Wufeng-Longmaxi Formations. To uncover the origin mechanism and reconstruct the evolution process of overpressure, samples are collected from the Wufeng-Longmaxi Formations in the southern Sichuan Basin. Subsequently, a combination of multi well-logging methods is employed to comprehensively identify the origin mechanism of overpressure. Additionally, forward numerical simulation and Laser Raman inversion validation are utilized to reconstruct the evolution process of overpressure during burial. Results show that: (1) Hydrocarbon generation pressurization is identified as the dominant origin mechanism of overpressure in the Wufeng-Longmaxi shale gas reservoir, employing a combination of well-logging methods, the revised Bowers method, and a velocity-density intersection chart. (2) The Wufeng-Longmaxi shale undergoes three main pressurization processes, including kerogen generation oil pressurization, crude oil cracking gas pressurization and kerogen cracking gas pressurization. Hydrocarbon generation pressurization correlates closely with factors such as organic matter abundance and type, original hydrogen index, kerogen mass fraction and transformation rate, oil cracking rate and retention coefficient. (3) The pressure coefficient of kerogen generation oil pressurization and gas cracking pressurization reaches 1.73 and 2.78, respectively. The contribution of crude oil cracking gas pressurization (75.6 MPa) is significantly greater than kerogen generation oil pressurization (37.8 MPa). (4) The density of methane inclusions (0.290–0.319 g/cm3) is corroborated through both Laser Raman spectroscopy and homogenization temperature analysis. The paleo pressure is recovered as 90.3–193.5 MPa, with a corresponding pressure coefficient spanning from 1.39 to 2.98. This study not only contributes to the advancement of the shale gas accumulation theory, but also provides valuable insights to guide the further exploration and development of marine shale gas within the intricate tectonic setting of the Sichuan Basin.

Using a Combination of High-Frequency Coastal Radar Dataset and Satellite Imagery to Study the Patterns Involved in the Coastal Countercurrent Events in the Gulf of Cadiz

This study exploits the combination of High-Frequency Coastal Radar (HFR) information with satellite-derived observations to characterize the patterns involved in the coastal countercurrents (CCCs) events in the Gulf of Cadiz (GoC), which is situated in the SW of the Iberian Peninsula. The westward alongshore currents are observed throughout the year, but the main drivers necessary to develop this flow and its extension in both parts of the basin are not fully clear. In order to identify the main physical processes (both local and remote) that induce the development of these countercurrents and to evaluate the connection of the circulation patterns between the eastern and the western part of the GoC, we make use of several data sources available for the region. First of all, a land-based system of HFR antennas located at four different sites of the GoC provides the velocity field of the surface circulation of the basin. To achieve a significant characterization of the CCCs in the Gulf, the dataset analyzed is processed by means of a series of operations, including the Empirical Orthogonal Functions (EOFs) analysis used to identify spatial and temporal variability of the flow, a low-pass filter used to isolate the sub-inertial signal of the current and temporal interpolation to fill in the missing values. Secondly, given the known importance of the zonal component of the local winds combined with the variations in the mean pressure at sea level over the Western Mediterranean during these events, time series of meteorological data are processed and correlated with the current velocity series via a statistical analysis. Finally, sea surface temperature fields and chlorophyll-a distribution patterns are used as tracers to obtain information on the extension of the countercurrents where HFR data are missing in four cases studied during the year 2017. The conducted analysis revealed the consistent occurrence of westward coastal currents throughout the year, driven in the most intense cases by a combination of the zonal component of the local wind and atmospheric pressure fluctuations over the Western Mediterranean Sea. During those events, CCCs reached the Portuguese side of the Gulf and facilitated the advection of biological material and warmer waters.

Technology for Isolation Essential Oil from the Buds of Populus balsamifera L.

The article presents the results of the development of technology for isolating essential oil from the buds of Populus balsamifera L. and a medicinal substance based on it. For the first time, a technology has been developed for the quantitative isolation of essential oil from the buds of Populus balsamifera L. by the barothermal method at a temperature of 140 ºC, under a vacuum of 1333‒2000 Pa. The main components of the essential oil of Populus balsamifera L. buds, isolated by the barothermal method, were determined to be α-bisabolol, β-eudesmol, and 2-phenylethyl-2-methylbutanoate. A technological scheme for the production of essential oil substance from Populus balsamifera L. buds has been developed, including 2 stages of the main technological process and 2 stages of auxiliary work, and its pilot industrial regulations. The control points during the isolation and preparative production of the essential oil substance from the Populus balsamifera L. buds are the major components α-bisabolol and β-eudesmol. The main critical points in the production of a substance based on the essential oil of Populus balsamifera L. buds are pressure, temperature, and duration of isolation. The stages of production of the substance are controlled using a standard sample of α-bisabolol (1). A quantitative determination of 108 components of essential oil from Populus balsamifera L. buds was carried out, as well as standardization for organoleptic properties and major components. A comparative analysis of essential oil from Populus balsamifera L. buds collected in the vicinity of Karaganda and the North Kazakhstan region was carried out. A substance based on essential oil from Populus balsamifera L. buds has antimicrobial, wound-healing, and antitumor activities with relatively low toxicity.

Формування комплексного підходу до досліджень впливу транспортно-промислових процесів на сіті-логістичні системи

Taking into account the need for post-war reconstruction, one of the important issues is the formation of city-logistics systems taking into account the prospects for the development of urban industry, advanced achievements of science and technology, and the introduction of ecological modes of transport. , ensuring the European level of social and cultural development of the population. Meanwhile, enterprises of the metallurgical industry, as a rule, are city-forming in the settlements of industrial districts, they employ tens of thousands of workers. Taking into account the continuity of the main production processes in metallurgy, employees of such enterprises have a need for round-the-clock daily movement from their place of residence to the enterprise and back. At the same time, the schedules of the beginning and end of work shifts may be different at the same enterprise. Under such conditions, it is important to provide employees with timely and reliable transportation to the workplace. Accordingly, the city's transport system as a whole and the city's public transport in particular must be ready to ensure the growth of demand for passenger transportation for the sustainable development of the city's economy and social sphere. The article emphasizes the relevance of the tasks of optimizing the duration of signals both for relatively simple configurations of street network nodes and for complex street intersections with several sections of the road separated by a boulevard. The approach proposed by the authors to the management of the city-logistics system in the conditions of the cities of industrial regions will ensure the growth of demand for passenger transportation for the purpose of sustainable development of the economy and social sphere of cities. The need has been proven and practical solutions have been proposed for increasing the level of road safety, reducing traffic jams, and improving traffic modes within the cities of industrial areas, which allows to reduce the number of traffic accidents, ensures the fulfillment of urban logistics tasks, and also reduces the level of energy consumption of vehicles. expedient connection of various types of street transport, including individual ones, which will ensure rational planning of street space and increase the efficiency of the transport system of cities.

ROS/MMP-9 mediated CS degradation in BMSC inhibits citric acid metabolism participating in the dual regulation of bone remodelling

It is necessary to figure out the abnormal energy metabolites at the cellular level of postmenopausal osteoporosis (PMOP) bone microenvironment. In this study, we constructed PMOP model by ovariectomy and identified 9 differential metabolites compared with control femur by energy metabolomic. The enrichment analysis of differential metabolites revealed that tricarboxylic acid cycle, glucagon pathway and purinergic signaling pathway were the main abnormal metabolic processes. Citric acid was identified as the key metabolite by constructing compound reaction–enzyme–gene network. The functional annotation of citric acid targets identified by network pharmacological tools indicated that matrix metalloproteinase 9 (MMP-9) may be involved in regulating citric acid metabolism in the osteogenic differentiation of bone marrow mesenchymal stem cell (BMSC). Molecular docking shows that the interaction forces between MMP-9 and citric acid synthase (CS) is −638, and there are multiple groups of residues used to form hydrogen bonds. Exogenous H2O2 promotes the expression of MMP-9 in BMSC to further degrade CS resulting in a decrease in mitochondrial citric acid synthesis, which leads to the disorder of bone remodeling by two underlying mechanisms ((1) the decreased histone acetylation inhibits the osteogenic differentiation potential of BMSC; (2) the decreased bone mineralization by citric acid deposition). MMP-9-specific inhibitor (MMP-9-IN-1) could significantly improve the amount of CS in BMSC to promote cellular citric acid synthesis, and further enhance bone remodeling. These findings suggest inhibiting the degradation of CS by MMP-9 to promote the net production of citric acid in osteogenic differentiation of BMSC may be a new direction of PMOP research.

The possibility paradox: How technology both expands and contracts possibilities

As we turn more and more to technology to solve the problems it has created – from misinformation to climate change – it is crucial to understand how innovations can both expand the possible and contract it. In this editorial, I first outline how technological advances increase what is possible, probable and feasible, and our sense of it, through four main processes: (1) Expanding what is possible, (2) broadening our sense of what could be, (3) making the possible reachable and (4) freeing up resources to explore new possibilities. I then look at six processes through which technology reduces them and narrows our horizon: (1) by destroying possibilities, (2) forcefully replacing tasks and jobs, (3) leading to a loss of skills, (4) bringing social and environmental destruction, (5) making existing systems obsolete and (6) creating possibility tunnels. This nomenclature is not meant to be exhaustive. It is instead an invitation to fellow researchers interested in possibilities, technologies and their intersection, to explore how novel tools affect our perceptions of what could be and as well as our actual actions.

Interactions between Clays and Carbonates in the Aptian Pre-Salt Reservoirs of Santos Basin, Eastern Brazilian Margin

The giant Pre-salt reservoirs represent most of the oil production in Brazil. The main Aptian sag reservoirs were deposited in a unique and highly complex hyper-alkaline lacustrine setting. These deposits are essentially constituted by fascicular and spherulitic calcite precipitated in a magnesian clay matrix (stevensite, kerolite, and saponite/hectorite). Although vital for understanding the origin and main reservoir quality control, the genesis and interactions of clays and carbonates are still poorly constrained. The detailed petrographic description was focused on 812 thin sections from five wells drilled in the Santos Basin Aptian Barra Velha Formation, combined with cathodoluminescence, UV epifluorescence, and X-ray diffraction analyses. The main syngenetic processes were the deposition of finely laminated peloidal and ooidal Mg-clays, the formation of fascicular calcite crusts on the sediment–water interface, and the redeposition of these materials as intraclasts. Abundant clay peloids engulfed in syngenetic shrubs indicate that calcite and clay precipitation was concomitant, though with highly variable rates. Eodiagenetic phases include matrix-replacive and -displacive spherulites and fascicular shrubs; matrix-replacive blocky calcite and dolomite; lamellar carbonates filling matrix shrinkage pores; and microcrystalline calcite, dolomite, and silica replacing the Mg-clay matrix. The preferential dolomitization and calcitization of peloidal layers were most likely due to their higher permeability and larger specific surface. Matrix-replacive saddle dolomite, macrocrystalline calcite, and dawsonite are interpreted as mesodiagenetic or hydrothermal phases after significant matrix dissolution. Unraveling the processes of the formation and alteration of the carbonates and clays and their interactions in the Pre-salt deposits is essential for constraining the depositional and diagenetic conditions in their unique environments and their diagenetic overprinting and for decreasing the exploration risks and increasing the production of those extraordinary reservoirs.

Digital tools as a key element of human resource management technology in the conditions of digital transformation

Aim. To identify technological features of human resource management (HRM) in the conditions of digital transformation.Objectives. To describe the main directions of HRM technology transformation in modern conditions; to define the concept of "digital tools"; to systematize the digital tools used in modern conditions; to identify promising directions of using digital tools on the way of digital trans- formation.Methods. The authors used in the research process the methods of contextual analysis, modeling terms and models, induction and deduction, synthesis and description, applied methods of comparative, system and structural analysis.Results. The outcome of the digital transformation of business is a progressive process, which in the end will radically affect the functioning of personnel. In this regard, the concept of "digital tools" of HRM is defined, their relationship with HRM technologies is revealed. It is revealed what digital tools are most often used today in Russian companies, whether there is their classification by forms of application and whether digital tools affect the technological process of HRM. The peculiarity of applying HRM tools from the possible set of tools is its scientific substantiation on the one hand and the practice of its application on the other. The basis is the orientation on the solution of a certain problem faced by the company in the current period. In order to get an effective effect, it is necessary to refuse from haphazard ap- plication of tools. A technological process of HRM should be formed, which will be based on a clearly structured sequence of actions that are interchangeable. An important element that should be paid close attention to is the understanding that the new information reality will place increased demands on the competencies of personnel. The article emphasizes that the digital transformation of business should be carried out progressively, taking into account the timely mastering of new digital competencies by employees.Conclusions. The digital transformation of business taking place today launches new, previously unobserved processes that cover all spheres of functioning of real commercial enterprises. Business can get a new quality both in the aspect of formation of management decisions on an objec- tive basis and in the framework of HRM. In this regard, it is safe to say that we are waiting for a systemic restructuring of the main business processes and, as a consequence, the introduction of new HRM tools, on the basis of which it will be possible to design digital HRM technologies.

Investigation of an Optimal Material Addition Rate for Energy Consumption and Dimensional Accuracy in Fused Filament Fabrication of CFR-PEEK.

The material addition rate (MAR) of fused filament fabrication (FFF) is an indicator of process efficiency varied by process parameter settings, which affects energy consumption and part quality in FFF. This study aims to identify the optimal MAR of FFF using carbon-fiber-reinforced polyether-ether-ketone (CFR-PEEK) by considering a trade-off between energy consumption and the dimensional accuracy of FFF outputs. A design of experiments considering two main process parameters is planned to print three sample types through FFF for CFR-PEEK. Then, the MAR (i.e., deposited material volume per build time) of FFF is obtained to derive individual regression models of energy consumption and the dimensional accuracy measured for each sample type. Furthermore, a trade-off between energy consumption and dimensional accuracy on the MAR is formulated to derive an optimal MAR for each sample type. The results show that FFF for CFR-PEEK has a trade-off between energy consumption and dimensional accuracy; there exists a specific MAR that maximizes the overall performance of energy consumption and dimensional accuracy for each sample type. The optimal MAR is the highest for the small volume sample, whereas it becomes the lowest for the vertical build orientation sample. This study suggests that the optimal MAR should be flexibly adjusted based on a fabricated part. The findings from this study also address the fact that decision-making for optimal FFF operations needs a transition from the identification of specific process parameter settings to the management of a proper process efficiency level in FFF.

Research on Radiation Monitoring Strategy for Spent Fuel Reprocessing Plant Based on Coordination of Nuclear Security Risk and Facility Importance Evaluation

This paper examines a method and scheme for optimizing the allocation of nuclear radiation monitoring resources, based on the analysis of the risk of nuclear security events and the importance of facilities in a spent fuel reprocessing plant. By constructing a nuclear security radiation accident tree for a plant, the importance of different security events was calculated using fuzzy mathematics and expert scoring methods. The importance of each facility was determined by establishing a fuzzy comprehensive decision model, and a resource allocation scheme for nuclear radiation monitoring was proposed based on the importance level of facilities. The research findings demonstrated that the extraction process plant in the main process area, and the centralized control room in the front area of the plant, were of highest importance. Accordingly, three levels of nuclear radiation monitoring programs were established based on the importance of each facility. This study offers theoretical and technical support for the safety management and operation of a spent fuel reprocessing plant. Additionally, the analysis results can serve as a reference for allocating nuclear radiation monitoring resources in various facilities in a reasonable manner.

Characterization of the benthic biogeochemical dynamics after flood events in the Rhône River prodelta: a data–model approach

Abstract. At the land–sea interface, the benthic carbon cycle is strongly influenced by the export of terrigenous particulate material across the river–ocean continuum. Episodic flood events delivering massive sedimentary materials can occur, but their short-term impact on carbon cycling is poorly understood. In this paper, we use a coupled data–model approach to estimate the temporal variations in sediment–water fluxes, biogeochemical pathways and their reaction rates during these abrupt phenomena. We studied one episodic depositional event in the vicinity of the Rhône River mouth (NW Mediterranean Sea) during the fall–winter of 2021/22. The distributions of dissolved inorganic carbon (DIC), sulfate (SO42-) and methane (CH4) were measured in sediment porewaters collected every 2 weeks before and after the deposition of a 25 cm sediment layer during the main winter flood event. Significant changes in the distribution of DIC, SO42- and CH4 concentrations were observed in the sediment porewaters. The use of an early diagenetic model (FESDIA) to calculate biogeochemical reaction rates and fluxes revealed that this type of flood event can increase the total organic carbon mineralization rate in the sediment by 75 % a few days after deposition. In this period, sulfate reduction is the main process contributing to the increase in total mineralization relative to non-flood deposition. The model predicts a short-term decrease in the DIC flux out of the sediment from 100 to 55 mmolm-2d-1 after the deposition of the new sediment layer with a longer-term increase by 4 %, therefore implying an initial internal storage of DIC in the newly deposited layer and a slow release over relaxation of the system. Furthermore, examination of the stoichiometric ratios of DIC and SO42- as well as model output over this 5-month window shows a decoupling between the two modes of sulfate reduction following the deposition – organoclastic sulfate reduction (OSR) intensified in the newly deposited layer below the sediment surface, whereas anaerobic oxidation of methane (AOM) intensified at depth below the former buried surface. The bifurcation depth of sulfate reduction pathways, i.e., the sulfate–methane transition zone (SMTZ), is shifted deeper by 25 cm in the sediment column following the flood deposition. Our findings highlight the significance of short-term transient biogeochemical processes at the seafloor and provide new insights into the benthic carbon cycle in the coastal ocean.

A Review of Biological Processes for Dairy Wastewater Treatment and the Effect of Physical Parameters Which Affect Their Efficiency

The global dairy sector produces upwards of 900 million tonnes of milk each year, resulting in 2.25 billion tonnes of wastewater being generated. This is significant in the South African context as a large percentage of the population lives without reliable access to clean, reliable water. The treatment of dairy wastewater (DWW) in this context would help alleviate the unavailability for many people in this country. DWW has a high fat content along with a high chemical oxygen demand (COD), which makes it problematic to dispose of. Biological treatment processes have shown great success in the remediation of this water. However, these are not without their shortfalls. A variety of biological processes have been listed here as well as suggestions to improve their effectiveness. To improve the treatment efficiency, there are two main lines of thinking: First, to optimise the process through the manipulation of the physical parameters of the systems (e.g., temperature, pH, hydraulic retention time (HRT), agitation, etc.). Second, to add either pre-treatment or post-treatment processes to the main process to increase the effectiveness of the entire process overall. These different options have been discussed, with suggestions being made on the way forward.

Cytonuclear interplay in auto- and allopolyploids: amultifaceted perspective from the Festuca-Lolium complex.

Restoring cytonuclear stoichiometry is necessary after whole-genome duplication (WGD) and interspecific/intergeneric hybridization in plants. We investigated this phenomenon in auto- and allopolyploids of the Festuca-Lolium complex providing insights into the mechanisms governing cytonuclear interactions in early polyploid and hybrid generations. Our study examined the main processes potentially involved in restoring the cytonuclear balance after WGD comparing diploids and new and well-established autopolyploids. We uncovered that both the number of chloroplasts and the number of chloroplast genome copies were significantly higher in the newly established autopolyploids and grew further in more established autopolyploids. The increase in the copy number of the chloroplast genome exceeded the rise in the number of chloroplasts and fully compensated for the doubling of the nuclear genome. In addition, changes in nuclear and organelle gene expression were insignificant. Allopolyploid Festuca × Lolium hybrids displayed potential structural conflicts in parental protein variants within the cytonuclear complexes. While biased maternal allele expression has been observed in numerous hybrids, our results suggest that its role in cytonuclear stabilization in the Festuca × Lolium hybrids is limited. This study provides insights into the restoration of the cytonuclear stoichiometry, yet it emphasizes the need for future research to explore post-transcriptional regulation and its impact on cytonuclear gene expression stoichiometry. Our findings may enhance the understanding of polyploid plant evolution, with broader implications for the study of cytonuclear interactions in diverse biological contexts.

Study on inverse geochemical modeling of hydrochemical characteristics and genesis of groundwater system in coal mine area - a case study of Longwanggou Coal Mine in Ordos Basin.

The exploitation of coal resources has disturbed the equilibrium of the original groundwater system, resulting in a perturbation of the deep groundwater dynamic conditions and hydrochemical properties. Exploring the formation of mine water chemistry under the conditions of deep coal seam mining in the Ordos Basin provides a theoretical basis for the identification of sources of mine water intrusion and the development and utilization of water resources. This paper takes Longwanggou Coal Mine as the research area, collects a total of 106 groups of water samples from the main water-filled aquifers, comprehensively uses Piper trilinear diagram, Gibbs diagram, ion correlation, ion ratio coefficient and mineral saturation index analysis, and carries out inverse geochemical modeling with PHREEQC software, so as to analyze the hydrochemical characteristics and causes of the main water-filled aquifers in deep-buried coal seams in the research area. The results show that the main hydrochemical processes in the study area are leaching and cation exchange, and the groundwater is affected by carbonate (calcite, dolomite), silicate (gypsum) and evaporite. Calculations of mineral saturation indices and PHREEQC simulations have led to the conclusion that the dissolution of rock salt and gypsum in groundwater accounts for most of the ionic action. Na+, Cl- and SO42- are mainly derived from the dissolution of rock salt and gypsum minerals, while Ca2+ and Mg2+ are mostly derived from the dissolution of dolomite and calcite. The results of the inverse geochemical modeling are consistent with the theoretical analysis.

Isotopomeric Peak Assignment for N2O in Cross-Labeling Experiments by Fiber-Enhanced Raman Multigas Spectroscopy.

Human intervention in nature, especially fertilization, greatly increased the amount of N2O emission. While nitrogen fertilizer is used to improve nitrogen availability and thus plant growth, one negative side effect is the increased emission of N2O. Successful regulation and optimization strategies require detailed knowledge of the processes producing N2O in soil. Nitrification and denitrification, the main processes responsible for N2O emissions, can be differentiated using isotopic analysis of N2O. The interplay between these processes is complex, and studies to unravel the different contributions require isotopic cross-labeling and analytical techniques that enable tracking of the labeled compounds. Fiber-enhanced Raman spectroscopy (FERS) was exploited for sensitive quantification of N2O isotopomers alongside N2, O2, and CO2 in multigas compositions and in cross-labeling experiments. FERS enabled the selective and sensitive detection of specific molecular vibrations that could be assigned to various isotopomer peaks. The isotopomers 14N15N16O (2177 cm-1) and 15N14N16O (2202 cm-1) could be clearly distinguished, allowing site-specific measurements. Also, isotopomers containing different oxygen isotopes, such as 14N14N17O, 14N14N18O, 15N15N16O, and 15N14N18O could be identified. A cross-labeling showed the capability of FERS to disentangle the contributions of nitrification and denitrification to the total N2O fluxes while quantifying the total sample headspace composition. Overall, the presented results indicate the potential of FERS for isotopic studies of N2O, which could provide a deeper understanding of the different pathways of the nitrogen cycle.

The Century of the Crises, the Century of the Accumulation: Two Sides of the Same Coin

ABSTRACT The hundred-year history of capitalism in Turkey can be discussed in different contexts. This study addresses this process by focusing on economic crises. It argues that the history of capitalism in Turkey can be interpreted as a transition from one crisis to another. This paper suggests reading the crises in the context of the two main accumulation processes: the commercial capital accumulation process and crises (1929 and 1954-8) and the crises of industrial capital accumulation (1978/79, 1989, 1994, 2001, 2008, 2018/2019). The 1978/79 crisis is considered as a crisis of inward-oriented capital accumulation, 1989 as a crisis of export-oriented capital accumulation, and 1994, 2001, 2008, and 2018/2019 as the crises of capital import-oriented accumulation. This study reads these crises that have occurred in different periods as a result of the foreign exchange production and acquisition problem stemming from the peculiarities of late capitalist development.

Spatial distribution characteristics and interaction effects of DOM and microbial communities in kelp cultivation areas

The influence of macroalgae cultivation on aquaculture carbon sinks is significant, with microbial carbon (C) pumps contributing to a stable inert dissolved carbon pool in this context. Concurrently, dissolved organic matter (DOM) exchange at the marine sediment-water interface profoundly affects global ecosystem element cycling. However, the interactions between DOM and bacterial communities at the sediment-water interface in kelp cultivation areas, especially regarding microbial function prediction, have not been fully explored. This study analyzed the DOM characteristics, environmental factors, and bacterial community structure in the Tahewan kelp——Saccharina japonica cultivated area and compared them with those in non-cultivated areas. The results indicated significantly higher dissolved organic carbon (DOC) concentrations in the kelp culture area, particularly in surface seawater and overlying water. The dominant bacterial phyla in both regions included Pseudomonadota, Actinomycetota, and Bacteroidota in both regions, while Desulfobacterota was more prevalent in the sediment environment of the cultivated region. Parallel factor analysis (EEM-PARAFAC) was used to identify DOM components, among which component C2 (a microbial humic-like substance DOM) was highly resistant to microbial degradation. We infer that C2 has similar properties to recalcitrant dissolved organic matter (RDOM). Analysis of the predicted functional genes based on 16S rRNA gene data showed that methanol oxidation, methylotrophy, and methanotrophy were significant in the bottom seawater of the cultivation area. The carbon (C), nitrogen (N), and sulfur (S) cycle functional genes in the sediment environment of the kelp cultivation area were more active than those in other areas, especially in which sulfate reduction and denitrification were the two main processes. Furthermore, a DOM priming effect was identified in the cultivated sediment environment, where kelp-released labile dissolved organic matter (LDOM) stimulates rapid degradation of the original RDOM, potentially enhancing C sequestration.

Microglial inhibition alleviates alpha-synuclein propagation and neurodegeneration in Parkinson’s disease mouse model

The accumulation of alpha-synuclein (αSyn) is widely recognized as the main pathological process in Parkinson’s disease (PD). Additionally, neuroinflammation is considered to be one of the contributing mechanisms in the development of PD. In light of this, it is hypothesized that the reactive microglia exacerbate the propagation of αSyn and neurodegeneration, while the inhibition of microglial activity may mitigate these effects. To test this hypothesis, αSyn preformed fibrils (PFF)-injected PD mouse model was employed. Co-injection of lipopolysaccharide (LPS) and PFF was performed to investigate if microglial reactivity intensified αSyn propagation and neurodegeneration. Additionally, oral administration of PLX5622, a microglial inhibitor that targets the colony-stimulating factor 1 receptor, was given for two weeks before and after PFF injection each to explore if microglial inhibition could prevent or reduce αSyn pathology. Intrastriatal co-injection of LPS and PFF resulted in increased microglial reactivity, αSyn accumulation, and neurodegeneration compared to PFF injection alone. However, treatment with PLX5622 significantly suppressed microglial reactivity, reduced αSyn pathology, and alleviated dopaminergic neuron degeneration in the PD mouse model injected with PFF. Based on these findings, it is evident that microglial reactivity plays a crucial role in the progression of αSyn pathology and neurodegeneration in PD. Furthermore, the results suggest that microglial inhibition may hold promise as a therapeutic strategy to delay the progression of αSyn pathology in PD.

Numerical parameter sensitivity analysis of residual stresses induced by deep rolling for a 34CrNiMo6 steel railway axle

To optimise the benefits of the deep-rolling process in the service life context of treated components, the process application must be investigated. In addition to the reduction in surface roughness and near-surface material strengthening, compressive residual stresses are introduced, which are primarily responsible for the increase in service life for components, especially in the case of high-strength steel materials. A numerical parameter sensitivity analysis is performed in order to investigate the introduced residual stresses in detail. For this purpose, a validated deep-rolling simulation model is used, which replicates the deep rolling of a railway axle made of the high-strength steel material 34CrNiMo6. The model is based on an elastic-plastic Chaboche material model parameterised on uniaxial tensile and LCF test results and validated with residual stress measurements. Using this model as a basis, the effect of the main process parameters deep-rolling force, feed rate, friction coefficient, number of overruns, tool geometry, and shaft geometry on the resulting residual stress state are investigated. The results reveal that the deep-rolling force has the most significant influence on the introduced residual stress state and should therefore be highlighted. In the case of applying a deep-rolling force of more than 10 kN, maximum compressive residual stresses of around − 1000 MPa are introduced, and a strong saturating behaviour is shown. Maximum compensating tensile residual stresses of + 100 MPa occur below the surface. The main influence of the deep-rolling force is the effective depth achieved, which is determined by the depth of the zero crossing. This varies from 1 mm with an applied force of 2 kN to more than 3.5 mm with 20 kN. Furthermore, the results are analysed to conclude suggestions for the process’s applicability, and a proposal for an optimised deep-rolling treatment is presented. There multiple deep rolling with decreased deep-rolling forces is used to achieve a comparably optimised residual stress state. In summary, with the presented results, a contribution to a deeper understanding of the deep-rolling process can be achieved; the influence of the most important process parameters on the residual stress in-depth profiles is established; an optimisation proposal is presented; and correlations are found. Thus, the base work for further fatigue strength assessments and the optimisation of the deep-rolling process regarding the increase of service is laid.