Main Hematological Parameters Research Articles

Inflammation and blood cells in cardiovascular diseases

Coronary artery disease (CAD) is still the most important cause of death in developed societies. Atherosclerosis is recognized as a systemic immune inflammatory disease. Inflammation in CAD can be both local and systemic. The main advantage of hematological parameters and indices is that they are relatively inexpensive and therefore common and easy to find in daily clinical practice. In this review, we will try to explain the main hematological parameters and their effects on pathophysiology in patients with atherosclerotic cardiovascular disease.

Аnalysis of the influence of basic acrylic plastics "Ftoraks" and "Villacryl H Plus" on the main hematological parameters in the experiment

Summary. The effectiveness of orthopedic treatment significantly depends on the properties of the basic plastics used in the manufacture of dentures.
 The aim of the study – to study the effect of acrylic plastics "Ftoraks" and "Villacryl H Plus", the polymerization of which was carried out by different methods, on the main hematological parameters.
 Materials and Methods. The study and comparative characterization of the possible toxic effects of dental base acrylic plastics "Ftoraks" and "Villacryl H Plus", which were polymerized by different methods, on the main hematological parameters were performed on white outbred rats, which implanted samples of these materials in periodontal tissue.
 Results and Discussion. As a result of the study, it was found that animals implanted with samples of the above acrylic plastics polymerized in a "water bath" methodic in the blood had a decrease of hemoglobin, leukocytosis, increased number of band and segment nuclear neutrophils, increase in the number of eosinophils and decrease of basophils in comparison with the control and other groups of animals. In the study of samples polymerized in the apparatus for dry polymerization under pressure and in the apparatus for molding plastics, it was found that fluctuations in the content of the main hematological parameters are within the physiological norm and do not differ significantly from the control.
 Сonclusions. Dental basic acrylic plastics "Ftoraks" and "Villacryl H Plus" polymerization of which was carried out by the method of dry polymerization with pressure and in the apparatus for foundry pressing of plastics cause less toxic effects on hematological parameters of the blood.
 
 

Microcirculatory and Rheological Adaptive Mechanisms at High Altitude in European Lowlander Hikers and Nepalese Highlanders.

Physical activity at high-altitudes is increasingly widespread, both for tourist trekking and for the growing tendency to carry out sports and training activities at high-altitudes. Acute exposure to this hypobaric-hypoxic condition induces several complex adaptive mechanisms involving the cardiovascular, respiratory and endocrine systems. A lack of these adaptive mechanisms in microcirculation may cause the onset of symptoms of acute mountain sickness, a frequent disturbance after acute exposure at high altitudes. The aim of our study was to evaluate the microcirculatory adaptive mechanisms at different altitudes, from 1350 to 5050 m a.s.l., during a scientific expedition in the Himalayas. The main haematological parameters, blood viscosity and erythrocyte deformability were assessed at different altitudes on eight European lowlanders and on a group of eleven Nepalese highlanders. The microcirculation network was evaluated in vivo by conjunctival and periungual biomicroscopy. Europeans showed a progressive and significant reduction of blood filterability and an increase of whole blood viscosity which correlate with the increase of altitude (p < 0.02). In the Nepalese highlanders, haemorheological changes were already present at their residence altitude, 3400 m a.s.l. (p < 0.001 vs. Europeans). With the increase in altitude, a massive interstitial oedema appeared in all participants, associated with erythrocyte aggregation phenomena and slowing of the flow rate in the microcirculation. High altitude causes important and significant microcirculatory adaptations. These changes in microcirculation induced by hypobaric-hypoxic conditions should be considered when planning training and physical activity at altitude.

Antimitotic effects of &lt;i&gt;Cysticercus tenuicollis&lt;/i&gt; protoscolexes extract at administration to mice and their negative consequences for organism

The purpose of the research is studying of Cysticercus tenuicollis protoscolexes extract effects on cell division at different routes of administration to mice and evaluation of the associated negative effects.Materials and methods. C. tenuicollis were obtained from spontaneously infected sheep in Kabardino-Balkarian Republic. C. tenuicollis protoscolexes were washed, crushed and homogenized. Protein extraction was performed with phosphate buffered saline pH 7.2–7.4. C. tenuicollis extract was administered intraperitoneally and intravenously to mice males at the dose level of 80 μg protein/animal. The control group of mice was intravenously injected with 0.1 ml of saline. At hours 3; 6; 24 and 48 post extract administration mice were euthanized. Bone marrow samples were taken from experimental and control mice for preparation of microscopic preparations to assess mitotic activity in a given cell population. The mitotic index was determined, all stages of mitosis were recorded. At the above time points blood samples were taken from mice to determine the main hematological parameters post intravenous and intraperitoneal administration of C. tenuicollis extract. The main hematological parameters of mice were determined using hematological analyzer MicroCC-20 Plus (High Technology, Inc. (USA)); leukocyte formula – by the generally accepted method. Samples of liver, kidneys, spleen, mesenteric lymph nodes and testes were taken from experimental and control animals for macroscopic and microscopic studies.Results and discussion. C. tenuicollis protoscolices extract leads to inhibition of cell division in the population bone marrow and testes cells in mice when administered intravenously and intraperitoneally at the dose level of 80 μg/animal manifested in accumulation of metaphases and decrease of other stages. At both routes of administration a decrease in leukocyte counts was noted. The observed microscopic changes in testes, spleen and lymph nodes either reflect the consequences of extract antimitotic effect or the immune response to the administration of C. tenuicollis extract.

Safety and Pharmacokinetics of the Substance of the Anti-Smallpox Drug NIOCH-14 after Oral Administration to Laboratory Animals.

Since most of the modern human population has no anti-smallpox immunity, it is extremely important to develop and implement effective drugs for the treatment of smallpox and other orthopoxvirus infections. The objective of this study is to determine the main characteristics of the chemical substance NIOCH-14 and its safety and bioavailability in the body of laboratory animals. The safety of NIOCH-14 upon single- or multiple-dose intragastric administration was assessed according to its effect on the main hematological and pathomorphological parameters of laboratory mice and rats. In order to evaluate the pharmacokinetic parameters of NIOCH-14 administered orally, a concentration of ST-246, the active metabolite of NIOCH-14, in mouse blood and organs was determined by tandem mass spectrometry and liquid chromatography. The intragastric administration of NIOCH-14 at a dose of 5 g/kg body weight caused neither death nor signs of intoxication in mice. The intragastric administration of NIOCH-14 to mice and rats at doses of 50 and 150 µg/g body weight either as a single dose or once daily during 30 days did not cause animal death or critical changes in hematological parameters and the microstructure of internal organs. The tissue availability of NIOCH-14 administered orally to the mice at a dose of 50 µg/g body weight, which was calculated according to concentrations of its active metabolite ST-246 for the lungs, liver, kidney, brain, and spleen, was 100, 69.6, 63.3, 26.8 and 20.3%, respectively. The absolute bioavailability of the NIOCH-14 administered orally to mice at a dose of 50 µg/g body weight was 22.8%. Along with the previously determined efficacy against orthopoxviruses, including the smallpox virus, the substance NIOCH-14 was shown to be safe and bioavailable in laboratory animal experiments.

A QUINOSOL-BASED DRUG FOR THE PREVENTION AND TREATMENT OF POSTPARTUM COMPLICATIONS IN COWS

The paper presents the study results of a drug designed to treat and prevent postpartum uterine inflammatory diseases in cows based on quinazoline - Hinasept-gel. The authors used the results of a toxicological study, the study of antimicrobial activity, its study of hematological parameters, natural resistance, and the identification of the prophylactic and therapeutic efficacy of Hinasept-gel. The authors also found that the drug has a complex therapeutic effect, showing a high preventive (85.2%) and therapeutic (96.4%) effect, accompanied by improved livestock reproduction. In addition, the drug does not have a toxic effect on animals in therapeutic doses, does not have an allergenic and sensitizing impact, and does not irritate the uterine mucosa. Also, the medicine normalizes the main hematological and immunological parameters within 15 days of its use.

ВЛИЯНИЕ ЖИДКОЙ ВИТАМИННОЙ ДОБАВКИ НА ПРИРОСТ ЖИВОЙ МАССЫ И ГЕМАТОЛОГИЧЕСКИЕ ПОКАЗАТЕЛИ КРОЛИКОВ

The article presents the results of research aimed at studying the effect of the Nitamin liquid vitamin supplement on changes in live weight and the main hematological parameters of young rabbits. The studies were carried out at the Department of Parasitology and Veterinary and Sanitary Expertise of the Federal State Budgetary Educational Institution of Higher Education MGAVMiB – MVA named after K.I. Scriabin in the period 2021–2022. The objects of the study were young rabbits bred by crossing two meat breeds, the Soviet chinchilla and the New Zealand white. The drug «Nitamin» is a liquid vitamin drink supplement aimed at replenishing the deficiency of vitamins A, D3 , E and C in the body of animals. «Nitamin» experimental animals received with water for drinking four days from the age of 60 days and from the age of 74 days at the rate of 1 ml/1 liter of water. The results of the studies indicate that the rabbits who received the drug "Nitamin" with water for drinking, gained live weight more intensively, the weight of the experimental rabbits at the end of the experiment was 3.77 ± 0.26 kg, which is 12.87% more than in control group. The main hematological parameters of young rabbits after the use of the medicinal drinking supplement "Nitamin" were within the physiological norm without pronounced deviations. The amount of hemoglobin in the blood of rabbits of the experimental group at the end of the experiment significantly increased by 28.74% and amounted to 136.14±4.26 g/l. The content of total protein in the blood serum of rabbits of the experimental group significantly increased by 4.81% and amounted to 59.23±3.13 g/l. The obtained research results indicate that the drug "Nitamin" can be used as a safe supplement for the main diet of rabbits in order to increase live weight without exposing their body to any dangerous and harmful effects.

THE PRODUCTIVITY FORMATION AND FEATURES OF YOUNG RABBITS BLOOD PARAMETERS UNDER THE INFLUENCE OF A PHYTOBIOTIC

Biologically active feed additives of plant origin (phytobiotics) contribute to the correction of the digestion process, affect the immunity of rabbits, as a result of optimizing the protective functions of the body, and ensure the greatest manifestation of genetic potential by animals. In the context of the above, the implementation of programs for the qualitative improvement of domestic rabbit breeds according to the determinants that form the high biological and nutritional value of products depends on the level of influence of many genotypic and paratypic factors that change significantly over time. One of the most important factors is feeding conditions. It was established that the simultaneous use of a phytosupplement made of dioecious nettle by the kindle of the III group via organism mother's and orally from the 21st day after birth made it possible to increase their live weight in all periods of growth, and on the 90th day this difference reached 14,3 and 7.7 % against peers of the II and I groups. At the age of 120 days, according to the live weight index, they probably exceeded the animals of the II group by 196.5 g or 8,5 % (р&lt;0.001) and the I group – by 166.5 g or 7.1 % (р&lt;0.001). Feeding control is carried basing on the blood parameters, which, in combination with other parameters of the body, make it possible to detect imperceptible changes in organs and tissues, as well as to have an idea of the functional state of both individual organs and the general condition of the animal. Nevertheless, the obtained information provides controllability of the processes that affect the productivity of animals. As part of the research, it was found that the main hematological parameters of the blood of young rabbits of all groups were within the physiological norm during the experiment.On the other hand, at the beginning of the experiment, peripheral blood samples of group III young animals were characterized by a slightly higher content of erythrocytes by 3.6 and 8.0%, hemoglobin by 1.5 and 6.3%, and total protein concentration by 1.8 and 1.5% , albumins – by 1.9 and 3.4% and globulins – by 1.1 and 2.6 %. Feeding the young with a phytosupplement during the natal and post-embryonic period led to an increase in the level of almost all blood components in the blood, but their values did not exceed 9.0% (0.9–8.6%), with no probable difference between the groups, except for the content of erythrocytes, where the difference between animals of III and II groups was 10.5%, with a statistically significant difference between them at the level of p&lt;0.05. The results of the conducted research reveal new opportunities for realizing the genetic potential of the young rabbits productivity by including into their diet the phytosupplement of plant origin – dioecious nettle. Keywords: young, dioecious nettle, live weight, blood parameters, average daily increase.

Evolution of some Haematological Parameters during the Process of Spontaneous Moult in Hy-Line Brown Hens

The purpose of this paper was to find out the evolution of the main haematological parameters in Hy-Line var. Brown hens raised in industrial system, during a natural moult process. The process of spontaneous moult started towards the end of the laying cycle (63 weeks of age) and lasted about seven weeks. Two groups were set up for monitoring: a control group of hens that did not show signs of moult and an experimental group of spontaneously moulted hens. Blood was sampled weekly, from the moment of observing the onset of moult, to the complete re-dressing with feathers, respectively a period of seven weeks. Haematological parameters showed a specific dynamic during this process. This dynamic is dominated by a decrease in red blood cells, packed cell volume and haemoglobin (P&lt;0.05), on the one hand, and a decrease in the white cell number and in the percentage of lymphocytes (P&lt;0.05) on the other. These changes take place predominantly in the first three weeks of the moult period. In the next period of the process, the modified haematological parameters return to the values comparable to those of the control (P &gt; 0.05).

Hematological Parameters from the Feline Blood Donor to the Blood Unit: What Changes Are to Be Expected?

Simple SummaryFeline blood donation requires sedation to allow for good blood collection, avoiding venous damage and unnecessary donor stress. In the present study, we describe the variation of hematological parameters in a population of healthy blood-donor cats that underwent the same sedation protocol, including medetomidine, alfaxalone, and butorphanol. Significant differences in hematological parameters were observed between unsedated and sedated cats; particularly, the mean red blood cell count, hemoglobin concentration, hematocrit value, mean corpuscular volume, and red blood cells distribution width were significantly lower in sedated cats than in conscious ones, thus confirming that sedation is a critical procedure in cats. No significant differences for the main hematological parameters were observed between sedated cats and feline blood units, suggesting that the dilution with the conservative–anticoagulant solution (ratio 1:7) exerts negligible effects on these parameters with respect to samples of sedated animals.Feline blood donation requires sedation to allow for good blood collection, avoiding venous damage and unnecessary donor stress. In the present study, we describe the variation of hematological parameters in a population of 74 healthy blood-donor cats that underwent the same sedation protocol, including medetomidine, alfaxalone, and butorphanol. Changes in hematological parameters were evaluated in blood samples collected from conscious cats (group A), sedated cats (group B), and feline whole-blood units (FBUs) (group C). Significant differences were observed between unsedated and sedated cats: the mean RBC count, HGB, HCT, and RDW were significantly lower in sedated cats than in conscious ones, with a difference of 17.95%, 18.42%, 28.21%, and 10.00%, respectively. In accordance with previously reported data, our results confirm that sedation is a critical procedure in cats that can affect most of the hematological parameters. The second finding of our study is that no significant differences for the main hematological parameters were observed between sedated cats and FBUs, thus suggesting that the dilution with the conservative–anticoagulant solution exert negligible effects on these parameters with respect to samples of sedated animals. This hematological change must be taken into consideration since such parameters are important to define the quality of FBUs.

Comparison of Female Verzaschese and Camosciata delle Alpi Goats' Hematological Parameters in The Context of Adaptation to Local Environmental Conditions in Semi-Extensive Systems in Italy.

Simple SummaryLivestock biodiversity is being lost at an alarming rate. It is mainly due to genetic selection for desirable traits and the standardization of productions, but this has determined a dramatic reduction in intra- and inter-breed genetic variability. Autochthonous breeds represent a pivotal genetic resource thanks to their environment-specific adaptive features, especially related to rusticity, frugality, fertility, and longevity. In this context, it is of fundamental importance to deepen the knowledge about these breeds’ physiology and to take care of their health in the optic of conservation strategies. This study established hematological reference values in female goats of a cosmopolitan (i.e., Camosciata delle Alpi) and an Italian autochthonous (i.e., Verzaschese) breed reared in the same conditions. The influence of breed, age, and season on the hematological parameters was also investigated. The results have shown that variation due to breed, age, and season in blood parameters could be attributed both to physiological changes, such as the ones given by ageing and melatonin effect, and to adaptive genetic processes, for example, towards gastrointestinal parasitism. These findings can be helpful to provide evidence of the importance of recovering endangered/rare local breeds, which are a fundamental heritage for agricultural biodiversity, and local culture.Local livestock breeds are pivotal to ensure sustainable agriculture and represent a real genetic resource in the context of genetic variability reduction. This study aimed at broadening the knowledge about hematological values in female Verzaschese and Camosciata delle Alpi goats (an Italian local and a cosmopolitan goat breed, respectively) and investigating factors affecting them (i.e., breed, age, season). Blood samples were collected monthly from 34 Verzaschese and 37 Camosciata delle Alpi female goats kept under the same semi-extensive farming system for a whole year. The main hematological parameters were evaluated, and descriptive as well as inferential statistical analyses were performed. Reference intervals for complete blood cell count were established and several inter-breed differences were found. In particular, most of the red blood cell parameters were higher in Verzaschese than Camosciata delle Alpi (p < 0.05) suggesting a greater gastrointestinal parasites’ resilience of the local breed. The age effect (p < 0.05) was consistent with the literature while the season effect (p < 0.05) could be explained by the melatonin immunostimulant action and gastrointestinal parasitism influence. Overall, differences in blood values could be attributed to physiological changes and adaptive strategies developed through centuries highlighting the remarkable rusticity and adaptation to the environment and farming system of the local breed.

Increased platelet thrombus formation under flow conditions in whole blood from polycythaemia vera patients.

Polycythaemia vera is a myeloproliferative neoplasm characterised by a high incidence of thrombosis. The contribution of platelets, key players in haemostasis, in this setting is still unclear. So far, the majority of studies have been focussed on specific platelet abnormalities but not on their actual capacity to form thrombi. The aim of this study was to characterise, ex vivo under flow conditions, the capacity of platelets from patients with polycythaemia vera to adhere to collagen and induce thrombus formation. Thirty-nine patients and 30 healthy controls were studied. Thrombus formation was induced by perfusing whole blood over a collagen-coated surface, in a parallel-plate flow chamber coupled to a fluorescent microscope. This dynamic system enables platelet adhesion and thrombus formation to be followed in real time and also allows measurements of the extent of the thrombus and platelet surface antigen expression. Laboratory data were analysed in the light of the patients' main haematological parameters and therapies. Platelet adhesion was significantly greater in patients than in control subjects. Patient thrombi were usually larger and more complex than those formed by control platelets. A significant positive correlation was found between platelet adhesion and both the haematocrit and red blood cell count. These parameters remained significantly correlated with platelet adhesion also after multivariable analysis adjusted for gender, age, therapy and JAK2V617F allele burden. Furthermore, subjects with a haematocrit >45% had significantly greater platelet adhesion than subjects with a haematocrit <45%. Our data indicate that increased platelet adhesion participates in the thrombotic diathesis of patients with polycythaemia vera, and that the haematocrit level can affect the adhesive and thrombus forming capacities of platelets.

Effect of Lanthanum Zirconate Ceramic on the Dynamics of Hematological Parameters and the Bone Remodeling Markers: Experimental Study

Background. Zirconium oxide is actively used in medicine; however, research is underway to improve mechanical characteristics and biointegration. One of the promising areas is the study of materials based on lanthanum zirconate (LZ).&#x0D; The study aimed to examine the effect of a new ceramic material based on LZ on the dynamics of hematological parameters and markers of bone tissue remodeling after intramedullary osteosynthesis (IO) of a hip fracture with LZ rods.&#x0D; Material and Methods. The ceramic material La1.95Ca0.05Zr2O7 was used. The experiment was conducted in guinea pigs, which were divided into four groups: main group, modeling of a hip fracture (IO of the fracture with LZ rod, n = 9); comparison group, modeling of a hip fracture (IO of a fracture with a rod from b-tricalcium phosphate [TCP]; n = 9); control (C) group, modeling of a hip fracture without IO (n= 9); and native control (NC) group. Animals were withdrawn from the experiment before surgery (NC) and at 4, 10, and 25 weeks after surgery (n=3 for each time point). Hematological parameters, i.e., a tartrate-resistant acid phosphatase (TRAP) as an osteoresorption marker and osteocalcin (OC) as an osteogenesis marker, were determined.&#x0D; Results. The red blood cell counts in all groups of the operated animals at 4, 10, and 25 weeks after surgery were not significantly different from the NK group. A significantly higher level of leukocytes in comparison with other groups was observed in the control group 10 weeks after surgery (p = 0.044), which was explained by the absence of fracture synthesis. The platelet level in all groups of the operated animals during the study period was not significantly different from the NK group. The TRAP activity in the LZ and TCP groups had maximum values after 4 weeks, and the OC level reached the maximum by 10 weeks after the operation without significant differences between the LZ and TCP groups of animals.&#x0D; Conclusion. The study of the main hematological parameters did not reveal a negative effect of LZ on the experimental animals. A positive effect of this material on bone tissue remodeling was found. A new ceramic material based on LC appears to be promising for use in traumatology and orthopedics.

Study of Hematological Characteristics and Mineral Metabolism of Moscow Black Chickens in the Process of Acclimatization in the Astrakhan Region

The study of indicators characterizing the processes associated with the acclimatization of birds in new biogeochemical conditions is of interest in connection with the prospects of the poultry industry in the Astrakhan Region, where the lack of important trace elements in soils and plant feeds: Se, I, Co, is observed. In the presented study, the main hematological and biochemical parameters of blood and mineral metabolism were determined in 2 groups of chickens of the Moscow black breed: imported to the Astrakhan Region from Moscow and kept in the Moscow Region. The analyses revealed strong differences (P&lt;0.01) in indicators of two groups. The acclimatized birds were characterized by low levels of red blood cells and hemoglobin in the blood, as well as a higher content of white blood cells than in the second group. The chickens had impaired lipid and protein metabolism. A 71.3% increase in the level of total sugar most likely indicated a stress state of birds. Compared with the chickens in the Moscow Region, the level of calcium in their blood was 32.4% lower, phosphorus – 12.9%. To a large extent, a decrease in the levels of iodine and selenium – by 5.3 and 5.2 times, respectively, was observed. Thus, in the absence of external changes, acclimatized chickens are subject to a latent form of combined I- and Se-hypomicroelementosis. At the same time, a drop in egg productivity by 19% is the recorded fact. The necessity to adapt to the new climatic conditions of the Lower Volga region for birds, combined with a lack of important trace elements, can cause increased oxidative stress in acclimatized birds. Based on the results of the study, the use of feed additives that make up for the deficiency of necessary trace elements is recommended.

Effects of Eucalyptus globulus leaf extract on growth performance, feed utilization and blood biochemistry of Nile tilapia, Oreochromis niloticus

The effects of Eucalyptus globulus Labill. extract in diets of Nile tilapia (Oreochromis niloticus Linnaeus, 1758) were studied. Five diets (500 g each) supplemented with 0 ml (T 0), 5ml (T 5), 10 ml (T 10), 15 ml (T 15) and 20 ml (T 20) of E. globulus extract were fed to O. niloticus for 12 weeks. Group fed diet T 10 recorded the highest final weight (42.27 g), weight gain (36.97 g) and specific growth rate (3.72). The highest protein efficiency ratio (2.36), feed efficiency (0.70) and condition factor (1.31) were observed in group fed diet T 10. Compared to fish fed diet T 0, all groups fed E. globulus extract recorded significantly higher levels of crude protein content in the whole body. Hemoglobin concentration increased with increasing levels of E. globulus extract up to T 10 but decreased after further increase in E. globulus extract in diets. Increasing E. globulus extract levels in diets resulted in a significant decrease in aspartate aminotransferase. Group fed diet T 5 recorded the least (17.50) alkaline phosphate and was significantly lower than all other groups. Globulin concentration decreased with increasing E. globulus extract in diets. This study concludes that dietary administration of E. globulus promotes growth performance, feed utilization and increases the main hematological and biochemical parameters of O. niloticus fish. For optimum growth, 10 ml per 500 g (T 10) feed is recommended. This study provides new information on applications of eucalyptus and, at the same time, promotes rational development and utilization of eucalyptus resources.

Determination of the Photoditazine photosensitizer solution effect on the productivity and microflora of broiler chickens

The scientific work analyzes the effectiveness of the Photoditazine photosensitizer for rearing broiler chickens to prevent infectious diseases, in particular salmonellosis. In the conducted studies, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed by the example of a second-generation photosensitizer - Photoditazine, the active substance of which is dimeglumine chlorin E6. Comparisons of weight gain in broiler chickens from experimental groups were carried out, the composition of the microflora of experimental groups was determined. The harmlessness of the oral use of the Photoditazine photosensitizer was revealed by measuring the main hematological and biochemical parameters of experimental bird groups. Based on the results obtained, it can be concluded about the effectiveness of the use of Photoditazine photosensitizer to maintain the well-being of production poultry livestock.

ЕСТЕСТВЕННАЯ РЕЗИСТЕНТНОСТЬ ПОРОСЯТ ПРИ СКАРМЛИВАНИИ МИКРОБНОЙ ДОБАВКИ

The influence of a microbial supplement upon the introduction of crossbred boletus F2 (CBxL)xD) into the diet during the growing period on nonspecific immunity was studied. It has been established that the inclusion of a microbial supplement in the diet had a positive effect on the main indicators of nonspecific immunity of the pig organism. The evaluation revealed significantly maximum values of bactericidal and lysozyme activity in the second experimental group of 46.93% and 42.81%, respectively. Analysis of the main hematological parameters in the blood of piglets shows that hemoglobin in the 2nd group was higher by 12.67% (P˃0.05) in relation to the control, hematocrit by 7.57% (P&gt;0.05), the amount leukocytes ‒ by 25.03% (P&gt;0.05), and in 1 experimental group ‒ by 17.2% compared to the control group.

Setting for "Normal" Serum Ferritin Levels in Patients with Transfusion-Dependent Thalassemia: Our Current Strategy.

This cross-sectional study aimed to establish the association between serum ferritin levels and organ iron overload (IO) and overall morbidity in transfusion-dependent thalassemia (TDT) patients. One hundred and three TDT patients (40.03 ± 9.15 years; 57.3% females) with serum ferritin < 2500 ng/mL were included. IO was assessed by T2* magnetic resonance imaging. Three groups were identified based on mean serum ferritin levels: <500 ng/mL (group 0; N = 32), 500–1000 ng/mL (group 1; N = 43), and 1000–2500 ng/mL (group 2; N = 28). All demographic and biochemical parameters were comparable among the three groups, with the exception of the triglycerides being significantly lower in group 0 than in group 2. No difference was found in the frequency of hepatic, endocrine, and cardiac complications. Hepatic IO was significantly less frequent in group 0 versus both groups 1 and 2. No patient with a serum ferritin level < 500 ng/mL had significant myocardial IO and alterations in the main hematological parameters. No difference in the distribution of the different chelation regimens was found. Serum ferritin < 500 ng/mL appears to be achievable and safe for several TDT patients. This target is associated with the absence of significant cardiac iron and significantly lower hepatic IO and triglycerides that are well-demonstrated markers for cardiac and liver complications.

Predictive Value of Hematological Parameters in Non-ST Segment Elevation Myocardial Infarction and Their Relationship with the TIMI Risk Score

ABSTRACT Background: Hematological parameters, such as white blood cell count (WBC), mean platelet volume (MPV), neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and WBC to MPV ratio (WMR), could provide data in prognosis, risk stratification, and optimal management in patients with acute coronary syndromes. Aim: We aimed to investigate the prognostic value of hematological parameters and their relationship with the TIMI risk score in non-ST elevation myocardial infarction (NSTEMI) patients. Material and Methods: A total of 259 adult patients with NSTEMI were included in this retrospective and observational cohort study. During a 1-year follow-up period, the efficacy of the main hematological parameters in predicting major adverse cardiovascular events (MACE) and their correlation with the TIMI risk score was analyzed. Results: Among the 259 patients, 188 (72.6%) were male, and the mean age was 60.4 ± 11.9 years. MACE was observed in 60 patients (23.2%). Elevated baseline levels of WBC, neutrophils, NLR, PLR, and WMR were associated with MACE development throughout the 1-year follow-up. Moreover, WBC, WMR, and NLR were correlated with the TIMI risk score. When the predictive power of these parameters for MACE was evaluated by ROC analysis, the AUC values for WBC, WMR, and NLR were 0.670 (95% CI 0.590–0.750), 0.666 (95% CI 0.582–0.746), and 0.689 (95% CI 0.610–0.767), respectively. Conclusion: WBC, NLR, and WMR predicted MACE in NSTEMI patients and were consistent with the TIMI risk score. On this basis, they could provide supportive data for early risk stratification and optimized therapeutic approach, particularly in high-risk patients.

Physiological changes in the blood of calves by plant food when using Katozal

An increase in livestock productivity is closely linked to an increase in calf vitality. This can be achieved using a number of approaches, including through the use of a number of biological stimulants in calves. The effectiveness of their use can be determined during the assessment of the main hematological parameters. As a result of 7 courses of daily use of 15.0 ml of Katozal plant nutrition in calves per animal, physiologically optimal changes in blood parameters were achieved. The results obtained as a result of regular injections of Katozal are regarded as highly physiologically beneficial for increasing the viability of animals and increasing their productive potential. The achieved dynamics of hematological parameters can be considered as a marker of the formation in the body of calves under the influence of Katozal of the situation, contributing to a pronounced increase in the processes of anabolism. The use of the drug Katozal is very promising for biological stimulation of calves at the end of early ontogenesis.