Main Components Of Oil Research Articles

On the clustering of triacylglycerols in the molten state

The liquid–solid phase transition of triacylglycerols (TAGs), the main components of edible fats and oils is central to the production and sensory properties of many processed foods. While there has been extensive research on the nucleation and growth of fats, there remains a dearth of knowledge regarding the structural organization of TAGs in the liquid state. From a molecular perspective, TAGs consist of three alkyl chains esterified to a glycerol backbone. Several models based on experiment and simulation have helped to unveil TAG organization in the molten state. However, more evidence for their structural organization is necessary. Here, we provide simulation and experimental insights on the structural organization of molten tripalmitin using small-angle neutron and x-ray scattering, and wide-angle x-ray scattering. In agreement with recent work, we also propose a model in which TAGs associate as clusters via glycerol-glycerol interactions, with their alkyl chains extending outwards in a loose shell. Our model, however, highlights and demonstrates the dynamic nature of clusters, where TAGs can transfer from one cluster to another via diffusion. The average number of TAG molecules per cluster varies from 5 to 9 and decreases with increasing temperature, which results in a smaller average distance between clusters. Overall, this study strongly suggests that prior to the onset of nucleation, TAGs are associated as dynamic clusters formed via intermolecular interactions between neighboring glycerol cores.

Kinetics of rancidity: 1H-NMR fingerprinting-based photodegradation monitoring of citronella oil

ABSTRACT Although known as a natural mosquito repellent, especially in the tropical region, citronella oil has many other therapeutic properties including antimicrobial and antifungal. Citronella oil can be applied in pure form or as a component in different cosmetic products. As with other essential oils, citronella has specific storage conditions to prevent its rancidity. 1H-NMR fingerprinting was used to monitor the changes in the concentration of citronellal, the main component of citronella oil. Quantitative analysis of the 1H-NMR spectral data digitized the degradation pattern of citronellal to determine the effect of sunlight exposure. Significant spectral changes were observed in clear glass vials starting from the 7th day of sun exposure. The degradation of citronellal followed first-order kinetics with a half-life of 15.7 ± 2.9 days. Moreover, the effect of sunlight exposure to citronellal is highly apparent in clear glass vials than in amber glass vials. The results of this study supported the general storage condition for essential oils to maximize shelf life.

Phytochemical constituents of bark essential oils of Cinnamomum zeylanicum Blume and effects on liver tissue of rats

Cinnamomum zeylanicum Blume is an evergreen tropical tree belonging to the Lauraceae family; it is a useful plant in traditional and modern medicines. Essential oils of Cinnamomum zeylanicum were prepared and identified using gas chromatography–mass spectrometry (GC-MS). For histological study 80 adult male rats were employed in this study. It is the same weight (30 ±5 gm) and 75 days in age. The animals were divided into 4 groups, with ten rats in each group. The results indicated that C. zeylanicum bark contained 27 different phytochemical components. The main components included Cinnamaldehyde (2-Propenal, 3-phenyl-) (46.46%), 9-Methoxybicyclo, [6.1.0] nona-2,4,6-triene (31.31%), alpha-, Muurolene (7.14%) and Copaene (1.63%). Sections of livers were examined for histological abnormalities, such as the breakdown of the liver architecture and increased inflammation, in comparison to control samples, some hepatocytes were swollen with pyknotic, large nuclei and dilated sinusoids. As well as in the rats treated with noticed sever congestion of central vein (CV), degeneration and sluggish of endothelial lining larger, Bile ducts hyperplasia and necrotic hepatocytes. It can be deduced that the main component of cinnamon bark oil is cinnamaldehyde and the liver displayed some tissue changes, including hepatocyte necrosis and the existence of steatosis foci. Histological and cellular changes in liver tissue were dramatically attenuated by C. zeylanicum essential oil.

ReaxFF Molecular Dynamics Study on the Microscopic Mechanism for Kerogen Pyrolysis.

Kerogen is mainly composed of carbon, hydrogen, and oxygen, which are the main components of crude oil and gas. The pyrolysis of kerogen is an efficient method to generate clean energy. In the present work, the pyrolysis reaction process of three types of kerogen is simulated using ReaxFF molecular dynamics (MD) methods to study the microscopic mechanism and the distribution of products. The results indicated that the pyrolysis products of the three types of kerogen significantly depend on the molecular structures, temperature, and reaction time. As the temperature increases, the gaseous hydrocarbon and the light and heavy oil fractions decreased, where small molecular fragments polymerized to form new molecular fragments. For an isothermal temperature, with the reaction proceeding, some component polymerization of the pyrolyzed fragments occurred, resulting in the generation of new light oils and heavy oils. Moreover, quantum chemical analysis was employed to reveal the kerogen pyrolysis mechanism. First, the weak bonds such as C-O, C-N, and C-S structures were decomposed to generate large carbon and some heavy shale oil fragments. Second, the cycloalkanes and long-chain alkanes were decomposed to generate a large amount of light shale oil and gaseous hydrocarbons. Finally, the decomposition of C═C in the aromatic ring, the secondary decomposition of light and heavy shale oils, and the further decomposition of short-chain alkanes occurred. In addition, the production of hydrogen (H2) occurred at the late stage of the pyrolysis reaction. Hydrogen radicals were formed by the decomposition of C-H bonds and subsequently collided with each other, resulting in the formation of H2 molecules. The pyrolysis and chemical analysis of kerogen can clearly determine the type and content of hydrocarbon substances, providing scientific data for exploration, development, and utilization of shale gas and shale oil.

Component composition and biological activity of essential oil of plant Zinnia elegans

By the method of steam distillation, samples of essential oil were obtained from a plant cultivated in the East Kazakhstan region. The essential oil was obtained with a yield in terms of air-dry raw materials. The method of gas-liquid chromatography carried out a quantitative analysis of the components of the essential oil. The quantitative content of essential oil components was calculated from the areas of gas chromatographic peaks without using correction factors. The main components of Zinnia elegans essential oil are α-terpineol, β-myrcene, myrtenal and camphene. Terpineol has a pleasant, lilac-like odor and is a common ingredient in perfumes, cosmetics, and fragrances. Pleasant smelling oily liquid. Myrcene has a large number of beneficial properties for the body, whether pure or in aromatherapy through the essential oils that contain it: Sedative and muscle relaxant, anti-inflammatory, interferon inhibition, gastric and duodenal ulcers, antalgic and antinociceptive, reduces sensitivity to pain by self-healing endogenous derivatives of morphine. Myrtenal - has an anti-inflammatory, resolving effect on acne, infiltrates. Increases the protective barrier of the epidermis. Antioxidant, rejuvenating. Camphene components are effective in the treatment of fungal, viral and bacterial infections that affect the respiratory system.

Characterization of garlic oil/β-cyclodextrin inclusion complexes and application

Garlic oil is a liquid extracted from garlic that has various natural antibacterial and anti-inflammatory properties and is believed to be used to prevent and treat many diseases. However, the main functional components of garlic oil are unstable. Therefore, in this study, encapsulating garlic oil with cyclodextrin using the saturated co-precipitation method can effectively improve its chemical stability and water solubility and reduce its characteristic odor and taste. After preparation, the microcapsules of garlic oil cyclodextrin were characterized, which proved that the encapsulation was successful. Finally, the results showed that the encapsulated garlic oil still had antioxidant ability and slow-release properties. The final addition to plant-based meat gives them a delicious flavor and adds texture and mouthfeel. Provided a new reference for the flavor application of garlic cyclodextrin micro-capsules in plant-based meat patties.

Nutritional Value, Volatile Components, Functional Metabolites, and Antibacterial and Cytotoxic Activities of Different Parts of Millettia speciosa Champ., a Medicinal and Edible Plant with Potential for Development.

Highly nutritious traditional plants which are rich in bioactive substances are attracting increasing attention. In this study, the nutritional value, chemical composition, biological activities, and feed indices of different parts of Millettia speciosa were comprehensively evaluated. In terms of its nutritional value, this study demonstrated that the leaves, flowers and seeds of M. speciosa were rich in elements and amino acids; the biological values (BVs) of these ingredients ranged from 85% to 100%, showing the extremely high nutritional value of this plant. GC-MS analysis suggested that the main chemical components of the flower volatile oil were n-hexadecanoic acid (21.73%), tetracosane (19.96%), and pentacosane (5.86%). The antibacterial activities of the flower and seed extracts were significantly stronger than those of the leaves and branches. The leaf extract displayed the strongest antifungal activities (EC50 values: 18.28 ± 0.54 μg/mL for Pseudocryphonectria elaeocarpicola and 568.21 ± 33.60 μg/mL for Colletotrichum gloeosporioides) and were the least toxic to mouse fibroblasts (L929) (IC50 value: 0.71 ± 0.04 mg/mL), while flowers were the most toxic (IC50 value: 0.27 ± 0.03 mg/mL). In addition, the abundance of fiber, protein, mineral elements, and functional metabolite contents indicated the potential applicability of M. speciosa as an animal feed. In conclusion, as a traditional herbal plant used for medicinal and food purposes, M. speciosa shows potential for safe and multifunctional development.

A Review on Formulation Development using Chaulmoogra Oil

To investigate the many bacteriocidal properties of chaulmoogra oil, which are used to treat leprosy and TB. Chaulmoogric acid, an antibacterial substance, is the main active component of chaulmoogra oil. The common name Chaulmoogra is used to refer to the tree Hydnocarpus kurzii, which belongs to the Flacourtiaceae family. Thailand, Myanmar, and India are the native countries of this tree. The two main Indian states where this tree can be found are Assam and Tripura. The ripe seeds of plants like Taraktogenos kurzii King, also called Hydnocarpus kurzii (King) Warb., Hydnocarpus wightiana Blume, Hydnocarpus anthelminticta Pierre, Hydnocarpus heterophylla, and other species of Hydnocarpus that are members of the Flacourtiaceae family are used to make the fixed oil known as chaulmoogra. Chaulmoogra oil has powerful bactericidal effect against the bacteria that cause leprosy and tuberculosis because it contains unsaturated fatty acids. Leprosy, TB, gout, and skin disorders have all been treated with it for millennia in Ayurveda.

Critical assessment of the endocrine potential of Linalool and Linalyl acetate: proactive testing strategy assessing estrogenic and androgenic activity of Lavender oil main components.

The acyclic linear monoterpenes Linalool (Lin) and Linalyl acetate (LinAc) occur in nature as major constituents of various essential oils such as lavender oils. A potential endocrine activity of these compounds was discussed in literature including premature thelarche and prepubertal gynecomastia due to lavender product use. This study aims to follow-up on these critical findings reported by testing Lin and LinAc in several studies in line with current guidance and regulatory framework. No relevant anti-/ER and AR-mediated activity was observed in recombinant yeast cell-based screening tests and guideline reporter gene in vitro assays in mammalian cells. Findings in the screening test suggested an anti-androgenic activity, which could not be confirmed in the respective mammalian cell guideline assay. Mechanistic guideline in vivo studies (Uterotrophic and Hershberger assays) with Lin did not show significant dose related changes in estrogen or androgen sensitive organ weights and a guideline reproductive toxicity screening study did not reveal evident effects on sex steroid hormone sensitive organ weights, associated histopathological findings and altered sperm parameters. Estrous cycling and mating/fertility indices were not affected and no evident Lin-related steroid hormone dependent effects were found in the offspring. Overall, the initial concerns from literature were not confirmed. Findings in the yeast screening test were aberrant from follow-up guideline in vitro and in vivo studies, which underlines the need to apply careful interpretation of single in vitro test results to support a respective line of evidence and to establish a biologically plausible link to an adverse outcome.

Study of Esterification between Oleic Acid and 2-Ethyl Hexanol by using the Microwave Method

Oleic acid is an unsaturated fatty acid, has a rancid odor and the main component of palm oil. To increase the value of oleic acid which has a C18 chain, it has the potential as a raw material for making long chain fatty acid esters. This fatty acid ester from vegetable oil is an emollient ester which is biodegradable which was synthesized with 2-ethyl hexanol using the Microwave method. In the last ten years, the microwave method has been widely used in organic synthesis. This esterification reaction was carried out using a modified home microwave by making a hole on top as a place for the ball cooler equipped with a stirrer, and using an acid catalyst. Characteristics of long chain fatty acid esters will be tested by FTIR and GC-MS. FTIR test results showed absorption of specific functional groups, while GC-MS was able to show the presence of long chain fatty acid ester compounds.

Agarwood oil nanoemulsion counteracts LPS-induced inflammation and oxidative stress in RAW264.7 mouse macrophages

PurposeOxidative stress and inflammation are key pathophysiological features of chronic respiratory diseases, including asthma and chronic obstructive pulmonary disease (COPD). Agarwood oil obtained from Aquilaria trees has promising antioxidant and anti-inflammatory activities. However, its clinical application is hampered by poor solubility. A viable approach to overcome this involves formulation of oily constituents into emulsions. Here, we have investigated the antioxidant and anti-inflammatory potential of an agarwood oil-based nanoemulsion (DE’RAAQSIN) against lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages in vitro. MethodsThe antioxidant and anti-inflammatory activity of DE’RAAQSIN was assessed by measuring the levels of ROS and nitric oxide (NO) produced, using the DCF-DA assay and the Griess reagent assay, respectively. The molecular pathways activated by DE’RAAQSIN were investigated via qPCR. ResultsLPS stimulation of RAW264.7 cells increased the production of nitric oxide (NO) and ROS and resulted in the overexpression of the inducible nitric oxide synthase (iNOS) gene. Furthermore, LPS induced the upregulation of the expression of key proinflammatory genes (IL-6, TNF-α, IL-1β, and CXCL1) and of the antioxidant gene heme oxygenase-1 (HO-1). DE’RAAQSIN demonstrated potent antioxidant and anti-inflammatory activity by significantly reducing the levels of ROS and of secreted NO, simultaneously counteracting the LPS-induced overexpression of iNOS, IL-6, TNF-α, IL-1β, and HO-1. These findings were corroborated by in silico activity prediction and physicochemical analysis of the main agarwood oil components. ConclusionsWe propose DE’RAAQSIN as a promising alternative managing inflammatory disorders, opening the platform for further studies aimed at understanding the effectiveness of DE’RAAQSIN.

A Review of Taila Varga (Edible Vegetable Oils) According to Bruhat Trayi

Vegetable oils have been used for many centuries in traditional Ayurvedic medicine in India and in Chinese medicine for their therapeutic properties. The purpose of this article is to inform readers about the characteristics of edible oil as described in Ayurveda, with particular emphasis on Charak Samhita, Sushruta Samhita, and Ashtanga Hridaya. Literature about nutrition and dietetics was studied in books, journals, and research engines. The main components of vegetable oils are sesame, coconut, groundnut, sunflower, soybean, palm, safflower, and mustard oils. Many other oils used as essential oils or for medicinal purposes. In addition, many oils can be used as functional food.

Scaled experimental study on the initial oil thickness effect on the combustion characteristic of the typical transformer insulating oil

Compared with mineral oil, plant insulation oil has a higher flash point, excellent natural degradation ability, and higher electrical insulation. It is expected to become the main component of transformer insulation oil, but the fire safety of this oil needs to be further analyzed and improved. In this paper, a cone calorimeter was used to test the combustion of typical plant insulating oil (camellia seed oil, soybean oil) and mineral oil (25# mineral oil) under different radiant heat flows and initial oil thickness. The combustion parameters such as ignition time, heat release rate, and carbon generation rate were measured. The combustion characteristics of 25# mineral oil were greatly affected by radiative heat flow and oil thickness. The peak heat release rate and carbon generation rate of mineral oil under high heat flow (50 kW/m2) were significantly higher than those under low heat flow (25 kW/m2) and increased with the increase of initial oil thickness. Plant insulating oil (camellia seed oil and soybean oil) had similar laws, but the peak value of the heat release rate and carbon generation rate of soybean oil were relatively small. Petrella fire risk assessment method showed that the fire risk ranking in descending order was mineral oil, camellia seed oil, and soybean oil, which provided a reference for the further popularization and application of plant insulation oil transformer.

The protective effect of hydroxytyrosol on the heart in rats fed corn syrup: The role of spexin, pentraxin-3

Background/Aim: Increased consumption of corn syrup has been linked to various metabolic diseases. The Mediterranean diet, one of the healthiest known diets, is renowned for its cardioprotective effects. We investigated the possible roles of new molecules such as spexin (SPX) and pentraxin-3 (PTX-3) in the protective effect of hydroxytyrosol (HT), one of the primary main components of olive oil, in rats fed corn syrup. Methods: The animals were divided into four groups of n=6 rats each: Group I (Control), Group II (HT), Group III (Corn Syrup), and Group IV (Corn Syrup+HT). The rats were given 30% corn syrup with drinking water for 6 weeks. Liquid containing HT (4 ml/kg/day) was applied by oral gavage alone and together with corn syrup for 6 weeks. SPX and PTX-3 were examined histopathologically in the animals’ heart tissue after the rats were sacrificed, and histoscores were created for SPX and PTX-3 immunoreactivity. The data were analyzed using both one-way ANOVA and Tukey’s HSD test. Results: We detected a decrease in SPX (P<0.001) and an increase in PTX-3 (P=0.013) in the Corn Syrup group compared with the Control group. After HT treatment, an increase in SPX (P=0.025) and a decrease in PTX-3 (P<0.001) were detected. There were no differences between the HT and Control groups. Conclusion: The protective effects of HT against heart damage might be conferred via SPX and PTX-3. These molecules are considered to be important target molecules involved in the diagnosis and treatment of metabolic diseases.

Network pharmacology on mechanistic role of Thymus linearis Benth. against gastrointestinal and neurological diseases

BackgroundThymus linearis Benth. (TL) is native to the Himalayas and has been used traditionally to cure various diseases. Thymus is a well-known aromatic perennial herb commonly known as Van ajwain or Himalayan Thyme. The main components of the TL essential oil are thymol, terpinene, and p-cymene. There are many biological properties that TL has been reported to exhibit, including antioxidant, analgesic, anti-inflammatory, antipyretic, antibacterial, anticancerogenic, and neuroprotective effects. PurposeIn this study, the network pharmacology and molecular docking were used to explore the potential compounds of TL and their interaction mechanism toward gastrointestinal complications and neurological diseases. MethodsNetwork pharmacology was used to analyze the active compounds and protein targets of TL on gastrointestinal and neurological related diseases. Protein-protein interaction and Kyoto Encyclopaedia of Genes and Genomes analysis were used to enrich and evaluate key pathways of target proteins. To ensure the reliability of the network pharmacology prediction estimates, molecular docking was used to confirm the relationships between the core components and targets of TL. Result77 physiologically active compounds and their 717 predicted protein targets with high association to the neuroactive ligand-receptor interaction pathway were derived from TL. Beta-citronellol, piperitol, p-cymen-8-ol, and alpha-humulene were found to have a role in gastrointestinal diseases associated with neurological diseases. These compounds showed significant levels of multitargeting cluster regulatory activity. The molecular docking results showed regulatory activity of highlighted multi-targeting compounds and the highest docking energy was reported in piperitol. ConclusionThe study shows that Thymus linearis Benth., a medicinal plant with traditional use, possesses valuable biologically active compounds. It emphasizes the potential of Thymus in treating gastrointestinal and neurological diseases by regulating oxidative stress pathways. This research opens up possibilities for discovering antioxidant molecules for future drug development. It is an interesting study with promising implications for further research.

Penerapan Aromaterapi Lavender Terhadap Penurunan Nyeri Pada Pasien Post Sectio Caesarea Di RSUD Kardinah Tegal

Sectio is a surgical operation to help pregnant women give birth by making an incision in the abdominal wall (laparotomy) and uterus (hysterectomy). Compared with regular births, which only cause about 9% more suffering, births by cesarean section cause about 27.3% more pain. Pharmacology and non-pharmacology can be used together to treat pain. Within 24 hours after a caesarean section, patients can experience a five times greater reduction in pain thanks to lavender aromatherapy. There are no risks associated with using the main active components of lavender oil, linalyl acetate, and linalool, which may provide a calming effect. When lavender aromatherapy is inhaled, the compounds it contains trigger the hypothalamus to produce endorphins which can calm and soothe. Lavender aromatherapy also has properties that make it antibacterial, analgesic, antidepressant and antispasmodic. By offering and using lavender aromatherapy, this research approach is descriptive and takes the form of a case report. The aim of this research is to determine whether the use of lavender aromatherapy can help patients after giving birth by caesarean section reduce their discomfort. After receiving lavender aromatherapy, the research findings changed from a scale of 7 to 3. Research findings show that the use of lavender aromatherapy can help postpartum patients undergoing cesarean section reduce their discomfort.

P09-09: Experimental evidence for the absence of (anti-)estrogenic and (anti-)androgenic activities of the lavender essential oil main components linalool and linalyl acetate

P09-09: Experimental evidence for the absence of (anti-)estrogenic and (anti-)androgenic activities of the lavender essential oil main components linalool and linalyl acetate

Enhanced Two-Step Extraction from Biomass of Two Cymbopogon Species Cultivated in Santander, Colombia.

The insertion of circular economy principles into the essential oil (EO) production chain aims to reduce waste generation and make integral use of harvested plant material. Higher profits from integral use with reduced waste generation contribute to the eventual use of the EO value chain as an alternative to illicit crops in Colombia (mostly coca). In this study, Java-type citronella (Cymbopogon winterianus) and palmarosa (C. martinii) plant materials were used in two consecutive processes to obtain EOs and extracts. The residual biomass after EO distillation was subjected to ultrasound-assisted hydroethanolic extraction to afford extracts that contained bioactive compounds. Citronella and palmarosa were distilled with typical EO yields (1.0 ± 0.1% for citronella; 0.41 ± 0.06% for palmarosa; n = 5) either through hydrodistillation assisted by microwave radiation or through steam distillation, and their composition (determined via GC/FID/MS analysis) and physicochemical parameters fell within their ISO standard specifications. The concentration of citronellal, the major compound of citronella oil, was 500 ± 152 mg/g. Geraniol, the main component of palmarosa oil, was found at 900 ± 55 mg/g. The citronella and palmarosa hydroalcoholic extracts (4-11% yield) were analyzed with UHPLC-ESI-Orbitrap-MS, which permitted the identification of 30 compounds, mainly C-glycosylated flavones and hydroxycinnamic acids. Both extracts had similar antioxidant activity values, evaluated using the ABTS+● and ORAC assays (110 ± 44 µmol Trolox®/g extract and 1300 ± 141 µmol Trolox®/g extract, respectively).

Rheological Study of the Formation of Pullulan Hydrogels and Their Use as Carvacrol-Loaded Nanoemulsion Delivery Systems.

Hydrogels have been extensively studied as delivery systems for lipophilic compounds. Pullulan hydrogels were prepared, and their gelation kinetics were studied over time. Pullulan exhibited a relatively slow gelling reaction in basic medium (KOH) using trisodium metaphosphate (STMP) as a cross-linking agent, so capsules cannot be obtained by dripping as easily as in the case of alginate and chitosan. The kinetics of pullulan gelation were studied through rheological analysis over time. An optimal [Pullulan]/[KOH] ratio was found for a fixed [Pullulan]/[STMP] ratio. For this given relationship, gelling time measurements indicated that when the concentration of pullulan increased, the gelation time decreased from 60 min for 6% w/w pullulan to 10 min for 10% w/w. After the gel point, a hardening of the hydrogel was observed over the next 5 h. The formed hydrogels presented high degrees of swelling (up to 1800%). Freeze-dried gels were capable of being rehydrated, obtaining gels with rheological characteristics and visual appearance similar to fresh gels, which makes them ideal to be freeze-dried for storage and rehydrated when needed. The behavior of the hydrogels obtained as active ingredient release systems was studied. In this case, the chosen molecule was carvacrol (the main component of oregano oil). As carvacrol is hydrophobic, it was incorporated into the droplets of an oil-in-water nanoemulsion, and the nanoemulsion was incorporated into the hydrogel. The release of the oil was studied at different pHs. It was observed that as the pH increased (from pH 2 to pH 7), the released amount of carvacrol for the gel with pullulan 10% w/w reached 100%; for the other cases, the cumulative release amount was lower. It was attributed to two opposite phenomena in the porous structure of the hydrogel, where more porosity implied a faster release of carvacrol but also a higher degree of swelling that promoted a higher entry of water flow in the opposite direction. This flow of water prevented the active principle from spreading to the release medium.

The Effect of Temperature on the Size and the Deposition of Asphaltene Particles

Summary The deposition of asphaltene as a main component of crude oil is considerably affected by temperature. Despite the studies on influencing factors on deposition and size of asphaltene particles, no experimental research was previously conducted on the simultaneous impact of temperature on asphaltene particle size and deposition. In this study, the asphaltene deposit mass was measured within a Couette device at various temperatures ranging between 20 and 65°C under a constant angular velocity. Furthermore, the asphaltene particle size was simultaneously measured to investigate the relationship between deposition mass and asphaltene particle size and also to validate the concept of critical particle size. A digital microscope was used to measure the size of unstable asphaltene particles in oil. Asphaltene solubility and oil viscosity were measured to understand the deposition mechanisms. The analysis of microscopic images indicated that larger asphaltene particles are produced at higher temperatures. Although the total mass of the deposit was decreased with temperature, the deposition fraction, defined as mass fraction of total unstable asphaltene particles that deposit, was increased. Higher fraction of deposition was found for larger particles that is in contradiction to the previously introduced critical particle size concept. Additionally, the effect of solubility was found to be dominant in comparison with viscosity from the point of view of the total mass of the deposit. For the oil sample investigated in this study, a 45°C increase in temperature reduced the total mass of the deposit by 46.84%.