This study investigates the effects of complete slip conditions on the peristaltic pumping of a Casson nanofluid with suction and injection in a vertical due to the crucial role that nano liquids play in a variety of technological and medical fields, particularly in peristalsis, a mechanism that transports liquids. The Casson fluid belongs to a class of non-Newtonian fluids that, through a particular stress threshold magnitude, exhibit elastic solid behavior before changing to liquid behavior. These fluids have several uses in engineering, food preparation, drilling and other fields. After establishing the governing conservation equations, the resulting flow model is effectively simulated using the realistic assumptions of a long wavelength and a low Reynolds number. The temperature distributions, velocity, pressure rate per wavelength and nanoparticle concentration of the resulting flow problem have been solved analytically. The effects of all physical factors on temperature, velocity, concentration fields, pressure rate, frictional force and pressure gradient are graphically examined using Wolfram MATHEMATICA software. There are a variety of biofluids that cannot be classified as liquids. For example, blood contains WBC, RBC and plasma. It is essential to model biofluids (blood) as nanofluids given the physical properties of these biofluids. According to reports, one of the finest yield stress models is the Casson model, and blood exhibits a similar behavior. We took these facts into consideration when thinking about Casson nanofluid flow in a vertical layer under peristalsis. Additionally, the suction and injection mechanisms can be used to represent the exchange of carbon dioxide in bold. In order to understand how blood flows through small blood vessels, this model must be examined. The obtained results show that the Newtonian case and those found in the literature have a very good agreement. Since the liquid moves faster and more effectively when the value is increased, it becomes clear that this increases the strength of the velocity. In other words, nanoperistaltic pumps can maintain a pressure differential that increases or decreases at all operating flow rates with an increasing thermophoresis effect. Furthermore, it is obvious that the pressure reduction in a Casson fluid is greater than in a Newtonian fluid.