When blood comes in contact with dialyzer membranes, the interfacial bioactivation reactions occurring at blood/membrane boundaries detect the gross mechanism of thrombogenicity. In the presence of biocompatible polymers, the adhesion and subsequent interactions of platelets, as well as other serum components (e.g., proteins), are altered. It delays thrombus formation. In this study, the hemocompatibility of polyvinylpyrrolidone (PVP) modified polyethersulphone (PES) hemodialysis membrane fabricated using UV-assisted photochemical synthesis was investigated. To obtain varying degrees of PVP modification on each membrane, each coated surface was aged within the viscous polymer suspension for a predetermined duration. The correlation between the membrane induced hemocompatibility with membrane roughness, chemical composition, and surface charge at different aging duration of coating. In our study, we performed a comprehensive investigations on membrane morphology, hydrophilicity and its chemical composition using Atomic Force Microscopy (AFM), Fourier Transform Infra-Red (FTIR) Spectroscopy, Nuclear Magnetic Resonance (NMR), Zeta potential, and Scanning Electron Microscopy (SEM). The coated samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, C5a, 1L-1α, 1L-1β, IL6, and C5b-9. Modified membranes showed significant resistance against fibrinogen adsorption compared to their bare membrane counterpart. This inherent antifouling activity against fibrinogen adsorption was observed more on modified membranes that aged longer within the PVP suspension during synthesis. A longer ageing period contributed to the enhanced PVP modification, hence, more stable polymeric hydration layers. In addition, both bare and coated membranes triggered both complement system activation and inflammatory responses, but to a mild degree in the latter case. Coated membranes at longer coating duration, around ∼4 min, had smoother surfaces and lower magnitudes of surface charge and consequently exhibited reduced release of all biomarkers (except IL-1α), indicating enhanced biocompatibility. Higher levels of C5a were observed in membranes coated at 1 and 2 min, promoting modest reduction toward the release of other components.