Magnetic Tracer Research Articles

Intraoperative localization of gastrointestinal tumors by magnetic tracer technique during laparoscopic‐assisted surgery (with video)

Background Laparoscopic localization of gastrointestinal tumors has long been an important objective. This study aimed to evaluate the clinical application of a magnetic tracer technique during laparoscopic-assisted surgery. Methods Fifty-seven patients with gastrointestinal tumors, who voluntarily underwent endoscopic marking between May 2019 and May 2020, were enrolled. A magnetic ring was clamped onto tissues adjacent to the lesion and released during preoperative endoscopy. Then, another magnet ring or laparoscopic instrument was delivered to the wall of the digestive tract contralateral to the lesion and attracted, thus achieving accurate intraoperative localization. Observational evaluation included data regarding preoperative marking, intraoperative localization, operation, and safety. Results Fifty-six of the 57 (98.2%) patients with gastric tumors (n = 35), duodenal tumors (n = 1), and colorectal tumors (n = 20), successfully underwent marking, localization, and resection. The mean margins of proximal and distal resection of colorectal tumors were 106 and 78 mm, respectively. The mean (± SD) duration of endoscopic marking and laparoscopic localization for gastric/duodenal and colorectal tumors were 5.3 ± 0.3, 1.0 ± 0.1, 5.5 ± 0.4, and 1.0 ± 0.1 min, respectively. No complications occurred in 56 of the 57 patients. Conclusions The magnetic tracer technique demonstrated promising potential as a localization method for gastrointestinal tumors, with superior safety, effectiveness, rapidity, and convenience.

Optimization of SPIO Injection for Sentinel Lymph Node Dissection in a Rat Model.

Simple SummaryIn this study, the following injection characteristics were evaluated to optimize magnetic tracer uptake in the sentinel lymph nodes (SLN) in a rat hindleg model: (a) iron dose, (b) effect of dilution, (c) effect of injecting at different time courses and (d) effect of massaging the injection site. In conclusion, injection dose and time were primary factors for the SLN iron uptake. The result from this study will provide a background for magnetic procedures.The magnetic technique, consisting of a magnetic tracer and a handheld magnetometer, is a promising alternative technique for sentinel lymph node dissection (SLND) and was shown to be non-inferior to the standard technique in terms of identification rates. In this study, injection characteristics (iron dose, dilution, time course and massaging) were evaluated to optimize magnetic tracer uptake in the sentinel lymph nodes (SLN) in a rat hindleg model. 202 successful SLNDs were performed. Iron uptake in the SLN is proportional (10% utilization rate) to the injection dose between 20 and 200 μg, showing a plateau uptake of 80 μg in the SLN around 1000 μg injection. Linear regression showed that time had a higher impact than dilution, on the SLN iron uptake. Massaging showed no significant change in iron uptake. The amount of residual iron at the injection site was also proportional to the injection dose without any plateau. Time was a significant factor for wash-out of residual iron. From these results, preoperative injection may be advantageous for SLN detection as well as reduction in residual iron at the injection site by potential decrease in required injection dose.

Short-term variations of surface magnetism and prominences of the young Sun-like star V530 Per

Aims. We investigate magnetic tracers in the photosphere and the chromosphere of the ultra-rapid rotator (P ~ 0.32 d) V530 Per, a cool member of the open cluster α Persei, to characterize the short-term variability of the magnetic activity and large-scale magnetic field of this prototypical young, rapidly rotating solar-like star. Methods. With time-resolved spectropolarimetric observations spread over four close-by nights, we reconstructed the brightness distribution and large-scale magnetic field geometry of V530 Per through Zeeman-Doppler imaging. Simultaneously, we estimated the short-term variability of the surface through latitudinal differential rotation. Using the same data set, we also mapped the spatial distribution of prominences through tomography of Hα emission. Results. As in our previous study, a large dark spot occupies the polar region of V530 Per with smaller, dark, and bright spots at lower latitudes. The large-scale magnetic field is dominated by a toroidal, mostly axisymmetric component. The maximal radial field strength is equal to ~1 kG. The surface differential rotation is consistent with a smooth Sun-like shear dΩ = 0.053 ± 0.004 rad d−1, close to the solar shear level. The prominence pattern displays a stable component that is confined close to the corotation radius. We also observe rapidly evolving Hα emitting structures, over timescales ranging from minutes to days. The fast Hα evolution was not linked to any detected photospheric changes in the spot or magnetic coverage.

Sentinel lymph node biopsy in breast cancer after neoadjuvant therapy using a magnetic tracer versus standard technique: A multicentre comparative non-inferiority study (IMAGINE-II)

IntroductionPrevious studies have shown that a magnetic tracer technique using superparamagnetic iron oxide (SPIO) and a manual magnetometer (Sentimag, SM) is as effective as the standard technique using a radioisotope injection and a gamma probe (GP) for the detection of sentinel lymph nodes (SLNs) in breast cancer (BC) patients. This study was designed to investigate the performance of SM for post-neoadjuvant (NAT) SLN biopsy in BC patients. Materials and methodsPost-NAT BC patients were recruited from five centres. Readings of SLNs were recorded in transcutaneous, intraoperative and ex vivo scenarios by both GP and SM techniques. SLNs were assessed by OSNA (One-Step Nucleic Acid Amplification). ResultsA total of 89 patients were included. At the patient level, the transcutaneous and intraoperative SLN detection rate was 97.8% by both techniques. At the node level, the GP detection rate intraoperatively was lower than that of SM (93.8% vs. 99.2%), with a concordance rate of 93% (90% CI 1.25; 9.44). The ex vivo detection rate was lower for GP compared to SM both per patient 96.6% vs. 97.8%, and per node 90.6% vs. 98.4% (90% CI -2.03; 4.22 and 1.82; 13.68, respectively). Furthermore, the detection rate of pathologically positive SLNs per patient and per node was lower for GP than SM both intraoperatively and ex vivo. These results showed the non-inferiority of SM intraoperatively per node (90% CI -4.89; 20.89) and ex vivo per patient (90% CI -2.38; 29.66). ConclusionOur study showed the non-inferiority of SM compared to GP for detecting SLNs in post-NAT BC patients.

A complete magnetic sentinel lymph node biopsy procedure in oral cancer patients: A pilot study

ObjectivesTo assess the feasibility and merits of a complete magnetic approach for a sentinel lymph node biopsy (SLNB) procedure in oral cancer patients. Materials and methodsThis study included ten oral cancer patients (stage cT1-T2N0M0) scheduled for elective neck dissection (END). Superparamagnetic iron oxide nanoparticles (SPIO) were administered peritumorally prior to surgery. A preoperative MRI was acquired to identify lymph nodes (LNs) with iron uptake. A magnetic detector was used to identify magnetic hotspots prior, during, and after the SLNB procedure. The resected sentinel LNs (SLNs) were evaluated using step-serial sectioning, and the neck dissection specimen was assessed by routine histopathological examination. A postoperative MRI was acquired to observe any residual iron. ResultsOf ten primary tumors, eight were located in the tongue, one floor-of-mouth (FOM), and one tongue-FOM transition. SPIO injections were experienced as painful by nine patients, two of whom developed a tongue swelling. In eight patients, magnetic SLNs were successfully detected and excised during the magnetic SLNB procedure. During the END procedure, additional magnetic SLNs were identified in three patients. Histopathology confirmed iron deposits in sinuses of excised SLNs. Three SLNs were harboring metastases, of which one was identified only during the END procedure. The END specimens revealed no further metastases. ConclusionA complete magnetic SLNB procedure was successfully performed in eight of ten patients (80% success rate), therefore the procedure seems feasible. Recommendations for further investigation are made including: use of anesthetics, magnetic tracer volume, planning preoperative MRI, comparison to conventional technique and follow-up.

P-20 Magnetic tracer uptake within sentinel lymph nodes, an oral cancer patient cohort

P-20 Magnetic tracer uptake within sentinel lymph nodes, an oral cancer patient cohort

Magnetic nanoparticle technique versus radioisotope technique in detection of sentinel lymph node in early breast cancer: a systematic review and meta-analysis

Sentinel lymph node biopsy is the standard technique to stage the axilla in early breast cancer. The gold standard is the dual technique of radioisotope and blue dye injection. The drawbacks of dual technique include handling and disposal of radioactive material. Equally reliable, user and patient friendly, magnetic tracer super paramagnetic iron oxide, was compared with the radioisotope tracer in detection of Sentinel lymph node in a clinically node negative axilla in early breast cancer in this meta-analysis, with emphasis on the utility and safety of both techniques. PubMed, Medline were searched from April 2015 to October 2019. 1395 patients’ data was included from seven homogenous studies in this meta-analysis. A statistical analysis was performed using STATA 16.1 version for sentinel lymph node detection rate using standard technique, magnetic tracer technique and both techniques. Ratio of successes and failures of the outcomes was measured and analysed. A paired two sample ‘Z’ test was performed to compare between the standard technique and magnetic tracer techniques. The standardised mean difference of ratio of success rate between two techniques was; 1.013334 with a p value of 0.3136.The standardised mean difference of ratio of failure rate between two techniques was 1.016667, with a p value of 0.3132. Success and failure rate showed statistically no significant difference between the two techniques. This two-way analysis with paired two sample ‘Z’ test confirms that neither standard technique nor magnetic tracer techniques are superior or inferior to each other.

Synthesis and characterization of NiFe2O4 magnetic nanoparticles with different coating materials for magnetic particle imaging (MPI)

Magnetic nanoparticles (MNPs) are widely used in biomedical applications. Biocompatibility and superparamagnetic response of the MNPs make them viable, especially as contrast agents in magnetic resonance imaging (MRI) and tracer agents in magnetic particle imaging (MPI). Nickel ferrites (NiFe2O4) coated with citric acid (CA) and polyacrylic acid (PAA) were synthesized by hydrothermal process. Nickel ferrites were structurally characterized by fourier transformed infrared spectroscopy (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), and thermogravimetric analysis (TGA). Nickel ferrites were also magnetically evaluated with electron-spin resonance (ESR), and physical properties measurement system (PPMS). The samples crystallite size of 9–12 nm achieved with XRD is in good agreement with the magnetic domain size that was obtained by PPMS results. Furthermore, nickel ferrites were also evaluated with magnetic particle spectroscopy (MPS) to determine the expected performance in MPI scanner. MPS results of NiFe2O4@PAA showed minimum relaxation time and high resolution (FWHM, mT) as compared to Vivotrax (magnetic insight) and Perimag (micromod) commercial reference tracers used in MPI. All results show that nickel ferrites have a great potential to become an optimum tracer agent for MPI.

Heating ability modulation by clustering of magnetic particles for precision therapy and diagnosis

Magnetic and thermal properties of clustered magnetite nanoparticles submitted to a high-frequency magnetic field is studied by means of rate equations. A simple model of large particle clusters (containing more than one hundred individual particles) is introduced. Dipolar interactions among clustered particles markedly modify shape and area of the hysteresis loops in a way critically dependent on particle size and cluster dimensions, thereby modulating the power released as heat to a host medium. For monodisperse and polydisperse systems, particle clustering can lead to either a significant enhancement or a definite reduction of the released power; in particular cases the same particles can produce opposite effects in dependence of the dimensions of the clusters. Modulation by clustering of the heating ability of magnetic nanoparticles has impact on applications requiring optimization and accurate control of temperature in the host medium, such as magnetic hyperthermia for precision therapy or fluid flow management, and advanced diagnostics involving magnetic tracers.

Observational Signatures of Cosmic-Ray Interactions in Molecular Clouds

Abstract We investigate ionization and heating of gas in the dense, shielded clumps/cores of molecular clouds bathed by an influx of energetic, charged cosmic rays (CRs). These molecular clouds have complex structures, with substantial variation in their physical properties over a wide range of length scales. The propagation and distribution of CRs is thus regulated accordingly, in particular, by the magnetic fields threaded through the clouds and into the dense regions within. We have found that a specific heating rate reaching 10−26 erg cm−3 s−1 can be sustained in the dense clumps/cores for Galactic environments, and this rate increases with CR energy density. The propagation of CRs and heating rates in some star-forming filaments identified in IC 5146 are calculated, with the CR diffusion coefficients in these structures determined from magnetic field fluctuations inferred from optical and near-infrared polarizations of starlight, which is presumably a magnetic field tracer. Our calculations indicate that CR heating can vary by nearly three orders of magnitude between different filaments within a cloud due to different levels of CR penetration. The CR ionization rate among these filaments is similar. The equilibrium temperature that could be maintained by CR heating alone is of order 1 K in a Galactic environment, but this value would be higher in strongly star-forming environments, thus causing an increase in the Jeans mass of their molecular clouds.

Assessment of magnetite as a magnetic tracer for sediments in the study of ephemeral gully erosion: Conditioning factors of magnetic susceptibility

AbstractIn gully erosion, the detached soil can be transported over long distances along the landscape. The eroded material can be redistributed and/or deposited on the soil surface along the landscape and then eventually be buried by newly eroded and deposited sediment. There can be significant variability of the soil conditions (e.g., texture and moisture content) over which the eroded material travels. The eroded material can be detected through the use of magnetic tracers attached to or mixed with the eroded soil. In this study we evaluated the degree to which the magnetic signal of the magnetite is conditioned by (i) burial depth of tracer, (ii) condition of soil covering the tracer and (iii) tracer concentration. In the laboratory containers were filled with a specific soil. In the filling process, a 0.5‐cm layer of a soil–magnetite mixture was interspersed in the soil profile at a certain depth. Experiments encompassed three different soil–tracer concentrations (1000:1, 200:1, 100:1), four burial depths of tracer (0 cm, 3 cm, 5 cm and 10 cm from soil surface), and two different soils. In each case, the magnetic susceptibility was measured with a susceptometer. Experiments were repeated with different soil moisture contents. If the tracer is located under the soil surface, a minimum soil–tracer concentration of 200:1 is required for its correct detection. The intensity of the magnetic signal decreases dramatically with the vertical distance of the tracer from the soil surface. The maximum detection depth for the tracer's magnetic signal is strongly dependent on the natural magnetic susceptibility of the soil, which masks the tracer's signal. Variation in soil moisture content does not significantly affect the magnetic signal. For extensive field studies, the soil–tracer volume to be handled would be very high and therefore, it is necessary to explore new tracer application techniques.

Assessment of magnetite as a sediment tracer in the study of ephemeral gully erosion: Application and distribution in the soil

AbstractThere are few works where magnetic tracers are used for the assessment of water erosion, focusing on the traceability of sediments over the soil surface and in small areas (i.e., for laminar erosion survey). The main limitation of magnetic tracers is associated with its preparation and application, as the tracer is typically mixed with a large amount of soil and then spread manually over experimental sites susceptible to erosion. In gully erosion studies, the experimental sites (field areas where a gully is expected to develop) can be quite large. Consequently, the high volume/weight of the material (soil + tracer) to be prepared, transported, and applied renders this technique unfeasible. Moreover, eroded channels can reach a depth of several centimeters, requiring the tracer to be (re)located in the soil profile ideally at this same depth. This work presents a novel technique for the application and distribution of magnetite in the soil profile to track sediments in gully erosion assessments. Experiments were carried out in microplots, where a mixture of magnetite and water was applied over the soil surface using a backpack sprayer. Redistribution of the tracer in the soil profile was carried out with different conventional tillage implements. The control plots were subjected to the same experiments, but using a soil–tracer mixture (conventional technique). The application technique proposed herein enables a correct application of magnetite over large areas, being a promising alternative to the conventional technique. Hoeing tillage implements, such as the rotavator, ensure an approximately homogeneous redistribution of tracer in the soil profile when good soil moisture content is present.

Unravelling the origin of magnetic fields in galaxies

ABSTRACT Despite their ubiquity, there are many open questions regarding galactic and cosmic magnetic fields. Specifically, current observational constraints cannot rule out whether magnetic fields observed in galaxies were generated in the early Universe or are of astrophysical nature. Motivated by this, we use our magnetic tracer algorithm to investigate whether the signatures of primordial magnetic fields persist in galaxies throughout cosmic time. We simulate a Milky Way-like galaxy down to z ∼ 2–1 in four scenarios: magnetized solely by primordial magnetic fields, magnetized exclusively by supernova (SN)-injected magnetic fields, and two combined primordial + SN magnetization cases. We find that once primordial magnetic fields with a comoving strength B0 > 10−12 G are considered, they remain the primary source of galaxy magnetization. Our magnetic tracers show that, even combined with galactic sources of magnetization, when primordial magnetic fields are strong, they source the large-scale fields in the warm metal-poor phase of the simulated galaxy. In this case, the circumgalactic medium and intergalactic medium can be used to probe B0 without risk of pollution by magnetic fields originated in the galaxy. Furthermore, whether magnetic fields are primordial or astrophysically sourced can be inferred by studying local gas metallicity. As a result, we predict that future state-of-the-art observational facilities of magnetic fields in galaxies will have the potential to unravel astrophysical and primordial magnetic components of our Universe.

Detection of magnetic tracers with Mx atomic magnetometer for application to blood velocimetry

In the new generation of blood velocimeter systems, considerable attention has been paid to atomic magnetometers due to their high resolution and high sensitivity for detection of magnetic tracers. Passing the magnetic tracers adjacent to the atomic magnetometer produces a spike-like signal, the shape of which depends on the position of the tracer, as well as its velocity and orientation. The present study aimed to evaluate the effect of abrupt variations in the instantaneous velocity of the magnetic tracer on the magnetometer response compare to constant velocity. Modeling the magnetic tracer as a dipole moment indicated that the velocity dependence of the magnetic field and local magnetic field gradient associated with moving magnetic tracer cause the spike-like signal to go out of symmetry in the case of variable velocity. Based on the experimental results, any instantaneous variation in tracer velocity leads to shrinkage in the signal width. The behavior has been studied for both magnetic microwire with variable instantaneous velocity and magnetic droplets in stenosis artery phantom. In addition, the position of the tracer could be detected by following the shrinkage behavior which may occur on the peak, valley, or both. These advantageous outcomes can be applied for high sensitivity diagnosis of arterial stenosis.

Embracing Defects and Disorder in Magnetic Nanoparticles.

Iron oxide nanoparticles have tremendous scientific and technological potential in a broad range of technologies, from energy applications to biomedicine. To improve their performance, single‐crystalline and defect‐free nanoparticles have thus far been aspired. However, in several recent studies, defect‐rich nanoparticles outperform their defect‐free counterparts in magnetic hyperthermia and magnetic particle imaging (MPI). Here, an overview on the state‐of‐the‐art of design and characterization of defects and resulting spin disorder in magnetic nanoparticles is presented with a focus on iron oxide nanoparticles. The beneficial impact of defects and disorder on intracellular magnetic hyperthermia performance of magnetic nanoparticles for drug delivery and cancer therapy is emphasized. Defect‐engineering in iron oxide nanoparticles emerges to become an alternative approach to tailor their magnetic properties for biomedicine, as it is already common practice in established systems such as semiconductors and emerging fields including perovskite solar cells. Finally, perspectives and thoughts are given on how to deliberately induce defects in iron oxide nanoparticles and their potential implications for magnetic tracers to monitor cell therapy and immunotherapy by MPI.

Optimizing Dose and Timing in Magnetic Tracer Techniques for Sentinel Lymph Node Detection in Early Breast Cancers: The Prospective Multicenter SentiDose Trial.

Simple SummarySuperparamagnetic iron oxide (SPIO) nanoparticles have comparable performance to the combination of radioisotope and blue dye (RI + BD) for sentinel lymph node (SLN) biopsy in breast cancer. In this multicenter prospective study, lower SPIO doses (undiluted 1.5 vs. 1.0 mL) in different timeframes (perioperative vs. 1–7 days preoperative) and injection sites (subareolar vs. peritumoral) were compared to the previous standard (diluted 2.0 mL perioperatively) from the earlier Nordic trial. RI + BD were co-administered as background. In total, 534 patients were analyzed. SPIO SLN detection rates were similar (97.5% vs. 100% vs. 97.6%, p = 0.11) and respectively non-inferior to the dual technique. Significantly more SLNs were retrieved in the preoperative 1.0 mL cohort compared with 1.5 mL and the Nordic cohorts (2.18 vs. 1.85 vs. 1.83, p = 0.003). Thus, SPIO at 1.5 and 1.0 mL was non-inferior to both Sienna+® and the dual technique for SLN detection.Superparamagnetic iron oxide nanoparticles (SPIO) are non-inferior to radioisotope and blue dye (RI + BD) for sentinel lymph node (SLN) detection. Previously, 2 mL SPIO (Sienna+®) in 3 mL NaCl was used. In this dose-optimizing study, lower doses of a new refined SPIO solution (Magtrace®) (1.5 vs. 1.0 mL) were tested in different timeframes (0–24 h perioperative vs. 1–7 days preoperative) and injections sites (subareolar vs. peritumoral). Two consecutive breast cancer cohorts (n = 328) scheduled for SLN-biopsy were included from 2017 to 2019. All patients received isotope ± blue dye as back-up. SLNs were identified primarily with the SentiMag® probe and thereafter a gamma-probe. The primary endpoint was SLN detection rate with SPIO. Analyses were performed as a one-step individual patient-level meta-analysis using patient-level data from the previously published Nordic Trial (n = 206) as a third, reference cohort. In 534 patients, the SPIO SLN detection rates were similar (97.5% vs. 100% vs. 97.6%, p = 0.11) and non-inferior to the dual technique. Significantly more SLNs were retrieved in the preoperative 1.0 mL cohort compared with 1.5 and the 2.0 mL cohorts (2.18 vs. 1.85 vs. 1.83, p = 0.003). Lower SPIO volumes injected up to 7 days before the operation have comparable efficacy to standard SPIO dose and RI + BD for SLN detection.

An easy and low-cost biomagnetic methodology to study regional gastrointestinal transit in rats.

The identification of gastrointestinal (GI) motility disorders requires the evaluation of regional GI transit, and the development of alternative methodologies in animals has a significant impact on translational approaches. Therefore, the purpose of this study was to validate an easy and low-cost methodology (alternate current biosusceptometry- ACB) for the assessment of regional GI transit in rats through images. Rats were fed a test meal containing magnetic tracer and phenol red, and GI segments (stomach, proximal, medial and distal small intestine, and cecum) were collected to assess tracer's retention at distinct times after ingestion (0, 60, 120, 240, and 360min). Images were obtained by scanning the segments, and phenol red concentration was determined by the sample's absorbance. The temporal retention profile, geometric center, gastric emptying, and cecum arrival were evaluated. The correlation coefficient between methods was 0.802, and the temporal retention of each segment was successfully assessed. GI parameters yielded comparable results between methods, and ACB images presented advantages as the possibility to visualize intrasegmental tracer distribution and the automated scan of the segments. The imaging approach provided a reliable assessment of several parameters simultaneously and may serve as an accurate and sensitive approach for regional GI research in rats.

An exploratory study on the use of different composite magnetic and colour fingerprints in aeolian sediment provenance fingerprinting

There is an urgent need for reliable and cost-effective sediment source tracing techniques for apportioning aeolian sediment (sand dune) sources for guiding the selection of best management practices for wind erosion control. Accordingly, the main aim of this study was to quantify the contributions of aeolian sources to sand dune target sediment samples collected in a case study in central Iran using a source fingerprinting procedure based on low-cost fingerprints comprising colour and magnetic tracers. Colour (RGB), magnetic susceptibility (χlf and χhf) and 13 colour and magnetic indices were measured on 54 aeolian sediment source samples and ten aeolian target sediment samples. Three different composite fingerprints for discriminating and apportioning the aeolian sediment sources were selected based on a combination of statistical tests comprising the Kruskal–Wallis H test (KW-H), discriminant function analysis (DFA), principal component & classification analysis (PCCA), and a general classification & regression tree (GC&RT) model. The Modified MixSIR Bayesian un-mixing model was used to apportion aeolian source contributions using the final composite fingerprints. The composite signatures all suggested that the salt flat plain was the dominant (average 63%, and standard deviation, SD, 5.9%) source of the aeolian target sediment samples, whilst agricultural land was second (average 63%, SD 5.6%,) most important. The root mean square difference between the apportionment results based on the three composite fingerprints ranged from 0.2% to 8.3%. Pairwise comparisons of the posterior distributions for the predicted source proportions generated using the three composite signatures showed that eight of 12 pairwise comparisons were not significantly different. Virtual mixture accuracy tests of the fingerprinting models using the three composite signatures suggested errors ranging between 2.2%−20.6% (with a mean of 9.9%), 1.4%−17.0% (mean value 8.3%), and 0.03%−1.0% (mean value 0.8%). The results support the use of low-cost colour and magnetic tracers by investigations into aeolian sediment provenance.

Non-radioactive and sensitive tracking of neutrophils towards inflammation using antibody functionalized magnetic particle imaging tracers.

White blood cells (WBCs) are a key component of the mammalian immune system and play an essential role in surveillance, defense, and adaptation against foreign pathogens. Apart from their roles in the active combat of infection and the development of adaptive immunity, immune cells are also involved in tumor development and metastasis. Antibody-based therapeutics have been developed to regulate (i.e. selectively activate or inhibit immune function) and harness immune cells to fight malignancy. Alternatively, non-invasive tracking of WBC distribution can diagnose inflammation, infection, fevers of unknown origin (FUOs), and cancer. Magnetic Particle Imaging (MPI) is a non-invasive, non-radioactive, and sensitive medical imaging technique that uses safe superparamagnetic iron oxide nanoparticles (SPIOs) as tracers. MPI has previously been shown to track therapeutic stem cells for over 87 days with a ~200 cell detection limit. In the current work, we utilized antibody-conjugated SPIOs specific to neutrophils for in situ labeling, and non-invasive and radiation-free tracking of these inflammatory cells to sites of infection and inflammation in an in vivo murine model of lipopolysaccharide-induced myositis. MPI showed sensitive detection of inflammation with a contrast-to-noise ratio of ~8-13.

Engineering of magnetic nanoparticles as magnetic particle imaging tracers.

Magnetic particle imaging (MPI) has recently emerged as a promising non-invasive imaging technique because of its signal linearly propotional to the tracer mass, ability to generate positive contrast, low tissue background, unlimited tissue penetration depth, and lack of ionizing radiation. The sensitivity and resolution of MPI are highly dependent on the properties of magnetic nanoparticles (MNPs), and extensive research efforts have been focused on the design and synthesis of tracers. This review examines parameters that dictate the performance of MNPs, including size, shape, composition, surface property, crystallinity, the surrounding environment, and aggregation state to provide guidance for engineering MPI tracers with better performance. Finally, we discuss applications of MPI imaging and its challenges and perspectives in clinical translation.