Magnetic Nanotherapy Research Articles

Biosynthesized iron oxide nanoparticles: Cytotoxic evaluation against human colorectal cancer cell lines

Recent research reveals the impending importance of nanoparticles in medicine. Among various deadly diseases, cancer is one of the most dangerous categories for which no treatment is currently available other than surgery, chemotherapy, radiotherapy, etc. One major issue with these treatments is the side effect such as fatigue, pain, hair loss, vomiting, etc. So a better alternative is extremely desirable in these highly demanding times. Iron oxide nanoparticles (NPs) have attracted wide interest due to their superparamagnetic properties, nontoxicity and have been used in many fields. Iron oxide NPs are used in cancer magnetic nano therapy because of their magneto-spin effects in free radical reactions and ability to produce oxygen radicals. Iron oxide NPs may also be used in magnetic hyperthermia as a cancer treatment method. In the present study, we studied the apoptosis-inducing activity of biosynthesized iron oxide NPs synthesized via green method using Pimenta dioica leaf extract. The extent of cell viability was investigated using MTT assay. It was observed that the iron oxide NPs significantly reduce the cell viability in a concentration-dependent manner. The studies confirm that the biosynthesized iron oxide NPs have an efficient growth-inhibitory effect on DLD-1 human colon cancer cells and are less toxic on normal L929 (fibroblast) cells.

The comparison between superparamagnetic and ferromagnetic iron oxide nanoparticles for cancer nanotherapy in the magnetic resonance system

The paper aims to compare zeta potentials, magnetic properties, electron spin resonance, photoluminescence (PL) spectra and antitumor effect of magneto-mechano-chemically synthesized magneto-sensitive nanocomplexes loaded with the anticancer drug doxorubicin (DOXO) during nanotherapy of Walker-256 carcinosarcoma carried out by a magnetic resonance system. Diamagnetic DOXO acquired the properties of a paramagnetic substance after synthesis. MNC comprising superparamagnetic nanoparticles (NP) and DOXO had different g-factors, zeta potentials, a lower saturation magnetic moment, area of the hysteresis loop, and a higher coercivity compared to similar MNC with ferromagnetic NP. The main PL peak of MNC spectrum was defined by DOXO at 598 nm. MNC composed of superparamagnetic NP and DOXO showed a lower standard deviation of the normal PL spectral distribution than MNC based on ferromagnetic NP in relation to conventional DOXO. MNC containing superparamagnetic NP responded to resonance conditions leading to a more pronounced antitumor effect compared to MNC with ferromagnetic NP in the course of magnetic nanotherapy for Walker-256 carcinosarcoma bearing animals (temperature inside the tumor did not exceed 40 °C). Therefore, these findings are associated with differences in chemotherapeutic effect between MNC due to a different surface charge and conformational changes in DOXO molecules during its magnetoelectric interaction with single- and multidomain NP.

Antitumor effect of superparamagnetic iron oxide nanoparticles conjugated with doxorubicin during magnetic nanotherapy of Lewis Lung carcinoma

Superparamagnetic Iron(II,III) oxide Fe3O4 nanoparticles (NP) in magnetic nanocomplex (MNC2) with antitumor drug doxorubicin (DOXO) induced a greater antitumor and antimetastatic effect compared to the magnetic nanocomplex (MNC1) composed of ferromagnetic nanoparticles with DOXO and conventional DOXO during magnetic nanotherapy of animals with Lewis lung carcinoma. The intratumor temperature did not exceed 39 °C. MNC2 had g‐factors of 2.00, 2.30 and 4.00. MNC1 had the g‐factor of 2.50. The MNC2 increased the content of Nitric oxide – Iron(II) sulfide protein N complex and NADH‐ubiquinone in tumor as compared to MNC1 or conventional DOXO. The results have been analyzed in terms of increased level of reactive oxygen species which could induce death of tumor cells.

Магнітна нанотерапія тварин із карциносаркомою Уокер-256

Магнітна нанотерапія тварин із карциносаркомою Уокер-256

Imaging of Guerin Carcinoma During Magnetic Nanotherapy

We conducted a detailed imaging study of transplanted Guerin’s carcinoma in rats during treatment with magnetic nanotherapy. We show that treatment with magneto-mechano-chemically synthesized magnetic nanocomplexes based on Fe3O4 nanoparticles conjugated with the antitumor agent doxorubicin and followed by irradiation with local electromagnetic irradiation resulted in a better outcome than treatment with conventional doxorubicin or treatment with magnetic nanocomplexes without electromagnetic irradiation. Analysis of magnetic resonance images obtained over time showed that the application of local electromagnetic irradiation did not alter the position of magnetic nanocomplexes in the tumor. B-mode sonography demonstrated that injection of magnetic nanocomplexes into the tumor and subsequent electromagnetic irradiation resulted in increased echogenicity throughout the tumor, which is characteristic of increased blood perfusion. Color Doppler ultrasound imaging showed that the largest decrease of tumor vessel area occurred after injection of magnetic nanocomplex and application of local electromagnetic irradiation. Elastography performed on a diagnostic ultrasound system showed that the injection of magnetic nanocomplexes in tumors increased Young’s modulus for Guerin’s carcinoma compared to tumors not treated with nanoparticles. These results suggest that a theranostics approach-combination MRI together with ultrasound elastography during treatment of tumor may provide a useful method to monitor the effectiveness treatment of cancer patients during magnetic nanotherapy.

Analysis of ultrasound elastography images for tumor guerin carcinoma during magnetic nanotherapy

Analysis of ultrasound elastography images for tumor guerin carcinoma during magnetic nanotherapy