Fe3O4 Magnetic Nanoparticles Research Articles

Olive leaf extract-assisted preparation of nanoferrite for adsorptive removal of cationic dye

In this work, magnetic Fe3O4 nanoparticles synthesized with olive leaf extract were used to investigate the adsorption of Basic violet 16 from aqueous solutions. The physicochemical characteristics of Fe3O4 nanoparticles were evaluated by DLS, zeta potential, XRD, FTIR SEM, EDX, N2 adsorption-desorption, TEM, and VSM analyses. Using DLS analysis, the average size of the Fe3O4 nanoparticles was found to be 231.2 nm. Zeta potential studies indicated that the synthesized nanoparticles were moderately stable, and zeta potential was calculated as −14.9 mV. According to TEM analysis, the synthesized nanoparticles ranged in size from 6.28 to 17.3 nm. The optimum parameters for the adsorption of basic violet 16 with Fe3O4 nanoparticles were determined as pH: 9.0, adsorbent dose: 0.8 g, contact time: 210 min, stirring speed: 250 rpm. Langmuir, Freundlich, Dubinin Radushkevich (D-R) and Temkin isotherm models were used to characterize the basic violet 16 sorption data onto Fe3O4 nanoparticles and it was seen that Basic violet 16 adsorption onto the Fe3O4 nanoparticles followed Langmuir isotherm model at all temperatures. The maximum adsorption capacity of Basic violet 16 with Fe3O4 nanoparticles was determined as 117.64 mg/g at 318 K. Pseudo-first-order, pseudo-second-order and intraparticle diffusion models were used to describe the adsorption kinetics at 298–318 K. The adsorption followed the pseudo-second-order kinetic model. Adsorption of Basic violet 16 onto Fe3O4 nanoparticles occurred spontaneously and endothermically. Characteristic peaks Fe3O4 nanoparticles were visible in the XRD analysis. The SBET surface area dropped from 110.42 m2/g to 91.27 m2/g after dye adsorption. Peaks in the FTIR analysis showed that Basic violet 16 had been successfully adsorbed on Fe3O4 nanoparticles.

Harnessing chemical functionality of xylan hemicellulose towards carbohydrate polymer-based pH/magnetic dual-responsive nanocomposite hydrogel for drug delivery

This study reports a pH/magnetic dual-responsive hemicellulose-based nanocomposite hydrogel with nearly 100 % carbohydrate polymer-based and biodegradable polymer compositions for drug delivery. We synthesized pure Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) using a co-precipitation method, then engineering xylan hemicellulose (XH), acrylic acid, poly(ethylene glycol) diacrylate, and Fe3O4 to synthesize the pH/magnetic dual-responsive hydrogel (Fe3O4@XH-Gel), through graft polymerization on XH with in-situ doping Fe3O4 MNPs initiated by the ammonium persulfate/tetramethylethylenediamine redox system. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), X-ray diffractometry (XRD), scanning electron microscopy and energy dispersive spectrometer (SEM-EDS), high-resolution transmission electron microscopy (HRTEM), Brunauer-Emmett-Teller (BET), swelling gravimetric analysis, vibrating sample magnetometer (VSM) were employed to analyze the hydrogel's chemical structures, morphologies, pH-responsive behaviors, and magnetic responsiveness characteristics, mechanical and rheological properties, as well as cytotoxicity and biodegradability. The results indicate that the Fe3O4@XH-Gel exhibited excellent dual responsiveness to pH and magnetism. Furthermore, an emphasis was placed on the in-depth analysis of the pH response mechanism. Finally, we utilized this cutting-edge hydrogel to investigate the controlled-release behavior of two model drugs, Acetylsalicylic acid and Theophylline. The hydrogel demonstrated exceptional controlled release attributes, positioning it as a potential carrier for targeted drug delivery, particularly to the gastrointestinal conditions.

Adsorption‐Based Approaches for Exploring Nanoparticle Effectiveness in Wastewater Treatment

AbstractThe treatment of wastewater is essential to preserving the environment and public health. Recent advances in this field have made nanoparticles attractive tools due to their exceptional selectivity, reactivity, and surface area. Particularly, their use in adsorption‐based wastewater treatment has garnered significant interest, focusing on how well nanoparticles can adsorb chemical contaminants, thereby improving treatment effectiveness. A thorough analysis explores various aspects of nanoparticles in wastewater treatment, including different types of nanoparticles used for adsorption, underlying mechanisms, and numerous factors affecting their pollutant removal efficiency. However, it also recognizes potential negative environmental impacts, underscoring the need for comprehensive risk assessments, including eco‐toxicity, release dynamics, and long‐term ecological effects. In light of these challenges, the review emphasizes the revolutionary potential of new nanoparticles in transforming wastewater treatment paradigms. Examples include ZnO nanoparticles and Fe₃O₄ magnetic nanoparticles (MNPs), which excel at removing dyes and reducing sludge volume, while CuO and TiO₂ nanoparticles rapidly eliminate organic pollutants and heavy metals like Pb2+. Additionally, graphical illustrations highlight nanoparticles′ advantages, especially in removing cadmium. These findings underscore the future direction of research on wastewater treatment using nanoparticles, pointing toward increased sustainability and efficiency in addressing water pollution issues.

Immobilization of doxorubicin on Fe3O4 magnetic nanoparticles modified with iron and silicon glycerolates

Immobilization of doxorubicin on Fe3O4 magnetic nanoparticles modified with iron and silicon glycerolates

Investigating the catalytic performance of polyoxometalate immobilized on magnetic chitosan in the oxidative desulfurization process

In this study, two types of polyoxometalate, one of Keggin type (PMo6W6O40) and the other of Dawson type (H11P2W13V5O62), were synthesized and fixed on chitosan with magnetic properties. The performance of synthesized catalysts was evaluated on the efficiency of removing aromatic sulfur compounds from the fuel model by the oxidative desulfurization method. Factors affecting the oxidation of sulfur compounds, such as reaction temperature, catalyst dosage, reaction time, and the ratio of oxidant to sulfur, were investigated to find the optimal conditions. The presence of Fe3O4 magnetic nanoparticles in the catalyst leads to the ease of separation from the petroleum model. The magnet-separated catalyst could be recycled for 5 catalytic cycles without significantly reducing removal efficiency.

A new SERS quantitative analysis strategy for ultratrace chloramphenicol with Fe3O4@MIP nanocatalytic probe

Three functional magnetic nanocatalytic probe, which integrates recognition, catalytic amplification, and separation enrichment, is a new approach to construct a simple, fast, highly selective, and sensitive analytical method. In this article, a new magnetic nanosurface molecularly imprinted polymer nanoprobe (Fe3O4@MIP) with trifunctionality was rapidly prepared using a microwave-assisted method with magnetic Fe3O4 nanoparticles as a substrate, chloramphenicol (CAP) as a template molecule, and methacrylic acid as a functional monomer. The characterized nanoprobe was found that could specifically recognize CAP, strongly catalyze the new indicator nanoreaction of fructose (DF)-HAuCl4. The gold nanoparticles (AuNPs) exhibit strong resonance Rayleigh scattering (RRS) and surface enhanced Raman scattering (SERS) effects. Upon addition of CAP, the SERS/RRS signals were linearly weakened. Accordingly, a new SERS/RRS analysis platform for highly sensitive and selective determination of CAP was constructed. The SERS linear range was 0.0125–0.1 nmol/L, with detection limit (DL) of 0.004 nmol/L CAP. Furthermore, it could be combined with magnet-enriched separation to further improve the sensitivity, with a DL of 0.04 pmol/L CAP. The SERS method has been used for the determination of CAP in real samples, with relative standard deviations of 2.37–9.89 % and the recovery of 95.24–107.1 %.

A Versatile C/Fe3O4/C Self‐Heating Electrode for Universal Application of Alternating Magnetic Fields in Electrocatalytic Hydrogen Production

AbstractThe high‐frequency alternating magnetic field (AMF) is considered as a fascinating heating treatment that provides a noninvasive solution to enhance the catalytic efficiency of electrocatalyst. However, practical applications of AMF in electrochemistry are primarily concentrated on magnetic mediums. To broaden its application into nonmagnetic catalysts, herein, a practicable method is reported by modifying the working electrode substrate with magnetic Fe3O4 nanoparticles (NPs), which serves as both substrate and self‐heating medium by virtue of its rapid and efficient magnetic heating effect associated with Néel relaxation triggered by external high‐frequency AMF, thus boosting the catalyst performance upon it. To verify it, Pt NPs and Pt single atoms (SAs), as two representative non‐magnetic catalyst, are selected for trials. The results, reveal that, when AMF is applied, Pt NPs@C/Fe3O4/C and Pt SAs@C/Fe3O4/C display remarkable enhancement of hydrogen evolution reaction magnetocurrent density respectively by ≈ 146% and ≈185%, whereas on unmodified bare glassy carbon there both show unnoticeable change in catalytic performance. The developed strategy opens up a vast space for exploiting the energy of a weak, non‐invasive magnetic field.

Radiation-Induced Synthesis and Superparamagnetic Properties of Ferrite Fe3O4 Nanoparticles.

Ultra-small magnetic Fe3O4 nanoparticles are successfully synthesized in basic solutions by using the radiolytic method of the partial reduction in FeIII in the presence of poly-acrylate (PA), or by using the coprecipitation method of FeIII and FeII salts in the presence of PA. The optical, structural, and magnetic properties of the nanoparticles were examined using UV-Vis absorption spectroscopy, high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and SQUID magnetization measurements. The HRTEM and XRD analysis confirmed the formation of ultra-small magnetite nanoparticles in a spinel structure, with a smaller size for radiation-induced particles coated by PA (5.2 nm) than for coprecipitated PA-coated nanoparticles (11 nm). From magnetization measurements, it is shown that the nanoparticles are superparamagnetic at room temperature. The magnetization saturation value Ms = 50.1 A m2 kg-1 of radiation-induced nanoparticles at 60 kGy is higher than Ms = 18.2 A m2 kg-1 for coprecipitated nanoparticles. Both values are compared with nanoparticles coated with other stabilizers in the literature.

Initial therapeutic evidence of a borosilicate bioactive glass (BSG) and Fe3O4 magnetic nanoparticle scaffold on implant-associated Staphylococcal aureus bone infection

Implant-associated Staphylococcus aureus (S. aureus) osteomyelitis is a severe challenge in orthopedics. While antibiotic-loaded bone cement is a standardized therapeutic approach for S. aureus osteomyelitis, it falls short in eradicating Staphylococcus abscess communities (SACs) and bacteria within osteocyte-lacuna canalicular network (OLCN) and repairing bone defects. To address limitations, we developed a borosilicate bioactive glass (BSG) combined with ferroferric oxide (Fe3O4) magnetic scaffold to enhance antibacterial efficacy and bone repair capabilities. We conducted comprehensive assessments of the osteoinductive, immunomodulatory, antibacterial properties, and thermal response of this scaffold, with or without an alternating magnetic field (AMF). Utilizing a well-established implant-related S. aureus tibial infection rabbit model, we evaluated its antibacterial performance in vivo. RNA transcriptome sequencing demonstrated that BSG + 5%Fe3O4 enhanced the immune response to bacteria and promoted osteogenic differentiation and mineralization of MSCs. Notably, BSG + 5%Fe3O4 upregulated gene expression of NOD-like receptor and TNF pathway in MSCs, alongside increased the expression of osteogenic factors (RUNX2, ALP and OCN) in vitro. Flow cytometry on macrophage exhibited a polarization effect towards M2, accompanied by upregulation of anti-inflammatory genes (TGF-β1 and IL-1Ra) and downregulation of pro-inflammatory genes (IL-6 and IL-1β) among macrophages. In vivo CT imaging revealed the absence of osteolysis and periosteal response in rabbits treated with BSG + 5%Fe3O4 + AMF at 42 days. Histological analysis indicated complete controls of SACs and bacteria within OLCN by day 42, along with new bone formation, signifying effective control of S. aureus osteomyelitis. Further investigations will focus on the in vivo biosafety and biological mechanism of this scaffold within infectious microenvironment.

Directional surface plasmon polariton scattering using single magnetic nanoparticles.

Directional surface plasmon polaritons (SPPs) are expected to promote the energy efficiency of plasmonic devices, via limiting the energy in a given spatial domain. The directional scattering of dielectric nanoparticles induced by the interference between electric and magnetic responses presents a potential candidate for directional SPPs. Magnetic nanoparticles can introduce permeability as an extra manipulation, whose directional scattered SPPs have not been investigated yet. In this work, we demonstrated the directional scattered SPPs by using single magnetic nanoparticles via simulation and experiment. By increasing the permeability and particle size, the high-order TEM modes are excited inside the particle and induce more forward directional SPPs. It indicated that the particle size manifests larger tuning range compared with the permeability. Experimentally, the maximum forward-to-backward (F-to-B) SPP scattering intensity ratio of 118.52:1 is visualized by using a single 1 μm Fe3O4 magnetic nanoparticle. The directional scattered SPPs of magnetic nanoparticles are hopeful to improve the efficiency of plasmonic devices and pave the way for plasmonic circuits on-chip.

Poorly-/well-dispersed Fe3O4: Abnormal influence on electromagnetic wave absorption behavior of high-mechanical performance polyurea

In this work, novel core–shell Fe3O4 nanoparticles were prepared by self- assembly technology using phytate-melamine aggregates (PM) or phytate-melamine-Fe3+ aggregates (PM-Fe) as building block. A polyurea (PUA) matrix was filled with magnetic nanoparticle. The influence of Fe3O4, Fe3O4@PM or Fe3O4@PM-Fe content and dispersion on the mechanical and electromagnetic wave (EMW) absorption properties of PUA composite were investigated. Good dispersion of modified Fe3O4 in PUA matrix was obtained. Loading well-dispersed Fe3O4 significantly enhanced the mechanical properties of their PUA nanocomposite under quasi-static tensile, nanoindentation, and dynamic compression tests. Excellent EMW absorption performance of PUA/Fe3O4@PM nanocomposite was desired. Although well-dispersed Fe3O4@PM could effectively enhance the mechanical properties, nonetheless it surprisingly plays a negative role in the EMW absorption performance. The Fe3O4@PM with high dispersion and interfacial compatibility in the PUA matrix was detrimental to EMW absorption performance, which contradicts the well accepted EMW absorption mechanisms. A novel mechanism and model were suggested, along with a fresh understanding of the role of Fe3O4 in the EMW absorption of PUA composite. Furthermore, the Fe3O4@PM-Fe, obtained by constructing a conductive network on the surface of Fe3O4@PM, was used to investigate this assumption. The radar cross section simulation also demonstrated the superior EMW attenuation capability of PUA nanocomposites in the service condition. This work is beneficial to the development of EMW absorber, not limited to Fe3O4 magnetic nanoparticles.

Preparation of the Porous Copper Foil Current Collector for High-Performance Lithium-Ion Batteries by Electro-Codeposition: Effect of Stirring Speed

As an indispensable component of lithium-ion batteries (LIBs), the application of porous copper (Cu) foil current collector is an effective method to improve the performance and energy density of LIBs. In this work, a porous Cu (PCu) foil was fabricated by acid washing the Cu-Fe3O4 foil prepared by the electro-codeposition in a stirred CuSO4 solution containing the magnetic Fe3O4 nanoparticles. The effect of the stirring speed on the properties of PCu foils and the battery performance of PCu foil as current collectors were investigated. The surface roughness of the PCu foil increases, while its volume density decreases as the stirring speed rises. The volume density of PCu foil (6.63 g cm−3) prepared at 400 rpm (PCu400) is reduced by 22.4% than that of the commercial Cu foil (8.55 g cm−3). The specific capacity (241.2 mAh g−1) of the cell with PCu400 current collector is 31.3% higher than that of the cell with commercial Cu current collector (183.7 mAh g−1) after 300 charge-discharge cycles at 2 C. The electro-codeposition of porous Cu foil current collectors offers a promising method for high-performance lightweight LIBs.

Microstructure and magnetic properties of the FeSiBCuNb nanocrystalline soft magnetic composites coated with oil-phase single Fe3O4 nanoparticles by thermal decomposition method

Here, the core-shell structured FeSiBCuNb/Fe3O4 soft magnetic composites (SMCs) were successfully designed and fabricated by coating Fe3O4 magnetic nanoparticles on the surface of phosphorylated FeSiBCuNb powder. The effects of Fe3O4 and Polyvinylpyrrolidone (PVP) contents on the micromorphology, interface structure, and soft magnetic properties of SMCs were systematically investigated. The results demonstrate that the Fe3O4 magnetic nanoparticles are uniformly distributed on the surface of magnetic powders, which fill up the air gap in-between the magnetic powder and increase the resistivity. It shows that the permeability gradually decreases with the increase of Fe3O4 from 0 wt% to 1 wt%, while the loss decreases first and then increases. The loss separation of SMCs indicates that the Fe3O4 nanoparticles effectively reduce the hysteresis loss and eddy current loss. Furthermore, the FeSiBCuNb/Fe3O4 SMCs with the addition of PVP as a binder enhance the binding force between Fe3O4 nanoparticles and the powder matrix, which facilitates the formation of a uniform and dense interface structure. When adding 0.3 wt% PVP + Fe3O4 nanoparticles, the FeSiBCuNb/Fe3O4 SMCs exhibit excellent soft magnetic properties with ultra-low loss of 138.5 kW/m3 (100 mT/50 kHz), high permeability of 54, and good frequency stability. The presented results provide a new strategy for synthesizing ultra-low core loss and frequency stability of core-shell structured SMCs, which has great potential for applications at high frequencies.

A comprehensive study on RSM optimization of the influencing variables on loading/releasing of ciprofloxacin onto/from magnetic iron nanoparticles

Fe3O4 MNPs were synthesized by the co-precipitation method and characterized via SEM, EDX, FTIR, XRD, VSM, TGA, and x-ray map. Its pHpzc was obtained at 5.4. The loading and releasing conditions for Ciprofloxacin (CIP) on/from Fe3O4 magnetic nanoparticles (MNPs) were optimized via response surface methodology (RSM). We found that pH was the most influential factor in the loading and releasing processes. The center point conditions for the loading were pH 7, CIP concentration of 14 ppm, adsorbent dose of 11 mg, and shaking time of 9 h, while for the release process, were pH 5.1, sample dose of 11 mg, and releasing time of 5.1 h. The optimal RSM run conditions for loading were pH: 5, CCIP: 13 ppm, dose: 7 mg, time: 20 h, and for the releasing process were pH: 2.7, dose: 11 mg, time: 7 h.

Hydroxide-Derived Nanostructures: Scalable Synthesis, Characterization, Properties, and Potential Applications.

Metal oxide nanostructures have received an increasing attention owing to their unique chemical and physical properties along with their widespread applications in various fields. This article provides an overview of the recent discovery - christened Hydroxides-Derived Nanostructures, or HDNs - in which hydroxide aqueous solutions (mostly tetramethylammonium hydroxide, TMAH) are reacted at temperatures < 100°C and under atmospheric pressure with various metal-containing precursors to scalably prepare novel metal oxide nanostructures. In one case, a dozen commercial and earth abundant Ti-containing powders such as binary carbides, nitrides, borides, among others, are converted into new, 1D TiO2-based lepidocrocite (1DL) nanofilaments (NFs). Application-wise, the 1DLs show outstanding performance in a number of energy, environmental, and biomedical fields such as photo- and electrocatalysis, water splitting, lithium-sulfur and lithium-ion batteries, water purification, dye degradation, cancer therapy, and polymer composites. In addition to 1DL, the HDNs family encompasses other metal oxides nanostructures including magnetic Fe3O4 nanoparticles and MnO2 birnessite-based crystalline 2D flakes. The latter showed promise in electrochemical energy conversion and storage applications. The developed recipe provides a new vista in the molecular self-assembly synthesis of nanomaterials that can advance the field with a library of novel nanostructures with substantial implications in a multitude of fields.

Inhibition of EGFR1 in Triple Negative Breast Cancer Cells Using siRNA Loaded with Fe3O4 Magnetic Nanoparticles

Inhibition of EGFR1 in Triple Negative Breast Cancer Cells Using siRNA Loaded with Fe3O4 Magnetic Nanoparticles

Fabrication, characterization, spectroscopic, and magnetic properties of polyaniline/magnetite (PANi/Fe3O4) nanocomposites

In this research, pure polyaniline (PANi), pure magnetite (Fe3O4), and then PANi/ Fe3O4 nanocomposites were prepared and characterized. FT-IR spectra of the prepared samples indicate successful polymerization of PANi onto Fe3O4. PANi/Fe3O4 nanocomposites exhibit broad absorption bands at specific wavenumbers, indicating the presence of various functional groups. The observed blue shift in certain peaks suggests the interaction between nitrogen atoms and oxygen atoms on the magnetite surface through hydrogen bonding. The intensity and wavenumber of the Fe3O4 band decrease, indicating weakened Fe–O bonds. These findings demonstrate a strong interaction and combination between PANi and Fe3O4 in the nanocomposites. Fe3O4 nanoparticles exhibit a regular spherical shape with an average diameter of 16 nm. However, they also show non-uniform size distribution due to aggregation into larger secondary particles, likely driven by attractive forces. PANi/Fe3O4 core-shell nanocomposites demonstrate clear morphological changes, with Fe3O4 nanoparticles well spread and wrapped by the PANi shell. The electron diffraction technique confirms the crystalline structure of Fe3O4 nanoparticles, showing diffraction rings consistent with magnetite. The results confirm the potential of incorporating magnetic Fe3O4 nanoparticles to impart magnetic functionality in the resulting PANi-based nanocomposite materials. Magnetic saturation measurements displayed an enhancement in magnetization with increasing Fe3O4 nanoparticles. The change in magnetic behavior for doped samples provides a means of tuning the magnetization strength of the composites to meet the application requirement such as data storage and magnetic sensors.

Preparation and Characterization of C@Fe3O4 Supported Pd Magnetic Nanoparticles for Degradation of Dye Wastewater

The core-shell structure Fe3O4@C magnetic nanoparticles were synthesized with superparamagnetic Fe3O4 nanosphere as a magnetic core, and soluble starch resin as a carbon source via a solvothermal method. Silica-iron oxide and Fe3O4@C carriers with a core-shell structure were prepared by carbonization of organic material on the surface of Fe3O4 nanoparticles, Fe3O4@C supported Pd were processed into magnetic nano-catalysts with core-shell structure, and their catalytic properties were investigated. The resulting environmentally friendly magnetic material can be used to degrade dye wastewater. The structure of magnetic nanoparticles was characterized using TEM, XRD and VSM. The effects of preparation conditions in the structure of the Fe3O4@C magnetic nanoparticles were taken out. The results indicate that from XRD, the magnetic nano particles Fe3O4@C synthesized of carbon sources have amorphous carbon diffraction peak except for all the characteristic peaks of Fe3O4. The saturation magnetization Fe3O4, Fe3O4@SiO2 and Fe3O4@C – 59.14 emu/g, 49.12 emu/g and 27.95 emu/g, respectively.

Green preparation of Fe3O4/HA/CS magnetic nanoparticles for oil–water separation

AbstractBACKGROUNDDemulsification using chitosan (CS)‐coated magnetic nanomaterials has garnered significant attention. However, traditional methods for synthesizing chitosan (CS)‐coated magnetic nanomaterials are often contaminated, laborious, and time‐consuming. Moreover, they showed poor demulsification efficiency due to the occupation of free amino groups in CS by crosslinking agents.RESULTSCS‐coated Fe3O4 magnetic nanoparticles (MNPs) were synthesized using humic acid (HA) as a bridge. The Fe3O4/HA/CS MNPs are recyclable demulsifiers for hexadecane‐water microemulsions. The impacts of MNP dosage, separation time, and pH on demulsification efficiency were studied comprehensively. Compared with Fe3O4/HA and Fe3O4/CS MNPs, the demulsification efficiency of the Fe3O4/HA/CS MNPs was superior. The pseudo‐second‐order kinetic model and the Sips isotherm best describe the demulsification process. Combined with the influence factors of demulsification efficiency, the demulsification mechanism involves electrostatic interactions between MNPs and oil microparticles. The maximum adsorption capacity for oil by the Fe3O4/HA/CS MNPs was 91.73 mg g−1.CONCLUSIONThis research presents a new method for connecting CS to MNPs with a high surface positive charge. As synthesized Fe3O4/HA/CS MNPs is expected to serve as highly efficient and recyclable demulsifiers for emulsified oil wastewater. © 2024 Society of Chemical Industry (SCI).

Nerve tissue regeneration based on magnetic and conductive bifunctional hydrogel scaffold

The growth of neurons is often influenced by magnetic and electrical physical factors, and there is more and more research focused on designing a magnetic and conductive engineering scaffolds for nerve injury repair. In this study, electrospun poly lactic-co-glycolic acid (PLGA) fibers containing Fe3O4 magnetic nanoparticles were cryo-cut into magnetic short fibers (MSFs) and subsequently incorporated into sodium alginate (SA)/carboxymethyl chitosan (CMCS) hydrogel along with the addition of multi-wall carbon nanotubes (MWCNTs). MSFs can also achieve alignment by external magnetic fields within the hydrogel to construct magnetic and conductive bifunctional MSF/MWCNT/SA/CMCS hydrogel with anisotropic structure. The magnetic-conductive bifunctional hydrogel exhibited good magnetic responsiveness, electrically activity and mechanical property, in which both conductivity and mechanical property increased with the enhancement of MWCNTs contents. Notably, appearing of aligned structure significantly promoted the mechanical property of MSF/SA/CMCS hydrogel. The MSF/SA/CMCS hydrogel with aligned structure also facilitated greater primary neuron cells proliferation than random ones. Compared with individual magnetic or conductive hydrogel, there was a significant increase in cell viability in the primary cultured cortical neurons on MSF/MWCNT/SA/CMCS hydrogel with aligned structure. Simultaneously, electrical stimulation (ES) combined with magnetic stimulation (MS) delivered via magnetic and conductive bifunctional MSF/MWCNT/SA/CMCS hydrogel with aligned structure produced a better effect on promoting neuronal growth compared with individual ES or MS. Besides, hot plate measurement indicated that MSF/MWCNT/SA/CMCS hydrogel with aligned structure and MS significantly decreased the mice licking latency after sciatic nerve injury. Animal behavior studies confirmed the protective role of synergistic MS with magnetic hydrogel. Such hydrogel scaffolds have long-term potential for nerve tissue regeneration.