Under ultrasound irradiation, a highly selective Magnetic Molecularly Imprinted Polymer (MMIP) was synthesized by polymerizing monomers on magnetite. The Density Functional Theory was utilized for the selection of suitable monomers based on binding energy interaction between quercetin and monomers (Acrylamide, Acrylonitrile, Methacrylic Acid). Quercetin served as the template molecule, while acrylamide was used as the functional monomer, and a mixture of DMSO and water was employed as the porogen solvent. This study aims to synthesize and evaluate the effectiveness of MMIP and analyze its adsorption isotherm and kinetics. It was observed that the Freundlich model supports the adsorption of quercetin on MMIP, and the kinetic data correlates better with the Pseudo-first-order kinetic model. The MMIP exhibited a pronounced selectivity in the adsorption of quercetin with a maximum adsorption capacity of 10.07 mg g-1 in 30 min. Furthermore, the physiochemical properties of synthesized sorbent were undergone for characterization using FESEM, EDX, XRD, FTIR, VSM, and TGA. Finally, real-time determination of quercetin was carried out using rose petal extract, integrating MMIP adsorption followed by HPLC detection. The results suggest that MMIP is a promising material for the real-time determination of quercetin from rose petal extract.