Magnetic Ionic Liquid Research Articles

An investigative study on different magnetic ionic liquids to be used as a lubricant and sealant in a vacuum pump

The aim of this work is to explore the possibility of using a magnetic ionic liquid as both a lubricant and sealant in a vacuum pump. The current lubricant in use is due to become obsolete due to incoming legislation on fluorinated compounds. The long-term goal is to use a magnetic field to hold the novel lubricant in place to create a better seal and thus reduce pressure more effectively whilst also lubricating the necessary mechanical parts. This paper explores physical properties (contact angle, viscosity, coefficient of friction (COF) and magnetism) and chemical properties (thermal stability and chemical stability as deduced by X-ray photoelectron spectroscopy (XPS)) of three ionic liquids based on imidazolium cations with varying length of alkyl side chain and a tetrachloroferrate anion along with two control lubricants which are used currently in the field and based upon perfluoropolyether (PFPE). The work shows that the three magnetic ionic liquids possess a lower COF value than the two control lubricants when measured using a Bruker UMT TriboLab suggesting they may perform better in the real life vacuum pump. Upon analysis of the three ionic liquid samples after tribological testing using XPS, it was shown that the ionic liquid samples displayed minor differences in spectra suggesting good stability with no obvious decomposition or degradation of the sample.

Neat and rapid preparation of hydrophobic magnetic ionic liquids composed of transition metal chelates featuring in situ formation capabilities in aqueous matrices

Nitrogen and oxygen-donor ligands comprised of alkylimidazoles, tertiary amides, and diglycolamides were employed to form transition metal chelates in the preparation of twelve magnetic ionic liquids. Viscosities as low as 198 cP were achieved.

Magnetic ionic crystals with light controllable mobility and CO2 physisorption/desorption.

Magnetic responsive ionic liquid (MIL) demonstrated an advanced photomobility in confined narrow spaces through the doping of photoresponsive azobenzene by the interplay of supramolecular π-cations. Moreover, reversible physisorption/desorption of CO2 was achieved based on the photocontrolled solid-liquid transitions of the mixtures. Our approach opens opportunities to obtain multi-stimuli response through the coordinated supramolecular interplay of each responsive component.

New biological monitoring method for 1-Naphthol and 2-Naphthol based on stir bar sportive dispersive micro extraction strategy using magnetic ionic liquid

Naphthalene is one of the few volatile bicyclic aromatic hydrocarbons and a white crystalline solid that can sublimate at room temperature. Since 2000, according to the results of animal studies on the carcinogenic effects of naphthalene, this substance has been recognized as a major problem for the environment and industrial environments. Exposure to naphthalene leads to kidney toxicity, hemolytic anemia and other health problems in the human body. The International Agency for Cancer has placed naphthalene in group 2B (probably carcinogenic to humans). 1- naphthol and 2- naphthol are biological urinary indicators of human exposure to naphthalene in work environments. For the first time in this research, the concentration of 1-naphthol and 2-naphthol in urine, which serves as an indicator of naphthalene exposure, was performed through a method known as magnetic ionic liquids-based disperse liquid–liquid microextraction (MIL-DLLME). Subsequently, the samples were analyzed using gas chromatography with a flame ionization detector (GC-FID). Additionally, the factors that influence the extraction of this metabolite, such as the quantity of extracting solvent, speed of mixing, proportion of salt added, duration of mixing, and pH value, were determined using both a two-level factorial test design and a central composite design. Calibration curves were developed under optimal conditions, and the validity of the developed microextraction method was studied with different statistical methods and the analysis of real samples. A strong linear correlation between the subpeak level and the corresponding concentrations was observed in the range of 0.5–0.002 μg/ml for 1-naphthol and 2.00–0.001 µg mL-1for 2-naphthol. In addition, the detection limit and quantitative detection limit for 1-naphthol were 0.0006 µg mL−1, 0.002 µg mL−1, and 0.0003 µg mL−1, 0.001 µg mL-1for 2-naphthol respectively. The MIL-DLLME extraction method can be regarded as an environmentally friendly microextraction technique since it eliminates the need for dispersing solvents and toxic organic solvents as extractants. Results showed that the proposed technique can be applied for biological monitoring of the biomarkers of naphthalene in the exposed workers during occupational or environmental assessments.

In–syringe homogenous liquid–liquid extraction combined with ionic liquid based dispersive liquid–liquid microextraction for the extraction of aflatoxin B1 from edible vegetable oil samples prior to HPLC-FLD analysis

Combination of in–syringe homogenous liquid–liquid extraction and ionic liquid based dispersive liquid–liquid microextraction has been employed for the extraction of aflatoxin B1 from edible vegetable oil samples. Firstly, a homogenous solution of oil sample containing the analyte and acetone (1.5 mL) is formed. Then, this homogenous state was broken by adding 100 µL of deionized water and the analyte was extracted into the produced fine droplets of acetone. In the following, acetone phase was taken and mixed with 65 µL of 1–hexyl–3–methylimidazolium tetrachloroferrate magnetic ionic liquid. The mixture was injected to sodium chloride solution (5 %, w/v) by a glass syringe. The ionic liquid droplets were collected by an external magnet and then, they were used for the determination step by high–performance liquid chromatography equipped with fluorescence detector. Under the optimum conditions, low limit of detection (13 ng kg−1) and quantification (44 ng kg−1), wide linear range (44–1 × 106 ng kg−1), acceptable extraction recovery (69 %), and good precision (relative standard deviations ≤ 4.0 %) were obtainable using the proposed method. These results verify the high capability of the proposed method for screening aflatoxin B1 in the various edible vegetable oil samples. Performing the method in syringe barrel provide easy collection of the extraction solvent without the need for centrifugation. Combination of homogenous liquid–liquid extraction with the microextraction procedure leads to obtain sensitive detection limits. The use of ionic liquid removed the necessity of centrifugation for separation of the extractant.

Harnessing the power of iron-alumina-based ionic liquid composites for simultaneous removal of Congo red dye and microplastics

Increased water shortage due to industrialization and population increase requires immediate action to address water contamination. Using dimethyl sulfate as a common anion, we synthesized imidazolium-based cations with varying chain lengths (Imidazole, 2-methyl imidazole, 2-ethyl imidazole, and 2-isopropyl imidazole). This allowed us to create novel room-temperature ionic liquids (RTILs) that can be stored and transported at room temperature. These RTILs were further modified to magnetic ionic liquid composites (MILCs) by adding iron-alumina composites. The synthesized RTILs and MILCs were further characterized using H-NMR, FTIR, TGA, SEM, and EDX techniques. The process of adsorption at varying parameters was studied for the removal of Congo red (CR) and microplastics using these materials. MILC-4 in results, demonstrated excellent adsorption effectiveness by reaching a high removal rate of 90% at a concentration of 10 ppm for CR. Furthermore, 10-μm polystyrene beads were successfully destroyed by the MILCs in about 30 min. Following the completion of 40 experimental data, an Artificial Neural Network (ANN) model with three layers was used to accurately predict the removal of CR dye from an aqueous solution by MILCs. For the prediction and modeling of CR dye removal, the minimal mean squared error (MSE) of 0.051 and coefficient of determination (R2) of 0.97 were discovered. These numerical findings demonstrate the amazing effectiveness of the synthesized MILCs in combating water pollution issues. Overall, the study emphasizes the necessity of cleaner production methods and sustainable solutions provided by MILCs for CR dye and microplastics removal from water bodies.

Study on the mechanism and recoverability of magnetically enhanced ionic liquid microemulsion for oily sludge treatment

Magnetic ionic liquid (MIL) 1-hexadecyl-3-methylimidazolium tetrachloroferrate (III) ([C16mim]FeCl4) with high surface activity and magnetic responsiveness was synthesized and chosen as surfactant for the preparation of MIL-microemulsion, which could be applied to the effective treatment of oily sludge based on its large specific surface area and good solubilization. Under the optimal conditions of MIL concentration of 3.0 wt%, stirring time of 40 min, stirring speed of 500 r/min and temperature of 25 °C, the residual oil rate reached up to 1.36%. Scanning electron microscope (SEM) and fourier infrared spectrometer (FT-IR) measurement proved the successful removal of crude oil. The interaction between [C16mim]FeCl4 and each component in crude oil was explored by interfacial tension (IFT) and quartz crystal microbalance with dissipation (QCM-D). The results demonstrated that MIL exhibited the lowest interfacial tension of 13.48 mN/m and the highest desorption rate of 28.18% with asphaltene. In addition, based on the magnetic responsiveness of MIL, the application of magnetic field made it possible for the recovery of MIL, which could be reused for the preparation of microemulsion. Even after 5 cycles, the residual oil content in the oily sludge remained below 1.92%. The controllable recovery and reuse of [C16mim]FeCl4 by external magnetic field expanded the application of MIL-microemulsion in the field of oily sludge treatment.

Lanthanide-Based Organic Salts: Synthesis, Characterization, and Cytotoxicity Studies.

The formulation of magnetic ionic liquids (MILs) or organic salts based on lanthanides as anions has been explored. In this work, a set of choline-family-based salts, and two other, different cation families, were combined with Gadolinium(III) and Terbium(III) anions. Synthetic methodologies were previously optimized, and all organic salts were obtained as solids with melting temperatures higher than 100 °C. The magnetic moments obtained for the Gd(III) salts were, as expected, smaller than those obtained for the Tb(III)-based compounds. The values for Gd(III) and Tb(III) magnetic salts are in the range of 6.55-7.30 MB and 8.22-9.34 MB, respectively. It is important to note a correlation between the magnetic moments obtained for lanthanides, and the structural features of the cation. The cytotoxicity of lanthanide-based salts was also evaluated using 3T3, 293T, Caco2, and HepG2 cells, and it was revealed that most of the prepared compounds are not toxic.

Thermosensitive magnetic ionic liquids with different heterocyclic moieties as draw solutes for forward osmosis

Thermosensitive magnetic ionic liquids (TMILs) with different heterocyclic rings, 1-butyl-3-methylimidazolium tetrachloroferrate ([BIM][FeCl4]), 1-butylpyridinium tetrachloroferrate ([BPY][FeCl4]), and 1-butyl-1-methylpyrrolidinium tetrachloroferrate ([BMP][FeCl4]), were synthesized and applied as effective draw solutes in forward osmosis (FO). These heterocyclic-based TMILs exhibited lower critical solution temperatures (LCST) and paramagnetic behaviors, contributing to their use as draw solutes by minimizing energy consumption during solute recovery. The LCSTs of 20 wt% [BIM][FeCl4], [BPY][FeCl4], and [BMP][FeCl4] aqueous solutions were approximately 77, 77, and 69 °C, respectively. Their magnetic susceptibilities at 300 K were 4.283, 4.309, and 4.294 emu g−1, respectively. The water flux of 20 wt% [BMP][FeCl4] was approximately 20.07 Lm−2 h−1 using the active layer-facing draw solution mode. Moreover, [BMP][FeCl4] generated a meaningful water flux against simulated brackish water (2000 ppm NaCl). Thus, the draw solute properties of TMILs containing different ring groups revealed that heterocyclic-based TMILs can be used as potential draw solutes for practical FO systems.

Magnetic ionic liquid catalyst functionalized with antimony (III) bromide for effective glycolysis of polyethylene terephthalate

In a previous study, we demonstrated the efficient depolymerization of polyethylene terephthalate (PET) through glycolysis using antimony (III) oxide, a commonly used catalyst in PET synthesis. In the present research, we introduce a novel approach involving the synthesis of a magnetic bifunctional ionic liquid, Fe3O4@PMIM.SbBr4, containing only 2.2 wt% of antimony. The aim is to reduce the required antimony dosage for the reaction and enable its facile recovery and reuse. By employing this catalyst in PET chemical recycling through glycolysis to generate bis (2-hydroxyethyl) terephthalate (BHET) monomer, we achieved 100% PET conversion with a 96.4% yield and selectivity for BHET. This outcome was obtained using a catalyst loading of 6.0 wt% at 200 °C and 0.6 bar in a high-pressure reactor. We explored the impact of catalyst loading on BHET yield and conducted a comparative assessment of the Fe3O4@PMIM.SbBr4 catalyst against antimony (III) bromide, and another synthesized unsupported antimony-containing ionic liquid. Our results revealed the superior catalytic activity of the magnetic ionic liquid catalyst in PET glycolysis. The utilization of this catalyst offers promising potential for PET glycolysis due to its effortless separation using an external magnet, ability to produce highly pure BHET, and recyclability for repetitive use.

MIL pretreatment on breaking hydrogen bond and swelling of coal: Experiment and simulation

Since direct combustion of low-rank coal is inefficient, expansion and dissolution are essential for better use of coal as an energy source. In this study, [C8mim]FeCl4 molecular configuration was synthesized by quantum chemical simulation method, and Magnetic ionic liquid (MIL) was used to modify coal to improve its swelling efficiency. The FT-IR and Raman properties of MIL were measured by simulation combined with practical methods. Dmol3 was used to study the hydrogen bond changes of coal molecules induced by MIL. When MIL interacts violently with organic matter in coal, the cross-linking network structure generated by non-covalent bonds is destroyed, resulting in the break of covalent bonds such as hydrogen bonds. Further experimental study shows that the pore structure and surface morphology of modified coal samples will be damaged, indicating that the MIL has the ability to dissolve hydrogen bonds and pretreat low-rank coal. It was found by TGA that MIL treatment can increase the tar yield of coal by up to 4.79% and reduce ash content by 3.51%. Through the integration of molecular modeling and experimental analysis, this study elucidates the fundamental principles underlying MIL pretreatment of coal, thereby providing a viable strategy for efficient quality improvement utilization of low-rank coal.

Study on low-rank coal surface wettability effect and recyclability of different anionic magnetic ionic liquids

Three types of magnetic ionic liquids (MIL) ([C12mim]FeCl4, [C12mim]2CoCl4, and [C12mim]2NiCl4) were chosen to study the enhancement of the quality and utilization of low-rank coal (LRC). The mechanism of coal wettability modification was explained from the macrocosm and microcosm points of view by contact angle, adhesion work, and adsorption studies, and the recovery of MIL was realized by salting out the separation-strong magnetic recovery (SMR). The contact angle and adhesion work results showed that [C12mim]FeCl4 had the greatest effect on coal wettability. The adsorption on the LRC surface was as follows: [C12mim]FeCl4 > [C12mim]2CoCl4 > [C12mim]2NiCl4. The adsorption thermodynamics indicated that MIL conformed to the Langmuir isotherm model, which is a spontaneous reaction of exothermic entropy minus physical adsorption. The adsorption kinetic process belong to the pseudo-second-order kinetic model and was influenced by the combined action of intraparticle and liquid film diffusion. From the contact angles and adsorption thermodynamics, it can be inferred that [C12mim]FeCl4 has the highest activity and can better adsorb onto the LRC surface, covering the oxygen-containing functional groups and reducing the wettability to achieve efficient quality improvement and utilization of LRC. Through the research of salting-out separation-SMR, the most suitable salting-out reagent was found to be NaCl, and the highest recovery was obtained when the concentration reached the solubility level. As the magnetic field strength increases, the recovery rate gradually increases. The recovery of [C12mim]FeCl4 was more than 90% when it reaches 1.5 T, and it could be recovered by magnetic separation.

Application of bio-metal-organic framework-based magnetic dispersive solid phase extraction for the extraction of polycyclic aromatic hydrocarbons from pasteurized milk

This work describes the development of a magnetic dispersive solid phase extraction method based on using bio–metal–organic framework (prepared from vitamin B3 and cobalt salt in water medium) as an efficient sorbent for the extraction of polycyclic aromatic hydrocarbons from milk samples. Briefly, the synthesized sorbent at mg–level is added to the supernatant phase after precipitating the proteins of milk sample and the mixture is vortexed. In the presence of magnetic field, the supernatant is removed and the sorbent particles containing the adsorbed analytes are eluted by using an appropriate organic solvent. The eluted analytes are more enriched using a magnetic ionic liquid–based dispersive liquid–liquid microextraction. After back–extraction of the analytes into n–hexane, the analytes are determined using gas chromatography–mass spectrometry. The experimental variables influencing the suggested method efficiency such as zinc sulfate solution concentration and volume, and vortexing time in proteins precipitating step, sorbent amount, adsorption time, ionic strength, elution solvent type and volume, desorption time, extraction solvent type and volume, and back–extraction solvent type, volume and time in extraction procedure were optimized by one parameter at a time strategy. Under the optimum conditions, low limits of detection (38–78 ng L−1) and quantification (130–260 ng L−1), high extraction recoveries (58–82%) and enrichment factors (145–205), and good precision (relative standard deviations ≤6.1%) were obtained. Finally, the developed method was effectively employed for quantification of polycyclic aromatic hydrocarbons in pasteurized milk samples.

Multifunctional Magnetoelectric Sensing and Bending Actuator Response of Polymer-Based Hybrid Materials with Magnetic Ionic Liquids.

With the evolution of the digital society, the demand for miniaturized multifunctional devices has been increasing, particularly for sensors and actuators. These technological translators allow successful interaction between the physical and digital worlds. In particular, the development of smart materials with magnetoelectric (ME) properties, capable of wirelessly generating electrical signals in response to external magnetic fields, represents a suitable approach for the development of magnetic field sensors and actuators due to their ME coupling, flexibility, robustness and easy fabrication, compatible with additive manufacturing technologies. This work demonstrates the suitability of magnetoelectric (ME) responsive materials based on the magnetic ionic liquid (MIL) 1-butyl-3-methylimidazolium tetrachloroferrate ([Bmim][FeCl4]) and the polymer poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE) for magnetic sensing and actuation device development. The developed sensor works in the AC magnetic field and has frequency-dependent sensitivity. The materials show voltage responses in the mV range, suitable for the development of magnetic field sensors with a highest sensitivity (s) of 76 mV·Oe-1. The high ME response (maximum ME voltage coefficient of 15 V·cm-1·Oe-1) and magnetic bending actuation (2.1 mm) capability are explained by the magnetoionic (MI) interaction and the morphology of the composites.

Continuous enrichment and trace analysis of tetracyclines in bovine milk using dual-functionalized aqueous biphasic system combined with high-performance liquid chromatography

Two PPG1000 based temperature-sensitive magnetic ionic liquid were synthesized and characterized. The temperature-sensitive magnetic ionic liquid aqueous biphasic system combined with HPLC was applied for the continuous enrichment and trace analysis of tetracycline antibiotics (TC) in bovine milk for the first time. High enrichment factors were achieved and the detection was highly sensitive. The trace analysis of TC was rapid, free of organic solvent, recyclable and magnetically assisted for phase separation. Under optimum conditions, wide linear ranges of 0.25-300 ng/mL for all TC, high enrichment factors of 217.7-231.4, good precisions with relative standard deviation in the range of 0.74-3.97%, very low limits of detection of 0.031-0.067 ng/mL, limits of quantification of 0.103-0.223 ng/mL, and good recoveries of 94.28-99.76% were acquired for the proposed analytical method. Real milk analysis was satisfactory. This developed analytical method is showing great potential for trace analysis of targeted analytes in foods and drinks.

Ultra-Low Viscosity and High Magnetic Susceptibility Magnetic Ionic Liquids Featuring Functionalized Diglycolic Acid Ester Rare-Earth and Transition Metal Chelates.

Magnetic ionic liquids (MILs) comprise a subcategory of ionic liquids (ILs) and contain a paramagnetic metal center allowing them to be readily manipulated by an external magnetic field. While MILs are popularly employed as solvents in catalysis, separations, and organic synthesis, most low viscosity combinations possess a hydrophilic character that limits their use in aqueous matrices. To date, no study has reported the synthesis and characterization of hydrophobic MILs with viscosities similar to those of hydrophilic MILs and organic solvents while simultaneously exhibiting enhanced magnetic and thermal properties. In this study, diglycolic acid esters are employed as ligands to chelate with paramagnetic metals to produce cations that are paired with metal chelates composed of hexafluoroacetylacetonate ligands to form MILs incorporating multiple metal centers in the cation and anion. Viscosity values below 31.6 cP were obtained for these solvents, the lowest ever reported for hydrophobic MILs. Solubilities in nonpolar solvents such as benzene were observed to be as high as 50% (w/v) MIL-to-solvent ratio while being insoluble in water at concentrations as low as 0.01% (w/v). Effective paramagnetic moment values for these solvents ranged from 5.33 to 15.56 Bohr magnetons (μB), with mixed metal MILs containing multiple lanthanides in the anion generally offering higher magnetic susceptibilities. MILs composed of ligands containing octyl substituents were found to possess thermal stabilities up to 190 °C. The synthetic strategies explored in this study exploit the highly tunable nature of the employed cation and anion pairs to design versatile ultra-low viscosity magnetoactive solvents that possess tremendous potential and applicability in liquid-liquid separation systems, catalysis, and microfluidics where the mechanical movement of the solvent can be easily facilitated using electromagnets.

Different anionic MIL structure optimization and molecular dynamics simulation of adsorption on coal surface

Surfactants can regulate the surface wettability of low-rank coal (LRC), resulting in highly efficient coal usage. Magnetic ionic liquids (MILs), a novel form of surfactants, provide environmental protection, excellent designability, and recyclability. In this study, quantum chemical simulations were performed to optimize the structures of [C12mim]FeCl4, [C12mim]2CoCl4, and [C12mim]2NiCl4 and the structural parameters were verified. The effect of MILs on the surface wettability of LRC was investigated by measuring the critical micelle concentration (CMC) using a conductivity technique and computing the adhesion work; thus, the micro-control mechanism of wettability was clarified. The wettability investigation revealed that the three different types of MILs had higher critical micelle concentrations as the temperature increased. Molecular dynamics modeling was utilized to calculate the adsorption process simultaneously, and the results demonstrated that the three different MILs may spontaneously complete adsorption on the surface of the LRC. Thermogravimetric and X-ray photoelectron spectroscopy analyses demonstrated that after the addition of the three different types of MILs, the oxygen-containing functional groups in the LRC were covered, the LRC surface became more hydrophobic, the water content of the LRC decreased, and the wettability of the LRC was effectively controlled.

Pyrolysis of cellulose in Fe‐[Bmim]OTf catalyst for selective production of 4,4‐dimethyl‐2‐cyclohexen‐1‐one

Abstract4,4‐dimethyl‐2‐cyclohexene‐1‐one is a valuable ketone compound, which is an important (±)‐cuparene and filicinic acid intermediate, but the synthetic method of 4,4‐dimethyl‐2‐cyclohexene‐1‐one is rarely mentioned in the marketplace. This article, a new method is proposed for the preparation of 4,4‐dimethyl‐2‐cyclohexen‐1‐one in high yield by selective catalytic pyrolysis of cellulose with FeCl3‐doped [Bmim]OTf magnetic ionic liquids. By studying the effect of temperature on the yield of pyrolysis products in [Bmim]OTf, the results showed that the optimal pyrolysis temperature of cellulose in [Bmim]OTf was 330°C and the maximum relative content of 4,4‐dimethyl‐2‐cyclohexen‐1‐one was 86.81%. In addition, different FeCl3/[Bmim]OTf mass ratios were found at 330°C. The relative yield of the target product reached a maximum (91.31%) when iron ions with excellent catalytic activity were introduced, at which the FeCl3/[Bmim]OTf mass ratio was 10%. At last, based on the basic model of cellulose monomer, the evolution pathway of ketones products was proposed, which provides ideas for the production of sustainable chemical products from cellulose.

Magnetic ionic liquid-based liquid-liquid microextraction followed by ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for simultaneous determination of neurotransmitters in human cerebrospinal fluid and plasma

A green, efficient and easy sample pretreatment method of magnetic ionic liquid-based liquid-liquid microextraction (MIL-based LLME) combined with a sensitive, rapid and precise analytical method of ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-QqQ/MS2) was developed to simultaneously - determining of neurotransmitters (NTs) in biosamples. Two magnetic ionic liquids (MILs), [P6,6,6,14]3[GdCl6] and [P6,6,6,14]2[CoCl4] tested, and the latter was selected as the extraction solvent due to its advantages of visual recognition, paramagnetic behavior and higher extraction efficiency. Facile magnetic separation of MIL containing analytes from matrix was realized by applying external magnetic field without rather than centrifugation. Experimental parameters that would influence the extraction efficiency, including type and amount of MIL, extraction time, speed of the vortex process, salt concentration, and environmental pH, were optimized obtained. The proposed method was successfully applied to the simultaneous extraction and determination of 20 NTs in human cerebrospinal fluid and plasma samples. Excellent analytical performance indicates the broad potential of this method for clinical diagnosis and therapy of neurological diseases.

Enhancement of Peroxidase Activity in Magnetic Ionic Liquids

Cytochrome c (Cyt-c) is a heme-containing protein that plays a vital role as an electron carrier in the mitochondrial respiratory chain. Cyt-c possesses peroxidase-like activity in the native state despite its six-coordinated heme iron. In this work, we studied the effect of magnetic ionic liquids (MILs) on the peroxidase activity of Cyt-c. Guaiacol oxidation was used to probe the activity of Cyt-c in the presence of cholinium (Ch)-based MILs such as [Ch][FeCl4] and [Ch]2[MnCl4]. A detailed kinetics and thermodynamics study on the activity of Cyt-c in the presence of MILs was investigated with the help of isothermal titration calorimetry (ITC), UV–vis, circular dichroism (CD), and fluorescence spectroscopy. The peroxidase activity of Cyt-c increases by twofold in the presence of [Ch][FeCl4] and only 20% in the presence of [Ch]2[MnCl4] IL. This correlates with the accessibility of the heme iron of Cyt-c. Molecular docking and molecular dynamic simulations were also employed to explore the mechanism of the interaction. The increase in the peroxidase activity of Cyt-c is attributed to the perturbation in the native sixth coordination bond of methionine-80 associated with the heme region of Cyt-c. Since not much work has been done on the enzymatic activity in the presence of MILs, we hope this will set the platform to use MILs to modulate and control enzymatic activity and catalysis.