Magnesium Ion Concentration Research Articles

Impact of total dissolved solids in drinking water on nutrient utilisation and growth performance of Murrah buffalo calves

This study was carried out to evaluate the effect of total dissolved solids (TDS) in drinking water on nutrient intake, utilisation and performance of growing Murrah buffaloes under tropical climatic conditions (maximum ambient temperature (Tmax)=10–42.6°C; relative humidity (RH)=10-100%). Twenty male Murrah buffalo calves were divided according to body weight (BW=220±36kg) into 5 groups viz. 557, 2571, 4467, 6113 and 8789 which were offered water containing TDS 557, 2571, 4467, 6113 and 8789mg/L, respectively for a period of 165 days. Animals in all groups were offered a total mixed ration (crude protein=10.3% and metabolisable energy=8.6MJ/kg dry matter) prepared from green oats, concentrate mixture and wheat straw in 20:35:45 proportion daily. Results revealed an increase in the concentrations of calcium (Ca), magnesium (Mg), sodium (Na) and chloride (Cl) ions with increasing levels of TDS in drinking water. Daily drinking water (L/100kg BW) and dry matter intake (kg/100kg BW) decreased (P<0.05) by 36.1% and 17.2% in group 8789 as compared to group 557. Similarly, average daily gain (g/d) and nitrogen intake (g/d) was lower (P<0.05) in groups 6113 and 8789 in comparison to all other groups. However, nutrient digestibility and concentration of major minerals (Na, Ca, K and Mg) in plasma showed non significant differences among the groups. Overall it can be concluded that TDS level >4500mg/L in drinking water adversely affected water and feed intake which ultimately resulted in reduced growth performance of Murrah buffalo calves.

Toxic effects of zinc ions on kinesin – Potential molecular cause of impaired intracellular transport

In healthy organisms the metabolism of the trace element zinc is well balanced. If this balance becomes destroyed the free zinc level might increase and cause toxic effects. The present study demonstrates that under definite conditions zinc ions are able to inhibit the ATPase activity of neuron-specific KIF5A (kinesin-1). Correspondingly, the motility activity of KIF5A also decreased. The inhibition rates have been found to depend on the magnesium ion concentration. Lowering the magnesium concentration weakens the inhibition. In addition, also decreases of temperature or increasing the ATP concentration result in reduced inhibition. Zinc ion-mediated inhibition of KIF5A activity might be one molecular cause contributing to impaired transport processes within brain and other organs in cases of zinc dyshomeostasis.

ВИВЧЕННЯ УМОВ ОТРИМАННЯ СПОЛУК МАГНІЮ ОСОБЛИВОЇ ЧИСТОТИ З ЙОГО ХЛОРИДУ

In the article questions of providing quality of important for new technology Chloride compounds and Magnesium oxide of high level of purity are considered.The processes of magnesium ions alkaline hydrolysis and its features within the borders of high concentration of magnesium ions are studied. It is shown that in such conditions alkaline solutions are formed, which contain magnesium complexes that can form magnesium hydroxide sediment with special properties: high sorption capacity to impurity ions of 3-d elements and significant filtration rate, which areespecially important in the technology of separation of such sediment. Based on results obtained in the study the recommendationson the technology of obtaining high-purity Chloride and Magnesium oxide are given. Recommendations regarding applicability of the research results andtechnological techniques for other high-purity compounds such as: Sodium chloride,Potassium and Calcium are given.

Optimization of lychee wine fermentation process using response surface methodology to reduce acetic acid content

Abstract: Acetic acid is the main component of the volatile acid in the wine. However, excessive amounts of acetic acid negatively affect wine quality. The study aimed to decrease acetic acid content produced by Saccharomyces cerevisiae fermentation after adding metal ion at different temperatures. Response surface methodology (RSM) was used to predict the optimum conditions for acetic acid removal. A central composite design was employed for the experiments and results were analyzed to obtain the best possible combination of fermentation temperature (X1: 16°C-24°C) and concentrations of potassium (X2: 0-12.0 mM), magnesium (X3: 0-8.0 mM), and calcium ions (X4: 0-0.2 mM) that would generate the minimum acetic acid in lychee wine at an initial acetic acid concentration of 1.5 g/L. Experimental data were fitted to a second-order polynomial equation using multiple regression analysis and analyzed using analysis of variance (ANOVA). During fermentation under pre-established conditions, the correlation coefficients R2 and Adj-R2 of the models for acetic acid removal were 0.9487 and 0.9007, respectively. After testing, the optimum conditions for acetic acid removal were determined as follows: fermentation temperature of 20°C; potassium, magnesium, and calcium ion concentrations of 10.1 mM, 6.1 mM, and 0.2 mM, respectively. The experimental acetic acid content of lychee wine under optimal conditions was found to be 0.309 g/L, which agreed well with the model-predicted value of 0.314 g/L. Keywords: lychee wine, acetic acid, fermentation, Saccharomyces cerevisiae, metal ion, response surface methodology DOI: 10.3965/j.ijabe.20160906.2270 Citation: Wu R N, Zhu P, Shang Y H, Zhong Q P. Optimization of lychee wine fermentation process using response surface methodology to reduce acetic acid content. Int J Agric & Biol Eng, 2016; 9(6): 223－230.

Assessment of the quality of groundwater for drinking purposes in the Upper West and Northern regions of Ghana.

BackgroundUnderground water is an important natural resource serving as a reliable source of drinking water for many people worldwide, especially in developing countries. Underground water quality needs to be given a primary research and quality control attention due to possible contamination. This study was therefore designed to determine the physico-chemical and bacteriological quality of borehole water in the Upper West and Northern regions of Ghana.MethodsThe study was conducted in seven districts in Ghana (including six in the Upper West region and one in the Northern region). The bacterial load of the water samples was determined using standard microbiological methods. Physico-chemical properties including pH, total alkalinity, temperature, turbidity, true colour, total dissolved solids (TDS), electrical conductivity, total hardness, calcium hardness, magnesium hardness, total iron, calcium ion, magnesium ion, chloride ion, fluoride ion, aluminium ion, arsenic, ammonium ions, nitrate and nitrite concentrations were determined. The values obtained were compared with the World Health Organization (WHO) standards for drinking water.ResultsThe recorded pH, total alkalinity and temperature ranges were 6.14–7.50, 48–240 mg/l and 28.8–32.8 °C, respectively. Furthermore, the mean concentrations of iron, calcium, magnesium, chloride, fluoride, aluminium, arsenic, ammonium, nitrate and nitrite were 0.06, 22.11, 29.84, 13.97, 0.00, 0.00, 0.00, 0.01, 2.09 and 0.26 mg/l, respectively. Turbidity, true colour, TDS and electrical conductivity of the water samples ranged from 0.13 to 105 NTU, 5 to 130 HU, 80.1 to 524 mg/l and 131 to 873 µS/cm, respectively. In addition, the mean total hardness value was found to be 178.07 mg/l whereas calcium hardness and magnesium hardness respectively were 55.28 and 122.79 mg/l. Only 14% of the water samples tested positive for faecal coliforms.ConclusionThe study revealed that only a few of the values for the bacteriological and physico-chemical parameters of the water samples were above the tolerable limits recommended by the WHO. This calls for regular monitoring and purification of boreholes to ensure good water quality.

A ribosomal strategy for magnesium deficiency

Under low-magnesium stress, bacteria suppress ribosome synthesis.

Transducin-activated cGMP-specific phosphodiesterase of external segments of bovine retinal rods: The influence of magnesium ions

The kinetic behavior of phosphodiesterase activated by transducin in a complex with a non-hydrolyzable GTP analogue (guanosine-5′-O-thiotriphosphate) was studied by the pH-metric method in preparations of light-adapted external segments of bovine retinal rods in a range of magnesium ion concentrations from 0.4 to 20 mM. These results indicate that when using the reaction media containing 10–15 mM Mg2+ ions, introduction of 2–4 mM ethylene glycol tetraacetic acid (a chelator of calcium ions) in the reaction medium induces only relatively small changes in the concentration of free magnesium ions that are not able to significantly influence the phosphodiesterase activity.

Role of magnesium on the biomimetic deposition of calcium phosphate

Biomimetic depositions of calcium phosphate (CaP) are carried out using simulated body fluid (SBF), calcifying solution and newly developed magnesium containing calcifying solution. Calcium phosphate has a rich phase diagram and is well known for its excellent biocompatibility and bioactivity. The most common phase is hydroxyapatite (HAp), an integral component of human bone and tooth, widely used in orthopedic and dental applications. In addition, calcium phosphate nanoparticles show promise for the targeted drug delivery. The doping of calcium phosphate by magnesium, zinc, strontium etc. can change the protein uptake by CaP nanocrystals. This work describes the role of magnesium on the nucleation and growth of CaP on Ti and its oxide substrates. X-ray diffraction studies confirm formation of HAp nanocrystals which closely resemble the structure of bone apatite when grown using SBF and calcifying solution. It has been observed that magnesium plays crucial role in the nucleation and growth of calcium phosphate. A low magnesium level enhances the crystallinity of HAp while higher magnesium content leads to the formation of amorphous calcium phosphate (ACP) phase. Interestingly, the deposition of ACP phase is rapid when magnesium ion concentration in the solution is 40% of calcium plus magnesium ions concentration. Moreover, high magnesium content alters the morphology of CaP films.

The classification, structure and functioning of Ago proteins in Eukaryotes.

Ago proteins are members of the highly specialized and conserved Argonaute family, primarily responsible for regulation of gene expression. As a part of RNA-induced silencing complexes (RISCs) Ago proteins are responsible for binding a short RNA and cleavage/inhibition of translation of target mRNAs. Phosphorylation may work as the switch between those two functions, but the role of magnesium ion concentration is also taken into consideration. Recent reports indicate that Ago proteins can interact with an mRNA and cause inhibition of translation without the participation of a short RNA. As key elements in RNA interference processes, Ago proteins are an important and intensively exploited area of research. Furthermore, these proteins are involved in the repair of DNA double-strand breaks by homologous recombination, modifications of chromatin, and alternative splicing. Their role in the cell cycle and senescence is also being studied. In addition, Ago expression is tissue-specific, which potentially may be used for diagnostic purposes. Understanding the mechanisms of Ago functioning is therefore crucial for understanding many cellular processes. The following article presents a detailed description of the Ago proteins including their post-translational modifications, recent data and hypotheses concerning their interactions with short RNAs and mRNAs as well as the mechanisms of siRNA/miRNA sorting into individual members of the Ago subfamily, and their role in eukaryotic cells. The latest classification of Ago proteins within the Argonaute family based on evolutionary studies and their possible interactions with DNA are also described.

Effects of dietary supplementation of Chinese herb medicine mixture on rumen fermentation, nutrient digestion and blood profile in goats

Twenty adult goats, fitted with ruminal cannulae, were used to investigate the effects of dietary supplementation of Chinese herbal medicine (CHM) mixtures on rumen fermentation, nutrient digestion and blood profiles. The wethers were assigned equally to one of the two dietary treatments according to a randomized controlled trial design. One dietary treatment was the basal diet, while the other was the CHM mixture diet consisting of the basal diet and the CHM mixture. Chinese Mosla, Atractylodes rhizome, Officinal Magnolia bark, white hyacinth bean and Liriope root tuber were mixed with a weight ratio of 8:4:4:4:5 to make up the CHM mixture. This mixture was added to the basal diet at 15 g/kg feed. The dietary supplementation of the mixture increased ruminal fluid concentrations of acetate, butyrate and total volatile fatty acids, and the ruminal activities of avicelase, carboxymethyl cellulase, cellobiase and xylanase. It also improved the ruminal effective degradability of dry matter, organic matter and amylase-treated ash-free neutral detergent fibre from the forage, apparent total tract digestibility of gross energy, dry matter, organic matter, crude protein, amylase-treated ash-free neutral detergent fibre, ash-free acid detergent fibre and calcium. Whole blood pH and concentrations of actual bicarbonate, buffer excess and magnesium ion, plasma concentrations of leptin, total protein, albumin, globulin, C-reactive protein, immunoglobulin A/G/M and complement 3/4 increased. Furthermore, plasma activities of nitric oxide synthase, glutathione peroxidase and total superoxide dismutase and plasma total antioxidative capacity increased. However, plasma concentrations of adrenocorticotropic hormone, aldosterone, cortisol, glucose, urea nitrogen and lactic acid and plasma activities of alanine transaminase, creatine kinase and lactate dehydrogenase decreased. These results indicated that the dietary supplementation of the CHM mixture exerted some positive regulating effects on the nervous system, the endocrine system and the immune system of goats, thus improving the functions of immunity and antioxidation, as well as the digestion and metabolism of nutrients. However, the CHM mixture did not affect their rumen fermentation patterns.Keywords: Chinese herbal medicine, rumen environmental parameter, digestibility, blood indicator, ruminant

Expression, purification and identification of a thermolysin-like protease, neutral protease I, from Aspergillus oryzae with the Pichia pastoris expression system

Neutral proteases are widely used in the textile, food and medical industries. This study was designed to obtain high expression levels of neutral protease I from Aspergillus oryzae 3.042 by using Pichia pastoris GS115 as the host strain for industrial purposes. The coding sequence of the target gene was modified, synthesized, and then cloned into the expression vector pHBM905BDM, which harbored the d1+2 × 201 AOX1 promoter and the MF4I leader sequence. The recombinant plasmid was transformed into Pichia pastoris GS115. The recombinant strain was used for high-density fermentation in a 4-L fermenter. The yield of the target protein reached 12.87 mg/mL, and the enzyme activity was approximately 49370 U/mL, which indicated that this enzyme was expressed in Pichia pastoris at a high level. The target protein was purified and characterized. Its optimum temperature and pH were 55 °C and 8.0, respectively. This enzyme was extremely sensitive to EDTA, which is consistent with the previous reports that it is a zinc-dependent metalloprotease. Our results indicated that low concentrations of zinc, calcium and magnesium ions stimulated the enzyme activity, whereas high concentrations inhibited its activity. In addition, calcium and magnesium ions increased the thermostability of the enzyme. All of the evidence indicated that this protease is a thermolysin-like peptidase.

ChemInform Abstract: Open‐Framework Oxysulfide Based on the Supertetrahedral [In4Sn16O10S34]12— Cluster and Efficient Sequestration of Heavy Metals.

Abstract[H3O]3[Heta]4.2 [H2dpp]0.3 [In4Sn16O10S32] ·33H2O is prepared by heating a 10:3:3:3 molar mixture of S, SnCl2·2H2O, In(NO3)3, and 1,3‐di(4‐pyridyl)propane (dpp) in ethanolamine (eta) (autoclave, 160 °C, 7 d; 25% yield).

Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system

Abstract In this paper, we report the inositide-driven formation of an inverse bicontinuous cubic phase with space group Ia3d ( Q II G , gyroid phase). The system under study consisted of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine at a molar ratio of 1:49, with a physiological concentration of magnesium ions at p H 7·4. The behaviour of the system was monitored as a function of temperature and pressure. The formation of the phase with Ia3d geometry was recorded repeatably at high pressure, and occurred more readily at higher temperatures. We conclude that the Ia3d phase formed is a thermodynamically stable structure, and that DSPIP is a potent source of membrane curvature that can drive the formation of mesophases with both 2- and 3D geometry.

Effects of major ions in recycled water on sulfide minerals flotation

The use of recycled water in flotation has significant effects on flotation and selectivity because the recycled water contains many dissolved compounds and ions that alter the chemistry of the system. In this study, the effects of calcium, magnesium and sulfate, which are abundant components in recycled water, were investigated on galena, sphalerite and pyrite flotation using pure minerals and a complex sulfide ore by conducting bench-scale flotation, microflotation and zeta potential measurements. The bench-scale flotation tests indicated that higher recoveries of galena and sphalerite are obtained when water is recycled, due to higher concentrations of ions in recycled water. However, there was a critical ion concentration for galena recovery. The microflotation tests showed that galena recoveries at different pHs in recycled water are lower than in tap and distilled water. Pure sphalerite recoveries improved in recycled water in the bench-scale test with ore. Pure pyrite was depressed with recycled water as well. Zeta potentials became less negative with increased calcium and magnesium ion concentrations due to their adsorptions, and the presence of sulfate ions was seen to increase the negative charges of sphalerite and pyrite, conversely for galena.

An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.

The shikimate pathway of bacteria, fungi, and plants generates chorismate, which is drawn into biosynthetic pathways that form aromatic amino acids and other important metabolites, including folates, menaquinone, and siderophores. Many of the pathways initiated at this branch point transform chorismate using an MST enzyme. The MST enzymes (menaquinone, siderophore, and tryptophan biosynthetic enzymes) are structurally homologous and magnesium-dependent, and all perform similar chemical permutations to chorismate by nucleophilic addition (hydroxyl or amine) at the 2-position of the ring, inducing displacement of the 4-hydroxyl. The isomerase enzymes release isochorismate or aminodeoxychorismate as the product, while the synthase enzymes also have lyase activity that displaces pyruvate to form either salicylate or anthranilate. This has led to the hypothesis that the isomerase and lyase activities performed by the MST enzymes are functionally conserved. Here we have developed tailored pre-steady-state approaches to establish the kinetic mechanisms of the isochorismate and salicylate synthase enzymes of siderophore biosynthesis. Our data are centered on the role of magnesium ions, which inhibit the isochorismate synthase enzymes but not the salicylate synthase enzymes. Prior structural data have suggested that binding of the metal ion occludes access or egress of substrates. Our kinetic data indicate that for the production of isochorismate, a high magnesium ion concentration suppresses the rate of release of product, accounting for the observed inhibition and establishing the basis of the ordered-addition kinetic mechanism. Moreover, we show that isochorismate is channeled through the synthase reaction as an intermediate that is retained in the active site by the magnesium ion. Indeed, the lyase-active enzyme has 3 orders of magnitude higher affinity for the isochorismate complex relative to the chorismate complex. Apparent negative-feedback inhibition by ferrous ions is documented at nanomolar concentrations, which is a potentially physiologically relevant mode of regulation for siderophore biosynthesis in vivo.

136 - A Member of Solute Carrier Family SLC41 Regulates the Interaction of Magnesium and Mesenchymal Stromal Cells During Osteogenic Differentiation

As the second most abundant intracellular cation, magnesium exists mostly in bone and the amount of magnesium is dynamically regulated by skeletal remodeling. Previous studies showed that accelerating bone mass loss occurs in post-menopause animals when their magnesium intake is insufficient and increased magnesium intake alleviates the osteoporotic symptoms. However, high magnesium status has been reported to lead to mineralization defects possibly due to the magnesium substitution for calcium in hydroxyapatite structure. In the present study, we used murine and human mesenchymal stromal cells (MSCs) to investigate the effect of high extracellular magnesium concentration on osteogenic differentiation and the role of magnesium transporters in the mineralization process. We verified the differentiation efficiency by observing cell morphology, expression profiling of osteogenic marker genes, and performing alizarin red staining (Fig. 1) . The results showed that high concentration of extracellular magnesium ion inhibited mineralization during osteogenic differentiation of MSCs. Gene expressions of osterix, type I collagen alpha 1, and alkaline phosphatase were downregulated, as well as osteopontin and osteocalcin were upregulated in MSCs treated with high concentration of magnesium. siRNA treatment targeting the specific magnesium transporter, which is also a member of solute carrier family SLC41, accelerated the mineralization process and ameliorated the inhibition of mineralization caused by high concentration of magnesium. The sensitivity to extracellular magnesium was also diminished after the knockdown. Pathway analysis of differentially expressed genes indicated that Wnt signaling is involved in the regulation mediated by the magnesium transporter. The study suggests that caution should be taken when magnesium supplement used as a therapy for bone mass loss and provide insights into the design of magnesium alloys for implantation.

Probing the ATP Site of GRP78 with Nucleotide Triphosphate Analogs.

GRP78, a member of the ER stress protein family, can relocate to the surface of cancer cells, playing key roles in promoting cell proliferation and metastasis. GRP78 consists of two major functional domains: the ATPase and protein/peptide-binding domains. The protein/peptide-binding domain of cell-surface GRP78 has served as a novel functional receptor for delivering cytotoxic agents (e.g., a apoptosis-inducing peptide or taxol) across the cell membrane. Here, we report our study on the ATPase domain of GRP78 (GRP78ATPase), whose potential as a transmembrane delivery system of cytotoxic agents (e.g., ATP-based nucleotide triphosphate analogs) remains unexploited. As the binding of ligands (ATP analogs) to a receptor (GRP78ATPase) is a pre-requisite for internalization, we determined the binding affinities and modes of GRP78ATPase for ADP, ATP and several ATP analogs using surface plasmon resonance and x-ray crystallography. The tested ATP analogs contain one of the following modifications: the nitrogen at the adenine ring 7-position to a carbon atom (7-deazaATP), the oxygen at the β-γ bridge position to a carbon atom (AMPPCP), or the removal of the 2’-OH group (2’-deoxyATP). We found that 7-deazaATP displays an affinity and a binding mode that resemble those of ATP regardless of magnesium ion (Mg++) concentration, suggesting that GRP78 is tolerant to modifications at the 7-position. By comparison, AMPPCP’s binding affinity was lower than ATP and Mg++-dependent, as the removal of Mg++ nearly abolished binding to GRP78ATPase. The AMPPCP-Mg++ structure showed evidence for the critical role of Mg++ in AMPPCP binding affinity, suggesting that while GRP78 is sensitive to modifications at the β-γ bridge position, these can be tolerated in the presence of Mg++. Furthermore, 2’-deoxyATP’s binding affinity was significantly lower than those for all other nucleotides tested, even in the presence of Mg++. The 2’-deoxyATP structure showed the conformation of the bound nucleotide flipped out of the active site, explaining the low affinity binding to GRP78 and suggesting that the 2’-OH group is essential for the high affinity binding to GRP78. Together, our results demonstrate that GRP78ATPase possesses nucleotide specificity more relaxed than previously anticipated and can tolerate certain modifications to the nucleobase 7-position and, to a lesser extent, the β-γ bridging atom, thereby providing a possible atomic mechanism underlying the transmembrane transport of the ATP analogs.

Open-Framework Oxysulfide Based on the Supertetrahedral [In4Sn16O10S34](12-) Cluster and Efficient Sequestration of Heavy Metals.

The new ion-exchange oxy-sulfide material has a three-dimensional open framework comprising the pseudo-T4 supertetrahedral [In4Sn16O10S34](12-) cluster. This material has large pores and is a fast ion exchanger. It exhibits high selectivity in sequestering heavy metal ions from aqueous solutions including solutions containing heavy concentrations of sodium, calcium, ammonium, magnesium, zinc, carbonate, phosphate, and acetate ions. Moreover, the ion-exchange efficiency in competitive ion-exchange experiments involving mixtures of metal ions is significantly higher than for solutions of single metal ions.

Daily magnesium fluxes regulate cellular timekeeping and energy balance.

Circadian clocks are fundamental to the biology of most eukaryotes, coordinating behaviour and physiology to resonate with the environmental cycle of day and night through complex networks of clock-controlled genes. A fundamental knowledge gap exists, however, between circadian gene expression cycles and the biochemical mechanisms that ultimately facilitate circadian regulation of cell biology. Here we report circadian rhythms in the intracellular concentration of magnesium ions, [Mg(2+)]i, which act as a cell-autonomous timekeeping component to determine key clock properties both in a human cell line and in a unicellular alga that diverged from each other more than 1 billion years ago. Given the essential role of Mg(2+) as a cofactor for ATP, a functional consequence of [Mg(2+)]i oscillations is dynamic regulation of cellular energy expenditure over the daily cycle. Mechanistically, we find that these rhythms provide bilateral feedback linking rhythmic metabolism to clock-controlled gene expression. The global regulation of nucleotide triphosphate turnover by intracellular Mg(2+) availability has potential to impact upon many of the cell's more than 600 MgATP-dependent enzymes and every cellular system where MgNTP hydrolysis becomes rate limiting. Indeed, we find that circadian control of translation by mTOR is regulated through [Mg(2+)]i oscillations. It will now be important to identify which additional biological processes are subject to this form of regulation in tissues of multicellular organisms such as plants and humans, in the context of health and disease.

Critical parametric influence on microalgae cultivation towards maximizing biomass growth with simultaneous lipid productivity

Enhancing microalgae biomass productivity through different abiotic and environmental factors optimization is crucial. Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was studied to evaluate the specific influence of eight important factors (light, pH, temperature, carbon concentration, nitrates, phosphates, magnesium ion concentration and carbon source) on the biomass production using three levels of factor (21 × 37) variation with experimental matrix [L18-18 experimental trails]. All the factors were assigned with three levels except light illumination (21). Substantial influence on biomass productivity is observed with carbon concentration contributing 16.8%, followed by nitrates 12.8% and light 9.3%. Experimental setup eight (Light, pH-8.5, Temperature 25°C, Carbon concentration 10 g/l, nitrates 1.5 g/l, phosphates 0 g/l, magnesium 150 mg/l, Carbon source (glucose)) showed maximum biomass growth (5.26 g/l) and good substrate degradation (63%, COD removal efficiency) contributing to carbohydrate production (257 mg/g biomass) which is further converted to lipids (20% Total lipid and 10% Neutral lipids). Chlorophyll (a, b), carbohydrates composition, FAME analysis for lipid percentage were monitored during process operation. Elemental analysis reveals that the carbon to hydrogen and oxygen ratio present in dried algal biomass can be hydrothermally liquefied (HTL) to produce biocrude.