Blood Serum Magnesium Research Articles

Методологія профілактики великих акушерських синдромів

Aim - to develop a method of prevention of major obstetric syndromes (MOS) from the standpoint of a single genesis determined by morphofunctional disorders in the fetoplacental system (FPS), with an assessment of effectiveness by methodological standards of evidence-based medicine Materials and methods. The group I - 32 pregnant women, prevention of MOS was carried out with low doses of acetylsalicylic acid (ASA) at 100-150 mg per os once a day, from 12 to 36 weeks of pregnancy; the group II - 31 pregnant women, who from 6 to 16 weeks were treated prophylactically with progesterone according to the scheme: 200 mg 1 time per day per vagina every day; the group III - 36 pregnant women who received prophylactic monotherapy, consisting of taking magnesium in combination with vitamin B6 for three courses of 6 weeks each; the group IV - 56 pregnant women who refused any preventive measures; the control group (CG) - 50 healthy pregnant women. Тhe effectiveness of prophylactic therapy was evaluated by dynamic examination of the pregnant groups of the study, which included the determination of the following indicators: placental alpha microglobulin (PAMG); placental growth factor; placental protein (PAPP-A), blood leukocyte elevation index, allergy index, concentration of magnesium in blood serum. To objectify the effectiveness of the developed method, the standards of evidence-based medicine were applied. Statistical processing of research results was carried out using standard Microsoft Excel 5.0 and Statistical 6.0 programs. Results. In pregnant women of the group IV, the level of the fibronectin (521±17 μg/ml) increased by an average of 17%, compared to patients of the groups I-III (355±12 μg/ml, 361±13 μg/ml, 368±16 μg/ml; p<0.05), which led to a subsequent increase in the frequency of spontaneous premature births in patients of the group IV; the level of PAMG exceeds the value in the CG by 7.7 times in the group IV; in the group III by 1.7 times, and in pregnant women of groups I and II, it was 25.2±3.8 ng/ml and 26.4±4.2 ng/ml, respectively, against 177±18 ng/ml in group IV (p<0.05). The concentration of the PAPP-A was reduced from the early stages of pregnancy in all studied groups - 6.9±1.8 mg/l, 8.8±1.9 mg/l, 9.2±2.1 mg/l, 7.5±1.6 mg/l (according to the groups I-IV) versus 15.7±2.5 mg/l in the CG (р<0.05). When using the magnesium drug (III group), there are differences with the group IV in most indicators. Conclusions. Methods for the prevention of a complicated course in the high-risk groups for the development of vasculitis when studying personalized and predictive value, which made it possible to divide them in order from more effective to less effective: the method with the appointment of low doses of ASA was in the first place; on the II - prophylactic appointment of micronized vaginal progesterone; on the III - the use of magnesium. The research was conducted according to principles of Declaration of Helsinki. Protocol of research was proved by local ethical committee, mentioned in institution’s work. A informed sonsennt was collected in order to carry out the research. The author is stating no conflict of interests is declared.

Risk stratification of drug-induced long QT syndrome caused by class III antiarrhythmic drugs

Aim. To develop a personalized risk assessment score for the development of drug-induced QT interval prolongation while taking class III antiarrhythmic drugs (AAD).Methods. We studied data from 110 patients with coronary artery disease and/or hypertension, who had heart arrhythmias and were taking class III AAD (amiodarone or sotalol) in a cardiology department. All patients underwent clinical, laboratory and instrumental studies, including history taking, 12-lead electricardiography recording, biochemical blood test, determination of the levels of neuronal NO-synthase (NOS1) and adapter protein of neuronal NO-synthase (NOS1AP) in blood plasma by ELISA, as well as the determination of polymorphisms G84A of the NOS1 gene using polymerase chain reaction. In order to stratify the risk of drug-induced QT interval prolongation, the method of linear discriminant analysis with stepwise inclusion was applied. The training sample consisted of 70 patients (63.6%), the test sample of 40 patients (36.4%). The score was developed on a training sample, and the testing was performed on a test sample with the construction of an ROC curve, calculation of AUC, sensitivity, and specificity.Results. The training and test samples were comparable in terms of the main clinical and anamnestic parameters and features of the pharmacological history. Patients with QT interval prolongation had significantly lower levels of magnesium (p=0.001), NOS1 (p=0.015) and NOS1AP (p=0.035). The discriminant analysis algorithm was stopped at the fourth step, as a result of which four statistically significant predictors were included in the model: thiazide or loop diuretic intake, blood serum magnesium level, plasma NOS1 and NOS1AP levels, each of which was assigned a certain number of points according to the received standardized coefficients. When conducting an ROC analysis on the initial sample, a threshold value of the scale of 6 points was obtained (AUC 0.848 (0.759 0.937, p=0.002), sensitivity 73.81%, specificity 85.71%). The use of the scale on the test sample showed sensitivity of 77.27%, specificity of 77.77% and AUC of 0.834 (0.721 0.965, p=0.001), which corresponds to the good quality of the prognostic model.Conclusion. Patients with a total score of ≥6 points have a high risk of drug-induced QT interval prolongation while taking class III AAD.

Morphological changes in the rat’s liver of different age after administration of magnesium chloride

The aim of the work was to study the morphological changes in the rat’s liver of different ages after prolonged administration of magnesium chloride. The experiments were performed on 48 Wistar male rats at 3 and 15 months of age. Experi mental animals, in addition to the standard diet, received magnesium chloride daily for 21 days at a dose of 50 mg / kg of body weight. Histological preparations were made from the liver tissue according to the standard method. The liver morphometry was performed on digital images using the computer program Image J. The content of magnesium cations was determined in the serum and suspension of red blood cells. Based on the results of our studies, it can be assumed that the administration of magnesium chloride activates the processes of physiological regeneration and functional activity of the liver parenchyma in rats of different ages. This is evidenced by an increase in the number of binuclear cells and nucleolus in the nucleus of hepatocytes, an increase in the nuclear-cytoplasmic and nucleolar-nuclear ratio. The relative area of the sinusoid network, the number and density of connective tissue cells were increased in the liver of 3-month-old experimental rats. This may indicate an increase of trophic and protective activity of the stroma in this organ. Experimental rats (15-month-old animals) revealed a moderate increasing in the erythrocyte suspension and blood serum magnesium content. The administration of magnesium chloride has the morphological features indicating an increasing of the physiological regeneration and activity of the liver parenchyma in young (to a greater extent) and adult animals.

Magnesium and vitamin B2 status of children with bronchial asthma and obesity

Insufficiency or deficiency of some micronutrients may be additional modifying factors that influence the pathogenesis of the disease and the effectiveness of standard pharmacotherapy. The aim of the study - to evaluate the level of magnesium and vitamin B2 in blood serum of patients with bronchial asthma and obesity in order to develop methods for individual correction of deficiency. Material and methods. The study included 51 children aged 12-17 years. The first group included 23 patients (12 girls and 11 boys) with asthma with comorbidities (obesity), and the second group consisted of 28 children (10 girls and 18 boys) with obesity. The concentration of magnesium in blood serum was determined by a colorimetric method without deproteinization, and vitamin B2 - by an immunological microbiological method. Results and discussion. When analyzing the concentration of magnesium in blood serum of the examined children, it was found that in patients with bronchial asthma and obesity, a reduced content of this mineral was observed in 15 (65.2%) patients. The average magnesium concentration was 0.66±0.02 mmol/l at a rate of 0.7-1.2 mmol/l. A statistically significant decrease in the magnesium level in children suffering from asthma and obesity was noted, compared with the level in children with obesity (0.66 [0.57; 0.73] vs 0.71 [0.67; 0.73] mmol/l, р<0.05). Low serum magnesium levels in obese patients were detected more rarely (р<0.05) - only in 6 (21.4%) children, mostly in patients with grade III obesity. The remaining 22 (78.6%) children had magnesium level within the normal range. Patients with low serum magnesium levels showed increased irritability, sleep disturbance, loss of memory and concentration. Vitamin B2 levels in all examined children were within the normal range (137-370 ng/ml). Conclusion. The results indicate a decrease in the concentration of magnesium and normal levels of vitamin B2 in serum in patients with bronchial asthma and obesity. Low serum magnesium levels were observed in children with low bronchial asthma control. To increase the effectiveness of therapy and control the symptoms of bronchial asthma, especially when combined with obesity, correction of the accompanying magnesium deficiency is necessary.

Determination of blood serum calcium, inorganic phoshorus and magnesium in different productive stages of holstein dairy cows

Introduction. The aim of this study was to determine and compare levels of blood serum calcium, inorganic phosphorus and magnesium in the peripartal period and during mid lactation in Holstein dairy cattle. Materials and Methods. Blood samples were collected from 12 cows in late pregnancy, 12 early lactation cows, and 12 mid lactation cows. Results and Conclusions. Serum calcium and inorganic phosphorus in blood of dairy cows in early and mid lactation were significantly lower compared to the values in the blood serum of dairy cows in late pregnancy, probably indicating the increased use of these macro elements by the mammary gland at the early stages of lactation. When the cows were in the lactation period, blood serum magnesium levels were significantly lower compared to the values of dairy cows during late pregnancy, probably indicating the increased use of magnesium by the dairy cows during lactation. The results show that the homeostasis of the macro elements examined in the blood of transition and mid lactation dairy cows was maintained, suggesting their adequate supply from alimentary sources.

Correlation between gamma-background of environment and indicators of electrolyte metabolism in patients with arterial hypertension depending on the variant of antihypertensive therapy

Arterial hypertension and ischemic heart diseases continue to lead among cardiovascular diseases. In this regard, an importance of fixed-combination antihypertensive therapy is noted. There is an evidence that in years of high solar activity in a group of healthy persons and patients with arterial hypertension and ischemic heart disease reduces the quality of professional activity, cardiovascular diseases exacerbate so that some patients end up developing a heart attack or stroke. There was revealed the correlation between γ-background dynamics of the external environment (within the boundaries of the regional standards) and indicators of electrolyte metabolism in patients with arterial hypertension with different temperament and anxiety, which take an empirical variant of antihypertensive therapy or another variant targeted to the blockade of temperamental peculiarities of psychosomatic status, the most effective antihypertensive therapy was determined. When the power of γ-background in medium was increased, the concentration of potassium and magnesium in blood serum and erythrocytes decreased, but the concentration of sodium increased as the erythrocyte resistance decreased. These shifts are closely related to the increase in osmolar pressure of blood plasma, permeability of erythrocyte membranes for sodium ions, and hemolysis (low resistance of red blood cells, increased indirect bilirubin), and hepatocytes (increase of direct bilirubin). On the background of the targeted antihypertensive therapy the severity of the processes is low and nearly similar to those in healthy persons of corresponding temperament that is the evidence of high efficiency of targeted treatment option.

Determination of Magnesium in Blood Serum by Using Carbon Paste Ion Selective Electrode Based on Multi-Walled Carbon Nanotubes and Nano Silicon

The number of trace metallic elements of biological importance is increasing and a wide range of elements need to be analyzed in blood, urine and tissues. There is often a natural limitation of sample volume particularly in the analysis of blood and tissue digest samples.

Dynamics of content of some minerals in teenagers’ organisms with cardiovascular system pathology and chronic tonsillitis

The article presents basic features of the mineral metabolism in adolescent children suffering from chronic tonsillitis and secondary affection of the cardiovascular system in disease dynamics. Aim of this work was to study the dynamics of zinc, iron, potassium and magnesium in adolescents with disorders of the cardiovascular system and chronic tonsillitis. Materials and methods . 63 patients with chronic tonsillitis were examined, among them – 31 children suffered from chronic tonsillitis without pathology of the cardiovascular system (I group), 32 – were patients with disorders of the cardiovascular system on the background of chronic tonsillitis (II group). Determination of concentration of trace elements of iron, zinc, potassium, magnesium in blood serum was measured by atomic absorption spectrophotometry. Results. The content of trace elements zinc, iron, minerals potassium and magnesium was analyzed. It breaches of macro- and trace element composition in the blood serum of children with chronic tonsillitis in the form of lower concentrations of iron and zinc in patients with damage to the cardiovascular system, and in patients without cardiac disease. It should be noted that children with tonsillogenyc cardiac lesions had a significant decrease in the level of macro-magnesium during hospitalization compared to children without cardiovascular pathology. After treatment, levels of such elements as iron, zinc, magnesium were not normalized. Moreover, significant changes were observed in patients with heart disease on the background of chronic tonsillitis. After the standard treatment, concentrations of iron and zinc in both groups, and magnesium in children from the second group remained at a low level, compaed with children without cardiac disease. Conclusion. The analysis of micro and macro elements in the serum of children with chronic tonsillitis, showed a significant decrease of iron and zinc concentration in the first days of hospitalization in both groups of respondents, and magnesium in children from the second group.

Ionized magnesium in plasma and erythrocytes for the assessment of low magnesium status in alcohol dependent patients

BackgroundStudies on the homeostasis of magnesium in alcohol-dependent patients have often been characterized by low hypomagnesemia detection rates. This may be due to the fact that the content of magnesium in blood serum constitutes only 1% of the average magnesium level within the human body. However, the concentration of ionized magnesium is more physiologically important and makes up 67% of the total magnesium within a human organism. There are no data concerning the determination of the ionized fraction of magnesium in patients addicted to alcohol and its influence on mental health status. MethodsThis study included 100 alcohol-dependent patients and 50 healthy subjects. The free magnesium fraction was determined using the potentiometric method by means of using ion-selective electrodes. The total magnesium level was determined by using a biochemical Indiko Plus analyzer. In this study, different psychometric scales were applied. ResultsOur results confirm the usefulness of ionized magnesium concentrations in erythrocytes and plasma as a diagnostic parameter of low magnesium status in alcohol-dependent patients. ConclusionsThe lower the concentration of ionized magnesium, the worse the quality of life an alcohol-dependent person might experience. In the case of total magnesium, no such correlation was determined.

Homocysteine as a Diagnostic and Etiopathogenic Factor in Children with Autism Spectrum Disorder

Substantial characteristics of autism are cognitive and psychophysical disorders. Etiopathogenetic factors are thought to be responsible for development of autism in children with genetic predisposition as well as have their effect on the severity of the disorders. The main problem of early identification of patients affected by autism spectrum disorder is that there are no clear diagnostic criteria. The aim of our study was assessment of hair magnesium and serum homocysteine concentrations in children with autism. The presented work is a continuation of previous study in which we investigated the influence of disturbances in magnesium and homocysteine levels in children with autism, performed on a new, larger group of patients. One hundred and forty children had hair magnesium levels analyzed, as well as blood serum levels of homocysteine and magnesium. Hair magnesium analysis was performed using a flame atomic absorption spectrometer, blood serum homocysteine determination was performed using a radioimmunological method, and blood serum magnesium level was determined using a biochemical method. Our research showed normal magnesium blood levels and significantly high homocysteine levels and very low hair magnesium levels. Low concentration of hair magnesium progresses with age. Our hypothesis is that magnesium deficiency, as a relevant epigenetic factor, might be decreasing methylation of homocysteine, therefore decreasing genome transcription and lowering the synaptic plasticity. We suggest that analysis of hair magnesium and serum homocysteine levels might be useful in identification of children with autism spectrum disorder, as well as control of its treatment. Obtained results and performed analysis might therefore justify supplementation of magnesium among children with autism.

The characteristics of magnesium homeostasis and remodeling of heart in patients with ischemia of myocardium against the background of non-differentiated dysplasia of connective tissue

The study was carried out to evaluate dynamics of level of magnesium of blood serum and structural functional parameters of heart in patients with acute myocardium infarction developed against the background of non-differentiated dysplasia of connective tissue. The study included 90 patients with myocardium infarction with Q wave and with and without nondifferentiated dysplasia of connective tissue. The control group consisted of healthy persons and comparison group included patients with non-differentiated dysplasia of connective tissue without cardiovascular pathology. The clinical and phenotype examination including echocardiography testing of heart, detection of level of magnesium in blood serum in dynamics of myocardium infarction course were implemented. In patients with myocardium infarction, independently of occurrence of non-differentiated dysplasia of connective tissue during the first day of study decreasing of level of magnesium in blood serum was observed. In the group of patients with myocardium infarction, and non-differentiated dysplasia of connective tissue, up to twenty-eighth day, reliable decreasing of magnesium occurred as compared with control group and comparison group. In group of patients with myocardium infarction and non-differentiated dysplasia of connective tissue expressed increase of size of cavity of left ventricle was established including decreasing of its pumping function in combination with tendency to less expressed increasing of index of myocardium mass of left ventricle as compared with patients with myocardium infarction and without non-differentiated dysplasia of connective tissue. In patients with myocardium infarction occurring against the background of non-differentiated dysplasia of connective tissue hypomagnesemia is associated with development of desadaptive alternative of post-infarction remodeling of heart characterized by prevalence of processes of dilatation of cavity of left ventricle with decreasing of contractive capacity over processes of hypertrophy of myocardium.

Evaluation of the relationship between concentration of endothelin-1 and magnesium in the blood and the severity of ischemic heart disease

Aim. To study the relationship between concentrations of endothelin-1 and magnesium in blood depending on functional class and old myocardial infarction in patients with stable angina.Methods. The study included 58 patients with ischemic heart disease, II and III functional class stable angina. 19 of these patients suffered a myocardial infarction in the past. The control group consisted of 25 healthy volunteers. Endothelin-1 level in blood serum was determined by enzyme immunoassay, the concentration of magnesium by colorimetric method.Results.Endothelin-1 level in the general group of patients with stable angina was 1.28±0.23 fmol/ml and was significantly higher than that of control group (0.52±0.13 fmol/ml, pConclusion. In patients with II-III functional class stable angina statistically significant increase in level of endothelin-1 and a decrease in the concentration of magnesium in blood serum compared with the control group was revealed; a negative correlation between endothelin-1 and magnesium levels in the blood, which is characterized by increasing the degree of correlation depending on the severity of the ischemic heart disease clinical form was established.

Состояние проницаемости гематосаливарного барьера при красном плоском лишае слизистой оболочки рта

The aim of the work was to study the permeability of hematosalivary barrier for mineral elements and the assessment of its role in the pathogenesis and clinical course of lichen planus of the oral mucosa. A total of 231 people were surveyed, which are divided into 6 groups depending on the clinical form of the disease and a control group. The concentrations of the mineral elements (zinc, copper and magnesium) in the blood serum and oral liquid are identified by atomic-absorption spectrophotometry. For each mineral element calculated coefficient ratio to reflects the operation of the hematosalivary barrier, which is the ratio of the concentration in the blood serum and oral fluid. Patients with oral lichen planus were found a violation of the permeability of hematosalivary barrier for some mineral elements, which is important in the pathogenesis of the disease. It appears as opposite changes of the mineral composition of blood serum and oral fluid, which have correlation with the severity of the clinical course of the disease. With increasing severity of the clinical course observed a significant decreasing of zinc, copper and a statistically significant increasing of magnesium in blood serum and oral fluid, which aggravates the severity of the clinical course of the oral lichen planus.

Evaluation of safety of magnesium of sulphate therapy in neonates with birth asphyxia

Background: Incidence of perinatal asphyxia is about 1 to 1.5% in most centres of world and is usually related to gestational age and birth weight. The incidence is higher in term infants of diabetic and toxaemic mothers. Intrauterine growth retardation, antepartum hemorrhage and breech presentation are also associated with an increased incidence of asphyxia. Methods: This is a prospective study carried out in Basveshwar & Sangmeshwar Teaching & General Hospitals from 1.12.2007 to 31.5.2009 on 80 terms, appropriate for gestational age babies. They all suffered severe birth asphyxia, of whom 40 formed control group & 40 formed study group. All were treated for birth asphyxia and its complications, except that the study group received injection of magnesium sulphate 250 mg/kg within half an hour of birth and subsequently 125 mg/kg at 24 & 48 hrs of life. Thereafter, they were monitored for effects on vital parameters and immediate outcome. Results: The mean cord blood serum magnesium level in control and study group was 0.85±0.12 and 0.83±0.10. The serum magnesium levels at 12, 24, 48 and 72 hours were 1.80±0.16, 1.82±0.16, 1.81±0.18, 1.82±0.17 in the study group, which were significantly higher than those in control group. The serum magnesium levels in study group ranged between 1.50 mmol/L & 2.20 mmol/L which are considered to be neuroprotective range. No significant alteration in vital parameters was seen following magnesium infusion. Evidence of HIE was seen more frequently in the control group with 26 (65%) babies showing HIE versus 22 (55%) in the study group. The incidence of HIE, neonatal seizures, & apnoeic spells were lesser in the study group compared to control group. Conclusions: Injection MgSO4 administered in a dose of 250 mg/kg & 125mg/kg as intravenous infusion is safe, Mg level obtained are in neuroprotective range & it causes better immediate outcome.

Assessment of Important Trace Elements in Jordanian Adult Females and Males by Using Atomic Absorption Spectrophotometer

2 Abstract: The human body requires certain minerals for the overall health and proper functioning of th e organ systems. The essential trace elements were very important for human life, which their imbalance in blood leads to very dangerous diseases (Zn, Cu, Fe, Cr, Se, Co, Mn and Mg). The purpose of this study was to evaluate the concentrations of Zinc, Copper, Iron, Magnesium, Chromium, Selenium, Cobalt and Manganese in serum of healthy adult females and males in Jordan. Serum levels of Zn, Cu, Fe, Mg, Cr, Se, Co, Mn and Mg were determined by flame and flameless atomic absorption spectrophotometer. Blood have been collected from1000 men and 1000 women apparently healthy Jordanian people aged 20-70 years. The mean concentration of zinc was between (0.96-1.02 and 0.8-1.02 µg/mL) in men and women, respectively. The concentration of zinc was slightly increased with ages. The mean values of copper were between (1.38- 1.72 µg/mL), there is no significance deficiency in all ages and sexes. The average mean values of Fe were found to be (1.28-1.62 µg/mL) for men and (1.12-1.57 µg/mL) for women, with a deficiency of 5.9% in men and 8.5% in women. The chromium mean in men were between (0.04-0.055 µg/mL) with a deficiency of 43.06%, while in women were between (0.041-0.049 µg/mL), with a deficiency of 40.28%. The concentration of magnesium in blood serum was between 14.2-16.1 µg/mL for men and 14.3-16.3 µg/mL for women. The mean selenium concentrations in all healthy volunteers were in the range of 85-100 ng/mL for men and 82- 99 ng/ml for women. The lowest levels of Se were shown in old people. The adult serum Co values were 0- 34 ng/mL for men and 0-35 ng/mL for women. The cobalt deficiency was about 17% in men and women. The level of serum Manganese was 14-19 ng/mL for men and 11-15 ng/mL for women, it was noted that the level of Mn in men was higher than in women for all ages. The Mg concentrations of serum were 14.10±3.3 and 14.89±3.3 µg/mL for men and women. On the basis of this study finding and those of other reports, trace elements deficiency may contribute to diets and diseases through its significant effects on various organs parameters. It seems that the estimation of serum or plasma trace elements may help in investigation and treatment of some diseases in both males and females.

Variation of Copper, Zinc, Manganese and Magnesium in Blood Serum and Tissues of Two Breeds of Dromedary Camels in Saudi Arabia

Variation of Copper, Zinc, Manganese and Magnesium in Blood Serum and Tissues of Two Breeds of Dromedary Camels in Saudi Arabia

Relationship Of Connective Tissue Dysplasia And Hypomagnesemia In Genesis Of Juvenile Dysmenorrhea

It was studied the relationship of connective tissue state with magnesium and hydroxyproline levels in blood and urine of young women with primary dysmenorrhea. Study showed that in girls with dysmenorrhea and phenomena of connective tissue dysplasia hydroxyproline level in urine was increased which was associated with increased degradation of collagen and decreased level of magnesium in blood serum. This should be noted in differentiated approach to the treatment of dysmenorrhea.

The consequences of using mixture with buffers effects on some biochemical parameters in blood serum during the fattening of the lambs

This work has an aim to explore the effects of the product based on the natural zeolit during the fattening of the lambs and also the effects on contents of calcium, phosphorus and magnesium in blood serum. The experiment lasted 15 days in the closed area of the Agricultural school in Kraljevo, and the lambs were divided into three groups of 15 lambs (Control group -C, Experimental group E1 and E2). They were fed with sheep?s milk, concentrate for lambs? fattening and meadow hay. The lambs in the experimental groups, in contrast to Control group, were fed with the different concentration of the preparation based on the natural zeolit (O1=1%, O2=1.5%), so that the manifested differences would be treated as the resault of the different concentration of the zeolit that was added to the lambs? food.At the end of the experiment the differences in the parameters of the lambs? blood serum were distinguished and especially in the concentration of calcium, phosphorus and magnesium.

Serum magnesium concentration in drug-addicted patients.

Drug addiction is a complex problem which leads to many somatic, psychic and social diseases. It is accompanied by the disturbed metabolism of various macro and micronutrients. The aim of this study was to assess serum magnesium concentration in drug-addicted patients and analyze whether Human Immunodeficiency Virus (HIV) infection and methadone treatment affect the level of serum magnesium in these patients. The examination was conducted in a group of 83 people - patients of Szczecin-Zdroje Psychiatric Hospital (Poland). They were 21 to 49 years old, and the mean age was 32 +/- 7 years. The control group consisted of 81 healthy individuals. Flame atomic-absorption spectrometry method was used to determine the magnesium concentration. The total serum magnesium concentration was calculated for the whole patient group, subgroups of women and men, a subgroup of people infected with HIV, and a subgroup receiving methadone substitution treatment. How magnesium behaves depending on age and addiction period, was checked. The mean concentration of magnesium in blood serum of the patients examined was 0.57 mmol/L, which was significantly lower than in the control group. In the subgroup of men it was 0.57 mmol/L, and in the subgroup of women - 0.55 mmol/L; the differences were not statistically significant. In the patient group nobody had the appropriate magnesium concentration in blood serum. No significant correlation was found between the magnesium concentration, age of the patients and addiction period. In the subgroup of seropositive people the mean concentration of magnesium was 0.55 mmol/L, and in the subgroup of non-infected patients - 0.58 mmol/L; the difference was not statistically significant. The mean concentration of magnesium in the subgroup treated with methadone was 0.59 mmol/L, and in the subgroup not involved in this type of therapy - 0.55 mmol/L; it was not a statistically significant difference.

Spectrofluorimetric determination of magnesium (II) with 7-(8-Hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid

A new fluorescent reagent, 7-(8-hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex withλex/λem immediately at room temperature in ammonia-ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0–160 ng·mL−1 with the detection limit of 0.04 ng·mL−1. The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively.