Madhuca Indica Research Articles

A study on bacterial inactivation using green synthesized photocatalytically active TiO2, MgO, TiO2/MgO nanoparticles

Madhuca longifolia (Mahua) flower extracts were utilised to perform the green synthesis of titanium dioxide (TiO2), magnesium oxide (MgO) nanoparticles, and a nanocomposite consisting of TiO2/MgO. Mahua flower extracts were used as both reducing and capping agents in a sol-gel synthesis method. The effectiveness of the green reducing agent in improving the surface structure of the green nanoparticles was demonstrated. FTIR, XRD, FESEM, and UV vis spectroscopy were employed to analyse the successful synthesis of TiO2, MgO, and TiO2/MgO nanocomposites. A study was done to examine the process of employing these nanoparticles to deactivate Escherichia coli (E. coli) by photocatalysis. The effectiveness of these nanoparticles in inhibiting the growth of the bacterial species was demonstrated. The optimisation of the bacterial inactivation experiment was performed using Design Expert's RSM CCD model on all three nanoparticle samples. The optimisation study investigated the impact of nanoparticle concentration and exposure duration on the inhibition of bacterial growth. The highest levels of percent inhibition (%I) were recorded as 85.4 % for TiO2/MgO, 82.8 % for TiO2, and 65.14 % for MgO at a concentration of 50 mg/2 ml and an exposure time of 2 h. The effectiveness of these nanoparticles in exhibiting inhibition has been limited, indicating their prospective use in the advancement of antimicrobial coatings and other applications, such as disinfectants, in the future.

Climatic Oscillations and Dynastic Trends: A Multiproxy analysis of the past two millennia in the Indian Subcontinent

The study examines centennial-scale climatic oscillations over the past two millennia in the monsoon-dominated Indian subcontinent by multiproxy analysis of pollen along with stable carbon isotope, total organic carbon, magnetic susceptibility, and grain size from a 274 cm deep sediment profile in Sarsapukhra Lake, Varanasi, in the Central Ganga Plain (CGP) to portray vegetation changes and their impact on rise and fall of dominant dynasties. Between ∼1800 to 1600 cal yr BP, the coarser silty sand with few Poaceae and Asteraceae pollen with other proxy data, indicate that the region experienced a strong Indian Summer Monsoon (ISM). Subsequently, from ∼1600 to 1350 cal yr BP, presence of mixed moist deciduous taxa such as Shorea robusta, Madhuca indica, Rubiaceae, Morus alba, Syzygium, and Moraceae indicate a moderate ISM while between 1350 to 1200 cal yr BP, the dominance of dry deciduous taxa viz. Acacia sp., Bombax ceiba, Holoptelea, Rutaceae, Madhuca indica, Mangifera indica etc. suggested weak ISM conditions. A pulsative ameliorating trend from ∼1200 to 1030 cal yr BP showed the dominance of deciduous taxa such as Holoptelea, Rutaceae, Ziziphus, Rubiaceae, Maytenus and Acanthaceae etc. indicating warm and moist climatic conditions, followed by a weak ISM until ∼900 cal yr BP and improved ISM until 620 cal yr BP. Subsequently, an improving trend in the ISM from ∼620 to 450 cal yr BP was observed, with increased dominance of wetland taxa, followed by acute dry climate beyond ∼450 cal yr BP indicating poor ISM conditions.Additionally, the alternating warm and cold episodes had a direct impact on vegetation, forcing human civilizations to migrate from their original locations and contributing to the downfall of prominent dynasties. Furthermore, this study lays the foundation for future research into issues related to vegetation change, the amount of ISM shift, and human culture−climate relationship.

Effect of hybrid nanoparticles dispersed ternary fuel blend on diesel engine performance and emissions: Experimental and Taguchi-Grey relation study

The present study deals with the influence of hybrid nanoparticles dispersed in ternary fuel mixtures on the evaluation of the performance and emission characteristics of a single cylinder diesel engine. The fuel blend consists of 70% diesel, 20% Madhuca longifolia biodiesel, and 10% ethanol (by volume). The hybrid nanoparticles of ferric chloride (FeCl3) and graphene nanoparticles were then added to this ternary fuel mixture in different proportions, namely 50 mg/L and 75 mg/L, together with the surfactant and the dispersant in a ratio of 1:1. Moreover, the experiments were conducted on a diesel engine to evaluate the performance and emission parameters by varying different fuel samples, different loads (3, 6, 9, and 12 kgf) and injection pressures (i.e. 200, 225,and 250 bar). In addition, the Taguchi-Grey relation analysis was used for optimization, and the input parameters are fuel samples, loads and injection pressures. Experimental performance metrics, including brake specific fuel consumption (BSFC) and brake thermal efficiency (BTE), were improved when hybrid nanoparticles were added to the ternary fuel blend compared to diesel. At the same time, smoke opacity, nitrogen oxides (NOx), unburned hydrocarbons (UHC) and carbon monoxide (CO) emissions were reduced. Increasing the injection pressure also led to positive results, apart from NOx. Both performance and emissions were better for hybrid nanofuel based on surfactants and dispersants than for the base fuel, and it performed better of all dispersant-added nanofuels. The better results were obtained with D70B20E10NPs75QPAN75 mg/L than with the other fuel samples. The maximum BTE and lowest BSFC are 33.26% and 0.206 kg/kWh, while the minimized CO, UHC, NOx and smoke values at full load are 0.019%, 21 ppm, 833 ppm and 36.25%, respectively, at full load and injection pressure of 250 bar. By combining experimental investigations with the Taguchi -Grey method, a comprehensive and rapid assessment of the effects on the performance and emission characteristics of diesel engines was achieved.

Phytochemical Extraction, Analysis, and Characterization of Madhuca longifolia Leaf Compounds.

Objective: This study aimed to perform the extraction, phytochemical analysis, and compound characterization of Madhuca longifolia leaf extracts. Materials and Methods: Sequential extraction of M. longifolia leaves was performed using petroleum ether, chloroform, methanol, ethanol, and water. The yield of the extracts was measured and reported as 1.3 ± 0.10% for petroleum ether, 3.85 ± 0.15% for chloroform, 20.25 ± 1.22% for methanol, 30.1 ± 1.38% for ethanol, and 26.7 ± 0.21% for water. Qualitative phytochemical analysis was conducted to determine the presence of various compounds, including alkaloids, glucose-alkaloids, steroids, tannins, phenols, proteins, and flavonoids. The absence of ethyl and methyl alkalis in petroleum ether and chloroform extracts was noted. Additionally, the presence of saponins and alkaloids was observed only in methanol extracts, with cresol absent from these extracts. The isolated compounds were further characterized using thin layer chromatography (TLC), highperformance liquid chromatography (HPLC), ultraviolet (UV) spectroscopy, and Fourier-transform infrared (FTIR) spectroscopy. Results: The Rf value of the isolated compound was 0.49 using TLC. HPLC analysis identified major constituents with retention times of 4.576 and 10.461 minutes. These findings highlight the diverse phytochemical composition of M. longifolia leaf extracts and provide a detailed analysis of the active compounds present. Conclusion: This comprehensive study of M. longifolia leaf extracts offers valuable insights into the plant’s medicinal potential. The identified active compounds could serve as a reference for future medicinal use and research.

High-quality chromosome-level genomic insights into molecular adaptation to low-temperature stress in Madhuca longifolia in southern subtropical China

BackgroundMadhuca longifolia, the energy-producing and medicinal tropical tree originally from southern India, faces difficulties in adapting to the low temperatures of late autumn and early winter in subtropical southern China, impacting its usability. Therefore, understanding the molecular mechanisms controlling the ability of this species to adapt to environmental challenges is essential for optimising horticulture efforts. Accordingly, this study aimed to elucidate the molecular responses of M. longifolia to low-temperature stress through genomic and transcriptomic analyses to inform strategies for its effective cultivation and utilisation in colder climates.ResultsHerein, the high-quality reference genome and genomic assembly for M. longifolia are presented for the first time. Using Illumina sequencing, Hi-C technology, and PacBio HiFi sequencing, we assembled a chromosome-level genome approximately 737.92 Mb in size, investigated its genomic features, and conducted an evolutionary analysis of the genus Madhuca. Additionally, using transcriptome sequencing, we identified 17,941 differentially expressed genes related to low-temperature response. Through bioinformatics analysis of the WRKY gene family, 15 genes crucial for M. longifolia low-temperature resistance were identified.ConclusionsThis research not only lays the groundwork for the successful ecological adaptation and cultivation of M. longifolia in China’s southern subtropical regions but also offers valuable insights for the genetic enhancement of cold tolerance in tropical species, contributing to their sustainable horticulture and broader industrial, medicinal, and agricultural use.

Study on sensory evaluation crushed dried mahua (Madhuca longifolia) flowers in the preparation of Kulfi

Study on sensory evaluation crushed dried mahua (Madhuca longifolia) flowers in the preparation of Kulfi

Examining spatial and social characteristics of customary trees owned by villagers on public forestlands in Jharkhand, India

Tree ownership is typically tied to land ownership in many societies worldwide. Still, in India, tribal communities have customary ownership over trees in public forestlands, raising questions about what those trees are, where they are located, their characteristics, and whether socioeconomic factors influence their distribution and density. To address these questions, we conducted household interviews and a Global Positioning System (GPS) mapping in Sahritola, a village in the State of Jharkhand, India. We employed Kruskal Wallis, Spearman correlation, and Moran's Index to analyze the collected data. The villagers have been protecting and managing over 18,000 trees in public forestlands, including species such as asan (Terminalia tomentosa), sal (Shorea robusta), and mahua (Madhuca longifolia). Over 80 % of those trees are under individual ownership, while around 20 % are under shared ownership. Additionally, over 60 % of households owned between 100 and 500 trees. Customarily-owned tree species sharing similar characteristics (e.g., height, crown width, and diameter-at-breast-height) tend to cluster together. The number of customarily owned trees is influenced by their economic value and socioeconomic factors (e.g., religion, forestland, etc.). Additionally, the type of customary ownership (individual or shared) influences the characteristics of trees. This study could guide policymakers in potentially developing forest policies that consider the concept of customary tree ownership to promote sustainable management of forest resources in India and beyond.

An integrated biorefinery of Madhuca indica for co-production of biodiesel, bio-oil, and biochar: Towards a sustainable circular bioeconomy

The advancement of non-waste biorefinery technology has proved beneficial in effectively using waste biomass resources. The potential of various biorefinery designs to transform biomass into a range of commercially viable products and bioenergies has piqued attention worldwide among the several scenarios proposed to commercialize biofuels. This paper describes the biorefinery of Madhuca indica (M. indica) seeds to generate biochar, bio-oil, and biodiesel. Pyrolization was used on the de-oiled cake to produce charcoal and bio-oil. The M. indica seed's biochar was utilized as a useful supporting particle to immobilize the enzyme. Through transesterification, the immobilized enzyme transformed the oil extracted from the seed into biodiesel. Using an optimum methanol-to-oil ratio of 7.5:1, 3 % (w/w) of catalyst, and 16 % (v/v) of water content, the method yielded a maximum fatty acid methyl ester (FAME) conversion of 93.4 %. Diesel and 20 % biodiesel have been assessed in terms of performance and emissions analysis. Comparing a 20 % blend of biodiesel to commercial diesel at full load, the emissions of carbon monoxide (CO) were reduced by 21.2 %, hydrocarbon (HC) emissions by 18.02 %, and nitric oxide (NO) emissions by 4.33 %. In addition, the capital flow of the suggested biorefinery was assessed using a techno-economic analysis. The biorefinery process payback period for the biodiesel, bio-oil, and charcoal made from M. indica was determined to be 3.1358 years using the Aspen Plus Economic Analyzer. Techno-economic studies reveal that the biorefinery process is highly lucrative and practical. The current study therefore shows methods to use the Madhuca indica seed biorefinery to produce sustainable energy and byproducts with no net emissions of carbon.

Mahua (Madhuca indica): indigenous flower as nutrient and phenolic rich food to combat anaemia and associated disorders

Mahua (Madhuca indica): indigenous flower as nutrient and phenolic rich food to combat anaemia and associated disorders

The Madhuca longifolia: A multifaceted tree with rich in nutritional factors

The Madhuca longifolia: A multifaceted tree with rich in nutritional factors

Updates on some medicinal and ornamental plants- Ayurvedic medicines

This literature review paper highlights morphology, distribution, medicinal properties of commercially important tree species, Thespesia populnea, Terminalia arjuna, Butea monosperma (Palas), Silver oak (Grevillea robusta), Mangifera indica, Madhuca longifolia,Melia azadirachta, Michelia champaca, Moringa oleifera, Peltophorum pterocarpum, and Aegle marmelos. These plants have been used for centuries by local communities and traditional healers for their therapeutic and medicinal properties. Medicinal plants play a crucial role in the Western Ghats, both ecologically and culturally. The use of herbal medicines for the treatment of diseases is safe and traditional. Therefore, medicinal plants have been receiving great attention because of their safety. The Western Ghats harbors more than 7,500 flowering plant species, of which nearly 1,500 are recognized for their medicinal values by local communities and traditional healers. The Western Ghats population traditional medical practices frequently center on using plant-based treatments. These medicinal plants are carefully chosen, and preparation techniques have been perfected through many years of practice. Studies indicated that Mangifera indica possesses antidiabetic, anti-oxidant, anti-viral, cardiotonic, hypotensive, and anti-inflammatory properties. Mango contains Mangiferin, a polyphenolic antioxidant and a glucosyl xanthone, has strong antioxidant, anti lipid peroxidation, immunomodulation, cardiotonic, hypotensive, wound healing, antidegenerative and antidiabetic activities.

Documentation On Medicinal Plants of Khanapur Forest Bidar District, Karnataka

The present study was undertaken to document medicinal plants of Khanapur forest Bidar district, karnataka the documentation of medicinal plants helps to recognize the overall ecological conditions and gives profound understanding of the economic medicinal and traditional importance of medicinal plants diversity. The present research documentation work was started in November 2023 and completed in February 2024. The present study reported that 119 Medicinal plant species belongs to 54 families distributed in Khanapur forest Bidar included angiosperms Pteridophytes and Gymnosperms. Abrus precatorius, Ephedra, Withania sominifera, Catharanthus roseus, Tinospora cardifolia, Andrographis paniculata, Phyllanthus amarus, allium sativum, Cocos nucifera, Coleus aromaticus, Curcuma longa, Martynia annua, Ocimum tenuiflorum, Tamarindus indica, Terminalia chebula,Tithonia diversifolia, Acalypha indica, Brassica juncea, Clitoria ternatea, Datura metal, Delonix elata, Euphorbia antiquorum, Luffa acutangula, Madhuca longifolia, Myristica fragrans, Santalum album and Vitex negundo are some of the important medicinal plants of Khanapur forest Bidar district of karnataka.

Rationalization of post-harvesting unit operation of mahua flowers (Madhuca longifolia, Madhuca indiaca): Systematic interventions and benefits for tribals

<p>The most common method used for collection of mahua flowers by burning the undergrowth under mahua trees which often caused forest fire and loss or flora and fauna of nature. The comparative analysis of traditional flower collection vis-a-vis net collection was analyzed during the research. The most significant benefits were on prices, time reduction and quality of products. These benefits will encourage the adoption of this technique by forest dwelling communities for mahua collection. The net collected flowers sold at 110 to 120 ₹ compared with 25–30 ₹ with traditional collection (<em>p</em> < 5 ₹). The time and manpower reduced by 80% (<em>p</em> < 1%) in net collection. Total 87.95 K MT mahua exported from India with value of 70.01 million USD in FY 2022. India rank 7th in global export of Mahua in 2022. Nearly 75% of entire tribal household in the country are involved in the collection of mahua flower which will contributes up to 30% of their annual cash income. To handle mahua around 11 lakh peoples are directly involved and 20 to 25 lakh peoples indirectly involved. To produce liquid >90% mahua utilized in India. During this research post-harvest unit operations also standardized. New food product like syrup, cookies, jam, spread, jelly, gummies, energy drink, beverages, hard-boiled candies development were done, and stabilities were conducted with utilization of 10% to 60% mahua.</p>

From ban to brand—Changing scenario of Mahua (Madhuca longifolia, Madhuca indica) in India and become the fulcrum for tribal communities

<p>Mahua is undergoing a rebranding, but India needs legislation to safeguard tribal rights. Mahua is entwined with the tribal world, yet incorrect colonial ideas about it as a toxic, lowly beverage still plague it. Mahua has a close relationship with Gond, Santhal, Baiga, Munda, Ho, Orao, and all other communities dispersed across at least 12 states. For instance, the social rites of Tonda (birth), Manda (marriage), and Konda (death) for a Gondi Adivasi would end in the absence of mahua. Mahua brewing was merely one more duty in an indigenous family’s home until the late 1800s. The British Raj considered this to be an unexplored source of income. With a series of laws, including the Bombay Abkari Act of 1878, the Mhowra Act of 1878, 1892, the Madhya Pradesh Excise Act of 1915, the Bihar and Orissa Excise Act of 1915, and the Government of India Act of 1935, they outlawed not only the production of mahua but also the gathering and storing of mahua flowers. They considered it a low-quality toxicant and labelled it as a threat to public health and morality. As a result, they could regulate the production of regional spirits and made money from the importation of alcohol from Germany and Britain. Following India’s independence, regulations changed but not entirely. Mahua and tribal people were affected by laws such as the Bombay Mahua Flower Rules of 1950, the Bihar Prohibition and Excise Act of 2016, the PESA of 1996, the Forest Right Act of 2006, and total prohibition, which included mahua distillation, which was enforced in Gujarat and Wardha in 1960. The average family in the Indian states of Maharashtra, Madhya Pradesh, and Jharkhand will make 10.5 K, 16.4 K, and 15.75 K ₹ annually. Mahua is included in the minimum Support Price by the Indian government. Mahua has an estimated potential production of 1.5 lakh MTs, valued at 122 ₹ Cr, in India; however, only 1.0 lakh MTs, valued at 81 ₹ Cr, are expected to be collected annually. Though there is a 1.63 lakh job potential, just 28.6 K, or 17.5%, of Indians are employed. Mahua appears to be gradually reclaiming its space after going extinct for decades. The British Raj previously outlawed mahua; today, it is one of India’s most prestigious brands, sold all over the world.</p>

Network Pharmacology Reveals Key Targets and Pathways of Madhuca longifolia for Potential Alzheimer's Disease Treatment.

Madhuca longifolia, commonly known as the mahua tree, has been traditionally used in medicine due to its anti-inflammatory, anti-diabetic, and antimicrobial properties. Its active compounds help in managing diabetes, alleviating cognitive impairment associated with Alzheimer's disease. Nonetheless, the exact neuroprotective mechanism of Madhuca longifolia against Alzheimer's disease remains unclear. This study looked into possible methods by which Madhuca longifolia protects against Alzheimer's disease using network pharmacology, molecular docking and molecular dynamic simulations studies. By applying pre-screening of active constituents, target prediction, Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis, our study found that Madhuca longifolia is related to eight active ingredients (Ascorbic acid, Riboflavin, Pantothenic acid, (4 R)-2beta,3beta,23-trihydroxy-oleana-5,12-dien-28-oic acid, Quercetin, Nicotinic acid, Bassiaic acid Thiamine) and 272 common gene targets, with significant involvement in pathways such as PI3K-Akt signaling and neuroactive ligand-receptor interaction. Network analysis demonstrated how Madhuca longifolia can prevent AD by modifying important signalling networks, which may be one of the molecular mechanisms driving the plant's effectiveness against the disease. Molecular docking studies revealed that there were robust binding abilities of Quercetin, Riboflavin and Pantothenic acid to key target proteins AKT1, JUN, and STAT3. Later, molecular dynamic simulations was done to examine the successful activity of the active compounds against potential targets, and it was found that AKT1 and AKT1-Quercetin complex became stable at 260 ps. It may be seen through the study that quercetin may act as a good inhibitor for treatment. This thorough investigation provides a strong basis for future research and development efforts by advancing our understanding of Madhuca longifolia medicinal potential in Alzheimer's disease.

How can dry tropical forests respond to climate change? Predictions for key Non-Timber Forest Product species show different trends in India.

The present study provides an assessment of the distribution of key Non-Timber Forest Product species in India, namely Aegle marmelos (L.) Correa, Buchanania lanzan Spreng., Madhuca longifolia (J. Koenig ex L.) J. F. Macbr., Phyllanthus emblica L. and Terminalia bellirica (Gaertn.) Roxb. The suitable habitat was analyzed under current climate scenarios and subsequently, the future distribution (2050s and 2070s) was mapped under RCP 2.6 and 8.5 scenarios, along with the past distribution (mid-Holocene, ~ 6000calyear BP) using the MaxEnt species distribution model. The distribution of all species is primarily driven by key bioclimatic factors, including annual precipitation (Bio_12), mean annual temperature (Bio_1), isothermality (Bio_3) and precipitation of the coldest quarter (Bio_19). The results indicate that the present distribution of these species is mainly centred in the Western Ghats regions, Central Highlands, North-eastern India and Siwalik hills. The current study suggests that under the future climate change, the suitable habitat for A. marmelos and T. bellirica is expected to increase while for B. lanzan, M. longifolia and P. emblica, it is projected to decline. A. marmelos and T. bellirica are anticipated to exhibit resilience to future climate changes and are expected to be minimally affected, while B. lanzan, M. longifolia and P. emblica are highly sensitive to high temperature and alteration in rainfall pattern expected under future climate changes. The projections of habitat suitability areas can be used as a valuable foundation for developing conservation and restoration strategies aimed at alleviating the climate change impacts on NTFP species.

Documentation On Medicinal Plants of Deva Deva Vana Botanical Garden of Bidar District, Karnataka

The present study was undertaken to document medicinal plants of Deva Deva vana Botanical garden of Bidar district, karnataka. Deva Deva vana literally means ‘Forest of divine/celestial beings’ A Botanical garden maintained by the Forest department around 6 km from Bidar – Hyderabad Highway. It is home to around 200 medicinal plants and trees, and considered sacred. It is divided into around 10 gardens and a nursery which is classified according to a particular theme like Nandana vana, Ashoka vana, Navagraha vana, Rock Garden, shiva panchayath vana, Phala pushpa vana,..etc the documentation of medicinal plants helps to recognize the overall ecological conditions, conservation and gives profound understanding of the economic medicinal and traditional importance of medicinal plants diversity. The present research documentation work was started in February 2023 and completed in June 2024. The present study reported that 147 Medicinal plant species belongs to 64 families distributed and conserved in Deva Deva vana Botanical garden Bidar included angiosperms Pteridophytes and Gymnosperms. Abrus precatorius, Ephedra, Withania sominifera, Catharanthus roseus, Tinospora cardifolia, Andrographis paniculata, Phyllanthus amarus, allium sativum, Cocos nucifera, Coleus aromaticus, Curcuma longa, Martynia annua, Ocimum tenuiflorum, Tamarindus indica, Terminalia chebula,Tithonia diversifolia, Acalypha indica, Brassica juncea, Clitoria ternatea, Datura metal, Delonix elata, Euphorbia antiquorum, Luffa acutangula, Madhuca longifolia, Myristica fragrans, Santalum album and Vitex negundo are some of the important medicinal plants of Deva Deva vana Botanical garden Bidar district of karnataka.

Variability in Seed Morphology, Germination, and Seedling Growth of Madhuca indica: Implications for Seed Source Selection

Variability in Seed Morphology, Germination, and Seedling Growth of Madhuca indica: Implications for Seed Source Selection

Pretreatment of Melanoma Cells with Aqueous Ethanol Extract from Madhuca longifolia Bark Strongly Potentiates the Activity of a Low Dose of Dacarbazine.

Madhuca longifolia is an evergreen tree distributed in India, Nepal, and Sri Lanka. This tree is commonly known as Mahua and is used in traditional medicine. It was demonstrated that ethanol extract from the bark of M. longifolia possessed potent cytotoxic activity towards two melanoma cell lines, in contrast to aqueous extract that exhibited no activity. Apart from being selectively cytotoxic to cancer cells (with no activity towards non-cancerous fibroblasts), the studied extract induced apoptosis and increased reactive oxygen species generation in melanoma cells. Additionally, the use of the extract together with dacarbazine (both in non-toxic concentrations) resulted in the enhancement of their anticancer activity. Moreover, the pretreatment of melanoma cells with M. longifolia extract potentiated the activity of a low dose of dacarbazine to an even higher extent. It was concluded that ethanol extract of M. longifolia sensitized human melanoma cells to chemotherapeutic drugs. It can therefore be interesting as a promising source of compounds for prospective combination therapy.

Restoring a dry tropical forest through assisted natural regeneration: enhancing tree diversity, structure, and carbon stock

Deforestation is a key factor in global climate change, severely impacting ecosystem services. Effective implementation of reducing emissions from deforestation and forest degradation (REDD+) initiatives is essential for mitigating this change. Reforestation is a sustainable way to mitigate deforestation effects. We studied the impact of assisted natural regeneration (ANR) on restoring dry forest land, increasing biodiversity, structure, and carbon stock. Reforestation units were established at a multiple row planting system in a dry tropic region. We selected 63 plots, each 20 m × 20 m, categorized into multiple row–mixed plantation (MRMP) and multiple row–unmixed plantation (MRUP). We measured tree height and diameter at breast height (DBH) and evaluated biodiversity indices, including the Shannon–Weiner Index (SWI), species richness, evenness, and Important Value Index (IVI). Carbon stock was estimated using allometric equations. We identified 931 individual trees representing 27 species, 24 genera, and 14 families. MRMP showed significantly higher biodiversity, species richness, and evenness than MRUP. Terminalia arjuna was the most dominant species (IVI=124.45), followed by Khaya senegalensis (53.84). Survival rates exceeded 90 % for T. arjuna, K. senegalensis, Madhuca longifolia, and Pongamia pinnata (p<0.001). No significant differences were observed in tree DBH, tree height, tree density, basal area, and carbon stock among the plantation categories. The species Albizia saman exhibited a high diameter increment rate (4.07 ± 1.55 cm/year), followed by K. senegalensis (3.83 ± 0.43 cm/year). A mean value of 5.63 ± 1.13 MgC/ha of carbon stock was stored, while 20.66 ± 4.13 Mg/ha of atmospheric CO2 was sequestered. The results highlight that promoting mixed tree species in multiple rows to enhance biodiversity and optimize carbon regulation. This technique should be popularized to restore the dry forest landscape across the region.