Macroporous Adsorption Resin Research Articles

Determination of absolute configuration of a new triterpenic acid in leaves of Ilex hainanensis

This study aimed to explore the triterpenic acid components in leaves of Ilex hainanensis. Alkaline water extraction, macroporous resin adsorption, and high performance liquid chromatography were used to separate and purify the triterpenic acid components in leaves of I. hainanensis. The physical and chemical property analysis, MS, NMR spectroscopy, and literature comparison were performed to identify the structures, and a new triterpene acid compound was discovered:(3S, 4R, 5R, 8R, 9R, 10R, 14S, 17S, 18S, 19R)-3,19-dihydroxyursa-12,20(30)-diene-24,28-dioic-acid, and named ilexhainanin F. In addition, according to its structural characteristics, the ~(19)F-NMR Mosher method was further employed to study its absolute configuration. By comparison of the ~(19)F-NMR chemical shifts of Mosher esters, it was determined that the absolute configuration of the 3-position chiral center of the compound was the S configuration.

Static decolorization of polysaccharides from the leaves of Rhododendron dauricum: Process optimization, characterization and antioxidant activities

In this study, polysaccharides from the leaves of Rhododendron dauricum L. (RDPs) were decolorized using the static macroporous resin adsorption method. The RDPs were characterized and their antioxidant activities were investigated. An analytical hierarchy process (AHP) and a response surface method (RSM) were used to determine the optimum decolorization conditions as: a polysaccharide concentration of 8 mg/mL, decolorization time of 70 min, liquid–solid ratio of 6 mL/g, and decolorization temperature of 52 °C, with D101 as the best resin. Furthermore, two novel polysaccharides (RDP-1 and RDP-2) were isolated from R. dauricum leaves and characterized, which demonstrated that RDP-1 and RDP-2 were both composed of mannose, glucose, galactose, and arabinose in different molar ratios. These RDPs also exhibited a triple helix conformation, and primarily contained →6)-Glcp-(1→, →5)-Araf-(1→, →2,4)-Manp-(1→, →6)-Galp-(1→ and Glcp-(1→ residues with different connection sequences. Biological activity tests showed that the two polysaccharides possessed scavenging capacity against 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radicals. The results indicated that the antioxidant activities of the RDPs obtained by the static decolorization method improved, which lays a theoretical foundation for developing techniques for utilising R. dauricum.

Isolation and characterization of water-soluble fractions of black sesame pigment and its antioxidant activities in vitro

ObjectiveBlack sesame pigment (BSP) was a natural pigment with a variety of bioactivities, including antioxidant, antimutagenic, and neuroprotective properties. However, the high molecular weight and heterogeneous structure of BSP necessitate further investigation. The study's goal is to develop a theoretical foundation for water-soluble BSP as a potential functional food supplement. MethodsFirst, crude black sesame pigment (rBSP) was extracted from black sesame by sodium hydroxide-hydrochloric acid method, furtherly two water-soluble components (BSP-1, BSP-2) were prepared from rBSP by the separation methods of macroporous adsorption resin. In the dynamic test, 40% ethanol (v/v) was applied to five different macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) for static adsorption/desorption of rBSP separation. The structure of the components was investigated using ultraviolet (UV) spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), and 1H and 13C nuclear magnetic resonance (NMR). Oxygen radical absorption capacity (ORAC) and cellular antioxidation activities (CAA) were used to assess the antioxidant activity of the components. ResultsA macroporous polymeric adsorbent method was developed to obtain water-soluble fractions of rBSP. For the static adsorption/desorption characteristics of rBSP, five types of macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) were evaluated, and two fractions (BSP-1 and BSP-2) were isolated from rBSP by 40% ethanol (v/v) on D101 resin in the dynamic test. Furthermore, ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, 1H and 13C NMR revealed that BSP-1 and BSP-2 were macromolecule conjugate structures, with BSP-2 having higher aromaticity than BSP-1. ORAC and CAA results showed that rBSP, BSP-1, and BSP-2 had comparable antioxidant activities. The BSP-2 exhibited the highest CAA value of 1121.92 ± 54.45 µmol QE/100 g, DW. ConclusionThis study suggests that water-soluble BSP fractions could be used as functional food supplements that benefit human health.

Electrochemical-Based Extraction, Separation, and Purification of Coumarin Compounds from Trifolium chinensis

In order to deal with the problems of the unstable transformation process and volatile palatability of coumarin compounds in C. chinensis, a method for the extraction, separation, and purification of coumarins in Chinese medicinal materials based on electrochemistry was prepared. First, an electrochemical distance-independent localization mechanism is used to determine node locations and routing mechanisms, and an extraction method is designed to achieve automatic and accurate real-time collection, aggregation, and transmission of ever-growing data. Environmental information for C. chinensis is collected. On the basis of this method, according to the chemical properties of coumarin and the extraction rate of coumarin as an index, the two-phase solvent extraction method and macroporous adsorption resin method were used to separate and purify it, and the best separation was determined. The purification process achieves efficient purification of coumarin. The experimental results show that the retention rate of coumarin by ethyl acetate is 34.5–56.8% higher than that of the other three extractants. When the optimal process determined in this study is adopted, the coumarin adsorption rate is greater than 98% and the recovery rate is greater than 85%. The purity of samples with 50% coumarin content can be increased to more than 97%, which verifies the correctness and advancement of the separation and purification process in this study.

An effective method for preventing cholestatic liver injury of Aucklandiae Radix and Vladimiriae Radix: Inflammation suppression and regulate the expression of bile acid receptors

Ethnopharmacological relevanceAucklandiae Radix (AR) and Vladimiriae Radix (VR) were used to treat gastrointestinal, liver and gallbladder diseases at practice. In most conditions, VR was used to be a substitute of AR or a local habit may attribute to the same main active ingredients Costunolide and Dehydrocostus lactone, which presented many similar pharmacological activities. However, other different lactone compounds in AR and VR also play a role in disease treatment, so the difference in therapeutic effects of AR and VR in related diseases needs to be further studied. Aims of the studyRevealing the differences between the chemical compounds of the total lactone extracts of AR and VR (TLE of AR and VR) and the differences in the protective effects of cholestatic liver injury to ensure rational use of AR and VR. Study design and methodsThe macroporous adsorption resin was used to purify and enrich the lactone compounds to obtain the total lactone extracts of AR and VR. HPLC-PDA was used to obtain the data to establish chemical fingerprint and chemometric analysis to compare similarities and differences between TLE of AR and VR. The pharmacodynamic experiment revealed how TLE of AR and VR to show protect effects on cholestatic liver injury. ResultsSimilarity analysis results showed TLE of AR and VR had a high similarity (>0.9). Nevertheless, difference analysis results showed 4 compounds, Costunolide, Dehydrocostus lactone, 3β-acetoxy-11β-guaia-4 (15), 10 (14)-diene-12,6α-olide and vladinol F may contribute to the differences between them. The pharmacodynamics experiments results showed the TLE of AR and VR affected the different liver cholate-associated transporters mRNA expression (TLE of AR up-regulated CYP7A1, TLE of VR down-regulated FXR and BSEP), the TLE of AR and VR had an effect to regulate biochemical indicators (AST, ALT, ALP, TBA) of liver function, and TLE of VR was better than TLE of AR in reducing the expression of inflammatory factors (IL-6 and IL-1β). ConclusionThe liver protection of AR and VR have been confirmed, but the differences of material basis and mechanism of drug efficacy needed further study to guarantee formulation research and provide theoretical references for clinical rational applications of AR and VR.

UPLC/Q-TOF MS Screening and Identification of Antibacterial Compounds in Forsythia suspensa (Thunb.) Vahl Leaves.

Forsythia suspensa (Thunb.) Vahl (F. suspensa) is a traditional Chinese medical herb and only its fruit is currently used in clinical therapies. However, the discarded parts like leaves also contain a large number of active components. In this study, we used macroporous adsorption resin to enrich the effective components from F. suspensa leaves. The separated active compounds were then identified and quantified by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC/Q-TOF MS) and high-performance liquid chromatography Active components with antibacterial properties extracted from F. suspensa leaves were confirmed in vitro and the corresponding mechanisms were explored. In sum, a stable and effective method for extracting antibacterial active components from F. suspensa leaves was established in this study, which proved the practicability of F. suspensa leaves as traditional Chinese medicine and is conducive to the more comprehensive utilization of the plant.

Purification of Crocin-I from Gardenia Yellow by Macroporous Resin Columns In-Series and Its Antidepressant-Like Effect

In this study, the purification of crocin-I from Gardenia yellow by macroporous resin columns in-series was systematically investigated. The in-series macroporous adsorption resins consisting of XAD 4 and XAD 1600N resins were selected on the basis of the evaluation of performance and separation characteristics of 17 kinds of resins, including the adsorption capacities, desorption ratio, and separation degree. According to the analysis results, the optimum conditions were as follows: bed volume ratio of XAD 4 and XAD 1600N resins, sample volume, flow rate, and methanol concentration were 3 : 1, 5 BV, 15 BV/h, and 70%, respectively (BV was the bed volume of XAD 4 resin). After one run treatment, the separation degree of crocin-I at 254 nm and 440 nm decreased from 1.92 to 0.08 ( D C − 254 nm ) and from 0.71 to 0.36 ( D C − 440 nm ), respectively. The results showed that the in-series macroporous resins revealed a high capacity in the purification of crocin-I. Meanwhile, in the animal experiment, the forced swimming tests were regulated by crocin-I and Gardenia yellow, which demonstrated that crocin-I was the main constituent of Gardenia yellow and had potential antidepressant biological activities.

Study on the mechanism of anaerobic fluidized bed microbial fuel cell for coal chemical wastewater treatment.

The coal chemical wastewater (CCW) was treated by anaerobic fluidized bed microbial fuel cell (AFB-MFC) with macroporous adsorptive resin (MAR) as fluidized particle. Isosteric heat calculation and molecular dynamics simulation (MDS) have been performed to study the interaction between organics of CCW and MAR. The isosteric heat of MAR to m-cresol was the largest at 65.4961kJ/mol, followed by phenol. Similarly, the diffusion coefficient of m-cresol on MAR was the largest, which was 0.04350 Å2/ps, and the results were verified by the kinetic adsorption experiments. Microbial community analysis showed that the dominant bacteria in activated sludge of MFC fed with CCW were acinetobacter, aeromonas, pseudomonas and sulfurospirillum. The synergistic cooperation of bacteria contributed to improving CCW degradation and the power generation of MFC. Headspace-gas chromatography-mass spectrometry (HS-GC-MS) was used to detect intermediate of organics in CCW. It was proved that the intermediate of m-cresol degradation was 4-methyl-2-pentanone and acetic acid, and the intermediate of phenol degradation included cyclohexanone, hydroxyhexanedither and hydroxyacetic acid. Combined with the highest occupied molecular orbital (HOMO) analysis results of organic matter obtained by molecular simulation, the degradation pathway of organic matter in CCW was predicted. The energy of organics degradation pathway was analyzed by Materials Studio (MS) software, and the control step of organics degradation was determined.

Purification and the effects on structure and bioactivity for polysaccharide from Actinidia valvata Dunn. using macroporous adsorption resin

Resin AB-8 was screened from seven resins with different polarity and particle size because of its better decoloration and deproteinization efficiency to polysaccharide from the root of Actinidia valvata (AvPs), and then the optimized parameter conditions were applied by the single-factor experiments and were shown as follows: a concentration of crude polysaccharide of 3 mg/mL, an adsorption time of 40 min, an initial pH of 7.0, a flow rate of 1.5 BV/h. In addition, the results of HPGPC, FT-IR, PMP-HPLC-DAD, 1H NMR and Congo red test showed that the sugar chain, structural group, monosaccharide composition and triple-helix conformation structure of AvPs were not broken during resin AB-8 treatment. Moreover, AvPs could keep the stability of biological activity after the resin AB-8 treatment, and revealed an obviously antioxidant and anticancer activities in vitro as well. This research suggests that this method is promising for the deproteinization and decolorization of AvPs which could be explored as a potential antioxidant or anticancer agent for use in functional food or medicine.

Purification of polysaccharide from Solanum nigrum L. by S-8 macroporous resin adsorption

To study the purification conditions of polysaccharide from Solanum nigrum L. by S-8 macroporous adsorption resin. The effect of multi-factors on the purification of polysaccharide from Solanum nigrum were studied by static - dynamic adsorption and desorption methods with indexes of adsorption and desorption rates. The optimized purification process condition was as follows: the concentration of sample was 5 mg/mL, pH level was 7.0, temperature was 30 °C, the adsorption velocity was 0.5 mL/min, the maximun loading amount was 10 BV, 0.5 mol/L NaCl solution 5BV was used as eluent, elution flow rate was 4 mL/min. With this condition, the purity of Solanum nigrum polysaccharide was 91.00%, which was 1.989 times higher than before. S-8 macroporous resin can be used to separate and purify the solanum nigrum polysaccharide and improve the purity and quality of polysaccharides.

Tetrabutylammonium bromide-based hydrophobic deep eutectic solvent for the extraction and separation of dihydromyricetin from vine tea and its inhibitory efficiency against xanthine oxidase.

In this study, deep eutectic solvent oscillation-assisted extraction (DES-OS) combined with macroporous resin adsorption and desorption technology was used to achieve the rapid green extraction and separation of the characteristic component dihydromyricetin (DMY) from vine tea. Multivariate data analysis showed that the DES system composed of tetrabutylammonium bromide (N444Br) and pyruvic acid (molar ratio 1 : 2) had good extraction performance for DMY. The influence parameters of DES-OS were studied, and optimized by the single-factor test and response surface methodology (RSM) with Box-Behnken design (BBD). The extraction model of DMY was established and verified. The results showed that the extraction yield of DMY could reach 40.1 mg g-1 under the optimal conditions (DES water contents of 71.18%, extraction time of 2.80 h, extraction temperature of 46.40 °C), which is in good agreement with the predicted value. In addition, Fourier transform infrared spectroscopy (FT-IR) was used to characterize the solvent before and after extraction. Scanning electron microscopy (SEM) results further confirmed that tetrabutylammonium bromide:pyruvate enhanced the destruction of the cell wall structure, resulting in the release of more DMY. Furthermore, different macroporous resins were selected for the separation of DMY for the DES-OS extract, and it was found that the DM301 resin had the ideal recovery performance under optimized dynamic condition. Finally, the product was found to have an inhibitory effect against xanthine oxidase (XO) as a mixed-type competitive inhibitor with IC50 values of (5.79 ± 0.22) × 10-5 mol L-1. The inhibitory mechanisms of DMY on XO were explored by enzyme kinetics, spectroscopy, molecular docking and molecular dynamics analysis approaches, which provided a theoretical basis for the above inhibition assays.

Carbon dot-functionalized macroporous adsorption resin for bifunctional ultra-sensitive detection and fast removal of iron(III) ions.

Heavy metal pollution has spread around the world with the development of industry, posing a major threat to human health. It is urgent to design and fabricate bifunctional materials for detection and adsorption of heavy metal ions. Herein, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres, a kind of common macroporous adsorption resin (MAR), were employed as the matrix, and carbon dots (CDs) with excellent optical properties were grafted onto the surface of MAR by surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated "thiol-yne" click chemistry. The synthesized MAR@poly(PA)@CD could produce fluorescence quenching with Fe3+. A simple fluorescence spectrometric method for detection of Fe3+ was established. The fluorescence intensity of MAR@poly(PA)@CD decreased linearly with the concentration of Fe3+ in the range of 0-70 nmol L-1, with a limit of detection (LOD) of 6.6 nmol L-1, which had the potential for trace detection. In addition, after SI-ATRP modification, many adsorption sites were generated on the surface of MAR, and the adsorption capacity for Fe3+ was 23.8 mg g-1. Isothermal and kinetic adsorption experiments were more consistent with the Langmuir model (r = 0.9992) and pseudo-second-order model (r = 0.9902), indicating that the adsorption was monolayer adsorption and chemical adsorption, respectively. MAR@poly(PA)@CD with dual functions of detecting and adsorbing Fe3+ was successfully prepared, showing great application prospects in the environmental field.

Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography

Anthocyanins are often targets in polyphenol analysis. However, it's hard to effectively separate anthocyanin from copigments such as phenolic acid and flavanols due to their similar structure. Thus, a cation exchange chromatography with 001 × 7 has been developed, which is available for anthocyanins isolation both on a small and large scale. The optimal process condition of anthocyanins isolation was determined. Compared to the macroporous adsorbent resins and Strong Cation Exchange resin (SCX), 001X7 shows greater economic advantages in large-scale purification of anthocyanins. More than 95% purity of the anthocyanin fraction can be achieved through this approach. This method shows a path to provide large quantities of copigments-free anthocyanins from mulberry polyphenols for the further study of its biological effects and may be extended to other analytical methods of polyphenol isolation from other plant materials.

Extration, identification and stability ananlysis of anthocyanins from organic Guizhou blueberries in China

Guizhou Majiang organic blueberries from China are a prevalent fruit in the health industry and have high anthocyanin contents. This study used the ethanol extraction method, ultrasonic-assisted extraction, AB 8 macroporous resin adsorption purification, and the addition of 3% citric acid to extract anthocyanins, and through single-factor tests and orthogonal tests, the optimum extraction conditions of anthocyanins from blueberries was determined. The optimal extraction conditions were as follows: the extraction temperature was 40 °C, the ethanol volume fraction was 80%, the solid-liquid ratio was 1:10, the ultrasonic extraction time was 60 min, and the extraction amount was 2.56 mg/g. Anthocyanins were identified by HPLC, and the effects of storage conditions on their contents were investigated. K+, Cu2+, and Fe2+ had little influence on anthocyanin contents, while hydrogen peroxide had a highly destructive effect. With increasing illumination time, the retention rate of anthocyanins decreased gradually; the higher the temperature was, the stronger the damaging effect on anthocyanin contents, and the appropriate storage temperature was determined to be -4 °C or below. The absorbance was the highest when pH =2. Taken together, the results of our study revealed the optimal extraction process and appropriate preservation conditions of anthocyanins from organic Guizhou blueberries.

Optimization of Green Extraction Process of Total Flavonoids from Dendrobium Officinale Flower

Objective: To make full use of the flavonoids in Dendrobium officinale flowers, a green and efficient extraction process was developed. Methods: Taking the yield of flavonoids from Dendrobium officinale flowers as an index, using new deep eutectic solvents, based on single factor experiments and response surface methodology, the extraction process of total flavonoids was optimized. And macroporous adsorption resins were used to investigate the recycling capacity of DESs. Results: DES composed of choline chloride and ethylene glycol (molar ratio 1:4) had the highest yield. The yield reached 22.457 mg/g under the optimal extraction conditions (water content 18.9 %, temperature 88.2 ℃, liquid-solid ratio 23.3:1 mL/g, time 38.0 min). The adsorption rate and desorption rate of ADS-8 for total flavonoids were 78.32 % and 83.55 %, respectively, and the recycling rate of recovered DES reached 95.47 %. Conclusion: The deep eutectic solvent is an efficient and green method that could be recycled to extract the total flavonoids from Dendrobium candidum flowers.

A novel approach for synchronous transformation and extraction of psoralen from fig (Ficus carica L.) leaves based on polarity of different macroporous adsorption resins

In this study, using fig leaves as materials, strong acidic cation exchange resin of D072 with –SO3H functional group was selected as catalyst to promote the conversion of psoralen-glucoside (PO) to psoralen (PSO), and the strong acidic cation exchange resin transformation method (SACERTM) was developed for efficient extraction of PSO from fig leaves. Under the optimized extraction conditions, the yield of PSO was 36.74 mg/g. The transformation efficiency of PO to PSO reached 70.41% using 2 g of D072 resin as catalyst. Then, PSO in fig extract was enriched efficiently by X-5 macroporous adsorption resin (MAR). After eluting with 14-fold of column volume of 70% ethanol at 0.64 BV/h of elution flow rate, the purity of PSO was 24.8-fold higher than before enrichment. The results show that SACERTM combined with MAR purification is an efficient method for extracting and enriching PSO from fig leaves.

Adsorption Equilibrium and Thermodynamics of Tea Theasinensins on HP20-A High-Efficiency Macroporous Adsorption Resin.

The separation and preparation of theasinensins have been hot spots in the field of tea chemistry in recent years. However, information about the mechanism of efficient adsorption of tea theasinensins by resin has been limited. In this study, the adsorption equilibrium and thermodynamics of tea theasinensins by a high-efficiency macroporous adsorption HP20 resin were evaluated. The adsorption of theasinensin A, theasinensin B, and theasinensin C on HP20 resin were spontaneous physical reaction processes. Adsorption processes were exothermic processes, and lowering the temperature was beneficial to the adsorption. The Freundlich model was more suitable to describe the adsorption of tea theasinensins. The adsorption equilibrium constant and maximum adsorption capacity of theasinensin A were significantly higher than theasinensin B and theasinensin C, which indicated that the adsorption affinity of theasinensin A was stronger than that of theasinensin B and theasinensin C. The phenolic hydroxyl groups and intramolecular hydrogen bonds of theasinensin A were more than those of theasinensin B and theasinensin C, which might be the key to the resin’s higher adsorption capacity for theasinensin A. The HP20 resin was very suitable for efficient adsorption of theasinensin A.

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice.

Red yeast rice is consumed as a medicinal food to lower blood lipids. Besides, it is used to color food, make wine, etc. In this study, water‐soluble oligosaccharides in red yeast rice were extracted by ultrasonic‐assisted extraction method. The parameters to extract oligosaccharides from red yeast rice were optimized by the Box‐Behnken design under the following optimal extraction conditions: extraction temperature, 60°C; extraction time, 97 min; and liquid/material ratio, 25 ml/g. The structure and the antioxidant activity of the new oligosaccharide were preliminarily investigated. Total carbohydrates extracted from red yeast rice with 80% ethanol–water solution (v/v) were first removed from pigments using D101 macroporous adsorption resin. The total sugar contents were then purified by DE52 resins and Sephadex G‐25 resins to obtain red yeast rice oligosaccharides, coded as RYRO1. Structural characterization experiments indicated that RYRO1 is an oligosaccharide with a weight average molecular weight of 874 Da and a theoretical degree of polymerization of 4.86. RYRO1 is composed of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.248:0.019:1:0.026. The ABTS, DPPH, and hydroxyl free radical scavenging assays showed antioxidant nature of RYRO1.

Large-scale targetedly isolation of biflavonoids with high purity from industrial waste Ginkgo biloba exocarp using two-dimensional chromatography coupled with macroporous adsorption resin enrichment

Seeds and leaves of Ginkgo biloba have been widely applied for industrial application, viewing and medicinal resource. However, the exocarp of G. biloba is usually abandoned and considered as industrial waste. In this work, an efficient and industrially viable protocol for large-scale targetedly isolation of bioactive biflavonoids with high purity from industrial waste G. biloba exocarp was established firstly. Macroporous adsorption resin was used for enriching bioflavonoids, and targeted on-line recognition of biflavonoids was based on their characteristic UV absorption at 210, 270 and 330 nm. Two-dimensional preparative normal phase/reversed phase HPLC-DAC50 system was applied for isolating biflavonoids. A total of 3 biflavonoids including bilobetin, ginkgetin and isoginkgetin were isolated and their purity > 99.0%, dozens or hundreds of milligrams were obtained in one isolation within 30 min. This work will not only provide an effective method for isolating large-scale high purity biflavonoids, but contribute to making full use resources of industrial waste G. biloba exocarp.

Integration of Choline Chloride-Based Natural Deep Eutectic Solvents and Macroporous Resin for Green Production of Enriched Oil Palm Flavonoids as Natural Wound Healing Agents.

Huge quantities of oil palm (Elaeis guineensis Jacq.) leaves (OPL) are generated as agricultural biomass from oil palm plantations. OPL are known to contain significant amounts of flavonoids. For maximal exploitation of these valuable antioxidant compounds, an innovative and sustainable extraction method employing natural deep eutectic solvents (NaDES) combined with ultrasonic assisted extraction was developed. Various NaDES composed of choline chloride as the hydrogen bond donor (HBD) and 1,2 propanediol (PD), 1,4 butanediol (BD), glycerol (GLY), glucose (GLU), maltose (MAL), and lactic acid (LA) as the hydrogen bond acceptor (HBA) were synthesized. The influence of these compositions, the methods of their synthesis, molar ratios, and water contents on their capacity to extract flavonoids from OPL was evaluated. Based on the results, it was found that methods which incorporate a heating step produced NaDES with the best capacity to extract OPL flavonoids. These thermal methods combined with molar ratios of 1:3 or 1:4 and water contents of 17 to 50% were found to be the optimal conditions for preparing NaDES, specifically when applied to the PD, BD, and GLY NaDES. Subsequently, UHPLC-UV/PDA-MS/MS analysis revealed NaDES extracts recovered by macroporous adsorption resin XAD7HP were able to optimally extract at least twelve luteolin and apigenin derivatives in OPL NaDES extracts prepared from glycerol and 1,4-butanediol demonstrated better and comparable efficiency as aqueous methanol in extracting flavonoids from OPL. The in vitro studies of antioxidant and wound healing properties supported these findings by exhibiting good free radical scavenging, cell proliferation, and migration activities. Additionally, the NaDES extracts also showed non-cytotoxicity effects at 1000 µg/mL and below on 3T3 fibroblast cells. Results of the study showed that NaDES could be a promising eco-friendly green solvent to extract bioactive OPL flavonoids that have great potential for applications as wound healing agents.