Macrofauna affects carbon and nutrient cycling, modifies soil structure and water-holding capacity, and contributes to the provision of various ecosystem services [6]. Agroforestry systems with diverse tree cover support macrofauna diversity that supports sustainable soil ecosystems [3]. Research conducted by [5] that agricultural practices associated with agroforestry systems in Honduras resulted in relatively abundant and diverse soil macrofauna communities. This study examines the diversity of macrofauna in the agroforestry system because in the agroforestry system various types of stands will produce plant litter. This research was conducted in Sumberejo Village, Baturetno District, Wonogiri Regency. This research was conducted on teak agroforestry, mahogany agroforestry, mixed agroforestry, and seasonal crops that did not apply the agroforestry system. Diversity of macrofauna using pitfall trap and monolith method. This study examines soil organic carbon because it is used as energy by soil macrofauna. This study aims to determine the relationship between soil macrofauna and soil organic carbon. The relationship between soil macrofauna and soil organic carbon is negatively correlated, mahogany agroforestry land organic carbon is included in the high category but the total macrofauna individuals found are few, but the macrofauna diversity index of mahogany agroforestry land is high.