Macrofungal Diversity Research Articles

Forest Type and Climate Outweigh Soil Bank in Shaping Dynamic Changes in Macrofungal Diversity in the Ancient Tree Park of Northeast China.

The community structure of macrofungi is influenced by multiple complex factors, including climate, soil, vegetation, and human activities, making it challenging to discern their individual contributions. To investigate the dynamic changes in macrofungal diversity in an Ancient Tree Park located in Northeast China and explore the factors influencing this change, we collected 1007 macrofungi specimens from different habitats within the park and identified 210 distinct fungal species using morphological characteristics and ITS sequencing. The species were classified into 2 phyla, 6 classes, 18 orders, 55 families, and 94 genera. We found macrofungal compositions among different forest types, with the mixed forest displaying the highest richness and diversity. Climatic factors, particularly rainfall and temperature, positively influenced macrofungal species richness and abundance. Additionally, by analyzing the soil fungal community structure and comparing aboveground macrofungi with soil fungi in this small-scale survey, we found that the soil fungal bank is not the main factor leading to changes in the macrofungal community structure, as compared to the influence of climate factors and forest types. Our findings provide valuable insights into the dynamic nature of macrofungal diversity in the Ancient Tree Park, highlighting the influence of climate and forest type.

A Statistical Approach to Macrofungal Diversity in a Mediterranean Ecosystem of the Iberian Peninsula Dominated by the Holm Oak (Quercus ilex L. subsp. ballota (Desf.) Samp.)

The fungal biodiversity associated with a stable plant community appears to vary from year to year. To analyse the annual behaviour in the formation of fruiting bodies, a statistical study of the data obtained for 4 years (2009–2012) in an apparently undisturbed Mediterranean ecosystem dominated by the holm oak (Quercus ilex L. subsp. ballota (Desf.) Samp.), located in the Midwest of the Iberian Peninsula, was carried out. These data were related to the main meteorological variables. The 150 species collected showed a significant annual, monthly, and weekly difference in their fruiting during the collection period. All this implies a variation in the annual fungal fruiting which can modify the moment of when maximum peaks of fruiting appear, their duration, and the number of species that compose them. In addition, the results make it possible to establish an annual behaviour pattern, with sporocarp formation throughout the year and four fruiting groups (two of them in the dry season). They also allow for inferring a possible response to climate change, with a delay in the fruiting of the autumn-winter group and earlier fruit bearing in the winter-spring group.

Status of macrofungal diversity in the wet evergreen forests of Agasthyamala Biosphere Reserve, Western Ghats, India

Agasthyamala Biosphere Reserve is a part of Western Ghats (India), has diverse ecosystems and constitutes an important biogeographical ‘hotspot’ which is well known for its species richness and endemism. Since limited information was available on the mycoflora in this area, a survey was conducted to evaluate the macrofungal diversity in the wet evergreen forests of the Agasthyamala Biosphere Reserve. The survey was carried out during the monsoon and post-monsoon seasons of 2021–2022 and revealed the existence of 62 macrofungal species belonging to 43 genera, 24 families, and eight orders. Out of the eight orders, seven orders belong to the division Basidiomycota and the other order Xylariales belongs to Ascomycota. The family Polyporaceae was identified as the dominant family. The survey also noted the presence of saprotrophic and mycorrhizal fungi. Among the identified species, the maximum density was of Panellus pusillus (6.08) followed by Microporus xanthopus (5.38). Microporus xanthopus (82.14%) exhibited the maximum frequency of occurrence and was identified as the most common species. Coprinellus disseminatus was the most abundant species among macrofungi. The assessment of macrofungal diversity using the Shannon biodiversity index resulted in a value of 2.99, indicating a rich and diverse fungal population within the forest. This finding emphasizes the significant role of the forest ecosystem in supporting a wide variety of fungi

Macrofungal diversity in small-holder oil palm plantations on tropical peatlands

Abstract. Ekyastuti W, Astiani D, Ekamawanti HA, Jamiat. 2023. Macrofungal diversity in small-holder oil palm plantations on tropical peatlands. Biodiversitas 24: 3454-3461. Mushrooms play an important role in ecosystems and human life. They can be found in various places including in oil palm plantations. The aim of this study was to survey and identify the population of macroscopic mushrooms or macrofungi in small-holder oil palm plantations on tropical peatlands in Kampung Baru Village, Kubu Raya District, West Kalimantan, Indonesia using the field survey method. In the research area, a dam has been constructed to regulate the water level at 30 cm, 40 cm, 50 cm, and 60 cm from the surface. Sampling was carried out in these four conditions. The study revealed the presence of 34 species of macrofungi belonging to the Basidiomycota. A groundwater level ranging from 40-60 cm from the surface was identified as favorable for macrofungal growth. Meanwhile, a groundwater level of 30 cm from the surface was found to be unsuitable for macrofungal growth in the peatlands. The level of macrofungal species diversity in small-holder oil palm plantations on the peatlands was determined to be moderate.

The macrofungal diversity and its potential from the karst forest of Kalipoh Village, Kebumen District, Indonesia

Abstract. Armadhan WS, Sari SP, Aji MYMB, Permatasari DP, Amalia BW, Berlin GE, Aszar AS, Indrawan M, Pradhan P, Setyawan AD. 2023. The macrofungal diversity and its potential from the karst forest of Kalipoh Village, Kebumen District, Indonesia. Intl J Trop Drylands 7: 98-105. Indonesia is one of the world's most biodiverse countries, with fungi being one of its most diverse groups of organisms. Many fungi species have been identified and have potential benefits for both living things and the environment. Kalipoh Village Forest, located in the karst area of Ayah Sub-district, Kebumen District, Central Java, Indonesia, offers suitable environmental conditions that provide habitat for various species of fungi. The objective of this study was to determine the diversity and potential use of macrofungi in Kalipoh Village Forest. Data collection was carried out using the survey method, where every encountered fungus was observed and its cap, gills, stalk, color, odor, substrate, and growth habits were recorded. Macrofungal diversity was calculated using the Shannon-Wiener Diversity Index, Simpson’s Diversity Index, Richness Index, and Evenness Index Formula. The exploration results obtained 34 species of macrofungi from 22 genera and 18 families, with most of the species found on weathered logs and leaf litter. Two genera, namely Marasmius and Marasmiellus, were quite common. Based on the index value calculation, the diversity of macrofungi in this area was in the medium category (H'=2.695). It had a high index of richness (R=5.252) and evenness (E=0.764). The high evenness of the species indicates lower dominance, which can also be observed from the results of the high Simpson’s Diversity Index value (0.910). A total of 24 species of macrofungi were known to have the potential as food and medicine.

Diversity and biological characteristics of macrofungi of district Bajaur, a remote area of Pakistan in the Hindu Kush range

The present study was carried out to document the diversity and ecological characteristics of macrofungi of Bajaur, Pakistan. The diversity of macrofungi comprised 51 species belonging to 22 families and 37 genera. The families Agaricaceae (7 species) and Psathyrellaceae (7 species) were found dominant followed by Tricholomataceae (4 species), Fomitopsidaceae and Polyporaceae (4 species each) and Amanitaceae (3 species). White (23 species), brown (11 species), and yellow were the most prevalent morphological colours in basidiocarps (8 species). Among the identified species, 32 were saprophytic in nutrition followed by 7 parasitic, 6 saprophytic and parasitic both, while 6 mycorrhizal that make association with higher plants. The distribution of macrofungal species in the three tehsils of Bajaur was also evaluated based on Shannon diversity index, Simpson diversity index and evenness. The highest Shannon diversity index and Simpson diversity index were found for tehsil Utman Kheil at 3.73 and 0.97, while the maximum value of evenness for tehsil Khar with 0.92 value. The results indicate a very high species richness of the study site. Four species out of the total were identified to be new reports from Pakistan. This survey's findings suggested that there is a wide variety of macrofungi that might be used as food and alternative medications if further research is carried out.

Macrofungi in Some Areas of Borail Reserve Forest of Dima Hasao District, Assam

Macrofungi are ecologically resourceful group of organisms exists in every possible ecosystem. Borail Reserve Forest of Dima Hasao district, Assam is endowed with typical Sub tropical monsoon climate which provide adequate habitat for the growth and development of various types of vegetation as well as diverse species of macrofungi. The study attempts to assess the macrofungal diversity in certain areas of Borail Reserve Forest as it is yet to explore. The investigation was conducted in the year 2021 (June-December). Sampling was done using quadrat method each measuring 100×100m2. During the survey, a total of 21 species belonging to 15 families were encountered and identified up to species level i.e. Auriculariaceae, Clavulinaceae, Psathyrellaceae, Fomitopsidaceae, Ganodermataceae, Agaricaceae, Lyophyllaceae, Marasmiaceae, Polyporaceae, Phallaceae, Pluteaceae, Schizophyllaceae, Stereaceae, Tremellaceae and Xylariaceae. It is found that 20 species belong to basidiomycota group and only 1 species belongs to ascomycota group. However, Marasmiaceae and Polyporaceae family are found to be the most dominant family of macrofungi in the region. The inference of the study revealed the diverse treasure of macrofungi of Borail Reserve Forest. The record of macrofungi of the study gives the baseline information for the biological diversity. The significance of macrofungi is not only to balance the ecosystem dynamics but also in human diet and beneficial impact to health, which increases the need for the conservation of this valuable resource.

Inocybe istriaca sp. nov. from Brijuni National Park (Croatia) and Its Position within Inocybaceae Revealed by Multigene Phylogenetic Analysis

Integrative taxonomic studies of macrofungal diversity in the Brijuni National Park (Istria County, Croatia) led to the discovery of a second species of Inocybe (Agaricales, Inocybaceae) new to science. Inocybe istriaca sp. nov. is described on the basis of morphological, ecological, and multigene phylogenetic analyses, and its placement within the family Inocybaceae is discussed. The combination of most important morphological characters that distinguish I. istriaca from the other similar Inocybe species are smooth, (sub)amygdaliform, (sub)phaseoliform, or ellipsoid basidiospores (ca. 8.5–12 × 5–7 μm), large basidia (36–45 × 9–15 μm), mostly (sub)fusiform and weakly thick-walled (up to 1.5 μm) metuloid pleurocystidia, and lamellar edge and stipe apex partially covered by a dark resinous substance. The species was collected on the edge of grassland and Mediterranean evergreen holm oak (Quercus ilex) forest. In this study, a total of 14 DNA sequences from four Inocybe species were generated. Two-gene (ITS, LSU) and four-gene (ITS, LSU, rpb2, tef1) phylogenetic analyses confirmed the status of I. istriaca as an independent species.

Macrofungi in Stands of the Endemic Pine Pinus peuce as Inferred from Morphological and Molecular Data

Pinus peuce (Balkan, Macedonian or Molika pine) is an endemic tree for the Balkans and a Tertiary relict, one of the two European five-needled pines of the subgenus Strobus (white pines). This work summarizes the data on macrofungal diversity in natural forests and artificial plantations of Macedonian pine in Bulgaria, based on three year survey of epigeous and hypogeous fungi in selected sampling plots, and combining morphology-based and molecularly inferred identification. The study registered 190 species in total. Higher diversity of macrofungi was recorded on sampling plots in natural stands compared to artificial plantings. Nine species are reported for the first time for the Bulgarian mycobiota. The paper provides the first public set of sequences of macrofungi of P. peuce stands, comprising 62 accessions.

Studies on macrofungi diversity and discovery of new species of Abortiporus from Baotianman World Biosphere Reserve.

This research focuses on macrofungi in Baotianman Biosphere Reserve and their relationships with plant ecosystems. The findings demonstrate the reserve's macrofungal resources. The study collected 832 specimens, among which 351 macrofungi species were identified, belonging to six classes, 19 orders, 54 familiae, and 124 genera, and one new species of Abortiporus was found. Among them, 11 familiae with a total of 231 species were dominated, accounting for 20.37% of the total number of familiae and 65.81% of the total number of species; 14 genera with a total of 147 species were dominated, accounting for about 11.38% the total number of genera and 41.88% of the total number of species. The richness of macrofungi at the species level was considerably different across the four vegetation types in the reserve, showing that the vegetation types had a bigger influence on macrofungi. In the evaluation of macrofungal resources, a total of 196 species of edible fungi, 121 species of medicinal fungi, 52 species of poisonous fungi, and 37 species of macrofungi with unclear economic value were counted. Abortiporus baotianmanensis is a new species of podoscyphaceae in the genus Abortiporus. The new species display the reserve's richness. Next, the project seeks to generate and conserve macrofungal resources.

The ghost fungus Omphalotus nidiformis (Berk.), new to Indonesia, poisoned foragers

Most Indonesian wild mushrooms remain unexplored, including genus Omphalotus. Omphalotus nidiformis (Omphalataceae), which is primarily distributed in arid parts of Australia and is globally known as a poisonous mushroom. No prior report exists on this species' occurrence in Indonesia. In November 2021, Indonesian foragers consumed the pleurotoid mushroom, which was misidentified as Pleurotus, and were poisoned at Mount Tangkuban Perahu, West Java. This study aimed to ensure the taxonomical position of the poisonous macrofungus and record the poisoning event to increase public awareness in Indonesia. Fresh basidiomata materials were assessed for morphological and molecular information. The specimen's morphology was described based on its macroscopic and microscopic characteristics. The molecular and phylogenetic approach used internal transcribed spacer (ITS) 4/5 sequence data. Combined morphological and molecular analyses confirmed the taxonomical position of our specimen as O. nidiformis (Berk.). Morphology illustrations and the phylogenetic tree of O. nidiformis are provided. This is the first report on O. nidiformis distribution in Indonesia, and this study contributed to Indonesia's macrofungi diversity data. Schima wallichii was confirmed as a host for O. nidiformis. Additionally, the poisoned foragers had symptoms of nausea and vomiting, as well as weakness, perspiration, and trembling. Our report indicated the urgent need for mycological dissemination to the public in Indonesia regarding O. nidiformis identification.

Macrofungal Diversity and Distribution Patterns in the Primary Forests of the Shaluli Mountains.

The Shaluli Mountains are located in the southeastern part of the Tibetan Plateau at an elevation of 2500-5000 m. They are characterized by a typical vertical distribution of climate and vegetation and are considered a global biodiversity hotspot. We selected ten vegetation types at different elevation gradients representing distinct forests in the Shaluli Mountains to assess the macrofungal diversity, including subalpine shrub, Pinus spp., Populus spp., Pinus spp. and Quercus spp., Quercus spp., Abies spp., Picea spp. and Abies spp., Picea spp., Juniperus spp., and alpine meadow. In total, 1654 macrofungal specimens were collected. All specimens were distinguished by morphology and DNA barcoding, resulting in the identification of 766 species belonging to 177 genera in two phyla, eight classes, 22 orders, and 72 families. Macrofungal species composition varied widely among vegetation types, but ectomycorrhizal fungi were predominant. In this study, the analysis of observed species richness, the Chao1 diversity index, the invsimpson diversity index, and the Shannon diversity index revealed that the vegetation types with higher macrofungal alpha diversity in the Shaluli Mountains were composed of Abies, Picea, and Quercus. The vegetation types with lower macrofungal alpha diversity were subalpine shrub, Pinus spp., Juniperus spp., and alpine meadow. The results of curve-fitting regression analysis showed that macrofungal diversity in the Shaluli Mountains was closely related to elevation, with a trend of increasing and then decreasing with rising elevation. This distribution of diversity is consistent with the hump-shaped pattern. Constrained principal coordinate analysis based on Bray-Curtis distances indicated that macrofungal community composition was similar among vegetation types at similar elevations, while vegetation types with large differences in elevation differed significantly in macrofungal community composition. This suggests that large changes in elevation increase macrofungal community turnover. This study is the first investigation of the distribution pattern of macrofungal diversity under different vegetation types in high-altitude areas, providing a scientific basis for the conservation of macrofungal resources.

INVENTORY OF MACROFUNGI DIVERSITY ON THE CANOPY TRACK AND CIPADARANTEN 1 TRACK IN THE PPKA BODOGOL AREA, SUKABUMI, WEST JAVA

The inventory of macrofungi diversity on two routes, namely the Kanopi route and the Cipadaranten 1 route at the Bodogol Nature Conservation and Education Center (PPKA), aims to determine the diversity of macrofungi species that live in the area. The research method used is direct observation in the Canopy and Cipadaranten 1 route areas. With the condition of the trees on both routes which are still quite dense and the soil area is still damp due to the cover of the trees, there is great potential for the growth of various types of macrofungi. The results obtained during research on both routes and identification produced 24 species of macrofungi Microporus sp. 1, Microporus sp. 2, Microporus sp. 3, Trametes sp.1, Lenzites sp, Ganoderma sp., Ganoderma boninense, Trametes sp.2, Microporus xanthopus, Hexagonia sp, Trametes parvispora, Microporus sp. 4, Hygrocybe aurantiosplendens, Hygrocybe sp., Marasmius sp. 1, Collybia aurea, Crepidotus sp., Volvariella sp., Marasmius sp. 2, Lycoperdon sp., Marasmiellus sp. , Coprinus sp. , Stereum sp., Auricularia sp. The results obtained prove that the diversity of macrofungi types on the Kanopi and Cipadaranten 1 routes in PPKA Bodogol, West Java is very diverse and varied.

How future climate and tree distribution changes shape the biodiversity of macrofungi across Europe

AbstractAimClimate change is affecting biodiversity at an accelerating rate. Despite the importance of fungi in ecosystems in general, and in the global carbon and nitrogen cycle in particular, there is little research on the response of fungi to climate change compared with plants and animals. Earlier studies show that climatic factors and tree species are key determinants of macrofungal diversity and distribution at large spatial scales. However, our knowledge of how climate change will affect macrofungal diversity and distribution in the future remains poorly understood.LocationEurope.MethodsUsing openly available occurrence data of 1845 macrofungal species from eight European countries (i.e. Norway, Sweden, Finland, Denmark, Netherlands, Germany, France and Spain), we built ensemble species distribution models to predict macrofungal response to climate change alone and combined climate and tree distribution change under the IPCC special report on 2080 emissions scenarios (SRES A2 and B2).ResultsConsidering climate change alone, we predict that about 77% (74.1%–80.7%) of the modelled species will expand their distribution range, and around 57% (56.1%–58.4%) of the modelled area will have an increase in macrofungal species richness. However, when considering the combined climate and tree species distribution change, only 50% (50%–50.9%) of the species are predicted to expand their distribution range and 49% (47.4%–51.1%) of the modelled area will experience an increase in macrofungal species richness.Main ConclusionsOverall, our models projected that large areas would exhibit increased macrofungal species richness under future climate change. However, tree species distribution might play a restrictive role in the future distributional shifts of macrofungi. In addition, macrofungal responses appear heterogeneous, varying among species and regions. Our findings highlight the importance of including tree species in the projection of climate change impacts on the macrofungal diversity and distribution on a continental scale.

Diversity Of Macrofungi from Melghat Forest Amravati Maharashtra

In the present investigations authors collected many fungal samples from Melghat forest of Amravati at Satpuda ranges of Maharashtra and Madhya Pradesh. In the forest area close to Amravati, Maharashtra, the current study was conducted to map out the diversity and distribution of native macrofungi. During the wet and early dry seasons of July to October 2022, samples were collected and these samples were collected from low and high altitudinal ranges. Various sites in the research area of forest investigated and used to collect various wild fungus species. According to reliable sources and microscopic characteristics, the macro fungi species Ganoderma sp., Hexagonia sp., Microporus sp., and Phellinas sp. were identified. The early dry season saw the greatest abundance of Ganoderma sp. among these species. The study showed that the macro fungal diversity has observed. Maharashtra region required for conservation efforts, particularly for species that are edible and therapeutic.

Macro-fungi diversity in Thulo Ban Community Forest of Arjam, Myagdi District, Nepal

Macro-fungi produce large fructifications that are visible without the help of a microscope. They play an important role in the conservation of forest ecosystems and biodiversity. The current study deals with the diversity of macro-fungi in the subtropical mixed forest of Arjam, Myagdi District. The study was conducted from June to September 2020, at a height of 1250 to 1450 meters above sea level. In three transects, 10 m×10 m quadrat was used and a total of 18 plots were made. A total of 70 macrofungal taxa were collected. Among them, 56 were identified at the species level, and 14 were generic levels belonging to 26 different families and 12 orders. The highest species-containing family was Russulaceae, with 16 species, whereas the densest species was Mycena sp., comprising 11.8 percent and Cantharellus cibarius was the most frequent species, consisting of 44.44 percent.

CHARACTERIZATION AND NUTRITIONAL ANALYSIS OF CULTIVABLE WILD EDIBLE MUSHROOMS COLLECTED FROM DISTRICT AYODHYA (U.P.), INDIA

Holy city Ayodhya is situated at the bank of river Saryu and has a vast diversity of macrofungi, especially mushroom mycoflora. Some of which are excellently edible and cultivable. Characterization and nutritional composition of four selected cultivable naturally growing mushrooms viz. Agaricus bisporus, Pleurotus ostriatus, Volvariella volvacea and Calocybe indica from different sites of study area (Ayodhya) were evaluated. Macroscopic and microscopic characteristics expressed with natural photographs. The proximate analysis of nutritional values was done by encountering complete mushroom samples that were shade dried, powdered and processed. The macronutrients profiles revealed that the cultivable wild edible mushroom contains protein, carbohydrate, lipid, fiber and ash content ranged from 30.21-34.21%, 22.56-38.39%, 2.35-4.15%, 11.79-23.94% and 7.75-12.97% respectively on dry weight basis. Current study confirms that the selected cultivable wild edible mushrooms are a healthy and good source of food and major alternative source of protein. It also exposed the use of varieties of mushroom in their cultivation practice as well as diet to decrease malnutrition and increase socioeconomic values.

Macrofungal Diversity of Brahakshetra Community Forest, Ghorahi, Dang, Nepal

Fungi are a diverse group of organisms ranging from microscopic to macroscopic mushrooms. Being a major group of decomposers, they are essential for the survival of other organisms in the ecosystem and important for the degradation of organic matter. The main aim of this study was to study the macrofungal diversity of Brahakshetra Community Forest, Ghorahi, Dang. The forest is mainly dominated by Shorea robusta and other associated species. The study was made from June to September 2020. The sampling was done by using a 10×10m quadrate in a line transect and each plot was divided into 5×5m quadrats and samples were collected during the rainy season from different habitats of the forest. Mushrooms were photographed in their natural habitat and preserved in dry and liquid preservation. The specimens were identified by examining their macroscopic and microscopic features, and also by using references from standard literature and websites. In the study, a total of 66 species were recorded under 30 genera belonging to 21 families, and 8 orders. Among them, 65 belong to basidiomycetes and 1 belongs to Ascomycetes. Agaricales was found as the largest order followed by Russulales and Boletales. The diversity indices, the Shannon Weiner index and Simpson diversity index were found to be 3.59 and 0.93 respectively. The result of this study concludes that the Brahakshetra Community Forest was rich in macrofungal diversity, especially ectomycorrhizal. There were positive trends between species richness of macrofungi and environmental variables, i.e., tree canopy, soil pH, soil moisture, and leaf litter which means species increased with increasing these environmental variables.

MACROFUNGAL DIVERSITY IN DIFFERENT VEGETATION COMPOSITIONS IN TEGHARI COMMUNITY FOREST, KAILALI, WEST NEPAL

Macrofungi are high-value forest resources that have functionally significant roles in the forest ecosystem. The macrofungal community of three different vegetation compositions, i.e., Sal (Shorea robusta) Forest, Tropical Deciduous Riverine Forest, and Tropical Evergreen Forest of Teghari Community Forest were investigated. Systematic random sampling was made where 60 plots (10 x 10 m) were laid in all different forest types (20 plots in each). A total of 102 macrofungi species were reported belonging to 36 families. Polyporaceae (17 species) was the largest family followed by Tricholomataceae (13 species) and saprophytic fungi were more frequent than mycorrhizal and parasitic fungi. The tropical evergreen forest was rich in macrofungi (59 species) followed by sal forest (40 species) and tropical deciduous riverine forest (38 species). Macrofungal diversity was directly related to surrounding host species. Similarly, increased soil moisture and canopy cover intensified the abundance of saprophytic fungi. The species richness was increased with increasing organic carbon, canopy, moisture, pH, and litter cover. However, soil nitrogen, phosphorus, and potassium were less significant in affecting species richness. Also, the disturbance was negatively correlated with the species richness of macrofungi. This study highlights the hidden diversity which is necessary for the conservation of macrofungi, to optimize forest ecosystem integrity and resilience against biotic and abiotic agents

Diversity of Basidiomycetous Macrofungi from Mpanga Forest in Mpigi District, Central Uganda

The present study was conducted at Mpanga Research Forest located in Mpigi District, Uganda, during the months of March, April, May and Jun 2020 (for the first rainy season) then in September, October, November, and December 2020 (for the second rainy season) to determine the diversity and distribution of macrofungi as well as their influence by seasonality, and physicochemical properties of the soil. An inventory was carried out through plot sampling and survey which consists of installing three permanent plots of 30 m x 30 m in each of the four selected sites, the soil was also measured in the sample plots. To measure distribution and diversity, abundance, species richness, density, and Simpson's and Shannon's indices were calculated. SPSS version 20 software was used for the significance tests of the diversity parameters between the two rainy seasons and for those of the correlation between the soil factors and the abundance of macrofungi species. A total of 120 species of basidiomycetous macrofungi distributed in 53 genera and 22 families were recorded. The dominant genus was Psathyrella followed by Marasmius belonging to the most dominant families (Coprinaceae and Marasmiaceae), and the most dominant orders (Agaricales and Tricholomatales). During the two rainy seasons, the majority of the species that have been collected belong to the group of saprophytes. Macrofungi species collected during the second rainy season were more abundant and diverse than those collected during the first rainy season. Among the physicochemical properties of the soil, pH, calcium, potassium, nitrogen, organic carbon, phosphorus, clay, sand and organic matter were significantly correlated with the abundance of macrofungal species. The results of this study provided basic information on the diversity of macrofungi in Mpanga forest, it can be a point of reference for further research to study the evolution of macrofungal biodiversity in this forest.