Part Of Machinery Research Articles

Deubiquitinases in cell death and inflammation.

Apoptosis, pyroptosis, and necroptosis are distinct forms of programmed cell death that eliminate infected, damaged, or obsolete cells. Many proteins that regulate or are a part of the cell death machinery undergo ubiquitination, a post-translational modification made by ubiquitin ligases that modulates protein abundance, localization, and/or activity. For example, some ubiquitin chains target proteins for degradation, while others function as scaffolds for the assembly of signaling complexes. Deubiquitinases (DUBs) are the proteases that counteract ubiquitin ligases by cleaving ubiquitin from their protein substrates. Here, we review the DUBs that have been found to suppress or promote apoptosis, pyroptosis, or necroptosis.

Deposition of carbonitride titanium coatings by magnetron sputtering and its effect on tribo-mechanical properties

Metal parts in machinery often fail as a result of damage caused by wear and tear, resulting in the loss of functionality of the products. Thin film solid nitride coatings are used to improve the wear resistance and service life of parts and are considered to be effective. The article presents a brief overview of modern literature in the field of obtaining wear resistant coatings of titanium carbonitride by using magnetron sputtering. The review presents a detailed assessment of the scientific results obtained depending on the deposition parameters and the conditions for obtaining coatings. The results of the coefficient of friction, wear rate of the coating and counterbody, nanohardness and adhesion force of coatings obtained by magnetron sputtering and its modifications are shown. The influence of alloying elements on the mechanical and tribological properties of titanium carbonitride coatings is considered. Recent advances in the production of titanium carbonitride coatings with improved wear characteristics are discussed.

Determination of abrasion resistance of Fe28Cr5C1Mn coating applied to 30MnB5 boron alloy cultivator blades via electric arc spray

The poor abrasive wear behavior of agricultural machinery affects the farmers all over the world, rendering their activities more inefficient and negatively affecting their productivity. Therefore, innovation in the manufacture of soil cultivation machinery and the selection of wear-resistant materials has been of great importance. Recently, boron alloys, in particular, have been used in the manufacture of machinery parts that work the soil. The aim of this was to investigate the behavior of the blades of the machines widely used in soil cultivation in agricultural production following a surface treatment technique. For this purpose, the blades made of 30MnB5, a material widely used in the production of cultivator blades, were used as substrate samples in the trials as well as blades of the same substrate coated with Fe28Cr5C1Mn alloy wire via the electric arc spray technique. The morphologies and structures of the uncoated and coated samples were comprehensively analyzed by light microscopy, scanning electron microscopy, energy dispersive spectrometry and X-ray diffraction. In addition, their microhardness, porosity, and surface roughness properties were assessed separately before and after plowing. These prepared blades were tested under in the same conditions by plowing the same field at two different soil moisture. Although the weight loss was measured as 9.4 g and 5.4 g in the uncoated and in the coated sample at 9% moisture level, respectively, the weight losses were at 14% humidity level were 10.6 g and 5.9 g in the uncoated and coated samples, respectively. Although the amount of wear increased with increasing humidity levels in both groups, the mass wear loss in cultivator blades coated with Fe25Cr5C1Mn alloy wire was found to be lower in all trials. The results suggest that coating the cultivator blades using electric arc spray would increase agricultural productivity and reduce factors that cause environmental pollution.

A genetic screen for proteins involved in Non‐Stop mRNA decay in C. elegans

Non‐stop mRNAs, which lack a stop codon due to mutation or errors in mRNA transcription or processing, serve as templates for production of aberrant and potentially toxic protein products. Ultimately, translation of non‐stop mRNAs leads to a failure in normal translation termination and ribosome collisions, which in eukaryotes initiates ubiquitin‐dependent degradation of the protein product and Non‐Stop Decay (NSD) of the problematic mRNA. Ribosome collisions in yeast are linked to activation of cellular stress responses and failure to resolve stalled ribosomes leads to neurodegeneration in mice, highlighting the biological importance of mRNA quality control.In the nematode Caenorhabditis elegans, loss of the core NSD components pelo‐1 and skih‐2 leads to temperature‐sensitive sterility, as does the loss of endogenous siRNAs that are triggered by NSD at some loci. To further probe the link between NSD and the biology of germ cells, we have used an RNAi approach to screen for temperature‐sensitive reduction in brood size for the C. elegans orthologs of several proteins known to be part of the NSD machinery in other organisms. Preliminary results have identified 2 NSD‐associated genes with conditionally reduced brood sizes. We will present progress on examining the requirement of these genes for Non‐Stop decay in C. elegans, as well as investigation of their specific contributions to the biology of the germ line.

Tungsten carbide material tribology and circular economy relationship in polymer and composites industries

The implementation of the circular economy concept in industries demands experimental and innovative investigations. The set of strategies and paradigms that express the theoretical base of the circular economy plays a vital role in the enhancement of the quality and performance of polymer and composite materials. In this article, tungsten carbide material is advanced for tribological investigations. The scanning electron microscope and an optical and mechanical profilometer were used for the analysis of cotton polymer and tungsten carbide ball surfaces. A newly developed tribometry technique was introduced to investigate the coefficient of friction, wear, and deformation. The cotton surface was found damaged and rough. The tungsten carbide balls showed low roughness and higher hardness. The average surface roughness parameters Ra, Rz, and Rp of tungsten carbide balls were 0.10, 0.15, and 0.20, respectively. The average friction constant values were found to be 0.12–0.15 in the perpendicular direction and 0.11–0.17 in the parallel direction. Reciprocation distance increment has been used for industrial optimization. The coefficient of friction remained constant and slightly deformed cotton polymer. Based on the friction values, deformation, wear, and morphology evaluations, tungsten carbide ceramic materials could be used operationally for surface alterations of industrial machinery parts. The results could also enhance the quality and performance of polymer and composite products.

Robot Remote Monitoring and Fault Diagnosis Based on Industrial Internet of Things

In order to realize the remote monitoring of robots, a remote monitoring platform for industrial robots is designed based on the browser/server (B/S) architecture. Through this platform, users can check the real-time running parameters and running status of robots in any place with network. The Industrial Internet of Things scheme of remote platform system is proposed to adopt three-layer structure: on-site “perception layer,” information “transmission layer,” and remote data service center. The data acquisition controller of the whole system and the core part of the sensor layer is designed. The data acquisition controller adopts the embedded platform design, which can be directly connected with the control cabinet of the industrial robot to read the running status of the robot in real time, monitor the alarm and warning data, and it is transmitted to the local server and remote service center in the first time. At the same time, the robot can receive the control command of the server for remote debugging and fault maintenance. Aiming at the data model of industrial machinery parts, a fault prediction method based on BP neural network algorithm is proposed. According to the needs of the target algorithm and the analysis of the measurement results, an attempt is made to obtain a more feasible fault diagnosis and early warning method. Through the remote monitoring system, fault early warning and corresponding troubleshooting methods are realized.

PRODUCIBILITY OF FUNCTIONALLY GRADED AlB2/Al COMPOSITE MATERIAL

This study is intended to investigate producibility of functionally graded AlB2/Al composites which are used in the manufacture of ship machinery parts. AlB2 particles have been spontaneously formed in liquid matrix as reinforcement. A semi-solid composite (Al(l) - AlB2(s)) prepared at 850°C was solidified under a centrifugal force to grade functionally. The properties of composite materials such as hardness and microstructure have been examined. This research provided the following findings, AlB2 particles can be successfully synthesized with in situ reaction technique in molten aluminum. It was determined that the hardness value of the composites increases with increasing AlB2 reinforcement content within matrix. Functionally graded AlB2/Al composite materials can be successfully produced with centrifugal casting technique. It is also determined that AlB2 reinforcement ratio of the composite materials ranged from 0 to 7 vol. %. In addition, it was observed that the hardness values of the composites increased with the addition of reinforcement ratio.

Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila

ABSTRACT The endolysosomal system not only is an integral part of the cellular catabolic machinery that processes and recycles nutrients for synthesis of biomaterials, but also acts as signaling hub to sense and coordinate the energy state of cells with growth and differentiation. Lysosomal dysfunction adversely influences vesicular transport-dependent macromolecular degradation and thus causes serious problems for human health. In mammalian cells, loss of the lysosome associated membrane proteins LAMP1 and LAMP2 strongly affects autophagy and cholesterol trafficking. Here we show that the previously uncharacterized Drosophila Lamp1 is a bona fide ortholog of vertebrate LAMP1 and LAMP2. Surprisingly and in contrast to lamp1 lamp2 double-mutant mice, Drosophila Lamp1 is not required for viability or autophagy, suggesting that fly and vertebrate LAMP proteins acquired distinct functions, or that autophagy defects in lamp1 lamp2 mutants may have indirect causes. However, Lamp1 deficiency results in an increase in the number of acidic organelles in flies. Furthermore, we find that Lamp1 mutant larvae have defects in lipid metabolism as they show elevated levels of sterols and diacylglycerols (DAGs). Because DAGs are the main lipid species used for transport through the hemolymph (blood) in insects, our results indicate broader functions of Lamp1 in lipid transport. Our findings make Drosophila an ideal model to study the role of LAMP proteins in lipid assimilation without the confounding effects of their storage and without interfering with autophagic processes. Abbreviations: aa: amino acid; AL: autolysosome; AP: autophagosome; APGL: autophagolysosome; AV: autophagic vacuole (i.e. AP and APGL/AL); AVi: early/initial autophagic vacuoles; AVd: late/degradative autophagic vacuoles; Atg: autophagy-related; CMA: chaperone-mediated autophagy; Cnx99A: Calnexin 99A; DAG: diacylglycerol; eMI: endosomal microautophagy; ESCRT: endosomal sorting complexes required for transport; FB: fat body; HDL: high-density lipoprotein; Hrs: Hepatocyte growth factor regulated tyrosine kinase substrate; LAMP: lysosomal associated membrane protein; LD: lipid droplet; LDL: low-density lipoprotein; Lpp: lipophorin; LTP: Lipid transfer particle; LTR: LysoTracker Red; MA: macroautophagy; MCC: Manders colocalization coefficient; MEF: mouse embryonic fibroblast MTORC: mechanistic target of rapamycin kinase complex; PV: parasitophorous vacuole; SNARE: soluble N-ethylmaleimide sensitive factor attachment protein receptor; Snap: Synaptosomal-associated protein; st: starved; TAG: triacylglycerol; TEM: transmission electron microscopy; TFEB/Mitf: transcription factor EB; TM: transmembrane domain; tub: tubulin; UTR: untranslated region.

Automatic recognition method of installation errors of metallurgical machinery parts based on neural network

Abstract The installation error of metallurgical machinery parts is one of the common sources of errors in mechanical equipment. Because the installation error of different parts has different influences on different mechanical equipment, a simple linear formula cannot be used to identify the installation error. In the past, the manual recognition method and the touch recognition method lack of error information analysis, which leads to inaccurate recognition results. To improve the problem, an automatic recognition method based on the neural network for metallurgical machinery parts installation error is proposed. According to the principle of automatic recognition of installation error based on the neural network, the nonlinear relation matrix between layers of the neural network is established. The operating state parameters of mechanical equipment are calculated, and the time series of the parameters are divided into several segments averagely. Based on the recognition algorithm, the inspection steps of depth, perpendicularity and center position of reserved hole, base board construction, short-circuit motor line and terminal installation, center mark board, and reference point installation are designed. The experimental results show that the recall rate of the proposed method is 97.66%, and the maximum absolute deviation is 0.09. The experimental data verify the feasibility of the proposed method.

The omnipresence of autonomic modulation in health and disease.

The Autonomic Nervous System (ANS) is a critical part of the homeostatic machinery with both central and peripheral components. However, little is known about the integration of these components and their joint role in the maintenance of health and in allostatic derailments leading to somatic and/or neuropsychiatric (co)morbidity. Based on a comprehensive literature search on the ANS neuroanatomy we dissect the complex integration of the ANS: (1) First we summarize Stress and Homeostatic Equilibrium - elucidating the responsivity of the ANS to stressors; (2) Second we describe the overall process of how the ANS is involved in Adaptation and Maladaptation to Stress; (3) In the third section the ANS is hierarchically partitioned into the peripheral/spinal, brainstem, subcortical and cortical components of the nervous system. We utilize this anatomical basis to define a model of autonomic integration. (4) Finally, we deploy the model to describe human ANS involvement in (a) Hypofunctional and (b) Hyperfunctional states providing examples in the healthy state and in clinical conditions.

Mechanical, Morphological and Water absorption properties of Polypropylene based Composites

Polyethylene compounds are used instead of PE in many applications to improve the mechanical properties of PE (such as tensile strength, tensile modulus and tensile strength). Different types of combinations can be used to enhance these properties. Fibers are considered to be effective reinforcing fillers for PE. Plastic compound? Not all plastics are compounds. In fact, most plastics used in toys, water bottles and other familiar items are not composite. They are clean plastics. Polypropylene is not as solid as polyethylene. Polypropylene is harder than polyethylene and is resistant to chemicals and organic solvents. Polypropylene is pure, non-stretchable and much harder than polyethylene, commonly called fiberglass, in fact such a compound Also known As for glass-fiber reinforced plastic (GFRP), this is Specific to material properties such as tensile strength Made in different formats for permutations, stiffness, compressive strength and thermal expansion and different price points. For one, plastic deck boards are completely synthetic. It is considered safe in all plastics; it is a strong heat-resistant plastic. Due to its high heat tolerance, it is unlikely to leak even when exposed to hot or cold water. It is allowed to use with storage of food and beverages. Polypropylene (PP) is the most common in the world Is one of the thermoplastics used. Polypropylene plastic packaging, machinery and Plastic parts and threads for equipment and also used for textiles. Some plastic, BPA or other harmful chemicals in our body Or it could badly affect the world we live in. Polypropylene, a complex plastic, is common Considered safe for humans and these common ingredients are often the same Are used for products, but not theirs What are the differences? Polyester and polypropylene The main difference between the two is polyester Polypropylene is more water resistant than, It works well for athletic wear Is a quick-drying cloth. Plastic is a polymer with a large molecular weight. Polypropylene is an example of a plastic polymer. The main difference between polypropylene and plastic is that we can make crystal clear material out of polypropylene, while plastic material is generally not clear.

Determination of the peak force of a potato tuber at the rod collision by means of finite element analysis

ABSTRACT Potato is exposed to different types of impact loads during harvest, sorting, and transportation. The large impact force between potato and rod-shaped machinery parts causes the potato damage and brings economic losses for the food industry. To obtain the impact force and its influencing laws between potato and rod, both theoretical analysis and finite element analysis (FEA) were used. By defining the impact force coefficient (K), a mechanical model between the impact peak force ( F max ), potato mass (m), and drop height (h) was established. The FEA virtual experiments showed that F max was increased linearly with the increase in m, h, and m h . When the potato mass was from 0.2 kg to 0.35 kg and the drop height was from 0.2 m to 0.3 m, the regression equation between F max and m h had a similar structure to the mechanical model, which verified the theoretical analysis and allowed the assessment of potato impact force. The results of this article can contribute to predicting the impact force of potato and solving other problems of solid-like agricultural materials related to impact.

Development of anti-corrosion properties using ternary electrodeposited Zn–Ni–Co alloys from acidic electrolyte for automobile applications

ABSTRACT In the present work, the authors designed a deposition process and prepared ternary Zn–Ni–Co alloy material over a mild steel substrate via direct current deposition using an acidic electrolyte for the development of good surface quality and corrosion resistance properties in metal finishing uses. The quality of coatings has been optimised, through implementation of different experimental conditions and parameters, respectively. The prepared specimens have been subjected to X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDS). The XRD pattern revealed highly crystalline properties and FESEM revealed a bright and uniform deposit distribution with an aggregated spherical structure. Electrochemical studies through Tafel polarisation testing and electrochemical impedance spectroscopy (EIS) were carried out in 3.5% NaCl solution. The Tafel analysis revealed that Zn–Ni–Co alloy deposited with the highest corrosion resistance property was observed at a practical currect density of 1 A dm−2. Thus, it can be possible to produce coated material for good corrosion resistance for machinery parts in automobile industries.

Experimental Studies on Geometrical Accuracy During Single-Point Incremental Forming of Inconel 625 Superalloy

The single-point incremental forming has been globally accepted as an advanced method of sheet metal forming due to its rapid prototyping and economic benefits. The process has sensitively released the practice of using expensive dies, making it suitable for manufacturing custom-built products in tiny batches. Further, it is getting acceptance in producing parts of shabby machinery. In recent years, superalloys have become the most commonly used materials in the transportation, automotive, aeronautics and marine industries due to their fundamental and structural applications. The input parameters considered in this study are step size, feed rate and tool spindle speed. The effects of all three process parameters on the geometrical accuracy of Inconel 625 superalloy conical parts formed by the single-point incremental forming process are considered in this work. The deviations in roundness, concentricity, half-cone angle and straightness from the target values were considered as response parameters to measure the accuracy. The results showed that the part accuracy could be enhanced by using minimum step size, feed rate and maximum tool spindle speed.

Corporate governance and the use of external assurance for integrated reports

AbstractResearch Question/IssueThis paper investigates the relationship between the use of external assurance for testing integrated reports (ESG assurance) and firm‐level governance features: the board of directors, the audit and/or risk committee, and the internal audit department. Data are collected from South Africa where integrated reporting and corporate governance practices are mature and listed companies have had more time to implement ESG assurance than in other countries.Research Findings/InsightsMonitoring attributes of boards of directors promotes the use of ESG assurance which provide both limited (moderate) and reasonable (high) assurance. The monitoring attributes of the audit and risk committees limit the use of limited assurance but are associated with the greater use of reasonable assurance. In contrast, internal audit functions are not affecting the use of ESG assurance.Theoretical/Academic ImplicationsThe study provides one of the first accounts of how firm‐level governance promotes or reduces the use of external assurance in an integrated reporting context. The research also frames ESG assurance as part of the broader corporate governance machinery rather than seeing assurance and governance as separate issues.Practitioner/Policy ImplicationsOverall, the findings suggest that ESG assurance is an important part of a combined assurance model. As those charged with governance become more proactive in ensuring the credibility of their organizations' corporate reports, they not only choose to appoint an external assuror but also rely on more extensive testing designed to provide higher levels of assurance.

Optogenetic delivery of single molecules to the plasma membrane

Single molecule microscopy is a powerful tool for the investigation of functional events at the plasma membrane. With state of the art microscopy, changes is membrane mobility can be correlated to cellular events or structures and offer quantitative data that from ensemble methods is impossible to yield. Especially the investigation of single molecules as part of signalling machinery promises insight into stoichiometry and dynamics of signalling complexes involve in many key membrane processes in biology.

DNA-induced spatial entrapment of general transcription machinery can stabilize gene expression in a nondividing cell

An important characteristic of cell differentiation is its stability. Only rarely do cells or their stem cell progenitors change their differentiation pathway. If they do, it is often accompanied by a malfunction such as cancer. A mechanistic understanding of the stability of differentiated states would allow better prospects of alleviating the malfunctioning. However, such complete information is yet elusive. Earlier experiments performed in Xenopus oocytes to address this question suggest that a cell may maintain its gene expression by prolonged binding of cell type-specific transcription factors. Here, using DNA competition experiments, we show that the stability of gene expression in a nondividing cell could be caused by the local entrapment of part of the general transcription machinery in transcriptionally active regions. Strikingly, we found that transcriptionally active and silent forms of the same DNA template can stably coexist within the same nucleus. Both DNA templates are associated with the gene-specific transcription factor Ascl1, the core factor TBP2, and the polymerase II (Pol-II) ser5 C-terminal domain (CTD) phosphorylated form, while Pol-II ser2 CTD phosphorylation is restricted to the transcriptionally dominant template. We discover that the active and silent DNA forms are physically separated in the oocyte nucleus through partition into liquid-liquid phase-separated condensates. Altogether, our study proposes a mechanism of transcriptional regulation involving a spatial entrapment of general transcription machinery components to stabilize the active form of a gene in a nondividing cell.

The role of photosynthesis related pigments in light harvesting, photoprotection and enhancement of photosynthetic yield in planta.

Photosynthetic pigments are an integral and vital part of all photosynthetic machinery and are present in different types and abundances throughout the photosynthetic apparatus. Chlorophyll, carotenoids and phycobilins are the prime photosynthetic pigments which facilitate efficient light absorption in plants, algae, and cyanobacteria. The chlorophyll family plays a vital role in light harvesting by absorbing light at different wavelengths and allowing photosynthetic organisms to adapt to different environments, either in the long-term or during transient changes in light. Carotenoids play diverse roles in photosynthesis, including light capture and as crucial antioxidants to reduce photodamage and photoinhibition. In the marine habitat, phycobilins capture a wide spectrum of light and have allowed cyanobacteria and red algae to colonise deep waters where other frequencies of light are attenuated by the water column. In this review, we discuss the potential strategies that photosynthetic pigments provide, coupled with development of molecular biological techniques, to improve crop yields through enhanced light harvesting, increased photoprotection and improved photosynthetic efficiency.

Failure to Detect Mutations in U2AF1 due to Changes in the GRCh38 Reference Sequence

The U2AF1 gene is a core part of mRNA splicing machinery and frequently contains somatic mutations that contribute to oncogenesis in myelodysplastic syndrome, acute myeloid leukemia, and other cancers. A change introduced in the GRCh38 version of the human reference build prevents detection of mutations in this gene, and others, by variant calling pipelines. This study describes the problem in detail and shows that a modified GRCh38 reference build with unchanged coordinates can be used to ameliorate the issue.

A CNN-ABiGRU method for Gearbox Fault Diagnosis

As a key part of modern industrial machinery, there has been a lot of fault diagnosis methods for gearbox. However, traditional fault diagnosis methods suffer from dependence on prior knowledge. This paper proposed an end-to-end method based on convolutional neural network (CNN), Bidirectional gated recurrent unit (BiGRU), and Attention Mechanism. Among them, the application of BiGRU not only made perfect use of the time sequence of signal, but also saved computing resources more than the same type of networks because of the low amount of calculation. In order to verify the effectiveness and generalization performance of the proposed method, experiments are carried out on two datasets, and the accuracy is calculated by the ten-fold crossvalidation. Compared with the existing fault diagnosis methods, the experimental results show that the proposed model has higher accuracy.