This article describes the issue of geometric accuracy of large machine tools. The operation and maintenance of large CNC machine tools is usually affected by a number of aspects related to large workpieces and spaces at the machine installation site. It is the size of the working space and the large dimensions of the production halls that very often have a major impact on the operation of these machines. Large workpieces remain clamped on the machine for weeks and are therefore affected by the changing environment. The presented study captures and presents changes in the ambient conditions of a large CNC machine tool, including its effects on the geometric accuracy of the machine. The first part of the article describes the geometric errors of the assessed machine. The second part also presents selected ambient conditions of the machine with the resulting measured data. The last part of the presented study summarizes the conclusions describing the influence of ambient conditions on the geometric accuracy of the machine.