Highlights Laser diffraction analysis of soil particle size distribution is uniquely suited for active turf racetracks. Eight standard sampling locations are shown to be sufficient for characterizing a racing surface. Differences between the three archetypes of turfgrass racing surfaces were significant using laser diffraction. There is a need for consensus on the proper handling of organic content in samples. Abstract. Significant research has focused on North American dirt and synthetic Thoroughbred racing surfaces. Turfgrass racing surfaces have received less consideration. Basic information, including climate and turfgrass species, can be documented relatively easily. However, a key characteristic, the particle size distribution of the growing medium, is not readily available for turf tracks. Particle size distribution and the deviation from nominal values are important to infiltration rate, shear strength, and turf health, as well as being critical for the selection of top-dressing and divot repair sand. The primary difficulty with obtaining the particle size distribution is the relatively large quantity of material required for traditional sedimentation test methods. Sampling an active racing surface could present a risk to the horses and riders. Laser diffraction testing methods present an opportunity to use much smaller samples. The use of smaller samples introduces new questions about the ability of a small sample to represent a large area, such as a racetrack. Tests were carried out with high resolution sampling at one racetrack. By sampling a large number of locations, 96 locations on a single racetrack, the variability of the track could be evaluated, and an eight-sample protocol was developed. Using the eight-location protocol, 22 additional turf racetracks throughout North America were sampled. A total of 23 turf racetracks were tested, representing all three of the designs used for North American turf racetracks. By looking at the three different track designs: engineered profile, engineered profile with fiber, and native soil, appropriate testing parameters and measurements were identified. While the primary objective was to understand turf racetracks, this unique data set also provided a method to investigate the applicability of laser diffraction for the analysis of soil samples. Mineralogy and organic content had previously been identified as important in the measurement of particle size distribution using laser diffraction. Mineralogy and organic content were determined for samples from each surface using X-ray diffraction (XRD) and loss on ignition. The PSD of the three types of turfgrass horse racing surfaces showed significant differences between native soil (N), engineered surfaces without synthetic fibers (EWOF), and engineered surfaces with synthetic fibers (EWF). These basic design descriptions were also found to be sufficient for making reasonable estimates of the settings used in the machine configuration and sample preparation. A single refractive index was used for the entire range of samples in this group; however, the sample quantity tested was different for the three different types of track designs. Keywords: Horse, Laser diffraction, Racing, Soil, Thoroughbred, Turf, Turfgrass.