Lysine Content Research Articles

Compositions of gilthead sea bream (Sparus aurata Linnaeus, 1758) from different culture systems

The aim of the present study was to investigate the effects of seasons (summer and winter) on the proximate, amino acid and fatty acid compositions of gilthead sea bream fish fed with commercial diets in sea cages and earthen ponds in the Aegean Sea in Türkiye. In general, the nutrient composition of the diets met the nutritional needs of the fish. Proximate analysis of fish fillets revealed that fish reared in earthen ponds had the lowest moisture and highest lipid content in the winter season (p < 0.05). Although fillet protein contents were comparatively higher in the winter (p < 0.05), there was no significant difference between systems in the same season (p > 0.05). Growing gilthead sea bream in marine cages produced fillets with high contents of the essential fatty acids (EFA) eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). On the other hand, the 18:1n-9 and 18:3n-3 contents of fish reared in earthen ponds increased by 25.64 and 18.80% respectively, compared to those in marine cages. Fillet content of total n-3 fatty acid were comparatively higher (p < 0.05) in gilthead sea bream from marine cages, and this increase was even more evident in the winter months. The n-3/n-6 ratios of fillets were significantly high in gilthead sea bream from marine cages in the summer. The levels of the essential amino acids (EAAs) arginine and leucine were higher in fillets from both systems in the winter. On the other hand, fillet contents of lysine were higher in the summer. In general, the total EAA content of fillets was higher in fish from the sea cages in summer. EAA/NEAA ratios in both systems in summer and winter were found to be at good levels in terms of fish fillet quality. Fillet contents of EFA and EAA from both systems and seasons were found to be good for human consumption and will have positive effects on human nutrition. In conclusion, commercial diets used in feeding gilthead sea bream improved the proximate, fatty acid and amino acid compositions of fillets to a good extent. Different seasons and culture systems may affect the nutritional composition of fish fillets, especially on fillet EFA contents. Results of fillet samples in marine cages and earthen ponds in summer and winter showed that the fish were of good quality for human health. Evaluation of the results in the present study shows that that gilthead sea bream can also be successfully cultured in earthen ponds in areas unsuitable for agriculture.

Inhibition of advanced glycation end-products in frozen-thawed and reheated pork by pigskin gelatin hydrolysates.

This study investigated the effects of pigskin gelatin hydrolysates (PGH, 4 %) as a cryoprotectant on the formation of advanced glycation end-products (AGEs) in pre-heated pork subjected to freeze-thaw cycles and subsequent reheating. During the freeze-thaw process, PGH significantly mitigated the increase in α-dicarbonyl precursors (α-DPs) and AGEs compared to the control group. Specifically, the levels of glyoxal and methylglyoxal decreased by 4.26 % and 6.12 %, respectively, although this inhibition was not statistically significant (P > 0.05). The concentrations of Nε-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine (CEL) content in the PGH-treated group were 2.86 %-33.19 % and 4.33 %-24.02 % lower than those in the control group, respectively. After reheating (100 °C, 10 min), AGEs levels increased by 1.94 % to 123.21 %, while the levels of glyoxal and methylglyoxal decreased by 17.00 % and 15.24 %, respectively. The addition of PGH significantly reduced AGEs formation after reheating compared to the control (P < 0.01). These results suggest that PGH is effective as a cryoprotectant, inhibiting the formation of harmful AGEs during freeze-thaw cycles and subsequent reheating of pre-heated pork.

RESEARCH ON THE INCREASE OF TRITICALE PRODUCTION THROUGH THE MODIFICATION OF THE SOWING RATE AND SUPPLEMENTARY FERTILIZATION, IN THE CLIMATE CONDITIONS OF 2023-2024 IN NORTH-WESTERN ROMANIA

Triticale are known around the world for their properties, borrowed from the genera Triticum aestivum and Secale cereale. They have a high production capacity and a high content of protein and lysine, show a very good resistance to abiotic and biotic stress, and have a high capacity to capitalize on low fertility and acid lands, lands where wheat cannot be cultivated. Along with the advancement of technology and science, the use of triticale grains and plants has seen new fields of use. In addition to feeding animals in different forms, triticale production is also used in the bioethanol, cellulose industry, in the bakery industry and in the manufacture of biscuits, beer, in the food packaging industry, in human medicine and in diets, aquaculture, etc. Due to the expansion of the use of this culture, the need to obtain higher productions is absolutely necessary. In the current study, which took place at the Livada Agricultural Research and Development Station, in Satu-Mare county, northwest Romania, in the period 2023-2024. It was based on the testing of 4 varieties of triticale that were sown in different sowing rates and that received different fertilization schemes, the control version was based on chemical fertilization at the soil level, with complex fertilizer in the fall, and with Nitrolime in the spring vegetation. The experiment was placed under the method of subdivided plots, having three experimental factors; the variety, with 4 graduations, the fertilization, with 3 graduations, the seed size, with 3 graduations. In the pedoclimatic conditions of the reference area, it was found that the Utrifun variety was the most productive, it reacted favorably to the additional fertilization with foliar fertilizer and signed at a plot of 550 seeds/m2 , obtaining productions of over 11.000 kg/ha, in in the case of additional fertilization and with biostimulator, the production started to decrease, however, Utrifun fertilized only in the soil managed to produce over 10.000 kg/ha. This was followed by the Zvelt variety, the latter being Tulnic. These results were obtained under conditions specific to the 2023-2024 period, and in the following years it will be established whether additional fertilization with foliar fertilizers ensures increases in production and whether the additional application of a biostimulator leads to increased production.

Molecular Diversity of Commercially Available Edible Oyster Mushroom by SSR Marker

Oyster mushroom (Pleurotus species) are commercially cultivated worldwide. Oyster mushroom naturally found in rotten wood or agricultural lignocellulosic residues as saprophytic in nature. Due to the good amount of protein with low carbohydrates people prefer it in their daily diet. It can be alternate option of vegan protein with high lysine content up to 6-8% essential amino acid from fresh and dried sample. Our study aims to evaluated that availability of Simple sequence Repeat (SSR) PCR based marker for genetic diversity analysis on five different oyster mushrooms were also identified on the basis of polymorphism. The dendrogram obtained from UPGMA cluster analysis of Jaccard’s similarity values based on 7 SSR markers grouped 5 strains into two clusters species wise (P. djamor, P. florida, P. ostreatus, P. pulmonarius and P. sajor-caju) which confirm the reliability of PCR based molecular markers. diversity analysis also confirmed the grouping of genotypes as they were present in same group as revealed by SSR markers.

Коллаген, гидролизованный из сырья маралов: технология получения и биохимический состав

Collagen has a complex beneficial effect on human health, which makes it a popular component in various therapeutic diets. Deer antlers are a promising source of collagen. It has been used in traditional Chinese medicine for more than 20 centuries as an additive that supports the musculoskeletal system. The article describes the effect of extraction technology on the amino acid and biochemical composition of collagen obtained from the Altai wapiti, or maral (Cervus Canadensis). The research featured hydrolysates obtained from ground skin and tendons of ten marals. The mass fraction of collagen was determined by the concentration of oxyproline. The yield of dry solids was calculated in line with State Standard GOST 316402012. The method of atomic adsorption spectroscopy made it possible to calculate the mass concentration of macro- and microelements. Amine nitrogen was detected by formol titration while the general amino acid composition was studied using the method of high-performance liquid chromatography. The list of amino acids included glycine (14.36 g/100g), proline (8.87 g/100g), and oxyproline (7.83 g/100 g). Their concentration depended on the production technology. The content of arginine and lysine increased 4–5 times during fermentation and 1.5–2.3 times during high-temperature extraction of oxyproline, glutamic acid, and threonine. A step-by-step hydrolysis protocol with fermentation and high-temperature extraction provided the maximal yield of dry solids and amino acids. In this study, the collagen hydrolyzed from maral skin and tendons was rich in glycine, proline, and oxyproline, which makes it a prospective additive to be used in products that lack these amino acids. The effect of hydrolyzed maral collagen on the human body needs further research

Quality Assessment of Different Agricultural Produce under Organic as Well as Inorganic Production System: A Review

The urgent need to grow more food to feed the growing population has led to intensive farming with a lot of negative impacts on soil health as well as the ecosystem. To mitigate the negative impacts of conventional farming, the concept of organic farming has resurfaced. However, separate study of conventional and organic farming does not give a comparative assessment between two production system. The compensation of yield loss under organic farming could only be overlooked through enhancing the quality of produce. Good quality produce is just as important as quantity, yet quantity is typically prioritized. There is a myth that organically grown produce is superior and tastier than conventionally grown produce, yet this isn’t always the case. So, it’s high time to screen out the specific nutrient/ vitamin or any other quality parameter that can be enhanced through organic farming. So that agro-technique for organic fortification can be developed for the future researchers. Almost 71 research experiment were carefully studied and tried to assess the impact on organic farming on quality of produce. In developed and developing countries the area is increasing with an average growth rate of 20-25 %. Organically grown produce enhanced the mineral content especially phosphorous, and iron in rice, increased the protein content of maize by 8 to 19%, lysine content in wheat by 25-30%, ash and crude fat content in millets and oats and many more. Besides, improving physical appearances such as kernel length, elongation ratio of cereals, the cooking time has been greatly reduced in organic than inorganic production system. Even, the crude protein and oil contents greatly increased in the oilseed crops in organic production system over inorganic. However, variable response was reported in case of tuber crops, as quality parameters as well as organoleptic taste between conventional as well as organic farming system remained insignificant. However, few researchers reported significant increase in tuber dry matter content (20.92%) as well as quality traits for processing of tubers under organic farming system. Likewise, the oleoresin (5.26%) and essential oil (3.96%) contents of zinger rhizomes and curcumin (5.3%) and essential oil contents (4.2%) of turmeric greatly increased in organic production system over inorganic.

Different Correlation Patterns Between Circulating Amino Acids and Body Temperature in Fibromyalgia Syndrome: A Cross-Sectional Study.

The aim of this study was to analyze the association between circulating amino acids and central and peripheral body temperature in subjects with and without fibromyalgia syndrome (FMS). A total of 47 patients with FMS and 59 healthy subjects were included in the study. The concentration of amino acids was determined in serum samples using a fluorimeter coupled with a high-performance liquid chromatography system. An infrared thermography camera was used to estimate peripheral hand temperatures. The core temperature of the body was estimated using an infrared thermometer, which was applied to the axillary and tympanic areas. Correlations between several thermographic variables of the hands and tryptophan, methionine, 3-methylhistidine, histidine, glutamic acid, and tyrosine were identified exclusively within the FMS group. In contrast, correlations between aminoadipic acid and serine and thermographic variables were observed only in the healthy control group. The concentrations of asparagine and lysine correlated with thermographic variables in both groups. The essential amino acid leucine was found to correlate with axillary temperature in FMS patients. However, it should be noted that the observed associations between aminoadipic acid and tryptophan blood concentrations and axillary temperature were limited to the control group. Several correlations were identified between circulating amino acids and different body temperatures in both healthy controls and patients with FMS. However, the correlation pattern differs significantly between FMS patients and healthy controls. These findings suggest the possibility of a change in the function of several amino acids in the thermoregulatory process in patients with FMS.

Process Optimization of Assorted Nutraceutical Biscuits for Improved Protein Accessibility

Background: Assortment of Cereal-legume flour has been gaining attention nowadays for being a promising meat analogue for vegetarian population. Biscuits containing defatted soya flour (DSF), wheat flour (WF) and nutraceutical ingredients were developed for improved protein quality. Methods: T1, T2 and T3 were standardized at 60, 50 and 40% DSF levels to WF against T0 (100% WF). T3 was finalized for assorted nutraceutical biscuits (T4 and T5) and half portion of DSF was replaced with nutraceutical ingredients. Treatments and raw ingredients were analyzed for trypsin inhibitor, amino acid content and in vitro protein digestibility. Result: T4 was the highly acceptable treatment. A significantly (p less than 0.05) higher lysine and methionine contents were found in assorted nutraceutical biscuits than the control samples. In vitro protein digestibility was also higher in T4 and T5. Diverse nutraceuticals can be used in assortment of value added biscuits to obtain quality protein characteristics.

Solid-state fermentation of corn wet distiller grains and wheat bran with Trichoderma reesei and Candida utilis for improving feed value.

Solid-state fermentation is one of the most effective methods for the high-value utilization of agro-industrial by-products. Co-fermentation of wet distiller grains and agricultural waste is an effective way to mitigate the feed shortage caused by corn consumption for bioethanol. It is still challenging to convert wet distiller grains and wheat bran to easily accessible carbon sources and adjust the balanced proportion of amino acids together by fermentation. Fermentation time, strain ratio, and the addition of ammonium sulfate have been verified to be the important factors influencing the symbiosis of Trichoderma reesei (T. reesei) and Candida utilis (C. utilis) in a mixed system of wet distiller grains and wheat bran. The optimum conditions were fermentation for 8 days, 2:1 (T. reesei: C. utilis) strain ratio, and addition of 4% ammonium sulfate. After fermentation, the cellulose degradation proportion reached 39.1%, and the hemicellulose degradation proportion was 13.1%. The protein content improved by 29.6%. The lysine content increased by 126%, reaching 11.3 g·kg-1. The threonine content increased from 6.10 to 10.3 g·kg-1. The phytate content was decreased to 3.97 g·kg-1. The in vitro digestibility of dry matter and protein increased to 62.8% and 76.1%, respectively. These results indicated the feasibility of improving the feeding value of wet distiller grains and wheat bran by the symbiosis of T. reesei and C. utilis. © 2024 Society of Chemical Industry.

Effect of heat stress on meat quality of growing rabbits divergently selected for body fat content

High ambient temperature represents an increasingly frequent challenge for animal farming, especially for those animal species more susceptible to heat stress (HS), like the rabbit. The present research studied the impact of different ambient temperatures (T: 20 °C—Control vs. 28 °C—High) on the meat quality of two rabbit lines (L: Fat line, Lean line) obtained after 5 generations of divergent selection for total body fat content. After slaughter, the ground meat of 60 carcases (15 rabbits/treatment) was used for physicochemical and sensory quality evaluation. Overall, high T affected hind leg weight (p < 0.001), pHu (p = 0.001), and oxidative status (p = 0.004) during a shelf-life trial. High T increased meat haem-iron (p < 0.001), decreased lipids (p < 0.001), MUFA (p < 0.001), and PUFA classes (p < 0.001), and consequently, increased water content (p < 0.001). Regarding L effect, Fat line was richer in lipids (p < 0.001) and ash (p = 0.008), but less rich in water (p < 0.001) than Lean line. The content of all fatty acid (FA) classes was therefore significantly higher in Fat line meat (p < 0.001). It can be concluded that the two genotypes differed for proximate composition, haem-iron, FA, and amino acid profiles of carcase meat. High T increased meat pHu, water, haem-iron, and reduced polyunsaturated FA (PUFA) amount. At high T the meat of the Fat line showed higher TBARS, whereas Lean line had higher lysine content. Sensory analysis revealed that high T improved tenderness and extinguished onion off-flavour.

Impact of Precision Feeding During Gestation on the Performance of Sows over Three Cycles.

This study evaluated the impact of precision feeding and bump feeding strategies during gestation on the reproductive performance of sows monitored over three cycles. Four treatments were compared: two constant-concentration feeding strategies (0.53% standardized ileal digestible lysine content; SID Lys) with the feed supply remaining constant (flat feeding; FF) or variable (bump feeding; BF) and two precision feeding strategies based on the InraPorc model considering performance by parity (precision feeding per parity; PFP) or the weight of each sow at breeding (precision feeding by individual; PFI). Sows were followed over three gestation and lactation cycles. In the first cycle (n = 502), the birth-to-weaning piglet mortality for PFP (8.7%) and PFI (10.3%) was lower than for BF (13.8%), with FF (11.3%) being intermediate (p = 0.001). No differences were observed in litter performance during the second cycle (n = 340). During the third cycle (n = 274), the stillborn rate was lower for PFP (6.2%) than for BF (9.1%) and FF (10.4%), with PFI (7.0%) being intermediate (p = 0.01). The BF strategy did not significantly improve sow or litter performance during lactation. Meanwhile, precision feeding could reduce nitrogen (10-13%) and total phosphorus intake (6-9%) with PFP and PFI strategies. Also, the results showed that it could even reduce piglet mortality during lactation.

Biochemical composition and transcriptome analysis revealed nutrients consumption and physiological characteristics of Chinese sturgeon larvae during yolk-sac stage

During the yolk stage, the nutrients of larvae consumed are from the yolk sac, which greatly affect the survival and quality of fish. The aim of this study was to investigate the metabolic changes, physiological characteristics and the related molecular mechanism of Chinese sturgeon (Acipenser sinensis) larvae during yolk stage by biochemical analyses in combination with transcriptome analysis of larvae at three developmental stages (vertical, flat and benthic). The results showed that both total amino acid and protein contents showed a trend of first decreasing and then increasing. The lipid content was stable in the early stages, but decreased significantly in the benthic stage (P < 0.05). In terms of amino acid profile, the contents of leucine, isoleucine and lysine were relatively high in essential amino acids (EAAs), and the contents of glutamate, aspartate and serine were relatively high in non-essential amino acid (NEAA). As for fatty acid content, the most abundant fatty acids are C16:0 (palmitic acid), C18:1 (oleic acid), C18:2n-6 (linoleic acid) and C22:6n-3 (docosahexaenoic acid, DHA). The results of transcriptome analysis showed that 4936 genes were up-regulated and 3079 genes were down-regulated from vertical stage to flat stage, and 1703 genes were up-regulated and 837 genes were down-regulated from flat stage to benthic stage, respectively. According to KEGG enrichment analysis on the differentially expressed genes (DEGs), the up-regulated DEGs mGlu3, mGlu5, NMDAR, PepT1, TAT1, EAAT3, CYP51A1 and CYP7A1 were significantly enriched in pathways related to the nervous system, protein digestion and absorption, steroid synthesis, cholesterol metabolism and bile acid biosynthesis in early development of larvae. Down-regulated DEGs Drg1, NHP2, KRE33 and MPP10 were enriched in the ribosome biogenetic pathway. In late development, up-regulated DEGs, cGAS, IRF3, TRAF2, MHC-I, MHC-II and TAR2, were enriched in pathways associated with innate and adaptive immunity. Down-regulated DKK2 and FGFRs, ITGAs were enriched in Wnt signaling pathway and PI3K-AKT pathway, respectively. In summary, this study revealed that the larval fish consumed protein as the main nutrient from the vertical stage to the flat stage, while mainly consumed lipid from the flat stage to the benthic stage, and highlighted the pathways of DEGs involved in the nervous system and immune system, which would provide new ideal to the high quality larvae production in Chinese sturgeon, thus benefit species protection.

Comparison of the effects of taurine and methionine supplementation on the nitrogen metabolism of beef steers elucidated through plasma metabolome profiling

The objectives of the experiment were to compare the effects of rumen-protected taurine (RPT) and rumen-protected methionine (RPM) on the nitrogen (N) metabolism, plasma biochemical parameters, and metabolomics in beef steers and to clarify whether taurine plays similar roles as methionine (Met) in improving the regulation of N metabolism in beef steers. Six Simmental steers aged 12 months (liveweight 325 ± 7 kg) were used as experimental animals. The experimental treatments included a basal diet, the basal diet + 70.0 g/d RPT and the basal diet + 74.2 g/d RPM. The treatments were assigned in a replicated 3 × 3 Latin square design. Each experimental period included 15 d of adaptation and 5 d of sampling. The results showed that supplementing the diet with RPT or RPM did not affect the apparent nutrient digestibility (P > 0.05). Supplementing the diet with RPT or RPM increased the N retention (P < 0.05) and the N utilization efficiency (NUE) (P < 0.05) and decreased the urinary excretion of 3-Methylhistidine (P < 0.05) and the estimated skeletal protein degradation rate (P < 0.05). Supplementing the diet with RPT increased the plasma concentrations of taurine (P < 0.001), cysteine (P = 0.010), valine (P = 0.013) and total non-essential amino acids (NEAA) (P = 0.047) and tended to increase the plasma concentrations of essential amino acids (EAA) + NEAA (P = 0.087), but it did not affect the plasma concentrations of total EAA (P > 0.05). Supplementing the diet with RPM increased the plasma concentrations of methionine (P = 0.033), lysine (P = 0.047), cysteine (P = 0.007), leucine (P = 0.046), isoleucine (P = 0.046), valine (P = 0.034), total EAA (P = 0.028), total NEAA (P = 0.004) and EAA + NEAA (P = 0.004). The plasma metabolomics profiling revealed that supplementing the diet with RPT upregulated the plasma concentrations of taurine (P < 0.001), L-Cysteine (P = 0.004) and some amino acid (AA) analogues (P < 0.05) and RPM upregulated the plasma concentrations of Met (P = 0.021), L-Isoleucine (P = 0.036), L-Tryptophan (P = 0.006) and some AA analogues (P < 0.05). In conclusion, taurine has similar impacts to Met in improving the N retention and the NUE in beef steers. Taurine deficiency is one of the main reasons that the NUE of beef steers is low. Supplementation of the diet with taurine is beneficial to the N utilization in beef steers.

Left Ventricular Remodeling Predictors in Chronic Heart Failure of Ischemic Etiology.

Aim To identify metabolomic and structure and function markers of remote left ventricular (LV) remodeling in patients with chronic heart failure (CHF) of ischemic etiology and LV ejection fraction (EF) &lt;50%.Material and methods This prospective study included 56 patients with 3-4 NYHA functional class CHF of ischemic etiology (mean age, 66±7 years) and 50 patients with ischemic heart disease (IHD) without signs of CHF (69 [64; 73.7] years). Concentration of 19 amino acids, 11 products of kynurenine catabolism of tryptophan, 30 acylcarnitines with different chain lengths were measured in all participants. The metabolites that showed statistical differences between the comparison groups were then used for the analysis. Echocardiography was used to assess LV cavity remodeling at the time of the CHF patient inclusion in the study and after 6 months of follow-up. Predictors of long-term LV cavity remodeling were assessed for this cohort taking into account statistically significant echocardiographic parameters and metabolites.Results Patients with CHF of ischemic etiology, predominantly (81%) had pathological calculated types of LV remodeling (concentric and eccentric hypertrophy, 46 and 35%, respectively). However, this classification had limitations in describing this cohort. In addition, in this group, the concentrations of alanine, proline, asparagine, glycine, arginine, histidine, lysine, valine, indolyl-3-acetic acid, indolyl-3-propionic acid, C16-1-OH, and C16-OH were significantly (p&lt;0.05) lower, and the concentrations of most medium- and long-chain acylcarnitines were higher than in patients with IHD without signs of CHF. The long-term (6 months) reverse remodeling of the LV cavity in CHF of ischemic etiology was influenced by changes in the interventricular septum thickness (hazard ratio, HR, 19.07; 95% confidence interval, CI, 1.76-206.8; p=0.006) and concentrations of anthranilic acid (HR 19.8; 95% CI 1.01-387.8; p=0.019) and asparagine (HR 8.76; 95% CI 1.07-71.4; p=0.031).Conclusion The presence of an interventricular septum thickness of more than 13.5 mm, anthranilic acid concentrations of higher than 0.235 μM/l, or an asparagine concentration of less than 135.2 μM/l in patients with CHF of ischemic etiology after 6 months of follow-up affects their achievement of LV cavity reverse remodeling.

Amino acid profile and potential utilization purposes of albumin from four types of freshwater fish in Makassar, South Sulawesi, Indonesia

Abstract. Nurfaidah, Asikin AN, Kasmiati, Angraeni. 2024. Amino acid profile and potential utilization purposes of albumin from four types of freshwater fish in Makassar, South Sulawesi, Indonesia. Biodiversitas 25: 4199-4207. Albumin, a high-quality protein, is known for its complete amino acid profile, which is essential for numerous biological functions, including immune response and nutritional status maintenance. This study investigates the amino acid composition of albumin in four freshwater fish species commonly found in Indonesia: Cyprinus carpio, Pangasius pangasius, Channa striata, and Trichopodus trichopterus to asses their potential utilization in sustainable food resources and nutritional and health-related products. Albumin extraction was performed using homogenization and incubation techniques, followed by amino acid analysis using High-Performance Liquid Chromatography (HPLC). The results revealed the presence of 18 amino acids, including 9 essential and 9 non-essential amino acids. The most abundant amino acids were glutamic acid, aspartic acid, leucine, and lysine, with lysine being particularly noteworthy for its role in wound healing and protein synthesis. Significant variations in the amino acid composition were observed across the species, influenced by factors such as diet, habitat, and species-specific physiology. Pangasius pangasius exhibited the highest lysine content (12.01%), while C. carpio had the highest glutamic acid content (24.55%) of total amino acid. These findings suggest that the albumin from these freshwater fish species is a valuable source of high-quality protein, with potential applications in developing nutraceuticals, functional foods, and dietary supplements aimed at improving health and nutritional status. Importantly, the study highlights the potential of these fish species as a sustainable source of protein, contributing to food security and supporting the development of affordable, protein-rich products that can enhance public health while reducing the reliance on conventional livestock-based proteins. This study provides new insights into the amino acid composition of fish albumin from Indonesian freshwater species, contributing to the broader understanding of the nutritional value of local fish resources and their role in promoting sustainable food systems.

Variability in Distillers' Co-Product Compositions and Their Nutritional Availability for Pigs: Insights from a Systematic Literature Review.

This study aimed to examine the nutritional diversity of distillers' co-products in pigs. Two distinct databases were used in this study. The first was compiled through a systematic review of previous scientific publications that have evaluated the chemical composition and nutritional availability of these ingredients for pigs. The final database included information collected from 49 studies published between 2003 and 2022. Most of the studies focused on distillers' co-products produced from corn (90%) and assessed dried distillers grains with solubles (DDGS; 92%). A wide variety of nutritional compositions was observed in the selected studies, with coefficients of variation of 8% for gross energy and 13% for crude protein in dried distillers grains (DDGs). In the general database, half of the studies were published from 2003 to 2015 and the other half from 2015 to 2022. Greater variability in the net energy content and standardized ileal digestible lysine concentrations from 2015 to 2022 were the main concerns. The second database included information on high-protein dried distillers grains (HP-DDGs) collected directly from several feed mills and was built using the results of chemical analyses. These results suggest that the nutritional matrix of co-products should be assessed for each processing plant. Nevertheless, a coefficient of variation of up to 6% was found for crude protein and up to 20% for ether extract. Variability in nutritional composition is an important challenge of using distillers' co-products in pig feed.

The Effect of Nighttime LED Lighting on Tomato Growth, Yield, and Nutrient Content of Fruits

Food insecurity is a top economic and national security concern in many countries, and scientists worldwide are working to increase crop productivity in order to address this issue. In line with this information, the present study aimed to test the possibility of improving the yield and fruit quality of two tomato cultivars, namely ‘Vspyshka’ and ‘Lyana’. The effect of LSL (light of sodium lamps—control) and the short additional 4 h treatment of nighttime LED lighting (LSL+night LED) with an increase in the proportion of red, blue, and far-red spectra on tomato fruit yield as well as its physiological, biochemical, and consumer attributes were compared in this study. The results suggested that LSL+night LED significantly increased soluble solids concentration, vitamin C content, and polyphenolic compounds of tomato fruits, taking into account the varietal characteristics. Moreover, a moderate to high relationship was also observed between the polyphenolic complex, vitamin C content, and antioxidant activity. It was concluded that the LSL+night LED could further enhance the relationship between polyphenols and antioxidants, as well as soluble solids concentration. LSL+night LED treatment also provided an increased accumulation of five essential amino acids associated with the taste characteristics of fruits, namely histidine, valine, threonine, licin, and the sum of isoleucine. In addition, the contents of lysine and methionine increased in the ‘Lyana’ cultivar. LSL+night LED treatment was also noted to have a less pronounced effect on the contents of aspartic acid and asparagine, as bio stimulators of plant growth processes, as well as the amino acids arginine, serine, glycine, and tyrosine, which were additionally consumed to restore photosynthesis. LSL+night LED treatment reduced the concentration of nitrates in fruits, which is a toxic element for human health. Overall, the results of the study are believed to be demanded in practical applications, with potential benefits in improving the elements of resource-saving technology for growing vegetable crops.

The Influence of Sowing Rate and Foliar Fertilization on the Yield of Some Triticale Varieties in the Context of Climate Change in Northwest Romania

Triticale is recognized worldwide because of its high protein and lysine contents, high production capacity, and adaptability to biotic and abiotic stress conditions, these qualities being taken over from wheat and rye. Triticale is widely used in various fields, such as animal feed in various forms, medicine, baking, beer and alcohol brewing, cellulose, bioethanol industry, and many others. Thus, the demand for triticale grain is increasing, and this has led to the research and improvement in culture technology to obtain superior products, both quantitatively and qualitatively. The purpose of this study was to identify the best varieties of triticale cultivated in the northwest of Romania, sown in different plots, and fertilized on the ground. Additionally, this study was carried out over a period of two years at the Livada Agricultural Research and Development Station, Satu Mare County. This study was located on acidic soil with a pH between 5.19 and 6.65 and a humus content of 2.82%. The climatic conditions in the reference period were extremely variable; in the first year, a deficit of more than 90 mm of precipitation was registered, and in the second year of this study, an increase of more than 34 mm. The effects of additional fertilization were influenced by the level of precipitation. In 2023, additional fertilization with foliar fertilizer brought production increases of 884 kg/ha, compared with 2022, where foliar fertilization in drought conditions led to a decrease in production. The Utrifun variety proved to be the most productive; this foliar fertilized and with biostimulator, sown at 550 seeds/m2, recorded an increase in production of over 4500 kg/ha compared with the Negoiu control and sown at 650 seeds/m2 and fertilized with foliar fertilizer had an increase of over 4370 kg/ha compared with the Negoiu control. It recorded a production of 9700 kg/ha sown at 550 seeds/m2 and fertilized only on the soil, and sown at 450 seeds/m2 and additionally fertilized with foliar fertilizer recorded a production of 10.900 kg/ha. Utrifun was followed by Zvelt which, sown at 450 seed/m2 and fertilized on the soil, recorded 9500 kg/ha, ensuring an increase of 1800 kg/ha compared with the Negoiu control. The lowest production was achieved by the Tulnic variety, which is 2022, sown at 650 seed/m2 and fertilized on the ground, recorded 5874 kg/ha, 1621 kg/ha less than the control variant. The increases in production obtained by these varieties will be confirmed in a subsequent study under different climatic conditions.

Outcomes of kidney transplantation in patients with lysinuric protein intolerance.

Lysinuric protein intolerance (LPI) is a metabolic disorder that leads to dysfunctional intestinal absorption and kidney clearance of cationic amino acids. Chronic kidney disease develops in many LPI patients and leads to end-stage kidney disease in at least 10% of patients. Since data on kidney transplants in LPI patients are limited, we analysed the outcomes of LPI patients after transplantation in Finland. This retrospective cohort study includes all Finnish LPI patients who have received a kidney transplant. The data were collected from the Finnish Transplant Registry and electronic medical records from 2005 through May 2023 or patient death. The plasma amino acid profile was analysed before and after transplantation. Eight LPI patients (75% female, mean age at transplant 41.9years) received a kidney allograft and two of the patients received a second transplant. Nine transplants were from deceased donors and one was from a living donor. Acute rejection occurred after four transplantations (two T-cell mediated and two antibody mediated). One patient died 6months after transplantation due to alveolar proteinosis. Apart from lower citrulline and higher lysine concentrations, plasma amino acid levels showed no changes after transplantation. The 1-, 5- and 10-year graft survivals were 80%, 68.6% and 51.4%, and patient survivals were 88%, 86% and 50%, respectively. Kidney transplantation is feasible in patients with LPI, although the acute rejection rate seems high and severe complications such as pulmonary alveolar proteinosis may occur. Transplantation led to changes in plasma citrulline and lysine concentrations.

Changes in amino acid concentrations and the gut microbiota composition are implicated in the mucosal healing of ulcerative colitis and can be used as noninvasive diagnostic biomarkers

BackgroundMucosal healing is the therapeutic target for ulcerative colitis (UC). While amino acids (AAs) and the gut microbiota are known to be involved in the pathogenesis of UC, their specific roles in mucosal healing have not been fully defined.ObjectivesTo longitudinally assess the changes in AA concentrations and the gut microbiota composition in the context of mucosal healing in UC patients, with the aim of identifying new biomarkers with predictive value for mucosal healing in UC patients and providing a new theoretical basis for dietary therapy.MethodsA total of 15 UC patients with infliximab-induced mucosal healing were enrolled. Serum and fecal AA concentrations before and after mucosal healing were determined via targeted metabolomics. A receiver operating characteristic (ROC) curve was plotted to evaluate the value of different AAs in predicting mucosal healing in UC patients. The changes in the composition of the fecal gut microbiota were analyzed via metagenomics, and bioinformatics was used to analyze the functional genes and metabolic pathways associated with different bacterial species. Spearman correlation analyses of fecal AAs with significantly different concentrations and the differentially abundant bacterial species before and after mucosal healing were performed.Results1. The fecal concentrations of alanine, aspartic acid, glutamic acid, glutamine, glycine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine were significantly decreased after mucosal healing. The serum concentrations of alanine, cysteine and valine significantly increased, whereas that of aspartic acid significantly decreased. Glutamic acid, leucine, lysine, methionine and threonine could accurately predict mucosal healing in UC patients, and the area under the curve (AUC) was > 0.9. After combining the 5 amino acids, the AUC reached 0.96. 2. There were significant differences in the gut microbiota composition before and after mucosal healing in UC, characterized by an increase in the abundance of beneficial microbiota (Faecalibacterium prausnitzii and Bacteroides fragilis) and a decrease in the abundance of harmful microbiota (Enterococcus faecalis). LEfSe analysis identified 57 species that could predict mucosal healing, and the AUC was 0.7846. 3. Amino acid metabolic pathways were enriched in samples after mucosal healing, was associated with the abundance of multiple species, such as Faecalibacterium prausnitzi, Bacteroides fragilis, Bacteroides vulgatus and Alistipes putredinis. 4. The fecal concentrations of several AAs were negatively correlated with the abundance of a variety of beneficial strains, such as Bacteroides fragilis, uncultured Clostridium sp., Firmicutes bacterium CAG:103, Adlercreutzia equolifaciens, Coprococcus comes and positively correlated with the abundance of several harmful strains, such as Citrobacter freundii, Enterococcus faecalis, Klebsiella aerogenes, Salmonella enterica.ConclusionAltered concentrations of amino acids and their associations with the gut microbiota are implicated in the mucosal healing of UC patients and can serve as noninvasive diagnostic biomarkers.