Luteus Bacteria Research Articles

Макроміцети Національного природного парку «Гуцульщина» та їх антибактеріальні властивості

The ability of ethanol extracts of 20 species of macromycetes to inhibit the growth of bacterial cultures was studied. Their influence on indicators of superoxide dismutase and catalase activities in relation to three types of test cultures of microorganisms was established. Extracts of Cantharellus cibarius, Grifola frondosa Laetiporus sulphureus, Leccinum scabrum, Mycena leptocephala, Russula rosea, Strobilomyces strobilaceus showed the largest diameters of growth retardation of microorganisms of test cultures. The ability to counteract bacteria is a species-specific feature and does not depend on the trophic timing of macromycetes, the same applies to the ability to suppress the catalase activity of microorganisms. Extracts of macromycetes, which provoked inhibition of the growth of cultures, led to changes in the SOD activity of the studied bacteria: during incubation of M. luteus culture with mushroom extracts, which showed an inhibitory effect on the growth of microorganisms with a diameter of 8-12 mm, superoxide dismutase activity increased 2-3 times, during incubation of M. luteus bacteria with extracts that inhibited growth with a diameter greater than 12 mm, the enzymatic activity increased 8-10 times. The SOD activity of B. spizizeni increased by 40-80% at the 24th hour of the experiment with extracts, the use of which is accompanied by the formation of a 10 mm zone of growth inhibition, but already after 48 hours of the experiment, the indicators of the SOD activity of B. spizizeni decreased to the control values. SOD activity of E. coli increased on the 24th hour of the experiment by 1.4-1.6 times relative to the control values, even with the use of extracts that provoked inhibition of the growth of cultures with a diameter of 8 mm and remained high on the 48th hour of the experiment (in 1.2-1.3 times).

Anti-bacterial Effect and Characteristics of Gold Nanoparticles (AuNps) Formed with Vitex negundoPlant Extract.

Our current study reports the anti-bacterial activity of the gold nanoparticles (AuNps) synthesized by the green synthesis method using Vitex negundo plant leaves. The aqueous extract of Vitex negundo plant leaves are acting as the capping and stabilizing agent in the synthesis of AuNps. It is already evident from earlier studies that Vitex negundo is an abundant source of polyphenols, flavonoids, terpenoids, and many other biologically active compounds. The present study reveals the potential of biologically active compounds from the plant in the reduction reaction of chloroauric acid (HAuCl4) into gold nanoparticles. The green synthesis method is adapted instead of the chemical method, which is toxic and more expensive. The gold nanoparticles subjected to characterization with the help of UV-visible spectroscopy, FTIR to determine functional groups, light scattering to estimate size and uniformity, scanning emission microscopy with EDX for accurate size and shape of AuNps, and X-ray diffraction to reveal the crystalline structure. The characteristics of AuNps formed are UV reading at 520nm, FTIR showing the presence of phenols and alkenes, DLS, SEM, and XRD confirming the spherical shape with the size around 70-90nm. The anti-bacterial activity of the gold nanoparticles is evaluated against four different species of bacteria, each two gram-positive and gram-negative. The gold nanoparticles formed by Vitex negundo show good anti-bacterial activity against Salmonella typhi and M. luteus bacteria with a zone of inhibition of 6mm and 2mm respectively. Furthermore, the cytotoxic activities of the gold nanoparticles are yet to be known to their full extent.

Antimicrobic Activity of IPPU Padang (Ammannia octandra L.f.) Leaves Ethanol Extract against Skin Pathogenic Microbials

Ippu Padang plant (Ammannia octandra L.f.) belongs to the family of Lythraceae, a hardy plant that can grow to a height of 50 cm. According to previous research, Ippu Padang leaves contain glycosides, alkaloids, flavonoids and tannins. The presence of alkaloid compounds, flavonoids and tannins is predicted to have potential as an antifungal agent against fungi and antibacterial agent. This research used an experimental method. The steps included collecting plant material, plant identification, processing plants into Simplicia powder, phytochemical screening of Simplicia powder, extracting Simplicia by maceration method using ethanol solvent, antifungal and antibacterial activity test using agar diffusion method and determination of inhibition diameter of leaf ethanol extract. The results of phytochemical screening showed that ippu Padang leaves contained secondary metabolites, namely alkaloids, flavonoids, glycosides, anthraquinone glycosides, tannins, saponins and steroids. The results also revealed that the concentration with the largest inhibitory diameter was 400 mg/ml, namely 12.4 mm against the Candida albicans, 17.46 mm against the Dermacoccus nishinomiyaensis bacteria, 18.53 mm against the Micrococcus luteus bacteria, 19.38 mm against the Pseudomonas aeruginosa bacteria, and 17.71 mm against Staphylococcus epidermidis bacteria. It was concluded that the ethanol extract of ippu Padang leaves could inhibit the growth of the Candida albicans, the bacteria Dermacoccus nishinomiyaensis, Micrococcus luteus, Pseudomonas aeruginosa and Staphylococcus epidermidis.

Synthesis and Antimicrobial Activity of Some of Isoindolin-1-One-3-Phosphonates under Mild and Solvent-Free Conditions

A range of N-substituted isoindolin-1-one-3-phosphonate compounds 4 under mild and solvent-free conditions was prepared. The synthesized compounds were screened for their antimicrobial activities against gram-positive bacterial strains (Micrococcus luteus, Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus), a gram-negative bacterial strain (Salmonella typhimurium). The compounds 4a and 4 b were the most active against M. luteus bacteria with inhibition zones (IZ) of 35 and 24 mm, respectively, and 4a was the most active against L. monocytogenes ATCC 1911 with an IZ of 22 mm. Minimum inhibitory concentration (MIC) of the synthesized compounds 4 and the two standards tetracycline and fluconazole was assessed against the four indicator microorganisms: L. monocytogenes ATCC 1911, S. aureus ATCC 6538, S. typhimurium ATCC 14028 and C. albicans (ATCC 90028). The lowest MIC values were obtained for the compound 4d. Against C. albicans (ATCC 90028).

Kosmotropic and chaotropic effect of biocompatible Fe3O4 nanoparticles on egg white lysozyme; the key role of nanoparticle-protein corona formation

Accurate understanding of the interaction between different types of nanoparticles and proteins is very important in various fields, especially in Nano-biotechnology. Concentration-dependent nanoparticles can cause structural changes in proteins. There are several procedures to increase the efficiency of nanoparticles in these interactions. In this study, the effect of magnetic nanoparticles (MNPs) including Fe3O4 and Fe3O4@RA (Rosmarinic acid coated with Fe3O4) was estimated on the stability and activity of egg white Lysozyme (EWL). The synthesis of MNPs was done using co-precipitation method; then they were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), vibrating sample magnetometer (VSM), CHNS elemental analysis, dynamic light scattering (DLS) and field emission scanning electron microscopy (FE-SEM). The results of fluorescence analysis showed that both MNPs can have two opposite effects on the tertiary structure of EWL, depending on their concentration. Accordingly, at the concentrations below a concentration threshold (0.0083 and 0.0067 for Fe3O4 NPs and Fe3O4@RA, respectively), they improve the structure of the EWL (kosmotropic-like effect) and at the concentrations above it, they gradually cause its degradation (chaotropic-like effect). Changes in the protein activity against Micrococcus luteus bacteria based on UV analysis data were also fully consistent with the fluorescence analysis. In order to study the secondary structure of EWL, CD Spectra showed an increase in the helicity of EWL after interaction with both MNPs (3.7% and 1.1% increase in the helicity for 0.02 and 0.1 mg.mL−1 of Fe3O4 respectively, and also 5.5% and 2% for 0.02 and 0.1 mg.mL−1 of Fe3O4@RA respectively) and a decrease in this factor after interaction with pure RA (0.9% and 4.1% decrease in the helicity for 0.02 and 0.1 mg.mL−1 of pure RA respectively).

Impact of the Alkyl Side Chains of Cations and Anions on the Activity and Renaturation of Lysozyme: A Systematic Study Performed Using Six Amino‐Acid‐Based Ionic Liquids

AbstractThe impact of the structure of ionic liquid on the activity and renaturation of lysozyme from the hen egg white (HEWL) was investigated. A set of six ionic liquids based on morpholinium cation and N‐acylated amino acids as anions were exploited. The matrix used was based on two homologous series: one with N‐acetyl‐L‐valinate [Val] as a common anion and morpholinium cation with a different number of carbon atoms in the n‐alkyl chain (n=2, 4, 6, 8); and second, a common N‐butyl‐N‐methylmorpholinium cation and different N‐acylated amino acids. Interactions of ionic liquids with HEWL were explored by analyzing the changes in the activity of the enzyme based on the rate of hydrolysis of a β‐1,4‐glycosidic bond between components of peptidoglycan in the cell wall of Micrococcus luteus bacteria. The results demonstrated various mechanisms of interaction of cations and anions with HEWL, and hence, different trends in the association between the number of carbon atoms and the given property.

1,2,3-Triazoles based 3-substituted 2-thioquinoxalines: Synthesis, anti-bacterial activities, and molecular docking studies

Abstract New 1,2,3-triazoles based on 3-Substituted 2-thioquinoxalines were synthesized via a copper-catalyzed click reaction. The new 1,2,3-triazoles were screened for their in vitro antibacterial activities and were subjected to molecular docking studies. The starting materials included, 3-substituted-2-propargylthioquinoxalines were prepared from 2,3-dichloroquinoxaline, aliphatic amines, sodium sulfide, and propargyl bromide. The click of 2-propargythioquinoxalines with aryl azides catalyzed by copper(II) salen complex afforded novel 1,2,3-triazole-linked thioquinoxalines derivatives. The use of salen copper(II) complex as a catalyst, increased the reaction rate and reduced the loading of the toxic copper species. Results obtained from the in vitro anti-bacterial activities of the synthesized compounds revealed that compounds 7e, 7g, and 7k are active against Bacillus subtilis and Micrococcus luteus bacteria. Furthermore, in silico molecular docking results stipulated a sign of good correlation between experimental activity and calculated binding affinity. Docking studies proved 7e as the most potent compound. In addition, the binding maps exhibited activities that may attribute to the existence of electron donating and lipophilic group at para position of phenyl ring and hydrophobic interactions and hydrogen bond with the protein active sites.

Antibacterial activity of [1Fe-2S]- and [2Fe-2S]-nitrosyl complexes as nitric oxide donors

The biological activity of a series of sulfur-nitrosyl iron complexes (NICs) depends on the structure of the ligands and the position of the functional groups in the aromatic ring of the ligand. Differences in susceptibility of the gram-positive and gram-negative bacteria to the NICs was found. The series of the studied NICs suppresses the formation of biofilms of Micrococcus luteus bacteria. The obtained results demonstrate that the studied complexes can serve as the basis for the preparation of antibacterial agents.

Surface functionalization of electrospun PAN nanofibers with ZnO–Ag heterostructure nanoparticles: synthesis and antibacterial study

In this work, we have prepared polyacrylonitrile (PAN) polymer nanofibers by electrospinning method. The surface of the electrospun PAN nanofibers membrane has been functionalized with ZnO–Ag heterostructure nanoparticles by using three different chemical pathways such as reflux, blending, hydrothermal methods and accordingly the prepared composite nanofibers membranes were named as PAN/ZnO–Ag (R), PAN/ZnO–Ag (B) and PAN/ZnO–Ag (H) respectively. The obtained heterostructure nanoparticles functionalized PAN nanofibers membranes were characterized using ATR-FTIR, XRD, FESEM and TEM analytical techniques. From the FESEM and TEM images it was clearly observed that 20–30 nm size spherical nanoparticles have been decorated uniformly on the surface of PAN nanofibers. XRD study confirmed the formation of ZnO–Ag mixed/hybrid nanoparticles on PAN nanofibers surface. The ZnO–Ag heterostructure nanoparticles functionalized PAN nanofibers membranes were used for antibacterial application. It was observed from inhibition zone study that the ZnO–Ag heterostructure nanoparticles functionalized PAN nanofibers membrane shows excellent antibacterial properties towards both gram-negative Escherichia coli and gram-positive Micrococcus luteus bacteria than their single component counterparts. Thus this study demonstrated the simple and cost-effective approach to develop antibacterial functional membrane that has many potential applications in water and air filtration, protective mask, textile and packaging industries.

Antibacterial active open-porous hydroxyapatite/lysozyme scaffolds suitable as bone graft and depot for localised drug delivery.

An engineered synthetic scaffold for bone regeneration should provide temporary structural support and a medium for controlled and localised release of bioavailable medical drugs. In this work, a method is proposed to incorporate biologically active agents without impairing agent activity into open-porous resorbable hydroxyapatite scaffolds. Scaffolds are obtained by a one-pot freeze gelation process and loaded with different amounts of lysozyme, a model macromolecular drug with antibacterial activity. The antibacterial activity is tested by submerging hydroxyapatite scaffolds with 0.5 to 2.5 wt.% lysozyme into two different bacteria stock solutions. A complete dieback of M. luteus bacteria when in contact with the scaffolds is observed. Higher lysozyme amount in the scaffold leads to faster dieback. In contact with scaffolds containing 2.5 wt.% lysozyme after 30 min, no viable bacteria can be observed. An amount of 0.5 wt.% lysozyme in the scaffolds is sufficient to kill all bacteria after a contact time of 24 h. For L. innocua, a bacteriostatic effect is observed. The scaffolds have spongiosa-like stability and are suitable bone implant substitutes. As agents are released from the scaffolds by degrees over a time period of at least 9 days, they are particularly attractive as depot for localised drug delivery of bioactive macromolecular drugs.

Laser Treatment of Cp-Ti & Ti-Ni Alloy Coated with Hydroxyapatite/Silver Nanoparticles for Biomedical Applications

In this investigation, the biocompatibility of alloying elements for Cp-Ti, and Ti-Ni titanium alloys was tested in order to estimate their suitability for biomaterial components. As this alloys used as orthopedic and dental implants. Cp-Ti was tested as reference material. The investigation included the mechanical properties as corrosion and microhardness. The susceptibility of bacteria to the Ag-HA surface was also evaluated by assessing the inhibition of luminescence of Escherichia coli, pseudomonas aeruginosa, bacillus cereus, and micrococcus luteus bacteria.

Synthesis, antimicrobial and anti-inflammatory activities of some novel 5-substituted imidazolone analogs

Abstract In view of potent antimicrobial and anti-inflammatory activities exhibited by 5-substituted imidazolones, a variety of novel imidazolone analogs 3a-l were synthesized by the condensation of different substituted oxazolones 1 with various aromatic amines 2. All the synthesized compounds were screened for in vitro activities against a panel of Gram-positive and Gram-negative bacteria and the yeast-like pathogenic fungus Candida albicans. Several analogs produced good or moderate activities particularly against the tested Gram-positive bacteria Micrococcus luteus and Gram-negative bacteria Pseudomonas aeruginosa and. Meanwhile, compounds 3b and 3c displayed marked antifungal activity against C. albicans. In addition, the in vivo anti-inflammatory activity of the synthesized compounds was determined using the carrageenin-induced paw oedema method in rats. Two of 5-substituted imidazolone derivatives, 3k and 3d show good anti-inflammatory activity. The structures of all the newly synthesized compounds were elucidated using IR, 1H NMR and 13C NMR.

Anti-microbial, cytotoxicity, and necrotic ripostes of Pimpinella anisum essential oil

Pimpinella anisum essential oil (EO) residuals and its biotic possessions were deliberated. EO was identified using UPLC MS/MS analysis. Inhibitory effect of oil and its preparations were studied using disc-diffusion and Minimum Inhibitory Concentration (MIC) tests, and using corn oil and Streptomycin as controls. Tolerant ability of EO treated Hep G2 cells was appraised using MMT assess, and 0.4, 0.8, 1.2 and 1.6% (v/v) of EO for 3h were used for flow cytometric exploration of cell cycle regression. UPLC MS/MS inquiry revealed that EO major ingredients were phenyl propanoids followed by monoterpenes (hydrocarbons and phenolics), and trans-anethole followed by cis-anethole, estragole, linalool, α-terpineol, and methyl eugenol were the major EO constituents. EO has high inhibitory effect for Salmonella typhi, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Micrococcus luteus. Corn oil inhibited E. coli and M. luteus at high concentrations. Streptomycin gave higher inhibition with all bacteria. However both have similar effects with E. faecalis, S. typhi, and M. luteus bacteria. Spraying potato tubers with EO emulsified with soap prior to Ralstonia solanacearum infection and preservation at 4°C were kept healthy without any rot symptoms. Treating Hep G2 cells with EO for 24h caused a substantial cytotoxicity as a concentration reliant, and exhibited a significant cytotoxicity at the higher concentrations. Cell cycle examination of PI-stained did not indicate any changes in the cell cycle regression. There is no substantial escalation in the extension of apoptotic/necrotic Hep G2 cells in sub-G1 segment.

Wound Healing Bionanocomposites Based on Castor Oil Polymeric Films Reinforced with Chitosan-Modified ZnO Nanoparticles.

Castor oil (CO), which is a readily available, relatively inexpensive, and environmentally benign nonedible oil, has been successfully used as matrix material to prepare biocompatible and biodegradable nanocomposite films filled with chitosan (CS)-modified ZnO nanoparticles. The biocomposites were synthesized via a simple and versatile solution mixing and casting method. The morphology, structure, thermal stability, water absorption, biodegradability, cytocompatibility, barrier, mechanical, viscoelastic, antibacterial, and wound healing properties of the films have been analyzed. FT-IR spectra were used to obtain information about the nanoparticle-matrix interactions. The thermal stability, hydrophilicity, degree of porosity, water absorption, water vapor transmission rate (WVTR), oxygen permeability (Dk), and biodegradability of the films increased with the CS-ZnO loading. The WVTR and Dk data obtained are within the range of values reported for commercial wound dressings. Tensile tests demonstrated that the nanocomposites displayed a good balance between elasticity, strength, and flexibility under both dry and simulated body fluid (SBF) environments. The flexibility increased in a moist atmosphere due to the plasticization effect of absorbed water. The nanocomposites also exhibited significantly enhanced dynamic mechanical performance (storage modulus and glass transition temperature) than neat CO under different humidity conditions. The antibacterial activity of the films against Escherichia coli, Staphylococcus aureus, and Micrococcus luteus bacteria was investigated in the presence and the absence of UV light. The biocide effect increased progressively with the CS-ZnO content and was systematically stronger against Gram-positive cells. Composites with nanoparticle loading ≤5.0 wt % exhibited very good in vitro cytocompatibility and enabled a faster wound healing than neat CO and control gauze, hence showing great potential to be applied as antibacterial wound dressings.

Electrospun scaffolds of polylactide with a different enantiomeric content and loaded with anti-inflammatory and antibacterial drugs

Polylactide (PLA) electrospun microfibers were prepared and loaded with triclosan (TCS), ketoprofen (KTP), or their combination to obtain multifunctional scaffolds with bactericide and anti-inflammatory properties. Continuous and porous fibers with diameters in the micrometer scale and a unimodal distribution were successfully attained using a dual-electrospinning technique. Dual drug-loaded scaffolds showed a peculiar release that was in contrast to the single drug-loaded systems, which suggested the establishment of intermolecular interactions that delayed TCS and KTP release. Antimicrobial activity of all TCS-loaded electrospun scaffolds was demonstrated against E. coli and M. luteus bacteria; and furthermore, KTP-loaded samples slightly showed bactericide activity. Biocompatibility of scaffolds was evaluated by adhesion and proliferation assays, and interestingly, the dual drug-load systems were able to support high TCS doses without adverse effects.

Antibacterial activity of northern Ontario medicinal plant extracts

Hassan, H. M., Jiang, Z.-H., Asmussen, C., McDonald, E. and Qin, W. 2014. Antibacterial activity of northern Ontario medicinal plant extracts. Can. J. Plant Sci. 94: 417–424. In the present study, the antibacterial activity (in vitro) of the leaf and/or flower crude extracts of Anaphalis margaritacea (L.) Benth & Hook.f., Grindelia squarrosa (Pursh) Dunal, Apocynum androsaemifolium L., Arctostaphylos uva-ursi (L.) Spreng, Cornus canadensis L. and Xanthium strumarium L. medicinal plants was analyzed through the hole-plate diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays against Escherichia coli, Aeromonas caviae, Paenibacillus alvei, Micrococcus luteus, Mycobacterium avium subsp. avium and Bacillus cereus bacteria. In addition, the time-kill dynamic processes of these extracts against A. caviae, B. cereus, P. alvei, and M. luteus bacteria were also tested. The leaf and flower extracts of Anap. margaritacea and G. squarrosa possess significant antibacterial activity against all the bacteria tested, with inhibition of A. caviae, P. alvei and M. luteus within 1–12 h of incubation at MBC. Particularly, at higher concentrations of the Anap. margaritacea flower crude extract (2–3×MBC), inhibition of A. caviae, B. cereus, P. alvei, and M. luteus bacteria is achieved between 0.5 and 4 h of incubation. In addition, these extracts exhibit high inhibition diameters (majority > 18 mm) and low MIC and/or MBCs (majority=1.25 mg mL−1). In contrast, the leaf extracts of Arct. uva-ursi, X. strumarium, Apoc. androsaemifolium and C. canadensis plants demonstrate moderate to low activity. These results indicate that extracts from Anap. margaritacea and G. squarrosa could be a source of novel antimicrobial scaffolds, compounds or pharmacophores with implications in the pharmaceutical industry and medicine.

Capillary electrophoresis of microbial aggregates

Uncontrolled aggregation of bacterial cells is a significant disadvantage of electrophoretic separations. Various aspects of the electrophoretic behavior of different strains of Gram-positive Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus(1), and Micrococcus luteus bacteria and Gram-negative Escherichia coli bacteria were investigated in this study. Our findings indicate that bacteria can be rapidly analyzed by CZE with surface charge modification by calcium ions (Ca(2+)). Bound Ca(2+) ions increase zeta potential to more than 2.0 mV and significantly reduce repulsive forces. Under the above conditions, bacterial cells create compact aggregates, and fewer high-intensity signals are observed in electropherograms. The above can be attributed to the bridging effect of Ca(2+) between bacterial cells. CE was performed to analyze bacterial aggregates in an isotachophoretic mode. A single peak was observed in the electropherogram.

Sulfur-containing components of supercritical garlic extracts and their synthetic analogs as potential biocides

We have shown that a specific component of supercritical (SC) garlic extract and some synthetic disulfide analogs are active as antimicrobials. In the course of this study, six compounds (diallyl disulfide, 1-propenylallyl disulfide, methylallyl disulfide, diallyl trisulfide, 2-vinyl-4H-1,3-dithiin, and 3-vinyl-4H-1,2-dithiin) were isolated from SC garlic extract and identified. Also, two novel sulfoxide-containing garlic analogs were synthesized. All isolated and synthetic substances were tested as potential antimicrobials against gram-positive bacteria Bacillus cereus, Mycobacterium smegmatis, and Micrococcus luteus and gram-negative bacteria Pseudomonas aurantiaca. Diallyl disulfide and diallyl trisulfide exhibited the best activity against both gram-positive and gram-negative bacteria.

Bacteriolytic enzymes from the blood plasma of the sheep

In the present work the studies ofbacteriolytic factors from sheep blood plasma have been performed. Three novel enzymes have been identified and characterized. Two of them have a molecular weight 15 +/- 2 kDa and able to lyse the gram-negative Escherichia coli bacteria. The third enzyme has a molecular weight 34 +/- 4 kDa and is able to lyse both gram-negative Escherichia coli and gram-positive Micrococcus luteus bacteria. The bacteriolytic reactions have been studied for all three enzymes; particularly, pH-optima have been identified with respect to the substrate. To identify the enzymes trypsinolysis and consequent MALDI-TOF mass spectrometry studies were performed. The results were compared to data from publicly available databases, such as Swiss-Prot, NCBI, MSDB.

In vitro Antibacterial Potential Activity of the Methanolic and Aqueous Extracts of Corrigiola telephiifolia Pourr. and Mesembryanthemum nodiflorum

The present study was conducted to investigate antibacterial activity of methanolic and aqueous extracts obtained from aerial parts of Mesembryanthemum nodiflorum and Corrigiola telephiifolia Pourr., root, using the disc-diffusion and microdilution assays. The extracts were tested against Gram-positive bacteria: Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus and Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The methanol extracts of plants screened revealed promising antibacterial activities against most pathogens bacteria. Maximum activity of corrigiola telephiifolia Pourr., and Mesembryanthemum nodiflorum methanol extracts were observed against Pseudomonas aeriginosa, Klebsiella pneumoniae, Escherichia coli and Micrococcus luteus with minimal inhibitory concentration (MIC) values ranging from 3.12 to 6.25 mg/ml. The antibacterial activity of aqueous extract was less than this of methanolic extract (MIC 3.12 and 12.5 mg/ml) were observed against all tested bacteria. These species might provide promising therapeutic agents against infections with multi-resistant and pathogens bacteria.