Anti-microbial, cytotoxicity, and necrotic ripostes of Pimpinella anisum essential oil

Abstract

Pimpinella anisum essential oil (EO) residuals and its biotic possessions were deliberated. EO was identified using UPLC MS/MS analysis. Inhibitory effect of oil and its preparations were studied using disc-diffusion and Minimum Inhibitory Concentration (MIC) tests, and using corn oil and Streptomycin as controls. Tolerant ability of EO treated Hep G2 cells was appraised using MMT assess, and 0.4, 0.8, 1.2 and 1.6% (v/v) of EO for 3h were used for flow cytometric exploration of cell cycle regression. UPLC MS/MS inquiry revealed that EO major ingredients were phenyl propanoids followed by monoterpenes (hydrocarbons and phenolics), and trans-anethole followed by cis-anethole, estragole, linalool, α-terpineol, and methyl eugenol were the major EO constituents. EO has high inhibitory effect for Salmonella typhi, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Micrococcus luteus. Corn oil inhibited E. coli and M. luteus at high concentrations. Streptomycin gave higher inhibition with all bacteria. However both have similar effects with E. faecalis, S. typhi, and M. luteus bacteria. Spraying potato tubers with EO emulsified with soap prior to Ralstonia solanacearum infection and preservation at 4°C were kept healthy without any rot symptoms. Treating Hep G2 cells with EO for 24h caused a substantial cytotoxicity as a concentration reliant, and exhibited a significant cytotoxicity at the higher concentrations. Cell cycle examination of PI-stained did not indicate any changes in the cell cycle regression. There is no substantial escalation in the extension of apoptotic/necrotic Hep G2 cells in sub-G1 segment.