Lumpy Skin Disease Research Articles

First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq.

Lumpy skin disease (LSD) is one of the most important infectious bovine diseases in Iraq in the last 10 years; however, the current study represents the first investigation to confirm the disease in buffaloes as well as ticks with estimation the association of positivity to clinical vital signs and risk factors. A total of 150 buffaloes were subjected for blood sampling, skin lesions and ticks. All the collected samples; 150 blood, 13 skin lesions, and 29 tick samples, were examined molecularly using the conventional and real-time PCR assays. The total positive results of blood, skin and ticks by conventional PCR were 5.33, 7.69 and 0%, respectively; while for real-time PCR, it was 15.33, 7.69 and, 0%, respectively. Insignificant differences were showed between values of temperature, pulse and respiratory rates of LSD positive and negative buffaloes by the conventional and real-time PCR assays. The association of positive conventional PCR results to risk factors (age, sex and region) was revealed a significant increase in prevalence and risk of LSD in buffaloes aged < 1 year; but for gender, insignificant variation in prevalence but not risk was seen between females and males. In case of different geographical region, significant higher prevalence was reported in Wasit; while, buffaloes of Maysan and Wasit were appeared at higher risk than those of Dhi-Qar. Regarding real-time PCR, insignificant differences were found between values of < 1, 1-4 and > 4-8 years age old, but not in group of >8 that showed a significant decline in positivity (0%). For sex, insignificant variation in prevalence, but not risk, was seen between females and males. Concerning region, buffaloes of Wasit province were recorded a significant higher values of prevalence and risk than other regions. LSD in buffaloes is mainly sub-acute, and PCR appeared to be a suitable diagnostic method in detection of infection; however, furthermore studies are necessary.

The first study on the impact of lumpy skin disease outbreaks on monthly milk production on dairy farms in Khon Kaen, Thailand.

Outbreaks of lumpy skin disease (LSD) have resulted in substantial economic losses to the dairy industry in Thailand. This study aimed to determine the influence of LSD outbreaks on monthly milk production levels. Milk production for dairy farms located in Khon Kaen Province, Thailand, belonging to the Khon Kaen Dairy Cooperative, was affected by LSD outbreaks from May to August of 2021. The resulting data were analyzed using general linear mixed models. It was estimated that the LSD outbreak caused economic losses totaling 2,413,000 Thai Baht (68,943 USD) over the outbreak period. The monthly farm milk production level in May differed from the levels in June and August. Dairy farmers experienced losses between 8.23 and 9.96 tons of milk each month, which equated to between 4180 and 14,440 Thai Baht (119.43 and 412.57 USD) in monthly income. This study demonstrated that LSD outbreaks on dairy farms resulted in significant farm milk production losses. Our findings will increase awareness among authorities and stakeholders in the dairy industry of Thailand, as well as to assist in the prevention of future LSD outbreaks and minimize the negative impacts of LSD.

Phylogenetic and sequence analysis of Lumpy skin disease virus circulating in some governorates of Egypt during 2019/2020.

Phylogenetic and sequence analysis of Lumpy skin disease virus circulating in some governorates of Egypt during 2019/2020.

Immune response against lumpy skin disease after simultaneous vaccination of cattle with sheep pox and goat pox and foot and mouth disease vaccines

Foot and mouth disease (FMD) and Lumpy skin disease (LSD) are contagious viral diseases that cause significant economic damage in the livestock industry of countries. Cattle are vaccinated two times a year with FMD and sheep pox and goat pox vaccines (SGP) within 30-day intervals to combat both diseases in Türkiye. However, vaccinations in different periods increase vaccination costs, labor, and distress on animals. Therefore, it was aimed to determine the effects of simultaneous vaccination of FMD and SGP vaccines on the immunity against LSD and FMD in cattle. For this purpose, animals were divided into 4 groups; SGP vaccinated group (Group 1, n = 10), FMD vaccinated group (Group 2, n = 10), FMD and SGP simultaneously vaccinated group (Group 3, n = 10), and the unvaccinated control group (Group 4, n = 6). Blood samples were collected and analyzed to detect the antibody response against the LSD via Capripoxvirus (CaPV) ELISA and FMD by Virus Neutralisation test (VNT) and Liquid Phase Blocking ELISA (LPBE). A live virus challenge study was performed to determine the immune response against LSD. The mean antibody titers were determined protective levels on 28 days post vaccination (DPV) against FMDV serotypes O and A, respectively. The logarithmic difference of skin lesions was calculated log10 titer > 2.5. LSD genome could not be detected in the blood, eyes, and nose swap samples of the challenged animals on the 15th day via PCR. In conclusion, adequate protective immune response was provided against LSD when the SGP and FMD vaccines were used simultaneously in cattle.

Lumpy Skin Disease in Pakistan

The lumpy skin disease virus, also known as LSDV, belongs to the family of Poxviridae and is responsible for lumpy skin disease (LSD). It is a transboundary infection that mainly affects cattle and water buffaloes. Due to its capacity for transboundary transmission, endemic and sporadic outbreaks occur all over the globe, but particularly in Africa and Asia. The virus is primarily mechanically transmitted to susceptible hosts by hematophagous arthropods. The most efficient method to stop the spread of disease is through vaccination in conjunction with other preventative measures. There has been many reports describing these cases in Pakistan in near past and there is a chance that it will spread again [1].&#x0D; In 1929, Zambia recorded the first case of LSD, which later spread to nations in Sub-Saharan Africa, South-Eastern Europe, and Asia. LSD first became known in Asia in Bangladesh, then it expanded to other countries like India, Bhutan, Nepal, Hong Kong, Vietnam, Myanmar, and Thailand. In the Pakistani province of Sindh, district Jamshoro, there was one confirmed case of LSD in 2021 November. When 36,000 infected cattle and a 0.8% mortality rate had been recorded by the end of April 2022 in Sindh, the livestock department proclaimed an epidemic of LSD. A total of five million dairy producers and meat vendors were impacted by the introduction of LSD, which had serious negative economic effects. Additionally, the virus may spread to humans through the milk and meat of sick animals [2].&#x0D; The domesticated animals’ ventures in industrialized countries, including Pakistan, are lingering behind as far as playing it safe against new infections with regards to epidemiological situations and financial requirements. Because of lacking indicative circumstances and viral recognizable proof inside a specific time span, the infection can spread irreversibly and increase the quantity of diseases. Pakistan has never encountered an LSD plague before, however because of endemic LSDV in its neighbors, it faces a high gamble of doing so presently. A combination of vector control, vaccination, stringent quarantine regulations, and livestock mobility restrictions may be successful in halting the spread of the illness.

An Overview on Lumpy Skin Disease: Transmission and Treatments

An Overview on Lumpy Skin Disease: Transmission and Treatments

Development of a dual vaccine against East Coast fever and lumpy skin disease.

East Coast fever is an acute bovine disease caused by the apicomplexan parasite Theileria parva and is regarded as one of the most important tick-vectored diseases in Africa. The current vaccination procedure has many drawbacks, as it involves the use of live T. parva sporozoites. As a novel vaccination strategy, we have constructed the recombinant lumpy skin disease virus (LSDV) named LSDV-SODis-p67HA-BLV-Gag, encoding a modified form of the T. parva p67 surface antigen (p67HA), as well as the bovine leukemia virus (BLV) gag gene for the formation of virus-like particles (VLPs) to potentially enhance p67 immunogenicity. In place of the native sequence, the chimeric p67HA antigen has the human tissue plasminogen activator signal sequence and the influenza hemagglutinin A2 transmembrane domain and cytoplasmic tail. p67HA was detected on the surface of infected cells, and VLPs comprising BLV Gag and p67HA were produced. We also show that higher multiple bands observed in western blot analysis are due to glycosylation of p67. The two vaccines, pMExT-p67HA (DNA) and LSDV-SODis-p67HA-BLV-Gag, were tested for immunogenicity in mice. p67-binding antibodies were produced by vaccinated animals, with higher titers detected in mice vaccinated with the recombinant LSDV. This candidate dual vaccine warrants further testing in cattle.

Detection of Culex tritaeniorhynchus Giles and Novel Recombinant Strain of Lumpy Skin Disease Virus Causes High Mortality in Yaks.

Lumpy skin disease virus (LSDV) is capable of causing transboundary diseases characterized by fever, nodules on the skin, mucous membranes, and inner organs. The disease may cause emaciation with the enlargement of lymph nodes and sometimes death. It has had endemic importance in various parts of Asia in recent years, causing substantial economic losses to the cattle industry. The current study reported a suspected LSDV infection (based on signs and symptoms) from a mixed farm of yak and cattle in Sichuan Province, China. The clinical samples were found positive for LSDV using qPCR and ELISA, while LSDV DNA was detected in Culex tritaeniorhynchus Giles. The complete genome sequence of China/LSDV/SiC/2021 was determined by Next-generation sequencing. It was found that China/LSDV/SiC/2021 is highly homologous to the novel vaccine-related recombinant LSDV currently emerging in China and countries surrounding China. Phylogenetic tree analysis revealed that the novel vaccine-associated recombinant LSDV formed a unique dendrograms topology between field and vaccine-associated strains. China/LSDV/SiC/2021 was found to be a novel recombinant strain, with at least 18 recombination events via field viruses identified in the genome sequence. These results suggest that recombinant LSDV can cause high mortality in yaks, and its transmission might be due to the Culex tritaeniorhynchus Giles, which acts as a mechanical vector.

Cross-Protection of an Inactivated and a Live-Attenuated Lumpy Skin Disease Virus Vaccine against Sheeppox Virus Infections in Sheep.

Sheeppox virus (SPPV) (genus Capripoxvirus, family Poxviridae) infections are a highly virulent and contagious disease of sheep with a high morbidity and mortality, especially in naïve populations and young animals. For the control of SPPV, homologous and heterologous live-attenuated vaccines are commercially available. In our study, we compared a commercially available live-attenuated lumpy skin disease virus (LSDV) vaccine strain (Lumpyvax) with our recently developed inactivated LSDV vaccine candidate regarding their protective efficacy against SPPV in sheep. Both vaccines were proven to be safe in sheep, and neither clinical signs nor viremia could be detected after vaccination and challenge infection. However, the local replication of the challenge virus in the nasal mucosa of previously vaccinated animals was observed. Because of the advantages of an inactivated vaccine and its heterologous protection efficacy against SPPV in sheep, our inactivated LSDV vaccine candidate is a promising additional tool for the prevention and control of SPPV outbreaks in the future.

Annotation of Potential Vaccine Targets and Designing of mRNA-Based Multi-Epitope Vaccine against Lumpy Skin Disease Virus via Reverse Vaccinology and Agent-Based Modeling.

Lumpy skin disease is a fatal emerging disease of cattle, which has started to gain extensive attention due to its rapid incursions across the globe. The disease epidemic causes economic loss and cattle morbidity. Currently, there are no specific treatments and safe vaccines against the lumpy skin disease virus (LSDV) to halt the spread of the disease. The current study uses genome-scan vaccinomics analyses to prioritize promiscuous vaccine candidate proteins of the LSDV. These proteins were subjected to top-ranked B- and T-cell epitope prediction based on their antigenicity, allergenicity, and toxicity values. The shortlisted epitopes were connected using appropriate linkers and adjuvant sequences to design multi-epitope vaccine constructs. Three vaccine constructs were prioritized based on their immunological and physicochemical properties. The model constructs were back-translated to nucleotide sequences and codons were optimized. The Kozak sequence with a start codon along with MITD, tPA, Goblin 5', 3' UTRs, and a poly(A) tail sequences were added to design a stable and highly immunogenic mRNA vaccine. Molecular docking followed by MD simulation analysis predicted significant binding affinity and stability of LSDV-V2 construct within bovine immune receptors and predicted it to be the top-ranked candidate to stimulate the humeral and cellular immunogenic responses. Furthermore, in silico restriction cloning predicted feasible gene expression of the LSDV-V2 construct in a bacterial expression vector. It could prove worthwhile to validate the predicted vaccine models experimentally and clinically against LSDV.

Generation and application of immortalized sertoli cell line from sheep testis

Primary sheep testicular Sertoli cells (STSCs) are ideal for investigating the molecular and pathogenic processes of capripoxvirus. However, the high cost of isolation and culture of primary STSCs, time-consuming operation, and short lifespan greatly limit their real-world application. In our study, the primary STSCs were isolated and immortalized by transfection of a lentiviral recombinant plasmid containing simian virus 40 (SV40) large T antigen. Androgen-binding protein (ABP) and vimentin (VIM) protein expression, SV40 large T antigen activity, proliferation assays, and apoptosis analysis results showed that immortalized large T antigen STSCs (TSTSCs) still had the same physiological characteristics and biological functions as primary STSCs. Moreover, immortalized TSTSCs had strong anti-apoptosis ability, extended lifespan, and enhanced proliferative activity compared to primary STSCs, which had not transformed in vitro and showed any signs of malignancy phenotype in nude mice. Besides, immortalized TSTSCs were susceptible to goatpox virus (GTPV), lumpy skin disease virus (LSDV), and Orf virus (ORFV). In conclusion, immortalized TSTSCs are useful in vitro models to study GTPV, LSDV, and ORFV in a wide range of ways, suggesting that it can be safely used in virus isolation, vaccine and drug screening studies in future.

Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.

The current epidemic in Asia, driven by LSDV recombinants, poses difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of differentiating Neethling-based vaccine strains from classical and recombinant wild-type strains that are currently circulating in Asia. The DIVA potential of this new assay, seen in the in silico evaluation, was confirmed on samples from LSDV infected and vaccinated animals and on isolates of LSDV recombinants (n = 12), vaccine (n = 5), and classic wild-type strains (n = 6). No cross-reactivity or a-specificity with other capripox viruses was observed under field conditions in non-capripox viral stocks and negative animals. The high analytical sensitivity is translated into a high diagnostic specificity as more than 70 samples were all correctly detected with Ct values very similar to those of a published first-line pan capripox real-time PCR. Finally, the low inter- and intra-run variability observed shows that the new DIVA PCR is very robust which facilitates its implementation in the lab. All validation parameters that are mentioned above indicate the potential of the newly developed test as a promising diagnostic tool which could help to control the current LSDV epidemic in Asia.

Lumpy Virus and Skin Infection: A Review

Lumpy skin disease (LSD) responsible for losses in the lives of animals. It is caused due to the virus called Lumpy skin disease virus (LSDV). It belongs to the family Poxviridae and genus Capripoxvirus. It includes sheep pox virus as well as goat pox virus. This disease mainly affect cattle and water buffaloes. The transmission of virus is through the arthropod vectors. it is manifested by characteristics like skin nodules, pyrexia, nasal discharge, swelling of superficial lymph node, lachrymation. The disease is first observed in African and Middle East countries but has started spreading from China and Bangladesh sharing borders with India. LSD causes in female abortions and in male sterility. It can also cause the milk and beef production. This review aims to summaries the latest developments in the epidemiology with the focus on spread, aetiology and transmission, clinical presentations, diagnostics and management of the disease.

Lumpy Skin Disease (LSD): Etiology, Pathogenesis, Prevention and Control

Lumpy skin disease (LSD) is one of the diseases that pose a threat to the livestock industry because it can cause large economic losses. The disease was first discovered in the country of Zambia in 1929. In Indonesia, LSD was first discovered in early 2022 in Indragiri Hulu Regency, Riau. This disease is caused by a virus belonging to the genus Capripoxvirus, subfamily chordopoxvirinae, family Poxviridae. The LSD virus has a limited host and does not infect non-ruminant hosts. A characteristic clinical symptom of LSD is the appearance of nodules on the skin. Diagnosis of this disease can be done by looking at typical clinical symptoms, laboratory tests such as virus isolation, serological tests (serum neutralization test, virus neutralization test (VNT), agar gel immune diffusion, indirect ELISA, and indirect fluorescent antibody technique (IFAT)), real time and conventional PCR, immunohistochemistry, LAMP, and IPMA. Prevention and control of LSD disease can be done in several ways such as vaccination, vector control, restriction of livestock traffic, strict quarantine and stamping out.

Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India

ABSTRACT Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage (P = 50) in Vero cells. The vaccine (50th LSDV passage in Vero cells, named as Lumpi-ProVac Ind ) did not induce any local or systemic reaction upon its experimental inoculation in calves (n = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals (n = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals (n = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.

Can Lumpy Skin Disease be Considered a Zoonosis?

Can Lumpy Skin Disease be Considered a Zoonosis?

Overview of Lumpy Skin Disease in Cattle

Overview of Lumpy Skin Disease in Cattle

Lumpy Skin Disease—An Emerging Cattle Disease in Europe and Asia

Lumpy skin disease virus (LSDV) is a member of the Capripoxvirus genus, mainly infecting cattle and buffalo, which until relatively recently was only endemic in parts of Africa and then spread to the Middle East and lately Europe and Asia. Lumpy skin disease (LSD) is a notifiable disease with a serious impact on the beef industry as it causes mortality of up to 10% and has impacts on milk and meat production, as well as fertility. The close serological relationship between LSDV, goat poxvirus (GTPV) and sheep poxvirus (SPPV) has led to live attenuated GTPV and SPPV vaccines being used to protect against LSD in some countries. There is evidence that the SPPV vaccine does not protect from LSD as well as the GTPV and LSDV vaccines. One of the LSD vaccines used in Eastern Europe was found to be a combination of different Capripoxviruses, and a series of recombination events in the manufacturing process resulted in cattle being vaccinated with a range of recombinant LSDVs resulting in virulent LSDV which spread throughout Asia. It is likely that LSD will become endemic throughout Asia as it will be very challenging to control the spread of the virus without widespread vaccination.

Modelling epidemic growth models for lumpy skin disease cases in Thailand using nationwide outbreak data, 2021-2022.

Lumpy skin disease (LSD) is a transboundary disease affecting cattle and has a detrimental effect on the cattle industries in numerous countries in Africa, Europe and Asia. In 2021, LSD outbreaks have been reported in almost all of Thailand's provinces. Indeed, fitting LSD occurrences using mathematical models provide important knowledge in the realm of animal disease modeling. Thus, the objective of this study is to fit the pattern of daily new LSD cases and daily cumulative LSD cases in Thailand using mathematical models. The first- and second-order models in the forms of Lorentzian, Gaussian and Pearson-type VII models are used to fit daily new LSD cases whereas Richard's growth, Boltzmann sigmoidal and Power-law growth models are utilized to fit the curve of cumulative LSD cases. Based on the root-mean-squared error (RMSE) and Akaike information criterion (AIC), results showed that both first and second orders of Pearson-type VII models and Richard's growth model (RGM) were fit to the data better than other models used in the present study. The obtained models and their parameters can be utilized to describe the LSD outbreak in Thailand. For disease preparedness purposes, we can use the first order of the Pearson-type VII model to estimate the time of maximum infected cases occurring when the growth rate of infected cases starts to slow down. Furthermore, the period when the growth rate changes at a slower rate, known as the inflection time, obtained from RGM allows us to anticipate when the pandemic has peaked and the situation has stabilized. This is the first study that utilizes mathematical methods to fit the LSD epidemics in Thailand. This study offers decision-makers and authorities with valuable information for establishing an effective disease control strategy.

The Lumpy Skin Disease Emergence and Management: A Case Study from the North Indian District of Rewari, Haryana

The Lumpy Skin Disease Emergence and Management: A Case Study from the North Indian District of Rewari, Haryana