Luminous Region Research Articles

Integrity of the Linker of Nucleoskeleton and Cytoskeleton Is Required for Efficient Herpesvirus Nuclear Egress.

Herpesvirus capsids assemble in the nucleus, while final virion maturation proceeds in the cytoplasm. This requires that newly formed nucleocapsids cross the nuclear envelope (NE), which occurs by budding at the inner nuclear membrane (INM), release of the primary enveloped virion into the perinuclear space (PNS), and subsequent rapid fusion with the outer nuclear membrane (ONM). During this process, the NE remains intact, even at late stages of infection. In addition, the spacing between the INM and ONM is maintained, as is that between the primary virion envelope and nuclear membranes. The linker of nucleoskeleton and cytoskeleton (LINC) complex consists of INM proteins with a luminal SUN (Sad1/UNC-84 homology) domain connected to ONM proteins with a KASH (Klarsicht, ANC-1, SYNE homology) domain and is thought to be responsible for spacing the nuclear membranes. To investigate the role of the LINC complex during herpesvirus infection, we generated cell lines constitutively expressing dominant negative (dn) forms of SUN1 and SUN2. Ultrastructural analyses revealed a significant expansion of the PNS and the contiguous intracytoplasmic lumen, most likely representing endoplasmic reticulum (ER), especially in cells expressing dn-SUN2. After infection, primary virions accumulated in these expanded luminal regions, also very distant from the nucleus. The importance of the LINC complex was also confirmed by reduced progeny virus titers in cells expressing dn-SUN2. These data show that the intact LINC complex is required for efficient nuclear egress of herpesviruses, likely acting to promote fusion of primary enveloped virions with the ONM.IMPORTANCE While the viral factors for primary envelopment of nucleocapsids at the inner nuclear membrane are known to the point of high-resolution structures, the roles of cellular components and regulators remain enigmatic. Furthermore, the machinery responsible for fusion with the outer nuclear membrane is unsolved. We show here that dominant negative SUN2 interferes with efficient herpesvirus nuclear egress, apparently by interfering with fusion between the primary virion envelope and outer nuclear membrane. This identifies a new cellular component important for viral egress and implicates LINC complex integrity in nonconventional nuclear membrane trafficking.

The MUSE view of the host galaxy of GRB 100316D

The low distance, $z=0.0591$, of GRB 100316D and its association with SN 2010bh represent two important motivations for studying this host galaxy and the GRB's immediate environment with the Integral-Field Spectrographs like VLT/MUSE. Its large field-of-view allows us to create 2D maps of gas metallicity, ionization level, and the star-formation rate distribution maps, as well as to investigate the presence of possible host companions. The host is a late-type dwarf irregular galaxy with multiple star-forming regions and an extended central region with signatures of on-going shock interactions. The GRB site is characterized by the lowest metallicity, the highest star-formation rate and the youngest ($\sim$ 20-30 Myr) stellar population in the galaxy, which suggest a GRB progenitor stellar population with masses up to 20 -- 40 $M_{\odot}$. We note that the GRB site has an offset of $\sim$660pc from the most luminous SF region in the host. The observed SF activity in this galaxy may have been triggered by a relatively recent gravitational encounter between the host and a small undetected ($L_{H\alpha} \leq 10^{36}$ erg/s) companion.

Generating HDR radiance maps from single LDR image

We present a method of generating high dynamic range (HDR) radiance maps from a single low dynamic range (LDR) image and its camera response function (CRF). The method first models and estimates the inverse CRF and then multiplies the inverse CRF by a weighting function to make it smooth near the maximum and minimum pixel values, and finally conducts the smooth inverse CRF on the input LDR image to generate HDR image. In the method, the inverse CRF is estimated using one single LDR image and an empirical model of CRF, based on measured RGB distributions at color edges. Unlike most existing methods, the proposed method expands image from both high and low luminance regions. Thus, the algorithm can avoid the artifacts and detail loss in dark area which results from extending image only from bright region. Extensive experimental results show that the approach induces less contrast distortion and produces high visual quality HDR image.

The Origin of [C ii] 157 μm Emission in a Five-component Interstellar Medium: The Case of NGC 3184 and NGC 628

Abstract With its relatively low ionization potential, C+ can be found throughout the interstellar medium (ISM) and provides one of the main cooling channels of the ISM via the [C ii] 157 μm emission. While the strength of the [C ii] line correlates with the star formation rate, the contributions of the various gas phases to the [C ii] emission on galactic scales are not well established. In this study we establish an empirical multi-component model of the ISM, including dense H ii regions, dense photon dissociation regions (PDRs), the warm ionized medium (WIM), low density and surfaces of molecular clouds (SfMCs), and the cold neutral medium (CNM). We test our model on ten luminous regions within the two nearby galaxies NGC 3184 and NGC 628 on angular scales of 500–600 pc. Both galaxies are part of the Herschel key program KINGFISH, and are complemented by a large set of ancillary ground- and space-based data. The five modeled phases together reproduce the observed [C ii] emission quite well, overpredicting the total flux slightly (about 45%) averaged over all regions. We find that dense PDRs are the dominating component, contributing 68% of the [C ii] flux on average, followed by the WIM and the SfMCs, with mean contributions of about half of the contribution from dense PDRs, each. CNM and dense H ii regions are only minor contributors with less than 5% each. These estimates are averaged over the selected regions, but the relative contributions of the various phases to the [C ii] flux vary significantly between these regions.

Reconstruction of primary vertices at the ATLAS experiment in Run 1 proton\u2013proton collisions at the LHC

This paper presents the method and performance of primary vertex reconstruction in proton–proton collision data recorded by the ATLAS experiment during Run 1 of the LHC. The studies presented focus on data taken during 2012 at a centre-of-mass energy of sqrt{s} = 8 TeV. The performance has been measured as a function of the number of interactions per bunch crossing over a wide range, from one to seventy. The measurement of the position and size of the luminous region and its use as a constraint to improve the primary vertex resolution are discussed. A longitudinal vertex position resolution of about 30;upmu {text {m}} is achieved for events with high multiplicity of reconstructed tracks. The transverse position resolution is better than 20;upmu {text {m}} and is dominated by the precision on the size of the luminous region. An analytical model is proposed to describe the primary vertex reconstruction efficiency as a function of the number of interactions per bunch crossing and of the longitudinal size of the luminous region. Agreement between the data and the predictions of this model is better than 3% up to seventy interactions per bunch crossing.

Morphology and ultrastructure of the male reproductive system of the jewel wasp, Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae)

The genital systems of insects are undoubtedly of great key for the success of mating and population reproduction. In the meantime, morphological and ultrastructural characteristics of the reproductive systems also provide valuable evidences for the studies of taxonomy, reproductive biology and evolutionary biology. More previously, the reproductive system of female Nasonia vitripennis was already described. To complement the information of reproductive systems from this species, the male reproductive system was here dissected and characterized by light microscopy and transmission electron microscopy. Results showed that the system follows the pattern of most Hymenoptera species: paired testes with one follicle, deferent ducts, seminal vesicles, accessory glands and a fused ejaculatory duct ultimately connected to the external aedeagus. Histologically, the testes, seminal vesicles and accessory glands are all composed of three portions, all of which include epithelial and luminal regions. In addition, both lipidic inclusions and nuclei are presented in the three organs with variable sizes and shapes. Spermatozoa are observed in the testes and seminal vesicles, and also vary considerably in size and shape, suggesting different phases of spermatogenesis. Mitochondria are abundant in seminal vesicles and accessory glands. Notably, membranous inclusions and Golgi complexes are found only in seminal vesicles, whereas secretory granules are presented only in accessory glands, being indicative of organ or age specificity. Together, this study complements the information of the reproductive systems from N. vitripennis, and provides an extensive resource for taxonomy, reproductive biology and evolutionary biology.

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells.

We have characterized the expression and secretion of the acute kidney injury (AKI) biomarkers insulin-like growth factor binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human kidney epithelial cells in primary cell culture and tissue. We established cell culture model systems of primary kidney cells of proximal and distal tubule origin and observed that both proteins are indeed expressed and secreted in both tubule cell types in vitro. However, TIMP-2 is both expressed and secreted preferentially by cells of distal tubule origin, while IGFBP7 is equally expressed across tubule cell types yet preferentially secreted by cells of proximal tubule origin. In human kidney tissue, strong staining of IGFBP7 was seen in the luminal brush-border region of a subset of proximal tubule cells, and TIMP-2 stained intracellularly in distal tubules. Additionally, while some tubular colocalization of both biomarkers was identified with the injury markers kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin, both biomarkers could also be seen alone, suggesting the possibility for differential mechanistic and/or temporal profiles of regulation of these early AKI biomarkers from known markers of injury. Last, an in vitro model of ischemia-reperfusion demonstrated enhancement of secretion of both markers early after reperfusion. This work provides a rationale for further investigation of these markers for their potential role in the pathogenesis of acute kidney injury.

First results from Project SUNBIRD: Supernovae UNmasked By Infra-Red Detection

AbstractA substantial number of core-collapse supernovae (CCSNe) are expected to be hosted by starbursting luminous infrared galaxies (LIRGs). However, so far very few CCSNe have been discovered in LIRGs, most likely as a result of dust extinction and lack of contrast in their typically luminous and complex nuclear regions. We present the first results of Project SUNBIRD (Supernovae UNmasked By InfraRed Detection), where we aim to uncover dust-obscured nuclear supernovae by monitoring over 30 LIRGs, using near-infrared state-of-the-art Laser Guide Star Adaptive Optics (LGSAO) imaging on the Gemini South and Keck telescopes. Such discoveries are vital for determining the fraction of supernovae which will be missed as a result of dust obscuration by current and future optical surveys.

Local intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in decidualized uterus of pseudopregnant rats.

BackgroundCyclooxygenase (COX)-derived prostanoids (PGE2, PGI2) are important contributors to the process of decidualization. Previous studies showed the presence of Ang-(1-7) in the primary and secondary decidualized zones of the implantation site at early pregnancy. Decreased concentrations of Ang-(1-7) were found in the decidualized uterus compared to the non-decidualized uterus of pseudopregnant rats, suggesting that low levels of Ang-(1-7) are required for successful decidualization at early pregnancy.MethodsTo understand the role of Ang-(1-7) in prostaglandin production in a decidualized uterus, induced by a bolus injection of sesame oil, Ang-(1-7) (24 μg/kg/h) or vehicle was then infused directly into the decidualized uterine horn using an osmotic minipump. The right horns were not injected or infused and served as non-decidualized uterine horns in both groups of animals.ResultsDecidualization increased PGE2 concentration in the uterus (0.53 ± 0.05 vs. 12.0 ± 3.2 pmol/mg protein, p < 0.001, non-decidualized vs. decidualized horns); Ang-(1-7) infusion attenuated the increase of PGE2 (12.0 ± 3.2 vs. 5.1 ± 1.3 pmol/mg protein, p < 0.01 control vs. Ang-(1-7) treated decidualized horns). The stable metabolite of PGI2 (6-keto PGF1α) was increased with decidualization (0.79 ± 0.17 vs. 3.5 ± 0.82 pmol/mg protein, p < 0.001, non-decidualized vs. decidualized horns). Ang-(1-7) infusion attenuated the increase in 6-keto PGF1α in the decidualized horn (3.5 ± 0.82 vs 1.8 ± 0.37 pmol/mg protein, p < 0.05 control vs. Ang-(1-7) treated decidualized horns). The circulating levels of 6-keto-PGF1a and TXB2 were decreased by Ang-(1-7) infusion, while no difference was observed in circulating PGE2. Although the global assessment of cleaved caspase 3 immunostaining, a marker of apoptosis, was unchanged within the Ang-(1-7) decidualized horn, there were localized decreases in cleaved caspase 3 staining in the luminal region in the decidualized uterus of Ang-(1-7)-treated rats.ConclusionsThese studies show that increased local uterine Ang-(1-7) alters the uterine prostaglandin environment, possibly leading to disruptions of early events of decidualization.

Mixture fraction, soot volume fraction, and velocity imaging in the soot-inception region of a turbulent non-premixed jet flame

An experimental study is performed to investigate the feasibility of conducting simultaneous mixture fraction, soot volume fraction and velocity imaging in sooting jet flames. The measurements are performed in the soot-inception region of ethylene jet flames, where the yellow luminous region first appears in the flame. Three-component velocity and soot volume fraction are measured by stereoscopic particle image velocimetry and laser-induced incandescence, respectively. The mixture fraction is inferred from laser-induced fluorescence of krypton gas seeded into the fuel stream. To obtain mixture fraction from the fluorescence signal, the signal must be corrected for density and fluorescence quenching effects. This correction is accomplished by invoking an assumed state relationship that is derived from a laminar strained-flame calculation. Once properly calibrated, the krypton planar laser-induced fluorescence data give the mixture fraction, temperature and major species near the regions of soot formation. The krypton is seeded into the fuel jet at a mole fraction of approximately 4%. The fluorescence of krypton is achieved by two-photon absorption at 214.7nm and the resulting fluorescence is collected at 760.2nm. The krypton fluorescence signal is rather weak, particularly near the reaction zones where density is lowest, and so adequate signal-to-noise ratios could be achieved in a region only about 1mm in height, which effectively limited this study to a line measurement of mixture fraction. The temperature field derived from the mixture fraction field was compared to temperatures obtained from thermocouple measurements. The mean radial temperature profiles using the different techniques show excellent agreement and this serves to validate the methodology used to map from fluorescence signal to mixture fraction and temperature. The resulting data are of high enough quality as to allow the investigation of the kinematics, thermo-chemical state and even the dissipation fields near regions of soot formation.

In-cylinder combustion visualization of Jatropha straight vegetable oil and mineral diesel using high temperature industrial endoscopy for spatial temperature and soot distribution

Endoscopic optical visualization technique is reliable for investigations in harsh environments such as IC engine combustion chambers. In this study, high temperature industrial endoscopy was employed to a production grade diesel engine for optical diagnostics of in-cylinder combustion. Engine cylinder-head was suitably modified for visual access of combustion. Combustion characteristics such as in-cylinder pressure, heat release rate (HRR), start of combustion (SoC), combustion duration (CD), etc., for Jatropha straight vegetable oil (SVO) and mineral diesel were investigated using Industrial endoscope, which can withstand such harsh environment. In-cylinder combustion images were recorded using a high-speed charge coupled device (CCD) camera. Recorded images were corrected using calibration tool to remove endoscopic lens distortions (spherical aberration). Spatial soot and flame temperature distributions were analysed from the corrected combustion images using soot analysis and correlated colour temperature (CCT) methods. Spatial soot and temperature distributions for the test fuels indicated that luminous regions in the combustion chamber were the prime locations for soot formation. These regions also indicated peak temperature zones in the field of view. This study is unique, where in-situ combustion characteristics were determined for SVO and baseline diesel using endoscopic visualization. This study generated valuable information about in-cylinder spatial combustion of SVO, which can be utilized for combustion and emission optimization of SVO fuelled engines.

The massive stellar population of W49: A spectroscopic survey

Massive stars form on different scales ranging from large, dispersed OB associations to compact, dense starburst clusters. The complex structure of regions of massive star formation, and the involved short timescales provide a challenge for our understanding of their birth and early evolution. As one of the most massive and luminous star-forming region in our Galaxy, W49 is the ideal place to study the formation of the most massive stars. By classifying the massive young stars deeply embedded into the molecular cloud of W49, we aim to investigate and trace the star formation history of this region. We analyse near-infrared $K$-band spectroscopic observations of W49 from LBT/LUCI combined with $JHK$ images obtained with NTT/SOFI and LBT/LUCI. Based on $JHK$-band photometry and K-band spectroscopy the massive stars are placed in a Hertzsprung Russell diagram. By comparison with evolutionary models, their age and hence the star formation history of W49 can be investigated. Fourteen O type stars as well as two young stellar objects (YSOs) are identified by our spectroscopic survey. Eleven O-stars are main sequence stars with subtypes ranging from O3 to O9.5, with masses ranging from $\sim 20$ $M_{\odot}$ to $\sim 120$ $M_{\odot}$. Three of the O-stars show strong wind features, and are considered to be Of-type supergiants with masses beyond 100 $M_{\odot}$ . The two YSOs show CO emission, indicative for the presence of circumstellar disks in the central region of the massive cluster. The age of the cluster is estimated as $\sim1.5$ Myr, with star formation still ongoing in different parts of the region. The ionising photons from the central massive stars have not yet cleared the molecular cocoon surrounding the cluster. W49 is comparable to extragalactic star-forming regions and provides us with an unique possibility to study a starburst in detail.

Two modes of a plasma jet excited by a direct current voltage

A plasma jet excited by a direct current voltage is developed to generate a diffuse plasma plume by blowing atmospheric pressure argon. Results show that the plume discharge operates in a single-pulsed mode or a continuous one depending on the applied voltage. For the single-pulsed mode, the discharge frequency increases with increasing the applied voltage or the air concentration, while it keeps almost constant with increasing the argon flow rate. The discharge dynamics at the breakdown stage indicate that the light emission propagates along the gas flow at a velocity in the order of 104 m s−1. The spatially resolved emission intensity at the afterglow stage of the pulsed discharge manifests a stratification into dark and bright luminous regions along the gas flow. For the continuous mode, however, the emission intensity gradually decreases along the gas flow. It is found that the continuous discharge is in a Townsend discharge regime judged from both the positive slope of the voltage–current curve and the small current density on the cathode surface. Based on optical emission spectroscopy, excited electron temperature and gas temperature of the plasma plume are obtained by a Boltzmann plot and fitting the spectra of OH radicals, respectively.

Ultra‐high‐resolution 1058‐ppi OLED displays with 2.78‐in size using CAAC‐IGZO FETs with tandem OLED device and single OLED device

AbstractWe fabricated new 2.78‐in 1058‐ppi organic light‐emitting diode (OLED) displays. The displays used OLED devices with a tandem structure and a single structure and a field effect transistor (FET) using c‐axis aligned crystalline In–Ga–Zn–O (CAAC‐IGZO) for an active layer and employing the 1.5‐µm rule over a glass substrate. Even in the displays with such high resolution exceeding 1000 ppi, crosstalk that was observed in the lower luminance region was suppressed. The displays achieved high color reproducibility and reduced viewing angle dependence.

Tubular aggregate myopathy caused by a novel mutation in the cytoplasmic domain of STIM1.

Objective:To identify the gene mutation of tubular aggregate myopathy (TAM) and gain mechanistic insight into the pathogenesis of the disorder.Methods:We described a family affected by autosomal dominant TAM and performed exome and Sanger sequencing to identify mutations. We further analyzed the functional significance of the identified mutation by expression studies and intracellular Ca2+ measurements.Results:A 42-year-old man presented with slowly progressive muscle weakness and atrophy in all 4 limbs and the trunk. Muscle biopsy and microscopic examination revealed tubular aggregates in his skeletal muscle. Genetic analysis of this family identified a novel heterozygous mutation, c.1450_1451insGA (p.Ile484ArgfsX21), in stromal interaction molecule 1 (STIM1), a Ca2+ sensor in sarcoplasmic reticulum. We transfected cultured cells with STIM1 and demonstrated that the mutant STIM1 exhibited aggregation-like appearance in shrunk cytoplasm. Furthermore, we revealed that the intracellular Ca2+ influx is decreased by the mutant STIM1.Conclusions:The novel mutation p.Ile484ArgfsX21 is located in the cytoplasmic C-terminal inhibitory domain (CTID) of STIM1. However, all mutations reported so far in TAM reside in the luminal N-terminal EF hand region. The aggregation-like appearance of STIM1 and the decreased intracellular Ca2+ influx in cells transfected with CTID mutant are in sharp contrast to these previous reports. Taken together, these findings indicate that mutations of STIM1 cause TAM through the dysregulation of Ca2+ homeostasis.

Cardiac ryanodine receptor: Selectivity for alkaline earth metal cations points to the EF-hand nature of luminal binding sites

A growing body of evidence suggests that the regulation of cardiac ryanodine receptor (RYR2) by luminal Ca2+ is mediated by luminal binding sites located on the RYR2 channel itself and/or its auxiliary protein, calsequestrin. The localization and structure of RYR2-resident binding sites are not known because of the lack of a high-resolution structure of RYR2 luminal regions. To obtain the first structural insight, we probed the RYR2 luminal face stripped of calsequestrin by alkaline earth metal divalents (M2+: Mg2+, Ca2+, Sr2+ or Ba2+). We show that the RYR2 response to caffeine at the single-channel level is significantly modified by the nature of luminal M2+. Moreover, we performed competition experiments by varying the concentration of luminal M2+ (Mg2+, Sr2+ or Ba2+) from 8mM to 53mM and investigated its ability to compete with 1mM luminal Ca2+. We demonstrate that all tested M2+ bind to exactly the same RYR2 luminal binding sites. Their affinities decrease in the order: Ca2+>Sr2+>Mg2+~Ba2+, showing a strong correlation with the M2+ affinity of the EF-hand motif. This indicates that the RYR2 luminal binding regions and the EF-hand motif likely share some structural similarities because the structure ties directly to the function.

Concordance of KRAS mutation status between luminal and peripheral regions of primary colorectal cancer. A laser-capture microdissection-based study.

The presence of KRAS mutation in colorectal cancer (CRC) is a marker of resistance to anti-EGFR therapy. However, there are conflicting reports concerning intratumoral heterogeneity of KRAS mutations. The aim of this study was to determine whether within primary CRCs with KRAS mutations intratumoral KRAS mutation heterogeneity can be detected between two strictly defined areas, i.e. the luminal (mucosa/submucosa) and peripheral invasive front of the tumor. Using laser-capture microdissection, from every tumor about 400-500 nests of cancer cells were excised from each of the examined areas (luminal and peripheral) and PNAClamp, a high-sensitivity real-time PCR-based diagnostic assay for KRAS mutation testing, was used for molecular analysis. KRAS mutations were detected in codon 12 in both luminal and peripheral regions in all tumors examined. We conclude that from the point of view of practical KRAS mutation testing for predictive purposes in patients with CRC (i.e. testing mutations in codons 12 and 13) sampling errors are unlikely to occur if in CRCs with KRAS mutations only the luminal (as in biopsy tissue) or peripheral region is examined, provided a sensitive system of detection is applied and an appropriate number of tumor cells with minimal contamination by benign cells is analyzed.

Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteins.

The introduction of pHluorin, a pH-sensitive GFP, by Miesenbock and colleagues provided a versatile tool to studies of vesicle trafficking, in particular synaptic vesicle exocytosis and endocytosis. By tagging pHluorin to the luminal region of the synaptic vesicular protein synaptobrevin (also called VAMP, vesicle-associated membrane protein) or other synaptic vesicle-specific proteins such as the vesicular glutamate transporter-1, we are able to directly track synaptic vesicle endocytosis in response to stimuli in a molecularly specific manner. Here, we describe the process of imaging synaptic vesicle endocytosis in response to extracellular stimulation in dissociated neuronal cultures of hippocampal neurons obtained from rats-also applicable to mice-using pHluorin-tagged vesicular glutamate transporter-1 as a reporter.

Characterization of Ca(2+)-Dependent Protein-Protein Interactions within the Ca(2+) Release Units of Cardiac Sarcoplasmic Reticulum.

In the heart, excitation-contraction (E-C) coupling is mediated by Ca2+ release from sarcoplasmic reticulum (SR) through the interactions of proteins forming the Ca2+ release unit (CRU). Among them, calsequestrin (CSQ) and histidine-rich Ca2+ binding protein (HRC) are known to bind the charged luminal region of triadin (TRN) and thus directly or indirectly regulate ryanodine receptor 2 (RyR2) activity. However, the mechanisms of CSQ and HRC mediated regulation of RyR2 activity through TRN have remained unclear. We first examined the minimal KEKE motif of TRN involved in the interactions with CSQ2, HRC and RyR2 using TRN deletion mutants and in vitro binding assays. The results showed that CSQ2, HRC and RyR2 share the same KEKE motif region on the distal part of TRN (aa 202–231). Second, in vitro binding assays were conducted to examine the Ca2+ dependence of protein-protein interactions (PPI). The results showed that TRN-HRC interaction had a bell-shaped Ca2+ dependence, which peaked at pCa4, whereas TRN-CSQ2 or TRN-RyR2 interaction did not show such Ca2+ dependence pattern. Third, competitive binding was conducted to examine whether CSQ2, HRC, or RyR2 affects the TRN-HRC or TRN-CSQ2 binding at pCa4. Among them, only CSQ2 or RyR2 competitively inhibited TRN-HRC binding, suggesting that HRC can confer functional refractoriness to CRU, which could be beneficial for reloading of Ca2+ into SR at intermediate Ca2+ concentrations.

Alignment of the ATLAS Inner Detector Upgraded for the LHC Run II

ATLAS is equipped with a tracking system built using different technologies, silicon planar sensors (pixel and micro-strip) and gaseous drift-tubes, all embedded in a 2T axial magnetic field. For the LHC Run II, the tracking system has been upgraded with the installation of a new pixel layer, the Insertable Barrel Layer (IBL). An outline of the track based alignment approach and its implementation within the ATLAS software will be presented. Special attention will be paid to integration in the alignment framework of the IBL, which plays a key role in precise reconstruction of the collider luminous region, interaction vertices and identification of long-lived heavy flavour states. In order to detect as soon as possible deformations and misalignments of the tracking system that may affect the data taking, a fast alignment chain was implemented at the Tier-0 at CERN. Latest upgrades and tests of this fast chain will be discussed, as well as the performance from the Cosmic Ray commissioning run.