Luminal Formation Research Articles

Progress in the creation of long-lived atmospheric luminous formations in a pulsed electric discharge with an electrolytic electrode

This work presents an analysis of the research conducted in many countries in recent years on the so-called Gatchina discharge. The findings indicate that the Gatchina discharge exhibits the majority of the characteristics of natural ball lightning, making it the most effective method for reproducing and studying this phenomenon. To a large extent, our new results are based on experiments performed for the first time to visualize dust particles arising in an erosive emission, as well as the formation of vortex flows. These experiments make it possible to explain the ability of the Gatchina discharge to maintain its shape for a long time in the afterglow.

Primary Cutaneous NUT Carcinoma: Clinicopathologic and Genetic Study of 4 Cases.

WHO Classification of Skin Tumors, fifth edition (2023) has newly described primary cutaneous NUT carcinoma; however, information on this cancer type remains scarce. Herein, we performed clinicopathologic and genetic analyses of 4 cases. Four elderly women (median age 77y, range: 68 to 82y) were included. The median tumor size was 12.5 (10 to 40mm). Tumors were located on the scalp, temple, thigh, and palm. Two (50%) patients presented with regional lymph node metastases. Neither distant metastasis nor mortality was observed during patient follow-up of 10.5 (3 to 15) months. Sanger, panel DNA and whole-exome RNA sequencing revealed BRD3::NUTM1 (n=2) and BRD4::NUTM1 (n=2) fusions. Histology of BRD3 -rearranged tumors revealed an epidermal connection, relatively small tumor nests, and ductal or intracytoplasmic luminal formation, whereas that of BRD4 -rearranged tumors revealed large solid nests comprising discohesive tumor cells. NUT, cytokeratins, p63, EMA, TRPS1, c-MYB, CD56, and INSM1 were immunoexpressed to varying degrees in all (100%) tumors. Furthermore, diffuse SOX10 expression was common (3/4, 75%). The literature review of five previously described cases revealed women predominance, no recurrence, frequent BRD3::NUTM1 fusions, and histology of ductoglandular structures. Our study findings and literature suggest elderly women predominance, relatively frequent BRD3::NUTM1 fusions, histopathologic ductoglandular differentiation, absence of abrupt keratinisation, and a characteristic immunoprofile in primary cutaneous NUT carcinoma, unlike in that of other organ. No distant metastasis or disease-associated mortality was seen in all cases with limited follow-up.

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix.

Ultrashort self-assembling peptides (SAPs) can spontaneously form nanofibers that resemble the extracellular matrix. These fibers allow the formation of hydrogels that are biocompatible, biodegradable, and non-immunogenic. We have previously proven that SAPs, when biofunctionalized with protein-derived motifs, can mimic the extracellular matrix characteristics that support colorectal organoid formation. These biofunctional peptide hydrogels retain the original parent peptide's mechanical properties, tunability, and printability while incorporating cues that allow cell-matrix interactions to increase cell adhesion. This paper presents the protocols needed to evaluate and characterize the effects of various biofunctional peptide hydrogels on cell adhesion and lumen formation using an adenocarcinoma cancer cell line able to form colorectal cancer organoids cost-effectively. These protocols will help evaluate biofunctional peptide hydrogel effects on cell adhesion and luminal formation using immunostaining and fluorescence image analysis. The cell line used in this study has been previously utilized for generating organoids in animal-derived matrices.

Microwave diagnostics of pulsed atmospheric discharge with electrolytic electrode and long‐lived luminous formation in its afterglow

AbstractThe Gatchina discharge phenomenon holds significant promise as a laboratory model for simulating ball lightning. However, crucial aspects concerning the plasma components of the resulting afterglow remain unresolved. Notably, the measurement of the electron density, a critical parameter, has not been fully achieved thus far. In this study, microwave diagnostics and video recording were employed during a pulsed Gatchina discharge, along with synchronous measurement of discharge current and voltage. Distinct antennas were positioned at different heights to enable separate diagnosis of the discharge and the ensuing long‐lived afterglow. The findings revealed that during the active phase of the Gatchina discharge, the plasma density was substantial enough to cause reflection of an electromagnetic wave with a frequency of 20 GHz from this highly conductive object. In the afterglow, the signal experienced only a moderate weakening of 10–20 percent, facilitating the determination of the time dependence of average electron density during the afterglow's passage between the two antennas. These measurements verified the unusually slow plasma decay in the afterglow of the Gatchina discharge, suggesting the potential significance of chemi‐ionisation processes involving long‐lived (metastable) particles.

Histological and immunohistochemical prognostic factors of primary angiosarcoma.

Angiosarcoma is a malignant vascular endothelial neoplasm with various histological patterns. Despite its highly malignant potential, histological prognostic prediction has not been adopted for angiosarcoma. This study aimed to establish a method of predicting the prognosis of primary angiosarcoma. Formalin-fixed, paraffin-embedded samples from 104 primary angiosarcomas were prepared. All the cases were reviewed based on histological examinations with H&E staining. Because the French Fédération Nationale des Centres de Lutte Contre Le Cancer system (FNCLCC) is not adopted for angiosarcoma, we experimentally established a modified version of FNCLCC. Immunohistochemical staining for ERG, CD31, CD34, D2-40, HHV-8, p16, C-MYC, and p53 was performed. Fluorescence in situ hybridization (FISH) was performed for 31 cases to assay c-MYC gene amplification. Multivariate analysis revealed that age (> 70years old) (p = 0.0011), non-cutaneous angiosarcoma (p = 0.0265), metastasis on diagnosis (p < 0.0001), size ≥ 5cm (p = 0.0388), no taxane chemotherapy (p = 0.0388), strong nuclear atypia (p = 0.0087), and the presence of luminal structure in ≥ 50% of the tumor volume (p = 0.0009) were independent poor prognostic factors. Among angiosarcomas with luminal formation, mFNCLCC scores were significantly correlated with a poorer prognosis. The overexpression of p16 was associated with less luminal formation (p = 0.0192). Immunohistochemical analysis of C-MYC showed a moderate level of concordance with FISH (Kappa value = 0.45). This study suggested that luminal formation and nuclear atypia may be poor histological prognostic factors of angiosarcoma and that mFNCLCC would be useful for predicting the prognosis of angiosarcoma with luminal formation.

Unifying theory of carotid plaque disruption based on structural phenotypes and forces expressed at the lumen/wall interface

ObjectivesTo integrate morphological, haemodynamic and mechanical analysis of carotid atheroma driving plaque disruption.Materials and methodsFirst, we analysed the phenotypes of carotid endarterectomy specimens in a photographic dataset A, and matched...

Adverse Effects of High Temperature On Mammary Alveolar Development In Vitro.

In the mammary glands during pregnancy, the alveolar buds are first branched from the mammary ducts after which they form the alveolar luminal structure for milk production postparturition. Body temperature could increase for several reasons, such as infectious disease and heat stress. We have previously reported that high temperature adversely effects on the lactation capacity of mouse mammary epithelial cells (MECs). However, it remains unclear how high temperature influences mammary morophogenesis during pregnancy. In this study, we investigated the effects of high temperature on this mammary alveolar development process using two types of culture models including embedded organoids of MECs in Matrigel; these models reproduced mammary alveolar bud induction and alveolar luminal formation. Results showed that a culture temperature of 41°C repressed alveolar bud induction and inhibited alveolar luminal formation. In addition, the treatment at 41°C decreased the number of proliferating mammary epithelial cells but did not affect cell migration. Levels of phosphorylated Akt, -ERK1/2, -HSP90, and -HSP27 were increased in organoids cultured at 41°C. The specific inhibitors of HSP90 and HSP27 exacerbated the disruption of organoids at 41°C but not at 37°C. Furthermore, the organoids precultured at 37 and 41°C in the alveolar luminal formation model showed differences in the expression levels of caseins and tight junction proteins, which express in MECs in lactating mammary glands, after induction of MEC differentiation by prolactin and dexamethasone treatment in vitro. These results suggest that elevated temperature directly hinders mammary alveolar development; however, heat shock proteins may mitigate the adverse effects of high temperatures.

Effect of Jianpi Huogu Formula on function damage of vascular endothelial cells induced by glucocorticoid

This study aimed to observe the intervention effect of Jianpi Huogu Formula(JPHGF) on the functional damage of vascular endothelial cells caused by glucocorticoid, and explore its action mechanism from the PI3 K/Akt and mitogen activated protein kinase(MAPK) signaling pathways. The extracted thoracic aorta ring of normal SD rats were intervened first with vascularendothelial growth factor(VEGF, 20 μg·L-1) and/or sodium succinate(MPS, 0. 04 g·L-1) in vitro and then with JPHGF(8, 16, and 32 μg·L-1) for five mcontinuous ethylpdays, rednisolofollowed nebythe statistics of the number, length, and area of microvessels budding fromvascular rings. In addition, the human umbilical vein endothelial cells(HUVECs) induced by VEGF(20 μg·L-1) were added with MPS(0. 04 g·L-1) and then with JPHGF(8, 16, and 32 μg·L-1) for observing the migration, invasion, and luminal formation abilities of HUVECs in the migration, invasion and luminal formation experiments. The protein expression levels of PI3 K, p-Akt, p-JN K, and p-ERK in HUVECs were assayed by Western blot. The results showed that JPHGF dose-dependently improved the num-ber,length, and area of microvessels in MPS-induced rat thoracic aortic ring, reversed the migration, invasion and lumen formation abiliti es of HUVECs reduced by MPS, and up-regulated the protein expression levels of PI3 K, p-Akt, and p-JNK in HUVECs. All thesehave suggested that JPHGF exerts the protective effect against hormone-induced damage to the angiogenesis of vascular endothelial cells by activating the PI3 K/Akt and MAPK signaling pathways, which has provided reference for exploring the mechanism of JPHGF in treating s teroid-induced avascular necrosis of femoral head(SANFH) and also the experimental evidence for enriching the scientific connotationof spleen-invigorating and blood-activating therapy.

Влияние магнитных и электрических полей на динамику образования плазмоидов в гатчинском разряде

The behavior in magnetic and electric fields of the Gatchina discharge, which is used mainly to create an analog of ball lightning in the laboratory in a normal atmosphere, is analyzed. Shown that in these studies it is possible to determine the sign of an uncompensated electric charge as in the active phase of the discharge, and in the forming long-lived luminous formations. Also shown that electric and magnetic fields can change the direction of movement of the forming luminous formation and even completely block its formation. The type and mechanism of existence firework ball lightning are considered, photos of which are presented in widely known monographs on the ball lightning.

Abstract P107: Celastrol Supplementation Profoundly Activates Aortic Mmp-9 And Abolishes Sexual Dimorphism Of Abdominal Aortic Aneurysm In Mice.

Background and Objective: Abdominal Aortic Aneurysms (AAAs) are permanent dilations of the abdominal aorta with greater than 80% mortality after rupture. AAA prevalence is 4-5 times greater in males than females. AAA formation involves a complex process of destruction of aortic media through degradation of extracellular matrix proteins, elastin and collagen. Angiotensin II (AngII) infusion model of AAA in mice recapitulates major features of human AAA mainly male gender specificity. Celastrol, a pentacyclic triterpene from the root extracts of Thunder God Vine ( Tripterygium wilfordii ), strongly suppressed AngII-induced cardiovascular complications in mice. The purpose of this study is to test the effect of Celastrol supplementation on AngII-induced AAAs in mice. Methods and Results: Male and female LDL receptor -/- mice (8 weeks old; n= 12 per group) were fed a fat-enriched diet (21% wt/wt fat; 0.15% wt/wt cholesterol) supplemented with or without Celastrol (10mg/kg/day) for 5 weeks. After 1 week of diet feeding, mice were infused with AngII (500 or 1000 ng/kg/min) for 28 days by osmotic minipumps. Dietary supplementation of celastrol significantly promoted AngII-induced abdominal aortic luminal dilation (Con = 1.25 ± 0.05 versus Celas = 1.55 ± 0.09 mm, P&lt;0.05) and external aortic width (Con= 1.01 ± 0.09 versus Celas = 1.62 ± 0.14 mm, P&lt;0.05) in male mice as measured by ultra-sonography and ex vivo measurement, with 90% incidence (10/11) compared to 36% (4/11) in control group. Interestingly, celastrol supplementation to AAA female mice, resulted in a significant increase in AngII-induced aortic luminal dilation (Con = 1.17 ± 0.30 versus Celas = 1.57 ± 0.21 mm, P&lt;0.001) and AAA formation (Con= 0.94 ± 0.03 versus Celas = 1.36 ± 0.25 mm, P&lt;0.05) mice with 80% incidence (12/15) compared to 6% (1/15) in control group. Celastrol supplementation dramatically increased AngII-induced aortic leukocyte accumulation, MMP-9 activity and medial elastin degradation in both male and female mice compared to saline and AngII controls. Conclusion: These findings demonstrate that celastrol supplementation to LDLr -/- mice ablates sexual dimorphism and promotes AngII-induced AAA formation, which is associated with increased leukocytic infiltration and MMP-9 activation.

Testosterone exposure in prenatal life disrupts epithelial nuclear morphology, smooth muscle layer pattern, and FGF10 and Shh expression in prostate

The aim of this study was to evaluate whether high levels of exogenous testosterone (T) interfere in prostate morphogenesis. Pregnant females were exposed to subcutaneous injections of T cypionate (500 μg/animal) at gestational days 20 and 22. Male and female pups were euthanized at postnatal days 1 and 15. 15-day-old males had only fibroblast growth factor 10 (FGF10) immunostaining and nuclear form factor altered by the treatment, whereas treated females (T1 and T15) had almost all analyzed parameters changed. T1 females showed an increased anogenital distance (AGD), whereas T15 females had both AGD and ovary weight increased. T1 females had a higher number of epithelial buds emerging from the urethral and vaginal epithelium. We observed ectopic prostatic tissue surrounding the vagina in both T1 and T15 females. Moreover, the ectopic acini of T15 females showed delayed luminal formation, and there was a thickening of the periacinar smooth muscle layer (SML). Finally, FGF10 immunostaining intensity decreased in both T15 male and female prostates. Indeed, Sonic hedgehog (Shh) was upregulated in T15 female prostates, whereas no difference was observed between the male groups. These data showed that exogenous T changed the nuclear morphology of prostate epithelial cells in both males and females. Surprisingly, smooth muscle hyperplasia was also observed in the ectopic female prostate. Moreover, T downregulated FGF10 in both male and female prostates. Interestingly, the results suggest that FGF10 downregulation is mediated by the upregulation of Shh in females. In conclusion, exogenous T disrupts prostate development, particularly, affecting, the female.

Histopathology of the Foot, Gill and Digestive Gland Tissues of Freshwater Mussel, Lamellidens marginalis Exposed to Oil Effluent

We investigated the histopathological alterations in the tissues of freshwater mussel, Lamellidens marginalis in response to oil effluent. Based on the previous acute toxicity, two sub lethal [1/4th (11.88ppt) and 1/10th (8.55ppt)] concentrations of oil effluent (hydrocarbon) were prepared and exposed to mussels. In a first series of experiment, animals were exposed/accumulated for 30 days [Ist, 8th, 15th, 22nd and 30th days] by two sub lethal concentrations of oil. In a second series of experiment, oil exposed animals were thereafter transferred to clean water and kept in it up to 30 days [Ist, 8th, 15th, 22nd and 30th days] to assess the recovery pattern (depuration). At seven-day intervals, histopathological alterations were analyzed in foot, gill and digestive gland tissues of mussel. First series of experimental observation showed remarkable damages in foot (disorganized outer epithelium, necrosis of the cell, the formation of lumina, disorganized muscle bundle); in gill (disruption of gill filaments, odema formation, necrosis, dis-aggregated cilia) and in digestive gland (stoma, detached glandular epithelium, vertical clefts, presence of leucocytes, dense accumulation of luminal material) and also oil effluent inducement are confirmed with the aforementioned results. At second series of experiment, it was found that oil effluent tended to accumulate in tissues in a duration-dose-dependent manner. Tissue burden by oil effluent of mussels completely were restored at 30th day. The present experimental findings may be of early warning signals of oil effluent pollution. In conclusion oil effluent are highly toxic to the Lamellidens marginalis.

Исследование динамики формирования плазмоидов в гатчинском разряде

The results of studies of the Gatchina discharge carried out at the Harbin Technological University (China) are presented. The Gatchina discharge is mainly used to create an analogue of ball lightning in the ordinary atmosphere of a laboratory. Most of the research on this discharge by various groups of scientists is devoted to the study of the first phase of the discharge, when an electric discharge is galvanically coupled to an electrode. This is due to the fact that many researchers consider this phase of the discharge to be decisive for the subsequent long-term existence of an autonomous luminous formation - the second phase of the discharge. In this paper, in comparison with similar works, the leader-streamer process on the water surface was demonstrated more fully and with a better resolution. The use of the filter made it possible to better show the role of leaders, streamers, and the ionization region in the immediate vicinity of the water surface and to clarify some of the processes occurring in the autonomous phase of the discharge. In the wavelength range of 400-500 nm, the autonomous luminous formation showed a longer lifetime in the form of a spherical shape than when observed in "white" light. With a sharp increase in the amount of substance introduced to the cathode, a new physical effect was recorded, which consists in the fact that in the medium of an ordinary atmosphere, a complex formation of a white color is formed without losing contact with the electrode (cathode). By varying the electrical conductivity and initial voltage, an unexpectedly small luminous formation with a diameter of 15-17 mm was obtained for the first time, which was not previously observed in a Gatchina discharge. Key words: Gatchina discharge, plasmoid, long-lived luminous formations, plasma sheet, space charge, virtual cathode, ball lightning. By varying the electrical conductivity and the initial voltage, an unexpectedly small luminous formation with a diameter of 15-17 mm was obtained for the first time, which was not previously observed in the Gatchina discharge.

Application of “Tissueoid Cell Culture System” Using a Silicate Fiber Scaffold for Cancer Research

Background: We developed a 3-dimensional (3D) culture system using a high-purity silica fiber scaffold of unwoven sheets called Cellbed^TM. Methods: We used adherent colon and esophagogastric junction adenocarcinoma cells, tongue squamous cell carcinoma (SqCC) cells, and nonadherent gastric cancer cells. These cells were subjected to staining with various substances and observed by electron microscopy. To evaluate the effects of extracellular matrix in carcinoma tissues, SqCC cells were cultured in Cellbed coated with collagens I, III, and IV. Results: Especially well-differentiated carcinoma cells cultured in this 3D system showed their own unique characteristics: luminal formation in adenocarcinoma cells and cell stratification and keratinization in SqCC cells. Scanning electron microscopy revealed the proliferation of cancer cells with cytoplasm entwined in Cellbed. Intercellular desmosomes in squamous epithelia were detected by transmission electron microscopy of vertical cross sections. SqCC cells cultured in Cellbed coated with collagen IV showed enhanced invasive and proliferative abilities. Conclusion: Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a “tissueoid cell culture system.” Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.

ADENOID CYSTIC CARCINOMA ON THE BASIS OF TONGUE: A RARE CASE REPORT

A 55-year-old male patient attended a dermatology center because of dysarthria and dysphagia. The clinical examination showed swelling in the base of the tongue extending bilaterally to the borders preserving the median longitudinal groove. The patient underwent punch biopsy, and the specimen was referred for anatomopathologic examination with clinical suspicion of hypertrophy of tongue. Histopathologic examination revealed malignant neoplasm of glandular origin composed of cells with scarce eosinophilic cytoplasm and large hyperchromatic nuclei, organized in nests and cords sometimes with cribriform pattern and luminal formation filled with homogeneous eosinophilic material. The morphologic characteristics suggested a diagnosis of adenoid cystic carcinoma (ACC) or metastatic tumor. The immunohistochemical study demonstrated that epithelial/ductal cells were positive for cytokeratins and basal cells for p63 protein revealing their myoepithelial origin, with membrane epithelial antigen being focally positive, which led to the diagnosis of ACC. The patient was referred to an oncologic reference center.

Scientific goals of optical instruments of the National Heliogeophysical Complex

Studies of the upper atmosphere have to be performed using optical photometric and spectrometric means. Modern devices allow precise photometry of the glow of the night atmosphere — airglow — with high temporal spatial and spectral resolution. As a result, the obtained airglow parameters make it possible to determine the physicochemical properties of the upper atmosphere and observe their variation under the influence of various factors. The National Heliogeophysical Complex, which is being created in Eastern Siberia, is therefore to include a certain set of modern optical instruments. The paper presents the main phenomena that will be investigated by the optical instruments of the complex, provides information on their composition and scientific goals, presents the results of preliminary studies performed using a prototype of the instruments. As a result of the studies, the presence of a significant (about 10 m/sec) vertical wind at various altitudes (100 and 250 km) was established, the importance of taking into account the vertical wind to study the vertical dynamics of the charged component was demonstrated. The long-term dynamics of the vertical wind at an altitude of about 100 km has a pronounced seasonal variations and the absence of diurnal variations, whereas the dynamics of the vertical wind at an altitude of 250 km has a pronounced diurnal variations, which is mostly clearly defined in winter. This suggests the presumed presence of vertical circulation cells at various altitude levels. The possibilities of optical stereoscopy and differential image analysis methods are demonstrated, as applied to the study of fast luminous formations and conducting active ground and space experiments to modify Earth's ionosphere. We report the results of the determination of a three-dimensional picture of a long-lived meteor track with the use of two wide-angle cameras. We propose an algorithm that allows us to get a stereo image of events occurring in the upper atmosphere, recorded simultaneously from different observation points. The joint work of the tools of this complex and the development of cooperation with third-party organizations are shown to be a good enough direction for further study of the vertical dynamics of Earth’s upper atmosphere and space weather phenomena.

Научные задачи оптических инструментов Национального гелиогеофизического комплекса

Studies of the upper atmosphere have to be performed using optical photometric and spectrometric means. Modern devices allow precise photometry of the glow of the night atmosphere — airglow — with high temporal spatial and spectral resolution. As a result, the obtained airglow parameters make it possible to determine the physicochemical properties of the upper atmosphere and observe their variation under the influence of various factors. The National Heliogeophysical Complex, which is being created in Eastern Siberia, is therefore to include a certain set of modern optical instruments. The paper presents the main phenomena that will be investigated by the optical instruments of the complex, provides information on their composition and scientific goals, presents the results of preliminary studies performed using a prototype of the instruments. As a result of the studies, the presence of a significant (about 10 m/sec) vertical wind at various altitudes (100 and 250 km) was established, the importance of taking into account the vertical wind to study the vertical dynamics of the charged component was demonstrated. The long-term dynamics of the vertical wind at an altitude of about 100 km has a pronounced seasonal variations and the absence of diurnal variations, whereas the dynamics of the vertical wind at an altitude of 250 km has a pronounced diurnal variations, which is mostly clearly defined in winter. This suggests the presumed presence of vertical circulation cells at various altitude levels. The possibilities of optical stereoscopy and differential image analysis methods are demonstrated, as applied to the study of fast luminous formations and conducting active ground and space experiments to modify Earth's ionosphere. We report the results of the determination of a three-dimensional picture of a long-lived meteor track with the use of two wide-angle cameras. We propose an algorithm that allows us to get a stereo image of events occurring in the upper atmosphere, recorded simultaneously from different observation points. The joint work of the tools of this complex and the development of cooperation with third-party organizations are shown to be a good enough direction for further study of the vertical dynamics of Earth’s upper atmosphere and space weather phenomena.

A 3D bioprinter platform for mechanistic analysis of tumoroids and chimeric mammary organoids

The normal mammary microenvironment can suppress tumorigenesis and redirect cancer cells to adopt a normal mammary epithelial cell fate in vivo. Understanding of this phenomenon offers great promise for novel treatment and detection strategies in cancer, but current model systems make mechanistic insights into the process difficult. We have recently described a low-cost bioprinting platform designed to be accessible for basic cell biology laboratories. Here we report the use of this system for the study of tumorigenesis and microenvironmental redirection of breast cancer cells. We show our bioprinter significantly increases tumoroid formation in 3D collagen gels and allows for precise generation of tumoroid arrays. We also demonstrate that we can mimic published in vivo findings by co-printing cancer cells along with normal mammary epithelial cells to generate chimeric organoids. These chimeric organoids contain cancer cells that take part in normal luminal formation. Furthermore, we show for the first time that cancer cells within chimeric structures have a significant increase in 5-hydroxymethylcytosine levels as compared to bioprinted tumoroids. These results demonstrate the capacity of our 3D bioprinting platform to study tumorigenesis and microenvironmental control of breast cancer and highlight a novel mechanistic insight into the process of microenvironmental control of cancer.

SiRNA-mediated silencing of PAI-1 gene acts as a promoter over the recanalization of endothelial progenitor cells in rats with venous thrombosis.

With the changing lifestyle, venous thrombosis (VT) is becoming increasingly prevalent and poses a burden on the health economy. Endothelial progenitor cells (EPCs) are recruited into resolving VT. We aimed to investigate the effect of plasminogen activator inhibitor 1 (PAI-1) silencing on the recanalization of VT in rat EPCs. EPCs and VT rat models were cultured and treated with negative control-siRNA vector and PAI-1-siRNA vector, respectively. 4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound-healing test, and Matrigel-induced tubular experiment were performed to detect the ability of cell proliferation, migration, and EPCs lumen formation. Immunohistochemistry was used to observe the recanalization of thrombus. The messenger RNA (mRNA) and protein expression of PAI-1 and vascular endothelial growth factor (VEGF) were determined by reverse transcription quantitative polymerase chain reaction and Western blot analysis. PAI-1-siRNA enhances the luminal formation ability of EPCs and significantly promotes EPCs homing. In response to PAI-1 gene silencing, tissues from inferior vena cava displayed reduced mRNA and protein expression of PAI-1, increased VEGF expression as well as promoted lumen-like structures. PAI-1 gene silencing can promote the recanalization of VT by enhancement of the luminal formation ability of rats' EPCs.

Video Recording of Long-Lived Plasmoids near Objects Exposed to Remote and Direct Effects of High-Current Pinch Discharges

The results of observation of plasmoids that were formed during periodic discharge in a flow of liquid, in particular, water, in its surrounding space during discharge and in post-discharge, have been presented. The presence of long-lived luminous formations not only in the air, but also on the surface and above the surface of nearby objects has been noted. The reasons for the appearance of such objects and their properties, which have features not only of macro-, but also micro-objects, such as Dirac’s monopole, have been analyzed.